Evaluation of Toxic Effects of Valeriana alliariifolia Adams Root Extract on SH-SY5Y and PC-12 Cell Lines and Investigation of Antioxidant Activity

Öz

The research examined the toxicity of 80% ethanol extract prepared from the roots of the Valeriana alliariifolia Adams species grown in Turkiye on SH-SY5Y neuroblastoma and neuron-like PC-12 cell lines. The total phenol and flavonoid amounts of the extract were determined by spectrophotometric methods. Antioxidant activity was analyzed by DPPH and ABTS radical scavenging activity determination methods. It was determined that the total phenol content of the extract was 210.42±2.58 mgGAE/gextract and that it could strongly scavenge the DPPH radical in the concentration range of 0.2-4 mg/mL. It has been observed that the extract did not have a toxic effect on SH-SY5Y and PC-12 cells at low concentrations (3.9 and 7.81 µg/mL) and also had lower toxicity on the PC-12 cell line with an IC50 value of 369.68 ± 2.61 µg/mL. The results indicate that Valeriana alliariifolia species may be potential candidates for developing new phytopharmaceuticals and functional ingredients..

Anahtar Kelimeler

• Valeriana alliariifolia
• antioxidants
• pc-12
• sh-sy5y

Proje Numarası

Çalışma proje kapsamında gerçekleştirilmemiştir.

Valeriana alliariifolia Adams Kök Ekstresinin SH-SY5Y ve PC-12 Hücre Hatları Üzerindeki Toksik Etkilerinin Değerlendirilmesi ve Antioksidan Aktivitesinin Araştırılması

Öz

Araştırmada, Türkiye’de yetişen Valeriana alliariifolia Adams türünün köklerinden hazırlanan %80’lik etanol ekstresinin SH-SY5Y nöroblastoma ve nöron benzeri PC-12 hücre hatlarındaki toksisitesi incelenmiştir. Ekstrenin toplam fenol ve flavonoit miktarı spektrofotometrik yöntemlerle belirlenmiştir. Antioksidan aktivitesi, DPPH ve ABTS radikal süpürücü aktivite tayin yöntemleri ile analiz edilmiştir. Ekstrenin, toplam fenol içeriğinin 210.42±2.58 mgGAE/gekstre olduğu, 0.2-4 mg/mL konsantrasyon aralığında ise DPPH radikalini güçlü bir şekilde süpürebildiği belirlenmiştir. Ekstrenin düşük (3.9 ve 7.81 µg/mL) konsantrasyonlarda SH-SY5Y ve PC-12 hücreleri üzerinde toksik etki göstermediği, aynı zamanda 369.68 ± 2.61 µg/mL IC50 değeri ile PC-12 hücre hattında daha düşük toksisiteye sahip olduğu gözlenmiştir. Sonuçlar, Valeriana alliariifolia türünün yeni fitofarmasötikler ve fonksiyonel bileşenler geliştirmek için potansiyel aday olabileceğini göstermektedir.

Anahtar Kelimeler

• Valeriana alliariifolia
• antioksidan
• pc-12
• sh-sy5y

Proje Numarası

Çalışma proje kapsamında gerçekleştirilmemiştir.

Kaynakça

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