The Effects of Iron (Fe+3) on The Expression Levels of Heat Stress Protein Genes in Rat Liver (Rattus norvegicus) Tissue

Yıl 2018, , 255 - 261, 31.03.2018

Atena Ghosıgharehaghajı Hamid Ceylan Orhan Erdoğan

https://doi.org/10.21597/jist.407883

Öz

Iron, one of the most common metals in the world, is important for organisms. It is known that at

high concentrations, it damages especially organs such as liver, pancreas, heart. In our study, the effect of iron

ion on the expression of the 70 kDa HSP gene family which small stress proteins was investigated in Rattus

norvegicus. In this study, iron ion Fe3 + (0,87ppm, 3ppm, 30ppm, 300ppm) was given to 5 different application

groups at different concentrations. At the end of this application period, a cDNA library was formed from the liver

tissues taken from the living body. Using these libraries, changes in expression levels occurring in the HSP70

(Hspa1a, Hspa4, Hspa5) HSP90 (Hsp90aa1) genes were determined by Real-Time PCR method.

Anahtar Kelimeler

Gene expression, hsp, iron ion, Rattus norvegicus, real-time PCR

Demir İyonunun (Fe3+) Sıçan (Rattus norvegicus) Karaciğer Dokusundaki Isı Stres Proteini (Hsp) Genlerinin Ekspresyon Seviyeleri Üzerine Etkisi

Öz

Dünyada en fazla bulunan metallerden birisi olan demir, organizmalar için önemlidir. Yüksek

konsantrasyonlarda özellikle karaciğer, pankreas, kalp gibi organlarda hasar oluşturduğu bilinmektedir.

Çalışmamızda Rattus norvegicus’da demir iyonunun küçük stress proteinleri olan 70 kDa HSP gen ailesinin

ekspresyonu üzerine olan etkisi araştırıldı. Bu çalışma kapsamında 5 farklı uygulama grubuna farklı

konsantrasyonlarda demir iyonu Fe3+ (0,87ppm, 3ppm, 30ppm, 300 ppm) verildi. Bu uygulama süreci sonunda

canlıdan alınan karaciğer dokularından cDNA kütüpanesi oluşturuldu. Yapılan bu kütüpaneler kullanılarak HSP70

(Hspa1a, Hspa4, Hspa5) HSP90 (Hsp90aa1) genlerinde meydana gelen ekspresyon seviyelerindeki değişimler

Real-Time PCR metodu ile tespit edildi.

Anahtar Kelimeler

Demir iyonu, gen ekspresyonu, hsp, Rattus norvegicus, real-time PCR

Kaynakça

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