Defne (Laurus nobilis L.) ve Zahter (Thymbra spicata L.) Uçucu Yağlarının Farklı Konsantrasyonlarda Rhyzopertha dominica (Coleoptera: Bostrichidae) ve Oryzaephilus surinamensis (Coleoptera: Silvanidae) Üzerine Fumigant Etkilerinin Araştırılması

Yıl 2019, Cilt: 9 Sayı: 3, 1234 - 1242, 01.09.2019

Mehmet Ali Kırpık Pinar Aksu Kılıçle Yağmur Yıldız Asker

https://doi.org/10.21597/jist.488946

Cited By: 3

Öz

Bu çalışmada Defne (Laurus nobilis L.) ve Zahter (Thymbra spicata L.) uçucu yağlarının farklı konsantrasyonlarda Rhyzopertha dominica (Coleoptera: Bostrichidae) ve Oryzaephilus surinamensis (Coleoptera: Silvanidae) üzerine fumigant etkileri araştırılmıştır. Bitkilerin yaprak kısımları uygun şartlarda kurutulup su buhar distilasyonu ile uçucu yağları elde edildi. L. nobilis (12.5, 25, 50, 100, 150 μl L-1) ve T. spicata (12.5, 25, 50, 100, 150 μl L-1) aseton (150 μl L-1) ile çözülerek R. dominica ve O. surinamensis türleri için belirlenen süre ve dozlarda uygulandı. R. dominica için kurulan deney düzeneğinde, L. nobilis için uygulanan ilk doz herhangi bir madde uygulanmayan negatif kontrolle benzer sonuçlar gösterirken, doz ve zaman artışına bağlı olarak ölüm sayılarının arttığı görüldü ve 100 μl L-1 lik dozda 24 saat sonunda %100 fumigant toksisite kaydedildi. Aynı böcek türü için T. spicata uygulaması sonucunda da benzer sonuçlar görülürken en yüksek doz 24 saat sonunda %100 fumigant toksisite gösterdi. Diğer bir depo zararlısı olan O. surinamensis için de aynı bitki uçucu yağları ve dozları kullanılarak bu tür içinde benzer sonuçlar kaydedildi. Elde edilen veriler neticesinde önemli depo zararlılarından olan R. dominica ve O. surinamensis için bitki uçucu yağ ve bileşenleri sentetik pestisitlerin kullanımının azaltılması amacıyla kullanılabilir.

Anahtar Kelimeler

Laurus nobilis L., Thymbra spicata L., Rhyzopertha dominica, Oryzaephilus surinamensis, Fumigant etki

Investigation of Fumigant Effects of Laurel (Laurus nobilis L.) and Zahter (Thymbra spicata L.) Essential Oils in Different Concentrations on Rhyzopertha dominica (Coleoptera: Bostrichidae) and Oryzaephilus surinamensis (Coleoptera: Silvanidae)

Öz

In this study, fumigant effects were investigated of Laurel (Laurus nobilis L.) and Zahter (Thymbra spicata L.) essential oils in different concentrations on Rhyzopertha dominica (Coleoptera: Bostrichidae) and Oryzaephilus surinamensis (Coleoptera: Silvanidae). For each insect species separately for R. dominica and O. surinamensis species, 12 groups were formed in each group as 20 insects. These groups were the control group, solvent control group (150 μl L-1) include five different doses for L. nobilis. essential oils group (12.5, 25, 50, 100, 150 μl L-1) include five different doses for T. spicata essential oils group (12.5, 25, 50, 100, 150 μl L-1). In the experimental setup for R. dominica, familiar results were obtained from L. nobilis with negative group, and solvent solution group, but mortality increased depend on increase in time and concentration. At the end of 24 hours, 100% fumigant toxicity was recorded in 100 μl L-1 solution. Similar results were obtained with the T. spicata application for the same insect species, with the highest concentration showing 100% fumigant toxicity after 24 hours. Similar results were recorded for O. surinamensis another storage pest, using the same plant essential oils and concentrations. As a result of the data obtained from the plant essential oil and its components, for R. dominica and O. surinamensis can be used instead of synthetic pesticides.

Anahtar Kelimeler

Laurus nobilis L., Thymbra spicata L., Rhyzopertha dominica, Oryzaephilus surinamensis, Oryzaephilus surinamensis, Fumigant effect

Kaynakça

• Ahmed S, Grainge M, 1988. Handbook of Plants with Pest Control Properties. John Wiley & Sons Limited, New York, pages 470.
• Auamcharoen W, Chandrapatya A, Kijjoa A, Kainoh Y, 2012. Toxicity and Repellency Activities of the Crude Methanol Extract of Duabanga grandiflora (Lythraceae) Against Sitophilus oryzae (Coleoptera: Curculionidae). Pakistan Journal of Zoology, 44 (1): 227-232.
• Ayvaz A, Sagdic O, Karabörklü S, Oztürk I, 2010. Insecticidal activity of the essential oils from different plants against three stored-product insects. Journal of Insect Science, 10: 21, doi: 10.1673/031.010.2101.
• Ayyıldız T, Karaca İ, 2018. Bazı Biyolojik Preparatların Sitophilus granarius (Coleoptera: Curculionidae) Erginlerine Etkileri. Süleyman Demirel Üniversitesi Fen Bilimleri Enstitüsü Dergisi, 22 (2): 671-678.
• Başer KHC, 2001. Her Derde Deva Bir Bitki Kekik, Bilim ve Teknik Dergisi. 402:74-77.
• Belmain SR, Neal GE, Ray DE. Golop P, 2001. Insecticidal and vertebrate toxicity associated with ethnobotanicals used as postharvest protectants in Ghana. Food and Chemical Toxicology, 39: 287-291.
• Boxall RA, 2001. Post-harvest losses to insects - a world overview. International biodeterioration and biodegadation, 48: 137-152.
• Champ BR, Dyte CE, 1976. FAO global servey of pesticide susceptibility of stored grain pests. FAO plant protection bulletin, 25: 49-67.
• Eliopoulos PA, Hassiotis CN, Andreadis SS, Porichi AE, 2015. Fumigant Toxicity of Essential Oils from Basil and Spearmint Against Two Major Pyralid Pests of Stored Products. Journal of Economic Entomology, 108 (2): 805-10. doi: 10.1093/jee/tov029.
• Emekçi M, Ferizli AG, 2000. Current Status of Stored Product Protection in Turkey. IOBC/WPRS Study Group Integrated Protection of Stored Products, Berlin, IOBC wprs Bulletin, Vol. 23 (10) 2000: 39-45 1999.
• Erden Ü, 2005. Akdeniz Defnesi (Laurus nobilis L.)’nde Mevsimsel Varyabilite Ve Optimal Kurutma Yöntemlerinin Araştırılması. Çukurova Üniversitesi, Fen Bilimleri Enstitüsü, Tarla Bitkileri Anabilim Dalı, 47 s. Adana.
• Ferizli AG, Emekci M, 2010. Depolanmış ürün zararlılarıyla savaşım, sorunlar ve çözüm yolları. TMMOB Ziraat Mühendisleri Odası Ziraat Mühendisliği VII. Teknik Kongesi 11-15 Ocak 2010 Ankara, Bildiriler Kitabı 2, 579-587.
• Golop P, Moss C, Dales M, Fidgen A, Evans J, Gudrups I, 1999. The use of spices and medicinals as bioactive protectants for grains. FAO Agricultural Services Bulletin 137, FAO, Rome, Italy.
• Hashem MY, Ahmed SS, El-Mohandes MA, Gharib MA, 2012. Susceptibility of different life stages of saw-toothed grain beetle Oryzaephilus surinamensis (L.) (Coleoptera: Silvanidae) to modified atmospheres enriched with carbon dioxide. Journal of Stored Product Research, 48: 46-51.
• Hills DS, 2002. Pests of Stored Foodstuffs and Their Control. Kluwer Academic Publishers, The Netherlands. 496 pp.
• Isman MB, 2006. Botanical insecticides, deterrents and repellents in modern agriculture and an increasingly regulated world. Annu. Rev. Ent., 51: 45-66. Journal of Economic Entomology, 108 (2): 805-10. doi: 10.1093/jee/tov029.
• Karabörklü S, Ayvaz A, Yilmaz S, Akbulut M, 2011. Chemical composition and fumigant toxicity of some essential oils against Ephestia kuehniella. Journal of Economic Entomology, 104 (4): 1212-1219.
• Kaurinovic B, Popovic M, Vlaisavljevic S, 2010. In vitro and in vivo effects of Laurus nobilis L. leaf extracts. Molecules, 15: 3378- 3390.
• Kéita SM, Vincent C, Schmit J, Arnason JT, Bélanger A, 2001. Efficacy of essential oil of Ocimum basilicum L. and O. gratissimum L. applied as an insecticidal fumigant and powder to control Callosobruchus maculatus (Fab.). Journal of Stored Products Research, 37 (4): 339-349.
• Marques A, Teixeira B, Nunes ML, 2016. Bay laurel (Laurus nobilis) oils. Chapter 26, p: 239- 246. (Editor, Preedy, V.R.: Essential Oils in Food Preservation, Flavor and Safety, Academic Press, 896 pp. London, UK.
• Nawrot J, Harmatha J, 1994. Natural products as antifeedants against stored product insects. PostHarv. News Inform, 5: 17-21.
• Prakash A, Rao J, 1996. Botanical Pesticides in Agriculture. CRC Pres, Lewis Publishers, pages 461.
• Richard TR, Bruce ET, 1990. Pesticide Resistance in arthropods. Chapman and Hall, pp.303, New York.
• Rossiter LC, Gunning RV, Rose HA, 2001. The use of polyacrylamide gelelectrophoresis for the investigation and detection of fenitrothion and chlorpyrifos-methyl resistance in Oryzaephilus surinamensis (Coleoptera: Silvanidae). Pesticide, Biochemistry, Physiology, 69: 27-34.
• Sarı AO, Oğuz B, 2002. Kekik, Ege Tarımsal Araştırma Enstitüsü Yayınları No.:108. Menemen, İzmir.
• Shaaya E, Kostjucovski M, Eilberk J, Sukprakarn C, 1997. Plant oils as fumigants and contact insecticides for the control of stored product insect. Journal of Stored Product Research, 33: 7-15.
• Srivastava C, Singh D, 2002. Study of phosphine resistance in Rhyzopertha dominica and Callosobruchus maculates. Indian Journal of Entomology, 64: 377-378.
• Subramanyam B, Hagstrum DW, 1995. Resistance measurement and management. In: Integrated management of insects in stored products (eds. B. Subramanyam and D.W. Hagstrum). Marcel Dekker, pp. 331-397, New York.
• TAGEM; 2008. Tarımsal Araştırmalar ve Politikalar Genel Müdürlüğü; Zirai mücadele teknik talimatları, 1: 212-226.
• White NDG, Leesch JG, 1995. Chemical control. In: Integrated management of insects in stored products (eds. B. Subramanyam and D.W. Hagstrum). Marcel Dekker, pp. 287-330, New York.
• Yanar D, Düzdemir O, 2012. Bazı Bitki Ekstraktlarının ve Bitkisel Preparatların Fasulye Tohum Böceğine (Acanthoscelides obtectus (Say.)) Olan Etkisi, Tarım Bilimleri Araştırma Dergisi, 6 (1): 36-40.
• Yankanchi SR, Gadache AH, 2010. Grain protectant efficacy of certain plant extracts against rice weevil, Sitophilus oryzae L. (Coleoptera: Curculionidae). Journal of Biopesticides, 3: 511-513.