Chemical Priming with β-aminobutyric acid (BABA) for Seedling Vigor in Wheat (Triticum aestivum L.)

Öz

To achieve efficiency in seedling development, the seed must germinate quickly and homogeneously. Pre-sowing applications such as priming are practiced to eliminate or reduce the negative effects of environmental factors through germination and seedling development. This allows to reach a higher germination rate, yield, and desired plant density, especially by protecting against biotic and abiotic stresses. This study was carried out to examine the effects of pre-sowing seed applications on seedling growth and vigor of bread wheat (Triticum aestivum L.). Three different doses of β-amino-n-butyric acid (BABA) were used as seed priming agents and Dimethyl sulfoxide (DMSO) as control groups. The study was carried out according to the completely randomized factorial design with three replications and ten plants per replication. As a result of priming applications, significantly higher values in the number of roots, longest root length, total root length, average root length and shoot fresh weight were obtained compared to control. Our results, for the first time, showed that seed priming with BABA had a promoting effect on many aspects of early seedling growth in bread wheat. This is a preliminary study to understand the mechanism of seed priming and its effects on germination and root growth in wheat. Further studies may shed light on the molecular mechanisms of BABA or other compounds as seed priming agents, benefits for abiotic and biotic stress tolerance, and good stand establishment at the seedling or later stages.

Anahtar Kelimeler

• β-amino-n-butyric acid
• root growth
• seed priming

Kaynakça

1. Acikbas S, Ozyazici MA, Bektas H, (2021). The effect of salinity on root architecture in forage pea (Pisum sativum ssp. arvense L.). Legume Research 44(4): 407-412.
2. Aghbolaghi MA, Ansari OM, Sedghi MO. 2014. The effect of salicylic acid and gibberellic acid on germination characteristics and changes of antioxidant enzymes under accelerated aging in sunflower (Helianthus annuus). Iranian Journal of Seed Science and Technology 4;3(1).
3. Anosheh HP, Sadeghi H, Emam Y, 2011. Chemical priming with urea and KNO 3 enhances maize hybrids (Zea mays L.) seed viability under abiotic stress. Journal of Crop Science and Biotechnology 14(4):289-295.
4. Ashraf MA, Akbar A, Askari SH, Iqbal M, Rasheed R, Hussain I, 2018. Recent advances in abiotic stress tolerance of plants through chemical priming: an overview. Advances in Seed Priming. Rakshit A, Singh HB. Springer Singapore: 51-79.
5. Bagheri MZ. 2014. The effect of maize priming on germination characteristics, catalase and peroxidase enzyme activity and total protein content under salt stress. International Journal of Biosciences 4(2):104-12.
6. Banerjee A, Roychoudhury A, 2018. Seed priming technology in the amelioration of salinity stress in plants. In: Advances in seed priming. Springer, pp 81-93
7. Bektas H, Hohn CE, Waines JG, 2016. Root and shoot traits of bread wheat (Triticum aestivum L.) landraces and cultivars. Euphytica 212(2):297-311.
8. Bose B, Kumar M, Singhal R, Mondal S, 2018. Impact of seed priming on the modulation of physico-chemical and molecular processes during germination, growth, and development of crops. In: A R, H. S (eds) Advances in seed priming. Springer, Singapore.

9. Cao HH, Zhang M, Zhao H, Zhang Y, Wang XX, Guo SS, Zhang ZF, Liu TX, 2014. Deciphering the mechanism of β-aminobutyric acid-induced resistance in wheat to the grain aphid, Sitobion avenae. PloS one 9 (3):e91768.
10. Cohen Y, Vaknin M, Mauch-Mani B, 2016. BABA-induced resistance: milestones along a 55-year journey. Phytoparasitica 44 (4):513-538.
11. Dawood MG, 2018. Stimulating plant tolerance against abiotic stress through seed priming. In: Advances in seed priming. Springer, pp 147-183
12. Demir I, Ellialtioglu S, Tipirdamaz R, 1994. The effect of different priming treatments on reparability of aged eggplant seeds. In International Symposium on Agrotechnics and Storage of Vegetable and Ornamental Seeds 362:205-212.
13. Du YL, Wang Z-Y, Fan JW, Turner NC, Wang T, Li FM, 2012. β-Aminobutyric acid increases abscisic acid accumulation and desiccation tolerance and decreases water use but fails to improve grain yield in two spring wheat cultivars under soil drying. Journal of Experimental Botany 63 (13):4849-4860.
14. Elkoca E, 2007. Priming: ekim öncesi tohum uygulamaları. Atatürk Üniversitesi Ziraat Fakültesi Dergisi 38(1):113-120.
15. FAOSTAT, 2019. Food, Agriculture Organization of the United, Nations. Rome, Italy: Food, Agriculture Organization of the United, Nations.
16. Gallardo K, Job C, Groot SPC, Puype M, Demol H, Vandekerckhove Jl, Job D, 2001. Proteomic analysis of Arabidopsis seed germination and priming. Plant Physiology 126 (2):835-848
17. Godfray HCJ, Beddington JR, Crute IR, Haddad L, Lawrence D, Muir JF, Pretty J, Robinson S, Thomas SM, Toulmin C, 2010. Food security: the challenge of feeding 9 billion people. Science 327 (5967):812-818.
18. Goswami A, Banerjee R, Raha S, 2013. Drought resistance in rice seedlings conferred by seed priming. Protoplasma 250(5):1115-1129.
19. Gökçöl A, Duman İ, 2018. Kapari tohumlarının çimlenmesinin iyileştirilmesinde farklı tohum uygulamalarının etkisinin belirlenmesi. Ege Üniversitesi Ziraat Fakültesi Dergisi 55(4):71-80.
20. Hawkesford MJ, Araus J-L, Park R, Calderini D, Miralles D, Shen T, Zhang J, Parry MAJ, 2013. Prospects of doubling global wheat yields. Food and Energy Security 2 (1):34-48.
21. Heydecker W, Gibbins BM, 1978. The'priming'of seeds. [Conference paper]. Acta Horticulturae (Netherlands). no. 83.
22. Hussain S, Yin H, Peng S, Khan FA, Khan F, Sameeullah M, Hussain HA, Huang J, Cui K, Nie L, 2016. Comparative transcriptional profiling of primed and non-primed rice seedlings under submergence stress. Frontiers in Plant Science 7. 1125.
23. Jelali N, Youssef RB, Boukari N, Zorrig W, Dhifi W, Abdelly C, 2021. Salicylic acid and H2O2 seed priming alleviates Fe deficiency through the modulation of growth, root acidification capacity and photosynthetic performance in Sulla carnosa. Plant Physiology and Biochemistry 159:392-399.
24. Jian H, Wang J, Wang T, Wei L, Li J, Liu L, 2016. Identification of rapeseed microRNAs involved in early stage seed germination under salt and drought stresses. Frontiers in Plant Science 7(658).
25. Jisha KC, Puthur JT, 2016. Seed priming with BABA (β-amino butyric acid): a cost-effective method of abiotic stress tolerance in Vigna radiata (L.) Wilczek. Protoplasma 253(2):277-289.
26. Karakurt H, Aslantaş R, Eşitken A, 2010. Tohum çimlenmesi ve bitki büyümesi üzerinde etkili olan çevresel faktörler ve bazı ön uygulamalar. Uludağ Üniversitesi Ziraat Fakültesi Dergisi 24(2):115-128.
27. Kubala S, Garnczarska M, Wojtyla Ł, Clippe A, Kosmala A, Żmieńko A, Lutts S, Quinet M, 2015. Deciphering priming-induced improvement of rapeseed (Brassica napus L.) germination through an integrated transcriptomic and proteomic approach. Plant Science 231:94-113.
28. Kulak M, Jorrín-Novo JV. Romero-Rodriguez MC, Yildirim ED, Gul F, Karaman S, 2021. Seed priming with salicylic acid on plant growth and essential oil composition in basil (Ocimum basilicum L.) plants grown under water stress conditions. Industrial Crops and Products 161:113235.
29. Lal SK, Kumar S, Sheri V, Mehta S, Varakumar P, Ram B, Borphukan B, James D, Fartyal D, Reddy MK, 2018. Seed priming: an emerging technology to impart abiotic stress tolerance in crop plants. In: Advances in Seed Priming. Rakshit A, Singh HB. Springer Singapore: pp 41-50
30. Lara TS, Lira JM, Rodrigues AC, Rakocevi M, Alvarenga AA. 2014. Potassium nitrate priming affects the activity of nitrate reductase and antioxidant enzymes in tomato germination. Journal of Agricultural Science 6(2):72.
31. Li R, He J, Xie H, Wang W, Bose SK, Sun Y, Hu J, Yin H, 2019. Effects of chitosan nanoparticles on seed germination and seedling growth of wheat (Triticum aestivum L.). International Journal of Biological Macromolecules 126:91-100.
32. Martínez-Aguilar K, Hernández-Chávez JL, Alvarez-Venegas R, 2021. Priming of seeds with INA and its transgenerational effect in common bean (Phaseolus vulgaris L.) plants. Plant Science 305:110834. Mostek A, Börner A, Weidner S, 2016 Comparative proteomic analysis of β-aminobutyric acid-mediated alleviation of salt stress in barley. Plant Physiology and Biochemistry 99:150-161.
33. Nouman W, Siddiqui MT, Basra SM, Afzal I, Rehman HU. 2012. Enhancement of emergence potential and stand establishment of Moringa oleifera Lam. by seed priming. Turkish Journal of Agriculture and Forestry 36(2):227-35.
34. Oka Y, Cohen Y, 2001. Induced resistance to cyst and root-knot nematodes in cereals by DL-β-amino-n-butyric acid. European Journal of Plant Pathology 107 (2):219-227
35. Özkaynak E, Orhan Y, Kargın İ, Tuncel M, 2020. Biber ve domates tohumlarında organik priming uygulamaları. Black Sea Journal of Agriculture, 3(4):301-307.
36. Paul S, Roychoudhury A, 2017. Effect of seed priming with spermine/spermidine on transcriptional regulation of stress-responsive genes in salt-stressed seedlings of an aromatic rice cultivar. Plant Gene 11:133-142.
37. Ray DK, Mueller ND, West PC, Foley JA, 2013. Yield trends are insufficient to double global crop production by 2050. PLoS ONE 8:e66428.
38. Rueden CT, Schindelin J, Hiner MC, DeZonia BE, Walter AE, Arena ET, Eliceiri KW, 2017. ImageJ2: ImageJ for the next generation of scientific image data. BMC Bioinformatics 18(1):1-26.
39. Salah SM, Yajing G, Dongdong C, Jie L, Aamir N, Qijuan H, Weimin H, Mingyu N, Jin H (2015) Seed priming with polyethylene glycol regulating the physiological and molecular mechanism in rice (Oryza sativa L.) under nano-ZnO stress. Scientific Reports 5 (1).
40. Soeda Y, Konings MCJM, Vorst O, van Houwelingen AMML, Stoopen GM, Maliepaard CA, Kodde J, Bino RJ, Groot SPC, van der Geest AHM, 2005. Gene expression programs during Brassica oleracea seed maturation, osmopriming, and germination are indicators of progression of the germination process and the stress tolerance level. Plant physiology 137 (1):354-368.
41. Steel RGD, Torrie JH, Dickey DA, 1997. Principles and procedures of statistics: a biometrical approach, McGraw-Hill, New York.
42. Taylor AG, 1997. Seed storage germination and quality. In: Wien HC, (ed.) The physiology of vegetable Crops. CAB International, Wallingford, U.K, 1-36.
43. Wahid A, Perveen M, Gelani S, Basra SMA, 2007. Pretreatment of seed with H2O2 improves salt tolerance of wheat seedlings by alleviation of oxidative damage and expression of stress proteins. Journal of Plant Physiology 164 (3):283-294.
44. Yin X, Liu S, Qin Y, Xing R, Li K, Yu C, Chen X, Li P, 2021. Metabonomics analysis of drought resistance of wheat seedlings induced by β-aminobutyric acid-modified chitooligosaccharide derivative. Carbohydrate Polymers 272:118437.
45. Zhu J, Mickelson SM, Kaeppler SM, Lynch JP, 2006. Detection of quantitative trait loci for seminal root traits in maize (Zea mays L.) seedlings grown under differential phosphorus levels. Theoretical and Applied Genetics 113(1):1-10.
46. Zimmerli L, Hou BH, Tsai CH, Jakab G, Mauch‐Mani B, Somerville S, 2008. The xenobiotic β‐aminobutyric acid enhances Arabidopsis thermotolerance. The Plant Journal 53(1):144-156.