Piraziz Elma Çeşidinin (Türkiye) Fenolik Bileşikleri ve Biyoaktif Özellikleri

Yıl 2026, Cilt: 23 Sayı: 2, 486 - 494, 16.03.2026

Tuğba Kılıç , M. Ümit Ünal

https://doi.org/10.33462/jotaf.1625427

https://izlik.org/JA49PL29UG

Öz

Birçok hastalığın önlenmesi ve/veya tedavi edilmesi amacıyla doğal ürünlere yönelim vardır. Bu çalışmanın amacı, Türkiye'de Rosaceae familyasından yerel bir elma çeşidinin biyoaktif özelliklerini (antioksidan kapasite, antibakteriyel aktivite, enzim inhibisyonu) in vitro olarak belirlemektir. Piraziz elmasının kabuk ve eti %99.8 etanol, %99.7 metanol ve %97.0 n-hekzan çözücüleri kullanılarak ultrasonikasyon ile ekstrakte edilmiştir. Ekstrelerin fenolik bileşikleri, kantitatif olarak LC-ESI-MS/MS ile 53 standart fenolik bileşik kullanılarak belirlenmiştir. Elmanın kabuk ve su ekstreleri en yüksek toplam fenolik madde ve flavanoid içermektedir. Elma kabuk etanol ekstrelerinde 3 adet fenolik asit ve 8 adet flavonoid olmak üzere en fazla sayıda fenolik bileşik tespit edilmiştir. Ekstraktların fenolik bileşikleri arasında elma meyve etinde epikateşin, kateşin, klorojenik asit, elma kabuklarında ise epikateşin, kateşin ve izokuersetin baskındır. Klorojenik asit hariç diğer fenolik bileşikler daha fazla elma kabuk ekstrelerinde belirlenmiştir. Flavonoller (izokuersetin, astragalin, kuersetin, kaempferol) ve flavanonlar (hesperidin ve hesperetin) sadece elma kabuğu ekstraktlarında tanımlanmıştır. Kabuk ekstraktlarının α-amilaz ve α-glikozidaz inhibisyonunda meyve eti ekstraktlarından daha iyi olduğu belirlenirken, sadece kabuk ekstraktları asetilkolinesteraz (AChE) inhibisyonunda etkili olmuştur. Disk difüzyon ve ekstraktların minimum inhibitör konsantrasyonu ile belirlenen antibakteriyel aktivite değerleri mikroorganizmalara ve elma kısmının ekstraktlarına göre değişmiş ve en iyi inhibisyon zonları su ekstraktları ile elde edilmiştir. Kabuk ekstraktlarının meyve eti ekstraktlarından daha fazla biyoaktif bileşen içerdiği ve buna bağlı olarak daha iyi biyoaktif özellikler gösterdiği belirlenmiştir. Genel olarak su ekstraktları daha iyi biyolojik aktivite göstermiştir. Ekstraktların biyoaktif bileşenleri ve antioksidan kapasiteleri, enzim inhibisyonu ve antibakteriyel aktiviteleri elmanın kısmına ve çözücülere bağlıdır. Bu araştırma Piraziz elmasının özellikle kabuğunun önemli bir biyoaktif kaynak olduğunu ve farmakolojik çalışmalar için değerli olabileceğini düşündürmektedir.

Anahtar Kelimeler

Piraziz elması , Fenolikler , Enzim inhibisyonu , Asetilkolinesteraz inhibisyonu , Antibakteriyel

Etik Beyan

There is no need to obtain permission from the ethics committee for this study.

Destekleyen Kurum

Çukurova Üniversitesi Bilimsel Araştırma Projeleri

Proje Numarası

FDK-2018-10111

Phenolic Compounds and Bioactive Properties of cv. Piraziz Apple (Türkiye)

https://izlik.org/JA49PL29UG

Öz

There is a tendency towards natural products in many diseases (prevention and/or treatment). The aim was to determine the bioactive properties (antioxidant capacity, antimicrobial activity, enzyme inhibition) of a local apple variety from the Rosaceae family in Turkey in vitro. Peel and flesh of cv. Piraziz apples were extracted by ultrasonication using 99.8% ethanol, 99.7% methanol and 97.0% n-hexane solvents. Phenolic compounds of the extracts were determined quantitatively by LC-ESI-MS/MS using 53 standard compounds. Peel and water extracts contained the highest total phenolic substance and flavonoids. The highest number of phenolic compounds, 3 phenolic acids and 8 flavonoids, were detected in apple peel ethanol extracts. Among the phenolic compounds of the extracts, epicatechin, catechin, chlorogenic acid were dominant in the flesh, while epicatechin, catechin and isoquercitrin were dominant in the peels. Except for chlorogenic acid, other phenolic compounds were determined more in the peel extracts. Flavonols (isoquercitrin, astragalin, quercetin, and kaempferol) and flavanones (hesperidin and hesperetin) were identified only in apple peel extracts. While it was determined that peel extracts were better than fruit flesh extracts in inhibiting α-amylase and α-glucosidase, only peel extracts were effective in inhibiting acetylcholinesterase (AChE). Antibacterial activity values determined by disk diffusion and minimum inhibitory concentration of extracts varied according to microorganisms and apple part extracts and the best inhibition zones were obtained with water extracts. It was determined that peel extracts contained more bioactive components than fruit flesh extracts and accordingly showed better bioactive properties. In general, water extracts showed better biological activity. Bioactive components and antioxidant capacities of extracts, enzyme inhibition and antibacterial activities depend on apple parts and solvents. This study suggests that cv. Piraziz apple peel is an important bioactive source and may be valuable for pharmacological applications.

Anahtar Kelimeler

Piraziz apple , Phenolics , Enzyme inhibition , Acetylcholinesterase inhibition , Antibacterial

Etik Beyan

There is no need to obtain permission from the ethics committee for this study.

Destekleyen Kurum

Cukurova University Scientific Research Projects

Proje Numarası

FDK-2018-10111

Kaynakça

• Agourram, A., Ghirardello, D., Rantsiou, K., Zeppa, G., Belviso, S., Romane, A., Oufdou, K. and Giordano, M. (2013). Phenolic content, antioxidant potential, and antimicrobial activities of fruit and vegetable by-product extracts. International Journal of Food Properties, 16(5): 1092–1104. https://doi.org/10.1080/10942912.2011.576446
• Ağamirzaoğlu, M., Valizadeh, N. and Rahimi, A. (2024). Comparison of secondary metabolites and essential oil content of some Origanum species. Journal of Tekirdag Agricultural Faculty, 21(5): 1075–1090. https://doi.org/10.33462/jotaf.1192608
• Aidi-Wannes, W., Mhamdi, B., Sriti, J., Jemia, M. B., Ouchikh, O., Hamdaoui, G., Kchouk, M. E. and Marzouk, B. (2010). Antioxidant activities of the essential oils and methanol extracts from Myrtle (Myrtus communis var. italica L.) leaf, stem and flower. Food and Chemical Toxicology, 48: 1362–1370. https://doi.org/10.1016/j.fct.2010.03.002
• Anonim (2019). European Committee on Antimicrobial Susceptibility Testing Antimicrobial Susceptibility Testing (EUCAST), https://www.eucast.org/, (Accessed Date: 05.11.2021)
• Aoudeh, E., Şat, I. G. and Binici, H. I. (2024). Chemical properties and antioxidant activity of different extracts from purslane (Portulaca Oleracea L.). Journal of Tekirdağ Agricultural Faculty, 21(1): 81–93. https://doi.org/10.33462/jotaf.1239088
• Babbar, N., Oberoi, H. S. and Sandhu, S. K. (2013). Therapeutic and nutraceutical potential of bioactive compounds extracted from fruit residues. Critical Reviews in Food Science and Nutrition, 55(3): 319–337. https://doi.org/10.1080/10408398.2011.653734
• Béjaoui, A., Salem, I. B., Rokbeni, N., M’rabet, Y., Boussaid, M. and Boulila, A. (2017). Bioactive compounds from Hypericum humifusum and Hypericum perfoliatum: Inhibition potential of polyphenols with acetylcholinesterase and key enzymes linked to type-2 diabetes. Pharmaceutical Biology, 55(1): 906–911. https://doi.org/10.1080/13880209.2016.1270973
• Boyer, J. and Liu, R. H. (2004). Apple phytochemicals and their health benefits. Journal of Nutrition, 15: 3–5. https://doi.org/10.1186/1475-2891-3-5
• Chai, T. T., Khoo, C. S., Tee, C. S. and Wong, F. C. (2016). Alpha-glucosidase inhibitory and antioxidant potential of antidiabetic herb Alternanthera sessilis: Comparative analyses of leaf and callus solvent fractions. Pharmacognosy Magazine, 12(48): 253–258. https://doi.org/10.4103/0973-1296.192202
• Chinnici, F., Bendini, A., Gaiani, A. and Riponi, C. (2004). Radical scavenging activities of peels and pulps from cv. Golden Delicious apples as related to their phenolic composition. Journal of Agricultural and Food Chemistry, 52(15): 4684–4689. https://doi.org/10.1021/jf049770a
• Jakopic, J., Stampar, F. and Veberic, R. (2009). The ınfluence of exposure to light on the phenolic content of ‘Fuji’ apple. Scientia Horticulturae, 123: 234–239. https://doi.org/10.1016/j.scienta.2009.09.004
• Kam, A., Li, K. M., Razmovski-Naumovski, V., Nammi, S., Shi, J., Chan, K. and Li, G. Q. (2013). A comparative study on the inhibitory effects of different parts and chemical constituents of pomegranate on α-amylase and α-glucosidase. Phytotherapy Research, 27: 1614–1620. https://doi.org/10.1002/ptr.4913
• Karadeniz, T. and Aydın, H. (2015). Candidate in Organic production; Piraziz apples. II. Organic Agriculture Congress in Eastern Black Sea. 6-9 October 2015, P. 113–118, Rize, Türkiye. (In Turkish)
• Khan, S. A., Kiyumi, A. R. A., Sheidi, M. S. A. and Khusaibi, T. S. A. (2016). In vitro inhibitory effects on α-glucosidase and α-amylase level and antioxidant potential of seeds of Phoenix dactylifera L.. Asian Pacific Journal of Tropical Biomedicine, 6(4): 322–329. https://doi.org/10.1016/j.apjtb.2015.11.008
• Kumari, M. and Jain, S. (2012). Tannins: An antinutrient with positive effect to manage diabetes. Research Journal of Recent Sciences, 1(12): 1–8.
• Lima, D. S., Duarte, N. B. A., Barreto, D. L. C., de Oliveira, G. P., Takahashi, J. A., Fabrini, S. P. and Sande, D. (2018). Passion fruit and apple: from residues to antioxidant, antimicrobial and Anti-Alzheimer’s potential. Ciência Rural, 48: 09. https://doi.org/10.1590/0103-8478cr20180076
• Liu, S., Li, D., Huang, B., Chen, Y., Lu, X. and Wang, Y. (2013). Inhibition of pancreatic lipase, α-glucosidase, α-amylase, and hypolipidemic effects of the total flavonoids from Nelumbo nucifera leaves. Journal of Ethnopharmacology, 149(1): 263–269. https://doi.org/10.1016/j.jep.2013.06.034
• Mihailović, N. R., Mihailović, V. B., Kreft, S., Ćirić, A. R., Joksovic, L. G. and Đurđević, P. T. (2018). Analysis of phenolics in the peel and pulp of wild apples (Malus sylvestris (L.) Mill.). Journal of Food Composition and Analysis, 67: 1–9. https://doi.org/10.1016/j.jfca.2017.11.007
• Nkuimi-Wandjou, J. G., Lancioni, L., Barbalace, M. C., Hrelia, S., Papa, F., Sagratini, G., Vittori, S., Dall’Acqua, S., Caprioli, G., Beghelli, D., Angeloni, C., Lupidi, G. and Maggi, F. (2020). Comprehensive characterization of hytochemicals and biological activities of the Italian ancient apple ‘Mela Rosa dei Monti Sibillini’. Food Research International, 137: 109422. https://doi.org/10.1016/j.foodres.2020.109422
• Oboh, G., Ademosun, A. O., Ayeni, P. O., Omojokun O. S. and Bello, F. (2015). Comparative effect of quercetin and rutin on α-amylase, α-glucosidase and some pro-oxidant-ınduced lipid peroxidation in rat pancreas. Comparative Clinical Pathology, 24: 1103–1110. https://doi.org/10.1007/s00580-014-2040-5
• Reis, S. F., Rai, D. K. and Abu-Ghannam, N. (2012). Water at room temperature as a solvent for the extraction of apple pomace phenolic compounds. Food Chemistry, 135: 1991–1998. https://doi.org/10.1016/j.foodchem.2012.06.068
• Savatović, S. M., Ćetković, G. S., Đilas, S. M., Tumbas, V. T., Čanadanović-Brunet, J. M., Četojević-Simin, D. D. and Mandić, A. I. (2008). Antioxidant and antiproliferative activity of Granny smith apple pomace. Acta Periodica Technologica, 39: 1–212. https://doi.org/10.2298/APT0839201S
• Shahbazi, Y. (2017). Antibacteial and antioxidant properties of methanolic extracts of apple (Malus pumila), grape (Vitis vinifera), pomegranate (Punica granatum L.) and common fig (Ficus carica L.) fruits. Pharmaceutical Sciences, 23: 308–315. https://doi.org/10.15171/PS.2017.45
• Simmonds, M. S. J., and Howes, M. R. (2016). Profile of compounds in different cultivars of apple (Malus x domestica) (M.S.J., Simmonds and V.R., Preedy editor). Nutritional Composition of Fruit Cultivars, Academic Press, Chapter 1, p.1–18. https://doi.org/10.1016/B978-0-12-408117-8.00001-5
• Singleton, V. L., Orthofer, R. and Lamuela-Raventos, R. M. (1999). Analysis of total phenols and other oxidation substrates and antioxidants by means of Folin-Ciocalteu reagent. Methods in Enzymology, 299: 152–178. https://doi.org/10.1016/S0076-6879(99)99017-1
• Skoko, A. M. G., Šarkanj, B., Lores, M., Celeiro, M., Babojelić, M. S., Kamenjak, D., Flanjak, I., Jozinović, A., Kovač, T. and Lončarić, A. (2022). Identification and quantification of polyphenols in croatian traditional apple varieties. Plants, 11(24): 3540. https://doi.org/10.3390/plants11243540
• Solovchenko, A. and Schmitz-Eiberger, M. (2003). Significance of skin flavonoids for UV-B-Protection in apple fruits. Journal of Experimental Botany, 54(389): 1977–1984. https://doi.org/10.1093/jxb/erg199
• Stojiljković, D., Arsić, I and Tadić, V. (2016). Extracts of wild apple fruit (Malus sylvestris (L.) Mill., Rosaceae) as a source of antioxidant substances for use in production of nutraceuticals and cosmeceuticals. Industrial Crops and Products, 80: 165–176. https://doi.org/10.1016/j.indcrop.2015.11.023
• Sülük, K., Tosun, İ. and Ekinci, K. (2018). Determination of properties of apple processing waste and investigation of appropriate disposal methods. Bilge International Journal of Science and Technology Research, 2: 98–108. https://doi.org/10.30516/bilgesci.497147
• Thaipong, K., Boonprakob, U., Crosby, K., Cisneros-Zevallos, L. and Byrnec, L. (2006). Comparison of ABTS, DPPH, FRAP, and ORAC assays for estimating antioxidant activity from guava fruit extracts. Journal of Food Composition and Analysis, 19: 669–675. https://doi.org/10.1016/j.jfca.2006.01.003
• Uysal, S., Zengin G., Locatelli, M., Bahadori, M. B., Mocan, A., Bellagamba, G., De Luca, E., Mollica, A. and Aktumsek A. (2017). Cytotoxic and enzyme inhibitory potential of two Potentilla species (P. speciosa L. and P. reptans Willd.) and their chemical composition. Frontiers in Pharmalocogy, 8: 290. https://doi.org/10.3389/fphar.2017.00290
• Wang, H., Liu, J. and Liu, Z. (2019). Effect of enzymatic digestion, chemical and boiled water extraction techniques on apparent antioxidant bioactivities of apple peel. Journal of Food Measurement and Characterization, 13: 959–966. https://doi.org/10.1007/s11694-018-0010-3
• Wickramaratne, M. N., Punchihewa, J. C. and Wickramaratne, D. B. M. (2016). In vitro alpha amylase ınhibitory activity of the leaf extracts of Adenanthera pavonina. BMC Complementary and Alternative Medicine, 16: 466. https://doi.org/10.1186/s12906-016-1452-y
• Yabo-Dambagi, L., Akyuz, M., Aydin, T., Cakir, A. (2020). Antidiabetic and anticholinesterase properties of extracts and pure metabolites of fruit stems of Pistachio (Pistacia vera L.). Current Organic Chemistry, 24(7): 785–797. https://doi.org/10.2174/1385272824666200401111036
• Yılmaz, M. A. (2020). Simultaneous quantitative screening of 53 phytochemicals in 33 species of medicinal and aromatic plants: a detailed, robust and comprehensive LC–MS/MS method validation. Industrial Crops & Products, 149: 112347. https://doi.org/10.1016/j.indcrop.2020.112347