NaCl Kaynaklı Tuz Stresinin Lotus corniculatus L. cv. 'Leo'nun Çimlenmesi ve İlk Fide Büyümesi Üzerindeki Etkisi

Öz

Lotus corniculatus L. orta derecede tuza toleranslı, tarımsal ve ekonomik açıdan önemli çok yıllık baklagil yem türlerinden biridir. Bitkilerde aynı türün çeşitlerinin bile tuzluluğa farklı tepkiler verdiği iyi bilinmektedir. Ancak tuzluluk gibi abiyotik stres faktörlerinden etkilenen marjinal tarım arazilerinin kullanımında ak üçgül (Trifolium repens) ve yonca (Medicago sativa L.) gibi diğer yem bitkilerine göre daha avantajlı olan L. corniculatus ve çeşitlerinin tuz tepkisi üzerine yapılan çalışmalar sınırlıdır. Tuz stresi altında, bitkinin hayatta kalmasıyla doğrudan ilgili olan bitki yaşam döngüsünün en önemli aşamaları tohumların çimlenmesi, büyümesi ve canlılığıdır. Bu nedenle bu çalışma, diğer çeşitlere göre daha yüksek tanen içeriğine sahip olduğu bilinen L. corniculatus çeşidi 'Leo'nun NaCl türevli tuz stresi altındaki in vitro çimlenme ve büyüme tepkilerini belirlemek amacıyla yapılmıştır. Bu amaçla L. corniculatus tohumları 0, 40 ve 80 mM NaCl içeren MS (Murashige ve Skoog/Gamborg) besiyerinde 14 gün kültüre edilmiştir. Tohum çimlenme yüzdesi, ortalama çimlenme süresi, çimlenme oranı indeksi, sürgün-kök uzunluğu, kök-sürgün uzunluğu oranı, sürgün-kök yaş-kuru ağırlığı, sürgün-kök kuru maddesi, kök-sürgün kuru madde oranı, sürgün- kök su içeriği ve fide canlılık indeksi parametreleri ölçülmüştür. Araştırma sonuçlarına göre, uygulanan NaCl uygulamalarında çimlenme yüzdesi değişmemiş ancak çimlenme hızı azalmıştır. Bununla birlikte sürgün uzunluğu azalmış, kök uzunluğu artmıştır. İstatisitiki açıdan sürgün ve kök yaş-kuru ağırlığında önemli bir değişme olmamasına rağmen, 80 mM NaCl uygulamasında her ikisinde de azalış olmuştur. Sürgün ve kök kuru madde oranı artmış su içeriği azalmıştır. Ayrıca, fide canlılık indeksi de azalmıştır. 40 mM NaCl uygulamasında ise düşük doz olumlu etkisi ile sürgün yaş-kuru ağırlık, kuru madde oranı ve fide canlılık indeksinde artış olmuştur. Bu çalışma kapsamında önemli bir yem bitkisi olan L. corniculatus (kültivar 'Leo') için tuz stresi altında çimlenme ve fide gelişimi bakımından kapsamlı bilgi sunulmuştur.

Anahtar Kelimeler

• Lotus corniculatus L.
• Tuzluluk
• Çimlenme
• In vitro
• İlk fide büyüme aşaması

Effect of NaCl-induced Salt Stress on Germination and Initial Seedling Growth of Lotus corniculatus L. cv. 'Leo'

Öz

Lotus corniculatus L. is one of the agronomically and economically important perennial legume forage species with moderately salt-tolerant. It is well known that even the cultivars of the same species in plants have different responses in salinity. However, studies on the salt response of L. corniculatus and its cultivars, which are more advantageous than other forage crops such as white clover (Trifolium repens) and alfalfa (Medicago sativa L.), in the use of marginal agricultural lands affected by abiotic stress factors such as salinity, are limited. Under salt stress, the most crucial phases of the plant life cycle that are directly related to the survival of the plant are seed germination, growth, and vigour. Therefore, this study was carried out to determine the germination and growth responses of L. corniculatus cultivar 'Leo', which is known to have higher tannin content than other cultivars, under NaCl-derived salt stress in vitro. For this purpose, L. corniculatus seeds were cultured in MS (Murashige and Skoog/Gamborg) medium containing 0, 40, and 80 mM NaCl for 14 days. Seed germination percentage, mean germination time, germination rate index, shoot-root length, root to shoot length ratio, shoot-root fresh dry weight, shoot-root dry matter, the ratio of root to shoot dry matter, shoot-root water content and seedling vigour index parameters were measured. According to the results of the research, the germination percentage did not change in the applied NaCl treatments, but the germination rate decreased. However, shoot length decreased and root length increased. Although there was no statistically significant change in shoot and root fresh-dry weight, both decreased in 80 mM NaCl treatment. The shoot and root dry matter increased and the water content decreased. Also, the seedling viability index decreased. In 40 mM NaCl treatment, on the other hand, there was an increase in shoot fresh-dry weight, dry matter ratio and seedling viability index with the positive effect of low dose. Within the scope of this study, comprehensive information was presented for L. corniculatus (cultivar 'Leo'), an important forage plant, in terms of germination and seedling growth under salt stress.

Anahtar Kelimeler

• Lotus corniculatus L.
• Salinity
• Germination
• In vitro
• Initial seedling growth stage

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