Yıl 2018, Cilt 1 , Sayı 2, Sayfalar 66 - 71 2018-12-26

Antioxidant and Antimicrobial Activity of Sea Cucumber (Holothuria tubulosa, Gmelin 1791) Extracts
Deniz Hıyarı (Holothuria tubulosa, Gmelin 1791) Ekstraktlarının Antioksidan ve Antimikrobiyal Aktivitesi

İbrahim Ender KÜNİLİ [1] , Fatma Arık ÇOLAKOĞLU [2]


Holothuria tubulosa is an economic and most harvested sea cucumber species found in Turkey seas. Sea cucumbers are known to be sources of some of bioactive components which are play important roles in human health. In this study, it was aimed to determine the antioxidant and antimicrobial activity of H. tubulosa. The extracts of H. tubulosa were obtained with using different solvents such as, acetonitrile/trifloroacetic acid, methanol, and water/methanol. According to results, antioxidant activity of the extracts was lower than the reference antioxidant agents at all concentrations. However, the activity at higher concentrations of extracts was found to be closer to reference agents. Bacteriostatic effect was observed after the concentration of 12 µg/ml for all bacteria except Bacillus cereus, which has also shown resistance to reference antibiotic. As a consequence, H. tubulosa found in Turkey seas may be regarded as potential source of natural antioxidant agent.

Holothuria tubulosa Türkiye denizlerinde bulunan ekonomik ve en çok hasat edilen deniz hıyarı türlerindendir. Deniz hıyarları insan sağlığı için önemli olan bioaktif bileşiklerin kaynağı olarak bilinmektedir. Bu çalışmada H. tubulosa’nın antioksidan ve antimikrobiyal etkisinin belirlenmesi amaçlanmıştır. H. tubulosa ekstraktları, asitontril/trifloroasetik asit, methanol ve su/metanol gibi farklı çözücüler kullanılarak elde edilmiştir. Sonuçlara göre, ekstraktların antioksidan aktiviteleri, referans antioksidanlara göre tüm konsantrasyonlarda daha düşük belirlenmiştir. Ancak, yüksek konsantrasyonlarda, ekstraktların antioksidan aktivitesi referans antioksidanlara yakın derecede bulunmuştur. Bakteriyostatik etki 12 µg/ml konsantrasyonundan sonra belirlenmiş, ancak bu etki hiçbir konsantrasyon ve ekstrakta, Bacillus cereus için gözlemlenmemiştir. Sonuç olarak, Türkiye denizlerinde bulunan H. tubulosa’nın, potansiyel doğal antioksidan kaynağı olabileceği düşünülmektedir.  

  • Aleman, A., Santin, E.P., Juchereau, B., Arnaudin, I., Guillen, M.C.G., Montero, P. (2011). Squid gelatin hydrolysates with antihypertensive, anticancer and antioxidant activity. Food Research International, 44, 1044-1051. https://doi.org/10.1016/j.foodres.2011.03.010.
  • Althunibat, O.Y., Ridzwan, H., Taher, M., Daud, J.M., Ichwan, S.J.A., Qaralleh, H. (2013). Antioxidant and Cytotoxic Properties of Two Sea Cucumbers, Holothuria edulis Lesson and Stichopus horrens Selenka. Acta Biologica Hungarica, 64(1), 10-20. https://doi.org/10.1556/abiol.64.2013.1.2.
  • Aydın, M. (2008). The commercial sea cucumbers fishery in Turkey. SPC Beche de Mer Informaiton Bulletin, 28, 40–43.
  • Aydın, M., Sevgili, H., Tufan, B., Emre, Y., Köse, S. (2011). Proximate composition and fatty acid profile of three different fresh and dried commercial sea cucumbers from Turkey. International Jorunal of Food Science and Technology, 46, 500-508. https://doi.org/10.1111/j.1365-2621.2010.02512.x.
  • Bougatef, A., Balti, R., Haddar, A., Jellouli, K., Souissi, N., Nasri, M. (2012). Protein hydrolysates from Bluefin Tuna (Thunnus thynnus) heads as influenced by the extent of enzymatic hydrolysis. Biotechnology and Bioprocess Engineering, 17, 841–852. https://doi.org/10.1007/s12257-012-0053-y.
  • Çaklı, Ş., Cadun, A., Kişla, D., Dinçer, T. (2004). Determination of quality characteristics of Holothuria tubulosa, (Gmelin, 1788) in Turkish sea (Aegean Region) depending on sun drying process step used in Turkey. Journal of Aquatic Food Product Technology, 13, 69–78. https://doi.org/10.1300/j030v13n03_07.
  • Devienne, K.F., Raddi, M.S.G. (2002). Screening for antimicrobial activity of natural products using a microplate photometer. Brazilian Journal of Microbiology, 33, 166-168. https://doi.org/10.1590/s1517-83822002000200014.
  • Dobretsov, S., Al-Mammari, I.M., Soussi, B. (2009). Bioactive Compounds from Omani Sea Cucumbers. Agricultural and Marine Sciences, 14, 49-53. https://doi.org/10.24200/jams.vol14iss0pp49-53.
  • Esmat, A.Y., Said, M.M., Soliman, A.A., El-Masry, K.S.H., Badiea, E.A. 2013. Bioactive compounds, antioxidant potential, and hepatoprotective activity of sea cucumber (Holothuria atra) against thioacetamide intoxication in rats. Basic nutritional investigation, 29, 258-267. https://doi.org/10.1016/j.nut.2012.06.004.
  • Liu, X., Sun, Z., Zhang, M., Meng, X., Xia, X., Yuan, W., Xue, F., Liu, C. (2012). Antioxidant and antihyperlipidemic activities of polysaccharides from sea cucumber Apostichopus japonicus. Carbohydrate Polymers, 90, 1664-1670. https://doi.org/10.1016/j.carbpol.2012.07.047.
  • Mashjoor, S., Yousefzadi, M., Pishevarzad, F. (2018). In vitro biological activities of holothurians edible sea cucumbers in the Persian Gulf. Indian Journal of Geo Marine Sciences, 47(07), 1518-1526. http://nopr.niscair.res.in/handle/123456789/44598.
  • Molyneux, P. (2004). The use of the stable free radikal diphenylpicryllhydrazly (DPPH) for estimating antioxidant activity. Songklanakarin Journal of Science and Technology, 26, 211-219.
  • Murniasih, T., Putra, M.Y. and Pangestuti, R. (2015). Antioxidant Capacities of Holothuria Sea Cucumbers. Annales Bogorienses, 19, 2. http://dx.doi.org/10.14203/ann.bogor.2015.v19.n2.21-26.
  • Nobsathian, S., Tuchinda, P., Sobhon, P., Tinikul, Y., Poljaroen, J., Tinikul, R., Sroyraya, M., Poomton, T., Chaichotranunt, S. (2017). An antioxidant activity of the whole body of Holothuria scabra. Chemical and Biological Technologies in Agriculture, 4, 4. https://doi.org/10.1186/s40538-017-0087-7.
  • Öztoprak, B., Doğan, A., Dağlı E. (2014). Checklist of Echinodermata from the coasts of Turkey. Turkish Journal of Zoology, 38, 892-900. https://doi.org/10.3906/zoo-1405-82.
  • Peng, X., Xiong, Y.L., Kong, B. (2009). Antioxidant activity of peptide fractions from whey protein hydrolysates as measured by electron spin resonance. Food Chemistry, 113, 196-201. https://doi.org/10.1016/j.foodchem.2008.07.068.
  • Qi, H., Li, N., Zhao, X., Xu, Z., Qi, L. (2016). Physicochemical Properties and Radical Scavenging Capacities of Pepsin-Solubilized Collagen from the Body Wall of Starfish (Asterina pectinifera). Journal of Aquatic Food Product Technology, 26(4), 376-389. https://doi.org/10.1080/10498850.2016.1186768.
  • Sun, H.H., Mao, W.J., Chen, Y., Guo, S.D., Li, H.Y., Qi, X.H., Chen, Y.L., Xu, J. (2009). Isolation, chemical characteristics and antioxidant properties of the polysaccharides from marine fungus Penicillium sp. F23-2. Carbohydrate Polymers, 78, 117-124. https://doi.org/10.1016/j.carbpol.2009.04.017.
Birincil Dil en
Konular Deniz ve Tatlı Su Biyolojisi
Bölüm Kısa Bildiri
Yazarlar

Orcid: 0000-0003-2830-6979
Yazar: İbrahim Ender KÜNİLİ (Sorumlu Yazar)
Ülke: Turkey


Yazar: Fatma Arık ÇOLAKOĞLU

Tarihler

Yayımlanma Tarihi : 26 Aralık 2018

Bibtex @araştırma makalesi { jmsf486335, journal = {Çanakkale Onsekiz Mart University Journal of Marine Sciences and Fisheries}, issn = {}, eissn = {2651-5326}, address = {Çanakkale Onsekiz Mart Üniversitesi, Deniz Bilimleri ve Teknolojisi Fakültesi, Terzioğlu Kampüsü, 17100, Merkez/Çanakkale}, publisher = {Çanakkale Onsekiz Mart Üniversitesi}, year = {2018}, volume = {1}, pages = {66 - 71}, doi = {}, title = {Antioxidant and Antimicrobial Activity of Sea Cucumber (Holothuria tubulosa, Gmelin 1791) Extracts}, key = {cite}, author = {KÜNİLİ, İbrahim Ender and ÇOLAKOĞLU, Fatma Arık} }
APA KÜNİLİ, İ , ÇOLAKOĞLU, F . (2018). Antioxidant and Antimicrobial Activity of Sea Cucumber (Holothuria tubulosa, Gmelin 1791) Extracts. Çanakkale Onsekiz Mart University Journal of Marine Sciences and Fisheries , 1 (2) , 66-71 . Retrieved from https://dergipark.org.tr/tr/pub/jmsf/issue/41646/486335
MLA KÜNİLİ, İ , ÇOLAKOĞLU, F . "Antioxidant and Antimicrobial Activity of Sea Cucumber (Holothuria tubulosa, Gmelin 1791) Extracts". Çanakkale Onsekiz Mart University Journal of Marine Sciences and Fisheries 1 (2018 ): 66-71 <https://dergipark.org.tr/tr/pub/jmsf/issue/41646/486335>
Chicago KÜNİLİ, İ , ÇOLAKOĞLU, F . "Antioxidant and Antimicrobial Activity of Sea Cucumber (Holothuria tubulosa, Gmelin 1791) Extracts". Çanakkale Onsekiz Mart University Journal of Marine Sciences and Fisheries 1 (2018 ): 66-71
RIS TY - JOUR T1 - Antioxidant and Antimicrobial Activity of Sea Cucumber (Holothuria tubulosa, Gmelin 1791) Extracts AU - İbrahim Ender KÜNİLİ , Fatma Arık ÇOLAKOĞLU Y1 - 2018 PY - 2018 N1 - DO - T2 - Çanakkale Onsekiz Mart University Journal of Marine Sciences and Fisheries JF - Journal JO - JOR SP - 66 EP - 71 VL - 1 IS - 2 SN - -2651-5326 M3 - UR - Y2 - 2018 ER -
EndNote %0 Çanakkale Onsekiz Mart University Journal of Marine Sciences and Fisheries Antioxidant and Antimicrobial Activity of Sea Cucumber (Holothuria tubulosa, Gmelin 1791) Extracts %A İbrahim Ender KÜNİLİ , Fatma Arık ÇOLAKOĞLU %T Antioxidant and Antimicrobial Activity of Sea Cucumber (Holothuria tubulosa, Gmelin 1791) Extracts %D 2018 %J Çanakkale Onsekiz Mart University Journal of Marine Sciences and Fisheries %P -2651-5326 %V 1 %N 2 %R %U
ISNAD KÜNİLİ, İbrahim Ender , ÇOLAKOĞLU, Fatma Arık . "Antioxidant and Antimicrobial Activity of Sea Cucumber (Holothuria tubulosa, Gmelin 1791) Extracts". Çanakkale Onsekiz Mart University Journal of Marine Sciences and Fisheries 1 / 2 (Aralık 2018): 66-71 .
AMA KÜNİLİ İ , ÇOLAKOĞLU F . Antioxidant and Antimicrobial Activity of Sea Cucumber (Holothuria tubulosa, Gmelin 1791) Extracts. JMSF. 2018; 1(2): 66-71.
Vancouver KÜNİLİ İ , ÇOLAKOĞLU F . Antioxidant and Antimicrobial Activity of Sea Cucumber (Holothuria tubulosa, Gmelin 1791) Extracts. Çanakkale Onsekiz Mart University Journal of Marine Sciences and Fisheries. 2018; 1(2): 71-66.