Spiranthes spiralis (L.) Chevall'de mikorizal çeşitlilik

Yıl 2024, , 52 - 65, 01.03.2024

Vildan Akın Mutlu , İbrahim Özkoç

https://doi.org/10.21597/jist.1403162

Öz

Tehdit altındaki orkidelerin etkili bir şekilde korunması için kökle ilişkili fungusların tanımlanması ve tohum çimlenmesinde etkinliğinin değerlendirilmesi koruma protokolleri oluşturmak için önemlidir. Bu nedenle çalışmamızda Spiranthes spiralis (L.) Chevall 'in kök-ilişkili funguslarının çeşitliliği araştırılmıştır.. Kültüre bağımlı yaklaşımına göre, 'in köklerinden 37 endofitik fungus izole edilmiş, morfolojik ve moleküler tanımlamaları yapılmıştır. kökünde baskın fungus türünün Tulasnella cinsi olduğu belirlenmiştir. Türkiye'de ilk kez köklerinden Thanatephorus fusisporus türü izole edilmiştir. İzole edilen fungusların S. spiralis tohumlarını simbiyotik kültürde çimlendirme etkinlikleri değerlendirilmiştir. Nisan ayında izole edilen VY 25 (Tulasnella) izolatı en yüksek çimlenme oranını (%73.77) göstermiştir. VY 1, VY 4, VY 8, VY 16, VY 25, VY 30, (Tulasnella) izolatları çimlenmeyi ve fide gelişimini teşvik etmiştir. Thanatephorus (%46.79) ve Ceratobasidium (%32.42) cinslerinin ise tohumlarını çimlendirmede etkili olmadığı görülmüştür. Araştırma, fungal partnerin gelişim evrelerine ve/veya aylara göre değiştiğini ortaya koymuştur. Bu çalışma, Türkiye’de köklerinden izole edilen organizmalar için ilk moleküler verileri içermektedir. Aynı zamanda bu sonuçlara göre, tohum çimlenmesini ve bitki büyümesini destekleyen funguslar, nesli tükenmekte olan ılıman orkidelerin korunması ve doğaya yeniden kazandırılması için önerilebilir.

Anahtar Kelimeler

Fungi, Orchids, Tohum çimlenmesi, Simbiyotik kültür, Tulasnella

Proje Numarası

(TUBITAK) (Project No 113Z849 )

Mycorrhizal diversity in Spiranthes spiralis (L.) Chevall

Öz

For effective conservation of threatened orchids, identifying root-associated fungi and assessing their activity in seed germination is important for establishing conservation protocols. Therefore, our study investigated the diversity of Spiranthes spiralis‘s root-associated fungi. According to the culture-dependent approach, 37 endophytic fungi were isolated from the roots and morphologically and molecularly identified. It was determined that the dominant fungal species in the roots was the genus Tulasnella. For the first time in Türkiye, the Thanatephorus fusisporus species was isolated from roots. The germination efficiency of the isolated fungi in the symbiotic culture of S. spiranthes seeds was evaluated. VY 25 (Tulasnella) isolate isolated in April showed the highest germination rate (73.77%). VY 4, VY 18, VY 25, (Tulasnella) isolates promoted germination and seedling development. Thanatephorus (46.79%) and Ceratobasidium (32.42%) were not effective in germinating seeds. The study revealed that the fungal partner varied according to developmental stages and months. This study contains the first molecular data for organisms isolated from roots in Türkiye. According to these results, fungi that promote seed germination and plant growth can be recommended for the conservation and reintroduction of endangered temperate orchids.

Anahtar Kelimeler

Fungi, Orchids, Seed germination, Symbiotic culture, Tulasnella

Destekleyen Kurum

Tubitak

Proje Numarası

(TUBITAK) (Project No 113Z849 )

Teşekkür

We thank Tubitak for their financial contribution.

Kaynakça

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