Antiangiogenic and Cytotoxic Activity of Giresun Tombul Hazelnut (Corylus avellana L.) Oil on Cervix, Breast, and Colon Cancer Cells

Öz

Corylus avellana L. is one of the best quality hazelnut species grown mostly in Giresun province. This present study was realised to evaluate the anticancer and antiangiogenic activities of Giresun Tombul hazelnut oil (0.5, 5 and 50 mg L-1). Cytotoxicity was determined by MTT and lactate dehydrogenase release (LDH) assays on human cervix, colon, and breast cancer cells. Oxidative status was analyzed by total oxidative stress (TOS) and total antioxidant capacity (TAC) assay. Hen’s egg test chorioallantoic membrane (HET-CAM) assay was used to evaluate the anti-angiogenic activity. Our results showed that C. avellana oil had significant anti-proliferative activity against both cervix and colon cancer cells (6.5 ± 0.35 mg L-1 and 26.26 ± 3.15 mg L-1, respectively), with more inhibiting the cervix cancers and caused significant inhibition in LDH release as a linear correlation with MTT results. C. avellana oil significantly increased the TAC status on the cervix and colon cells while causing a significant reduction in TOS levels in a dose-dependent manner. C. avellana oil had anti-angiogenic effectiveness in higher doses. The results indicated that Giresun hazelnut was a promising anticancer agent and however, further studies are needed to reveal its therapeutic effectiveness.

Anahtar Kelimeler

• Antiangiogenic
• anticancer
• antioxidant
• Corylus avellana
• oxidative stress

Giresun Tombul Fındık (Corylus avellana L.) Yağının Serviks, Meme ve Kolon Kanser Hücrelerinde Sitotoksik ve Antianjiyojenik Aktivitesi

Öz

Corylus avellana L. çoğunlukla Giresun’da yetişen dünyanın en kaliteli fındık türlerinden biridir. Bu çalışma, Giresun Tombul fındık yağının (0.5, 5 ve 50 mg L-1) antikanser ve antianjiyojenik aktivitelerini değerlendirmek amacıyla gerçekleştirilmiştir. Sitotoksisite insan serviks, kolon ve meme kanseri hücreleri üzerinde MTT ve laktat dehidrojenaz salım (LDH) analizleri ile belirlenmiştir. Hücrelerin oksidatif durumu, toplam oksidatif stres (TOS) ve toplam antioksidan kapasite (TAK) yöntemleriyle ortaya konulmuştur. Anti-anjiyojenik aktiviteyi değerlendirmek için civciv koryoallantoik membran (HET-CAM) deneyi kullanılmıştır. Sonuçlarımız, C. avellana yağının özellikle serviks kanseri hücrelerinde daha fazla olmak üzere, serviks ve kolon kanseri hücrelerinin (sırasıyla IC 50 = 6.5 ± 0.35 mg L-1 ve IC 50 = 26.2 ± 3.15 mg L-1) canlılığını önemli ölçüde inhibe ettiğini göstermiştir. Bununla birlikte, fındık yağ örneği, LDH salınımında önemli bir inhibisyona yol açarak sitotoksisite ile doğrusal bir korelasyon sergilemiştir. C. avellana yağ örneği konsantrasyona bağlı bir şekilde serviks ve kolon hücrelerinin TAK seviyelerinde önemli bir artışa neden olurken, TOS seviyelerinde istatistiksel olarak önemli azalmaya yol açmıştır. Aynı zamanda, C. avellana yağ örneği yüksek konsantrasyonlarda anti-anjiyojenik etkinlik göstermiştir. Sonuçlar, Giresun fındığının umut verici bir antikanser ajan olduğunu ve bununla birlikte terapötik etkinliğini ortaya koymak için daha ileri çalışmalara ihtiyaç olduğunu göstermiştir.

Anahtar Kelimeler

• Anjiyogenezis
• antikanser
• antioksidan
• Corylus avellana
• oksidatif stres

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