The Effect of Biochar Material Doses on Elimination of Salt Stress on Peanut (Arachis hypogaea L.) Seedlings

Öz

Salinity is one of the important problems that reduce plant growth and agricultural productivity in our country as well as worldwide. In general, plants are more sensitive to salinity at the seedling stage than at the germination stage. It has been reported that the use of biochar provided some benefits on plant growth and soil properties. This study was conducted in 2021 by using different ratios of NaCl salt and biochar material from the orange tree, In order to investigate the effect on the development of NC-7 peanut, it was carried out as a pot experiment in the field conditions at Bingöl University Faculty of Agriculture, Department of Field Crops. The experiment was carried out in open air conditions with 3 replications according to the factorial experimental design in random plots. In the trial; NC-7 peanut seeds, different biochar dose (0, 2.5, 5, 7.5, and 10 t ha-1) and different NaCl dose (0 mM, 50 mM, 100 mM and 150 mM) used. According to the results of the study; Using different doses of biochar and NaCl significantly affected the N, Ca, Mg, Cu, Mn, Zn and Fe elements in the leaves. In the trial; N content between 2.24-3.33%, Ca content between 1.15-1.75%, Mg content between 0.42-0.56%, Cu content between 1.10-6.75 mg kg-1, Mn content between 36.65-50.93 mg kg-1, Zn content 14.69-31.70 mg kg-1, Fe content varied between 272.1-565.9 mg kg-1. According to the data obtained as a result of the research, it has been observed that biochar applications can partially prevent the negative effect of excess NaCl salt on the uptake of elements, and especially the uptake of N and Fe elements increases despite salt applications.According to the results of the study; Using different doses of biochar and NaCl significantly affected the N, Ca, Mg, Cu, Mn, Zn and Fe elements in the leaves. In the trial; N content between 2.24-3.33%, Ca content between 1.15-1.75%, Mg content between 0.42-0.56%, Cu content between 1.10-6.75 mg kg-1, Mn content between 36.65-50.93 mg kg-1, Zn content 14.69-31.70 mg kg-1, Fe content varied between 272.1-565.9 mg kg-1. According to the data obtained as a result of the research, it has been observed that biochar applications can partially prevent the negative effect of excess NaCl salt on the uptake of elements, and especially the uptake of N and Fe elements increases despite salt applications.

Anahtar Kelimeler

• Peanut
• Arachis hypogaea L.
• Biochar
• NaCl
• Plant nutrients

Yerfıstığı Fideleri Üzerindeki (Arachis hypogaea L.) Tuz Stresini Azaltmada Biyokömür Materyalinin Etkileri

Öz

Tuzluluk, dünyada olduğu gibi ülkemizde de bitki gelişimini ve tarımsal verimliliği azaltan önemli sorunlardan biridir. Bitkilerin fide aşamasında çimlenme aşamasına göre tuzluluğa daha fazla duyarlı olduğu bildirilmektedir. Bunun yanında, genel olarak biyokömür kullanımının bitki büyümesi ve toprak özellikleri üzerinde çeşitli faydaları olduğu da bildirilmiştir. Bu çalışma 2021 yılında farklı oranlarda NaCI tuzunun farklı dozları ve portakal ağacından üretilen biochar materyalinin farklı dozlarının kullanıldığında NC-7 yerfıstığı çeşidinin gelişimi üzerine olan etkileri araştırılmıştır. Deneme, Bingöl Üniversitesi Ziraat Fakültesi Tarla Bitkileri arazilerinde açık hava şartlarında saksı denemesi şeklinde yapılmıştır. Araştırmada deneme deseni olarak tesadüf parsellerinde faktöriyel deneme deseni kullanılmış ve 4 tekerrürlü olarak gerçekleşmiştir. Denemede kullanılan C-7 yerfıstığı tohumları ile birlikte, farklı biochar dozlar (0, 2.5, 5, 7.5 ve 10 t ha-1) ve farklı NaCI dozları (0 mM, 50 mM, 100 mM ve 150 mM) uygulanmıştır. Tuzluluk, dünyada olduğu gibi ülkemizde de bitki gelişimini ve tarımsal verimliliği azaltan önemli sorunlardan biridir. Bitkilerin fide aşamasında çimlenme aşamasına göre tuzluluğa daha fazla duyarlı olduğu bildirilmektedir. Bunun yanında, genel olarak biochar kullanımının bitki büyümesi ve toprak özellikleri üzerinde çeşitli faydaları olduğu da bildirilmiştir. Bu çalışma 2021 yılında farklı oranlarda NaCI tuzunun farklı dozları ve portakal ağacından üretilen biochar materyalinin farklı dozlarının kullanıldığında NC-7 yerfıstığı çeşidinin gelişimi üzerine olan etkileri araştırılmıştır. Deneme, Bingöl Üniversitesi Ziraat Fakültesi Tarla Bitkileri arazilerinde açık hava şartlarında saksı denemesi şeklinde yapılmıştır. Araştırmada deneme deseni olarak tesadüf parsellerinde faktöriyel deneme deseni kullanılmış ve 4 tekerrürlü olarak gerçekleşmiştir. Denemede kullanılan NC-7 yerfıstığı tohumları ile birlikte, farklı biochar dozlar (0, 2.5, 5, 7.5 ve 10 t ha-1) ve farklı NaCI dozları (0 mM, 50 mM, 100 mM ve 150 mM) uygulanmıştır. Çalışmada elde edilen sonuçlara göre; farklı dozlarda biochar ve NaCl’nin birlikte kullanılması, bitki yapraklarındaki N, Ca, Mg, Cu, Mn, Zn ve Fe bitki besin elementlerini önemli ölçüde etkilemiştir. Denemede; bitki N içeriği %2.24-3.33arasında, bitki Ca içeriği %1.15-1.75 arasında, bitki Mg içeriği %0.42-0.56 arasında, bitki Cu içeriği 1.10-6.75 mg kg-1 arasında, bitki Mn içeriği 36.65-50.93 mg kg-1 arasında, bitki Zn içeriği 14.69-31.70 mg kg-1 arasında ve bitki Fe içeriği 272.1-565.9 mg kg-1 arasında değişim göstermiştir. Araştırma sonucunda elde edilen verilere göre, fazla miktarda NaCI tuzunun elementlerin alımındaki olumsuz etkisini biochar uygulamalarının bir miktar engelleyebildiği özellikle N ve Fe elementlerinin alımının tuz uygulamalarına rağmen arttığı gözlemlenmiştir.

Anahtar Kelimeler

• Yerfıstığı
• Arachis hypogaea L.
• Biochar
• NaCl
• Bitki besin maddeleri

Kaynakça

1. Akgul, G. (2017). Biochar: Production and application. Selcuk University Journal of Engineering Sciences, 5(4): 485-499. (In Turkish)
2. Altuner, F., Oral, E. and Baran, İ. (2022). Determination of the effects of salt (NaCl) stress on germination in some barley (Hordeum vulgare L.) varieties. Journal of Tekirdağ Agricultural Faculty, 19(1): 39-50. (In Turkish)
3. Asci, Ö. Ö., Altun, M. and Arıcı, Y. K. (2021). The effect of salt stress on some physiological properties and mineral ratios in cowpea. International Journal of Agriculture and Wildlife Science, 7(2): 297-305. (In Turkish) https://doi.org/10.24180/ijaws.921187
4. Baran, A. and Dogan, M. (2014). The effect of salicylic acid on physiological in soybean (Glycine max L.) under salt stress. Suleyman Demirel University Journal of Natural and Applied Science, 18(1):78–84. (In Turkish)
5. Beyaz, R. and Kazankaya, A. (2024). Effect of NaCl-induced salt stress on germination and initial seedling growth of Lotus corniculatus L. cv.'Leo'. Journal of Tekirdag Agricultural Faculty, 21(1): 24-34.
6. Black, C. A. (1965) Methods of Soil Analysis. American Society of Agronomy, Madison.
7. Boydak, E., Aslan, S., and Demirkiran, A. R. (2025a). Effect of different doses of orange biochar material on relieving NaCl salt stress: Peanut (Arachis hypogaea L.) applications. Turkish Journal of Agricultural and Natural Sciences, 12(1): 62-73.
8. Boydak, E., Enes, B., and Demirkiran, A. R. (2025b). Effect of leonardite material doses on the elimination of salt stress in groundnut. Türk Doğa ve Fen Dergisi, 14(1): 1-8.

9. Boydak, E., Karaaslan, D. and Turkoglu, H. (2010). The effect of different nitrogen and irrigation levels on fatty acid composition of peanut oils. Turkish Journal of Field Crops, 15(1): 29-33. https://doi.org/10.17557/tjfc.59903
10. Bulut, F. (2007). The effect of salinity on seedling growth and uptake some mineral on broad bean (Vicia faba L.). (MSc. Thesis) Marmara University, Istanbul, Türkiye. (In Turkish) https://doi.org/10.1007/978-3-540-71095-0_11031
11. Crutchfield, E. F. (2016). Biochar’s effect on plant growth and soil nutrient. (PhD Thesis) California University, U. S. A.
12. Da Silva, M., Jacqueline, J. D. F., Lúcia, H. G. C., Hugo, O. C. G., Gilvanise, A. T. and Iêde de, B. C. (2021). Chemical and physical changes of soil amended with biochar. Water, Air, and Soil Pollution, 232(8): 238. https://doi.org/10.1007/s11270-021-05289-8
13. Demirkiran, A. R. and Polat, G. D. (2025). Determination of the reaction of violet plant (Saintpaulia ionantha L.) to salinity. Journal of Tekirdag Agricultural Faculty, 22(2): 483-495.
14. Dugdug, A. A. S. (2017). Biochar for saline-sodic soil reclamation, phosphorus retention, and crop growth improvement. (PhD Thesis) University of Albert, Edmonton, Canada. https://era.library.ualberta.ca/items/6d83dad5-d268-4472-8a30-fd45cbd5c778
15. SAS (2012). JMP 10 Scripting Guide. SAS Institute. ISBN 978-1-61290-195-4.
16. Farhangi-Abriz, S. and Torabian, S. (2018). Effect of biochar on growth and ion contents of bean plant under saline condition. Environmental Science and Pollution Research, 25: 11556-11564. https://doi.org/10.1007/s11356-018-1446-z
17. Free, H. F., McGill, C. R. and Hedley, M. J. (2010). The effect of biochars on maize (Zea mays) germination. New Zealand Journal of Agricultural Research, 53(1): 1-4. https://doi.org/10.1080/00288231003606039 Gao, S. (2016). Locally produced wood biochar increases nutrient retention in agricultural soils of the San Juan Islands, WA, USA. (MSc.Thesis) University of Washington, U. S. A.
18. Ghazimahalleh, B. G., Amerian, M. R., Kahneh, E., Rahimi, M. and Tabari, Z. T. (2022). Effect of biochar, mycorrhiza, and foliar application of boron on growth and yield of peanuts. Gesunde Pflanzen, 74(4): 863-877.
19. Hussain, S., Shaukat, M., Ashraf, M., Zhu, C., Jin, Q. and Zhang, J. (2019). Salinity Stress in Arid and Semi-Arid Climates: Effects and Management in Field Crops. In: Climate Change and Agriculture, Ed(s): Hussain, S., Intechopen. https://doi.org/10.5772/intechopen.87982
20. Ibrahim, J. J., Mbega, E., Mkindi, A., Mushongi, A. and Nzilano, B. L. (2024). Appraising the effect of biochar in groundnuts (Arachis hypogaea L) growth parameters and yield under screen house conditions. International Journal of advances in Scientific Research and Engineering, 10(6): 53-64. https://doi.org/10.31695/IJASRE.2024.6.6
21. Jackson, M. L. (1962). Soil Chemical Analysis, PrenticeHall Inc., New Jersey, U. S. A.
22. Kacar, B. (1994). Plant and Soil Chemical Analysis III. Soil Analysis, Ankara University Agricultural Faculty Press no: 3, Ankara, Türkiye. (In Turkish)
23. Kacar, B. and Inal, A. (2010). Plant Analysis, Nobel Press, 912p, Ankara, Türkiye. (In Turkish)
24. Kadiroglu, A. (2018). Peanut Growht, Republic of Türkiye Ministry of Agriculture and Forestry Batı Akdeniz Agircultural Research Institute, Ankara, Türkiye. (In Turkish) https://Arastirma.Tarimorman.Gov.Tr/Batem/Belgeler/Kutuphane/TeknikBilgiler/Yerfıstığı Yetistiriciligi.Pdf30–77.
25. Kasap, Y., Demirkiran, A. R. and Serbetci, A. (1996). The effect of different level of phosphorus fertilizer on yield quality and agricultural characteristics of some groundnut varieties under the ecological conditions of Kahramanmaraş. Turkish Journal of Agriculture and Forestry, 23(supp4): 777-784. https://www.acarindex.com/pdfs/780167
26. Kaya, A. R. and Kılınc, A. (2020). Determination of yield and yield components of some peanut varieties (Arachis hypogaea L.) in Kahramanmaraş conditions. Yuzuncu Yil University Journal of the Instütute of Natural and Applied Sciences, 25(1): 21-31. (In Turkish)
27. Koldanca, E. (2016). Effect of the different sowing dates on the seed yield and quality of the peanut (Arachis hypogaea L.) under Bingöl conditions. (MSc. Thesis). Bingol University, Institute of Science, Bingöl, Türkiye. (In Turkish)
28. Liu, C., Tian, J., Chen, L., He, Q., Liu, X., Bian, R., Zheng, J., Cheng, K., Xia, S., Zhang, X., Wu, J., Li, L., Joseph, S. and Pan, G. (2024). Biochar boosted high oleic peanut production with enhanced root development and biological N fixation by diazotrophs in a sand-loamy Primisol. Science of The Total Environment, 932: 173061.
29. Mazac, R. (2016). Assessing the use of food waste biochar as a biodynamic plant fertilizer. (An Honors Thesis) Submitted for partial fulfillment of the requirements for graduation with honors in the Biology Department and Program in Environmental Studies from Hamline University, Departmental Honors Projects 43, Minnesota, U. S. A.
30. Mills, H. A. and Jones, J. B. (1996). Plant Analysis. Handbook II. Micro Macro Publishing. Inc. Georgia, U. S. A.
31. Mounirou, M. M., Kaya, E. C., Ouedraogo, A. R., Demir, K., Güneş, A. and İnal, A. (2020). Effects of biochar and organic fertilizer applications on the growth and chemical fertilizer use efficiency of onion plant. Journal of Soil Science and Plant Nutrition, 8(1): 36-45. (In Turkish)
32. Nagaraju, K., Prasad, T. N. V. K. V., Chari, M. S., Ramu, Y. R., Murthy, B. R., Naidu, M. V. S., Leelavathy, G. P., Ram Mohan, P., Damu, A. G. and Gopal, D. (2024). Effects of soil application of nanobiochar-based nitrogen and potassium fertilizers on the growth and yield of groundnut (Arachis hypogaea L.). Journal of Soil Science and Plant Nutrition, 24:5759–5771. https://doi.org/10.1007/s42729-024-01938-z
33. Ndiate, N. I., Saeed, Q., Haider, F. U., Liqun, C., Nkoh, J. N. and Mustafa, A. (2021). Co-application of biochar and arbuscular mycorrhizal fungi improves salinity tolerance, growth and lipid metabolism of maize (Zea mays L.) in an alkaline soil. Plants, 10(11): 2490. https://doi.org/10.3390/plants10112490.
34. Ndiate, N. I., Zaman, Q. U., Francis, I. N., Dada, O. A., Rehman, A., Asif, M., Goffner, D., Kane, A., Liqun, C. and Haider, F. U. (2022). Soil amendment with arbuscular mycorrhizal fungi and biochar Improves salinity tolerance, growth, and lipid metabolism of common wheat (Triticum aestivum L.). Sustainability, 14(6): 3210. https://doi.org/10.3390/su14063210
35. Olsen, S. R., Cole, C. U., Watanable, F. S. and Dean, H. C. (1954). Estimation of Available Phosphorus in Soils by Extraction with Sodium Bicarbonate. U.S. Department of Agriculture, Washington Department of Agriculture Circ. 939, Washington, U. S. A.
36. Oummu Kulthum, A. M. H. (2018). Effect of NaCl salinity on growth and development of peanut (Arachis hypogaea L.) seedlings. (MSc. Thesis). Gaziantep University, Türkiye. (In Turkish)
37. Onder, S. and Ucar, Ö. (2021). Effect of Salt Stress on Beans (Phaseolus vulgaris L.) and Applications to Increase Salt Tolerance. 3rd International Conference on Food, Agriculture and Veterinary, 19-20 June, Izmir, Türkiye.
38. Piao, J., Che, W., Li, X., Li, X., Zhang, C., Wang, Q., Jin, F. and Hua, S. (2023). Application of peanut shell biochar increases rice yield in saline-alkali paddy fields by regulating leaf ion concentrations and photosynthesis rate. Plant and Soil, 483(1): 589-606.
39. Ran, C., Gulaqa, A., Zhu, J., Wang, X., Zhang, S., Geng, Y., Guo, L., Jin, F. and Shao, X. (2020). Benefits of biochar for improving ion contents, cell membrane permeability, leaf water status and yield of rice under saline–sodic paddy field condition. Journal of. Plant Growth Regulation, 39: 370-377. https://doi.org/10.1007/s00344-019-09988-9
40. Rassem, A. and Elzobair, K. (2023). The combined effect of biochar and organic fertilizer (goat manure) on the growth and yield of peanut crop (Arachis hypogaea L.). Journal of Pure & Applied Sciences, 22(3): 172-176.
41. Ryan, J., Estefan, G. and Rashid, A. (1977). Soil and Plant Analysis Laboratory Manual, ICARDA.
42. El Sabagh, A., Omar, A., Saneoka, H. and Barutçular, C. (2016). Physiological performance of soybean germination and seedling growth under salinity stress. Dicle University Institute of Natural and Applied Science Journal, 4(1): 6–15.
43. Soothar, M. K., Mounkaila Hamani, A. K., Kumar Sootahar, M., Sun, J., Yang, G., Bhatti, S. M. and Traore, A. (2021). Assessment of acidic biochar on the growth, physiology and nutrients uptake of maize (Zea mays L.) seedlings under salinity stress. Sustainability, 13(6): 3150.
44. Sun, R., Zheng, H., Yin, S., Zhang, X., You, X., Wu, H., Suo, F., Han, K., Cheng, Y., Zhang, C. and Li, Y. (2022). Comparative study of pyrochar and hydrochar on peanut seedling growth in a coastal salt-affected soil of Yellow River Delta, China. Science of the Total Environment, 833: 155183.
45. Surucu, A., Boydak, E., Demirkıran, A. R. and Yetim, S. (2013). The effect of irrigation and nitrogen on mineral composition of peanut (Arachis hypogaea L.) leaves. Journal of Food, Agriculture and Environment, 11(3–4): 824–27.
46. Tarakcioglu, C., Özenç, D. B., Yılmaz, F. I., Kulaç, S. and Aygün, S. (2019). Effect of biochar derived from hazelnut shell on nutrient contents. Anadolu Journal of Agricultural Sciences, 34:107–117. (In Turkish) https://doi.org/10.7161/omuanajas.433030
47. Tepe, H. D. and Aydemir, T. (2017). Boron effect on growth and mineral content of lentil plant (Lens culinaris) under salt stress. Celal Bayar University Journal of Science, 13(3): 769–75. (In Turkish) https://doi.org/10.18466/cbayarfbe.339527
48. Tiryaki, İ. (2018). Adaptation mechanisms of some field plants against to salt stress. Journal of Agriculture and Nature, 21(5): 800–809. (In Turkish) https://doi.org/10.18016/ksudobil.325374
49. Turhan, A. (2020). Effect of different salinity levels on germination, seed yield, and yield characteristics in bean (Phaseolus vulgaris L.). Journal of Erciyes Agriculture and Animal Science, 3(2): 12–17. (In Turkish)
50. Wang, S., Zheng, J., Wang, Y., Yang, Q., Chen, T., Chen, Y., Chi D., Xia G., Siddique K. H. M. and Wang, T. (2021). Photosynthesis, chlorophyll fluorescence, and yield of peanut in response to biochar application. Frontiers in Plant Science, 12: 650432.
51. Xia, H., Riaz, M., Liu, B., Li, Y., El-Desouki, Z. and Jiang, C. (2022). Over two years study: Peanut biochar promoted potassium availability by mediating the relationship between bacterial community and soil properties. Applied Soil Ecology, 176: 104485.
52. Xu, C. Y., Hosseini-Bai, S., Hao, Y., Rachaputi, R. C., Wang, H., Xu, Z. and Wallace, H. (2015). Effect of biochar amendment on yield and photosynthesis of peanut on two types of soils. Environmental Science and Pollution Research, 22: 6112-6125.
53. Yao, T., Zhang, W., Gulaqa, A., Cui, Y., Zhou, Y., Weng, W., Wang, X., Liu, Q. and Jin, F. (2021). Effects of peanut shell biochar on soil nutrients, soil enzyme activity, and rice yield in heavily saline-sodic paddy field. Journal of Soil Science and Plant Nutrition, 21: 655-664.
54. Yildirim, B., Aydin, A., Terzioglu, O. and Turkozu, D. (2009). The effect of humic acid treatments on seedling development and nutrient elements gains on sugar beet (Beta vulgaris L. var. saccharifera). Türkiye VIII. Tarla Bitkileri Kongresi, 19-22 Ekim, Hatay, Türkiye. (In Turkish) https://www.researchgate.net/publication/320181426
55. Yuan, Y., Kong, Q., Zheng, Y., Zheng, H., Liu, Y., Cheng, Y., Zhang, X., Li, Z., You, X. and Li, Y. (2022). Co-application of biochar and pyroligneous acid improved peanut production and nutritional quality in a coastal soil. Environmental Technology & Innovation, 28: 102886.
56. Zambi, H. (2019). Effect of different NaCl concentrations on plant growth in some cultivars and genotypes of forage pea (Pisum sativum). (MSc. Thesis) Ordu University, Science Institute, Ordu, Türkiye. (In Turkish)
57. Zheng, J., Wang, S., Wang, R., Chen, Y., Siddique, K. H., Xia, G. and Chi, D. (2021). Ameliorative roles of biochar-based fertilizer on morpho-physiological traits, nutrient uptake and yield in peanut (Arachis hypogaea L.) under water stress. Agricultural Water Management, 257: 107129.