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Germination and Seedling Properties of Lotus corniculatus L. Under Simulated Drought Stress

Yıl 2023, Cilt: 20 Sayı: 4, 879 - 889, 25.12.2023
https://doi.org/10.33462/jotaf.1226444

Öz

Drought is an important abiotic stress factor that reduces agricultural production and yield in many crops, including forage crops, in agricultural areas around the world. Lotus corniculatus L. is the agriculturally crucial perennial legume forage crop that can tolerate moderate drought. However, studies to determine the responses of L. corniculatus to drought are limited. Therefore, this study was carried out to determine the seed germination and early seedling growth properties of L. corniculatus at different PEG6000 induced-drought treatments under in vitro conditions. In order to do this, L. corniculatus (cv. ‘AC Langille’) seeds were planted in MS (Murashige and Skoog/Gamborg) medium containing 0%, 4%, and 8% (w/v) PEG6000 for 14 days. In this study, germination percentage, mean germination time, germination rate index (speed of germination), shoot and root length, root to shoot length ratio, shoot and root fresh weight, shoot and root dry weight, shoot and root dry matter ratio, root shoot dry matter ratio, shoot and root water content and seedling vigor index parameters were measured. Our results showed that increasing drought levels resulted in an overall significant reduction in germination and seedling growth parameters except shortened mean germination time (especially, 4% PEG6000 treatment) and increased shoot and root dry matter ratio at higher (especially, 8% PEG6000 treatment) drought levels. When important growth parameters such as length, fresh and dry weight, dry matter ratio and water content, which show the development of root and shoot organs, are evaluated together, it has been determined that the root is negatively affected by drought stress at a higher rate. Based on these data, it can be concluded that the L. corniculatus will suffer a high yield loss under the drought stress at the osmotic potential (-1.03 bar) created by 8% PEG6000 treatment.

Kaynakça

  • Abdul-Baki, A. A. and Anderso, J. D. (1973). Vigor determination in soybean seed by multiple criteria. Crop Science, 13: 630-633.
  • Ahmed, M., Kheir, A. M. S., Mehmood, M. Z., Ahmad, S. and Hasanuzzaman, M. (2022). Changes in germination and seedling traits of sesame under simulated drought. Phyton, 91(4): 713-726.
  • Al-Enezi, N. A., Al-Bahrany, A. M. and Al-Khayri, J. M. (2012). Effect of X-irradiation on date palm seed germination and seedling growth. Emirates Journal of Food and Agriculture, 24(5): 415-424.
  • Aydınoğlu, E. (2019). Effects of seed treatments (priming) on germination characteristics and seedling development of certain leguminous forage crops under salt and drought stress. (MSc. Thesis) Akdeniz University, Institute of Natural and Applied Sciences.
  • Badr, A., El-Shazly, H. H., Tarawneh, R. A. and Börner, A. (2020). Screening for drought tolerance in maize (Zea mays L.) germplasm using germination and seedling traits under simulated drought conditions. Plants, 9(5): 1-23.
  • Bahrami, H., Razmjoo, J. and Jafari, A. O. (2012). Effect of drought stress on germination and seedling growth of sesame cultivars (Sesamum indicum L.). International Journal of AgriScience, 2: 423-428.
  • Bao, A. K., Wang, Y. W., Xi, J. J., Liu, C., Zhang, J. L. and Wang, S. M. (2014). Co-expression of xerophyte Zygophyllum xanthoxylum ZxNHX and ZxVP1-1 enhances salt and drought tolerance in transgenic Lotus corniculatus by increasing cations accumulation. Functional Plant Biology, 41: 203-214.
  • Beyaz, R. (2022). Morphological and biochemical changes in shoot and root organs of common vetch (Vicia sativa L.) after exposure to drought stress. Science Asia, 48: 51-56.
  • Beyaz, R., Kaya, G., Cocu, S. and Sancak C. (2011). Response of seeds and pollen of Onobrychis viciifolia and Onobrychis oxyodonta var. armena to NaCl stress. Scientia Agricola, 68(4): 477-481.
  • Bıçakçı, T., Aksu, E. ve Arslan, M. (2020). Determination of germination characteristics of covered alfalfa (Medicago sativa L.) seeds in drought stress conditions. Journal of Tekirdag Agricultural Faculty, 17(2): 124-136.
  • Bodner, G., Nakhforoosh, A. and Kaul, H. P. (2015). Management of crop water under drought: A review. Agronomy for Sustainable Development, 35(2): 401-442.
  • Bres, W., Kleiber, T., Markiewicz, B., Mieloszyk, E. and Mieloch, M. (2022). The effect of nacl stress on the response of lettuce (Lactuca sativa L.). Agronomy, (12)244:1-14.
  • Demiroğlu-Topçu, G. and Özkan, Ş. S. (2016). Effects of salt and drought stresses on germination and seedling growth of sainfoin (Onobrychis viciifolia Scop.). Journal of Agricultural Faculty of Uludag University, 30: 406-409.
  • Dissanayake, I. A. J. K., Ranwala, S. M. W. and Perera, S. S. N. (2019). Germination and seedling growth responses of Sri Lankan grown Sesame/Thala (Sesamum indicum L.) for simulated drought Conditions. Journal of the National Science Foundation of Sri Lanka, 47(4): 479-490.
  • Ellis, R. H. and Roberts, E. H. (1980). Towards a rational basis for testing seed quality. p. 605-635. In: Hebblethwaite, P.D., ed. Seed production. Butterworths, England.
  • Farooq, M., Hussain, M., Wahid, A. and Siddique, K. H. M. (2012). Drought stress in plants: an overview. Plant Responses to Drought Stress from Morphological to Molecular Features (eds R. Aroca), pp.1–33. Springer, Heidelberg, Berlin, Germany.
  • Farooq, M., Wahid, A., Kobayashi, N., Fujita, D. and Basra S. M. A. (2009). Plant drought stress: effects, mechanisms and management. In: Sustainable Agriculture (eds E. Lichtfouse, M. Navarrete, P. Debaeke, S. Véronique & C. Alberola), pp. 153–188. Springer, Dordrecht, Netherlands.
  • Gamborg, O. L., Miller, R. A. and Ojima, K. (1968). Nutrient requirements of suspension cultures of soybean root cells. Experimental Cell Research, 50: 151-155.
  • Hamidi, H. and Safarnejad, A. (2010). Effect of drought stress on alfalfa cultivars (Medicago sativa L.) in germination stage. American-Eurasian Journal of Agricultural & Environmental Sciences, 8(6): 705-709.
  • Hou, M. and Ma, M. (2022). Effect of PEG-simulated drought stress on seed germination of three medicinal liquorice (Glycyrrhiza) species. Legume Research, 45(11): 1388-1393.
  • Hunt, S. R., MacAdam, J. W. and Reeve, J. R. (2014). Establishment of birdsfoot trefoil (Lotus corniculatus) pastures on organic dairy farms in the Mountain West USA. Organic Agriculture, (1): 1-18.
  • ISTA (International Seed Testing Association). (2003). International rules for seed testing. Bassersdorf, Switzerland.
  • Jia, K., DaCosta, M. and Ebdon, J. S. (2020). Comparative effects of hydro-, hormonal-, osmotic- redox- priming on seed germination of creeping bentgrass under optimal and suboptimal temperatures. Hortscience, 55(9): 1453-1462.
  • Khan, M. N., Zhang, J., Luo, T., Liu, J., Ni, F., Rizwan, M., Fahad, S. and Hu, L. (2019). Morpho-physiological and biochemical responses of tolerant and sensitive rapeseed cultivars to drought stress during early seedling growth stage. Acta Physiologiae Plantarum, 41: 1-25.
  • Lamia, H., Naoufel, S., Larbi, K. M. and Nejib, R. M. (2012). Effect of osmotic stress on Myrtus communis germination. Biologia, 67(1): 132-136.
  • Li, H., Li, X., Zhang, D., Liu, H. and Guan, K. (2013). Effects of drought stress on the seed germınatıon and early seedlıng growth of the endemıc desert plant eremosparton songorıcum (Fabaceae). EXCLI Journal, 12: 89-101.
  • Luo, Y. Z., Li, G., Yan, G., Liu, H. and Neil, C. (2020). Turner morphological features and biomass partitioning of lucerne plants (Medicago sativa L.) subjected to water stress. Agronomy, 10(322): 1-10.
  • Maguire, J. D. (1962). Speed of germination-aid in selection and evaluation for seedling emergence and vigor. Crop Science, 2: 176-177.
  • Martínez‑Santos, E., Cruz‑Cruz, C. A., Spinoso‑Castillo, J. L. and Bello‑Bello, J. J. (2021). In vitro response of vanilla (Vanilla planifolia Jacks. ex Andrews) to PEG‑induced osmotic stress. Scientific Reports, 11(22611): 1-10.
  • Meher, Shivakrishna, P., Reddy, K. A. and Rao, D. M. (2018). Effect of PEG-6000 imposed drought stress on RNA content, relative water content (RWC), and chlorophyll content in peanut leaves and roots. Saudi Journal of Biological Sciences, 25(2018): 285-289.
  • Michel, B. E. and Kaufmann, M. R. (1973). The osmotic potential of polyethylene glycol 6000. Plant Physiology, 51: 914-916.
  • Murshed, R., Najla, S., Albiski, F., Kassem, I., Jbour, M. and Al-Said, H. (2015). Using growth parameters for in-vitro screening of potato varieties tolerant to salt stress. Journal of Agricultural Science and Technology, 17: 483-494.
  • Papadopoulos, Y. A., Choo, TM. Christie, B. R., Thomas, W. G., McKenzie, D. B., Bélanger, G., Sutherland, K., McRae, K. B. and Fillmore, S. A. E. (1998). AC Langille birdsfoot trefoil. Canadian Journal of Plant Science, 653-654.
  • Rauf, S. and Sadaqat, H. A. (2007). Effects of varied water regimes on root length, dry matter partitioning and endogenous plant growth regulators in sunflower (Helianthus annuus L.). Journal of Plant Interactions, 2(1): 41-51.
  • Snedecor, G. W. and Cochran, W. G. (1967). Statistical Methods, 6th ed. Ames, Iowa: Iowa State University Press. p 693.
  • Soltanbeigi, A. (2019). Effect of drought stress and seed pretreatment with CCC on yield and yield components of maize varieties. Journal of Tekirdag Agricultural Faculty, 16(1): 55-64.
  • Striker, G. G., Insausti, P., Grimoldi, A. A., Ploschuk, E. L. and Vasellati, V. (2005). Viviana Physiological and anatomical basis of differential tolerance to soil flooding of Lotus corniculatus L. and Lotus glaber Mill. Plant and Soil, 27: 301-311.
  • Tong, R., Liu, X., Mu, B., Wang, J., Liu, M., Zhou, Y., Qi, B., Li, Y. and Mu, C. (2021). Impact of seed maturation and drought on seed germination and early seedling growth in white clover (Trifolium repens L.). Legume Research, 44(6): 736-740.
  • Turk, M. A., Tawaha, A. R. M. and Lee, K. D. (2004). Seed germination and seedling growth of three lentil cultivars under moisture stress. Asian Journal of Plant Sciences, 3(3): 394-397.
  • Ünlüsoy, A. G., Yolcu, S., Bor, M., Özdemir, F. and Türkan, İ. (2022). Activation of Photorespiration facilitates drought stress tolerance in Lotus corniculatus. Journal of Plant Growth Regulation, 2023(42): 2088–2101.
  • Wang, W. B., Kim, Y. H., Lee, H. S., Kim, K. Y., Deng, X. P. and Kwak, S. S. (2009). Analysis of antioxidant enzyme activity during germination of alfalfa under salt and drought stresses. Plant Physiology and Biochemistry, 47: 570-577.
  • Yousefi, A. R., Rashidi, S., Moradi, P., Mastinu, A. (2020). Germination and seedling growth responses of Zygophyllum fabago, Salsola kali L. and Atriplex canescens to PEG-Induced drought stress. Environments, 7(107): 1-10.
  • Zheng, Y., Jia, A., Ning, T., Xu, J., Li, Z. and Jiang, G. (2008). Potassium nitrate application alleviates sodium chloride stress in winter wheat cultivars differing in salt tolerance. Journal of Plant Physiology, 165: 1455-1465.
  • Zhou, M. L., Ma, J. T., Zhao, Y. M., Wei, Y. H., Tang, Y. X. and Wu, Y. M. (2012). Improvement of drought and salt tolerance in Arabidopsis and Lotus corniculatus by overexpression of a novel DREB transcription factor from Populus euphratica. Gene, 506: 10-17.

Simüle Edilmiş Kuraklık Stresi Altında Lotus corniculatus L.'nin Çimlenme ve Fide Özellikleri

Yıl 2023, Cilt: 20 Sayı: 4, 879 - 889, 25.12.2023
https://doi.org/10.33462/jotaf.1226444

Öz

Kuraklık, dünyadaki tarım alanlarında yem bitkileri de dahil olmak üzere birçok üründe tarımsal üretimi ve verimi azaltan önemli bir abiyotik stres faktörüdür. Lotus corniculatus L., orta derecede kuraklığı tolere edebilen, tarımsal açıdan çok önemli bir çok yıllık baklagil yem bitkisidir. Bununla birlikte, L. corniculatus'un kuraklığa verdiği tepkileri belirlemeye yönelik çalışmalar sınırlıdır. Bu nedenle bu çalışma, L. corniculatus'un farklı PEG6000 kaynaklı kuraklık uygulamalarında in vitro koşullarda tohum çimlenmesi ve erken fide büyüme özelliklerini belirlemek amacıyla yapılmıştır. Bunu yapmak için L. corniculatus (cv. 'AC Langille') tohumları, 14 gün boyunca %0, %4 ve %8 (w/v) PEG6000 içeren MS (Murashige ve Skoog/Gamborg) ortamına ekilmiştir. Bu çalışmada çimlenme yüzdesi, ortalama çimlenme süresi, çimlenme oranı indeksi (çimlenme hızı), sürgün ve kök uzunluğu, kök sürgün uzunluğu oranı, sürgün ve kök yaş ağırlığı, sürgün ve kök kuru ağırlığı, sürgün ve kök kuru madde oranı, kök sürgün kuru madde oranı, sürgün ve kök su içeriği ve fide canlılık indeksi parametreleri ölçülmüştür. Sonuçlar, artan kuraklık seviyelerinin, kısalan ortalama çimlenme süresi (özellikle %4 PEG6000 uygulamasında) ve daha yüksek sürgün ve kök kuru madde oranı (özellikle, %8 PEG6000 uygulamasında) dışında çimlenme ve fide büyüme parametrelerinde genel olarak önemli bir azalmaya yol açtığını göstermiştir. Kök ve sürgün organlarının gelişimini gösteren uzunluk, yaş ve kuru ağırlık, kuru madde oranı ve su içeriği gibi önemli büyüme parametreleri birlikte değerlendirildiğinde, kökün kuraklık stresinden daha yüksek oranda olumsuz etkilendiği tespit edilmiştir. Bu verilere dayanarak, L. corniculatus'un %8 PEG6000 uygulaması ile oluşturulan ozmotik potansiyeldeki (-1.03 bar) kuraklık stresi altında yüksek verim kaybına uğrayacağı sonucuna varılabilir.

Kaynakça

  • Abdul-Baki, A. A. and Anderso, J. D. (1973). Vigor determination in soybean seed by multiple criteria. Crop Science, 13: 630-633.
  • Ahmed, M., Kheir, A. M. S., Mehmood, M. Z., Ahmad, S. and Hasanuzzaman, M. (2022). Changes in germination and seedling traits of sesame under simulated drought. Phyton, 91(4): 713-726.
  • Al-Enezi, N. A., Al-Bahrany, A. M. and Al-Khayri, J. M. (2012). Effect of X-irradiation on date palm seed germination and seedling growth. Emirates Journal of Food and Agriculture, 24(5): 415-424.
  • Aydınoğlu, E. (2019). Effects of seed treatments (priming) on germination characteristics and seedling development of certain leguminous forage crops under salt and drought stress. (MSc. Thesis) Akdeniz University, Institute of Natural and Applied Sciences.
  • Badr, A., El-Shazly, H. H., Tarawneh, R. A. and Börner, A. (2020). Screening for drought tolerance in maize (Zea mays L.) germplasm using germination and seedling traits under simulated drought conditions. Plants, 9(5): 1-23.
  • Bahrami, H., Razmjoo, J. and Jafari, A. O. (2012). Effect of drought stress on germination and seedling growth of sesame cultivars (Sesamum indicum L.). International Journal of AgriScience, 2: 423-428.
  • Bao, A. K., Wang, Y. W., Xi, J. J., Liu, C., Zhang, J. L. and Wang, S. M. (2014). Co-expression of xerophyte Zygophyllum xanthoxylum ZxNHX and ZxVP1-1 enhances salt and drought tolerance in transgenic Lotus corniculatus by increasing cations accumulation. Functional Plant Biology, 41: 203-214.
  • Beyaz, R. (2022). Morphological and biochemical changes in shoot and root organs of common vetch (Vicia sativa L.) after exposure to drought stress. Science Asia, 48: 51-56.
  • Beyaz, R., Kaya, G., Cocu, S. and Sancak C. (2011). Response of seeds and pollen of Onobrychis viciifolia and Onobrychis oxyodonta var. armena to NaCl stress. Scientia Agricola, 68(4): 477-481.
  • Bıçakçı, T., Aksu, E. ve Arslan, M. (2020). Determination of germination characteristics of covered alfalfa (Medicago sativa L.) seeds in drought stress conditions. Journal of Tekirdag Agricultural Faculty, 17(2): 124-136.
  • Bodner, G., Nakhforoosh, A. and Kaul, H. P. (2015). Management of crop water under drought: A review. Agronomy for Sustainable Development, 35(2): 401-442.
  • Bres, W., Kleiber, T., Markiewicz, B., Mieloszyk, E. and Mieloch, M. (2022). The effect of nacl stress on the response of lettuce (Lactuca sativa L.). Agronomy, (12)244:1-14.
  • Demiroğlu-Topçu, G. and Özkan, Ş. S. (2016). Effects of salt and drought stresses on germination and seedling growth of sainfoin (Onobrychis viciifolia Scop.). Journal of Agricultural Faculty of Uludag University, 30: 406-409.
  • Dissanayake, I. A. J. K., Ranwala, S. M. W. and Perera, S. S. N. (2019). Germination and seedling growth responses of Sri Lankan grown Sesame/Thala (Sesamum indicum L.) for simulated drought Conditions. Journal of the National Science Foundation of Sri Lanka, 47(4): 479-490.
  • Ellis, R. H. and Roberts, E. H. (1980). Towards a rational basis for testing seed quality. p. 605-635. In: Hebblethwaite, P.D., ed. Seed production. Butterworths, England.
  • Farooq, M., Hussain, M., Wahid, A. and Siddique, K. H. M. (2012). Drought stress in plants: an overview. Plant Responses to Drought Stress from Morphological to Molecular Features (eds R. Aroca), pp.1–33. Springer, Heidelberg, Berlin, Germany.
  • Farooq, M., Wahid, A., Kobayashi, N., Fujita, D. and Basra S. M. A. (2009). Plant drought stress: effects, mechanisms and management. In: Sustainable Agriculture (eds E. Lichtfouse, M. Navarrete, P. Debaeke, S. Véronique & C. Alberola), pp. 153–188. Springer, Dordrecht, Netherlands.
  • Gamborg, O. L., Miller, R. A. and Ojima, K. (1968). Nutrient requirements of suspension cultures of soybean root cells. Experimental Cell Research, 50: 151-155.
  • Hamidi, H. and Safarnejad, A. (2010). Effect of drought stress on alfalfa cultivars (Medicago sativa L.) in germination stage. American-Eurasian Journal of Agricultural & Environmental Sciences, 8(6): 705-709.
  • Hou, M. and Ma, M. (2022). Effect of PEG-simulated drought stress on seed germination of three medicinal liquorice (Glycyrrhiza) species. Legume Research, 45(11): 1388-1393.
  • Hunt, S. R., MacAdam, J. W. and Reeve, J. R. (2014). Establishment of birdsfoot trefoil (Lotus corniculatus) pastures on organic dairy farms in the Mountain West USA. Organic Agriculture, (1): 1-18.
  • ISTA (International Seed Testing Association). (2003). International rules for seed testing. Bassersdorf, Switzerland.
  • Jia, K., DaCosta, M. and Ebdon, J. S. (2020). Comparative effects of hydro-, hormonal-, osmotic- redox- priming on seed germination of creeping bentgrass under optimal and suboptimal temperatures. Hortscience, 55(9): 1453-1462.
  • Khan, M. N., Zhang, J., Luo, T., Liu, J., Ni, F., Rizwan, M., Fahad, S. and Hu, L. (2019). Morpho-physiological and biochemical responses of tolerant and sensitive rapeseed cultivars to drought stress during early seedling growth stage. Acta Physiologiae Plantarum, 41: 1-25.
  • Lamia, H., Naoufel, S., Larbi, K. M. and Nejib, R. M. (2012). Effect of osmotic stress on Myrtus communis germination. Biologia, 67(1): 132-136.
  • Li, H., Li, X., Zhang, D., Liu, H. and Guan, K. (2013). Effects of drought stress on the seed germınatıon and early seedlıng growth of the endemıc desert plant eremosparton songorıcum (Fabaceae). EXCLI Journal, 12: 89-101.
  • Luo, Y. Z., Li, G., Yan, G., Liu, H. and Neil, C. (2020). Turner morphological features and biomass partitioning of lucerne plants (Medicago sativa L.) subjected to water stress. Agronomy, 10(322): 1-10.
  • Maguire, J. D. (1962). Speed of germination-aid in selection and evaluation for seedling emergence and vigor. Crop Science, 2: 176-177.
  • Martínez‑Santos, E., Cruz‑Cruz, C. A., Spinoso‑Castillo, J. L. and Bello‑Bello, J. J. (2021). In vitro response of vanilla (Vanilla planifolia Jacks. ex Andrews) to PEG‑induced osmotic stress. Scientific Reports, 11(22611): 1-10.
  • Meher, Shivakrishna, P., Reddy, K. A. and Rao, D. M. (2018). Effect of PEG-6000 imposed drought stress on RNA content, relative water content (RWC), and chlorophyll content in peanut leaves and roots. Saudi Journal of Biological Sciences, 25(2018): 285-289.
  • Michel, B. E. and Kaufmann, M. R. (1973). The osmotic potential of polyethylene glycol 6000. Plant Physiology, 51: 914-916.
  • Murshed, R., Najla, S., Albiski, F., Kassem, I., Jbour, M. and Al-Said, H. (2015). Using growth parameters for in-vitro screening of potato varieties tolerant to salt stress. Journal of Agricultural Science and Technology, 17: 483-494.
  • Papadopoulos, Y. A., Choo, TM. Christie, B. R., Thomas, W. G., McKenzie, D. B., Bélanger, G., Sutherland, K., McRae, K. B. and Fillmore, S. A. E. (1998). AC Langille birdsfoot trefoil. Canadian Journal of Plant Science, 653-654.
  • Rauf, S. and Sadaqat, H. A. (2007). Effects of varied water regimes on root length, dry matter partitioning and endogenous plant growth regulators in sunflower (Helianthus annuus L.). Journal of Plant Interactions, 2(1): 41-51.
  • Snedecor, G. W. and Cochran, W. G. (1967). Statistical Methods, 6th ed. Ames, Iowa: Iowa State University Press. p 693.
  • Soltanbeigi, A. (2019). Effect of drought stress and seed pretreatment with CCC on yield and yield components of maize varieties. Journal of Tekirdag Agricultural Faculty, 16(1): 55-64.
  • Striker, G. G., Insausti, P., Grimoldi, A. A., Ploschuk, E. L. and Vasellati, V. (2005). Viviana Physiological and anatomical basis of differential tolerance to soil flooding of Lotus corniculatus L. and Lotus glaber Mill. Plant and Soil, 27: 301-311.
  • Tong, R., Liu, X., Mu, B., Wang, J., Liu, M., Zhou, Y., Qi, B., Li, Y. and Mu, C. (2021). Impact of seed maturation and drought on seed germination and early seedling growth in white clover (Trifolium repens L.). Legume Research, 44(6): 736-740.
  • Turk, M. A., Tawaha, A. R. M. and Lee, K. D. (2004). Seed germination and seedling growth of three lentil cultivars under moisture stress. Asian Journal of Plant Sciences, 3(3): 394-397.
  • Ünlüsoy, A. G., Yolcu, S., Bor, M., Özdemir, F. and Türkan, İ. (2022). Activation of Photorespiration facilitates drought stress tolerance in Lotus corniculatus. Journal of Plant Growth Regulation, 2023(42): 2088–2101.
  • Wang, W. B., Kim, Y. H., Lee, H. S., Kim, K. Y., Deng, X. P. and Kwak, S. S. (2009). Analysis of antioxidant enzyme activity during germination of alfalfa under salt and drought stresses. Plant Physiology and Biochemistry, 47: 570-577.
  • Yousefi, A. R., Rashidi, S., Moradi, P., Mastinu, A. (2020). Germination and seedling growth responses of Zygophyllum fabago, Salsola kali L. and Atriplex canescens to PEG-Induced drought stress. Environments, 7(107): 1-10.
  • Zheng, Y., Jia, A., Ning, T., Xu, J., Li, Z. and Jiang, G. (2008). Potassium nitrate application alleviates sodium chloride stress in winter wheat cultivars differing in salt tolerance. Journal of Plant Physiology, 165: 1455-1465.
  • Zhou, M. L., Ma, J. T., Zhao, Y. M., Wei, Y. H., Tang, Y. X. and Wu, Y. M. (2012). Improvement of drought and salt tolerance in Arabidopsis and Lotus corniculatus by overexpression of a novel DREB transcription factor from Populus euphratica. Gene, 506: 10-17.
Toplam 44 adet kaynakça vardır.

Ayrıntılar

Birincil Dil İngilizce
Konular Toprak Bilimleri ve Bitki Besleme (Diğer)
Bölüm Makaleler
Yazarlar

Ramazan Beyaz 0000-0003-4588-579X

Erken Görünüm Tarihi 15 Aralık 2023
Yayımlanma Tarihi 25 Aralık 2023
Gönderilme Tarihi 29 Aralık 2022
Kabul Tarihi 2 Nisan 2023
Yayımlandığı Sayı Yıl 2023 Cilt: 20 Sayı: 4

Kaynak Göster

APA Beyaz, R. (2023). Germination and Seedling Properties of Lotus corniculatus L. Under Simulated Drought Stress. Tekirdağ Ziraat Fakültesi Dergisi, 20(4), 879-889. https://doi.org/10.33462/jotaf.1226444
AMA Beyaz R. Germination and Seedling Properties of Lotus corniculatus L. Under Simulated Drought Stress. JOTAF. Aralık 2023;20(4):879-889. doi:10.33462/jotaf.1226444
Chicago Beyaz, Ramazan. “Germination and Seedling Properties of Lotus Corniculatus L. Under Simulated Drought Stress”. Tekirdağ Ziraat Fakültesi Dergisi 20, sy. 4 (Aralık 2023): 879-89. https://doi.org/10.33462/jotaf.1226444.
EndNote Beyaz R (01 Aralık 2023) Germination and Seedling Properties of Lotus corniculatus L. Under Simulated Drought Stress. Tekirdağ Ziraat Fakültesi Dergisi 20 4 879–889.
IEEE R. Beyaz, “Germination and Seedling Properties of Lotus corniculatus L. Under Simulated Drought Stress”, JOTAF, c. 20, sy. 4, ss. 879–889, 2023, doi: 10.33462/jotaf.1226444.
ISNAD Beyaz, Ramazan. “Germination and Seedling Properties of Lotus Corniculatus L. Under Simulated Drought Stress”. Tekirdağ Ziraat Fakültesi Dergisi 20/4 (Aralık 2023), 879-889. https://doi.org/10.33462/jotaf.1226444.
JAMA Beyaz R. Germination and Seedling Properties of Lotus corniculatus L. Under Simulated Drought Stress. JOTAF. 2023;20:879–889.
MLA Beyaz, Ramazan. “Germination and Seedling Properties of Lotus Corniculatus L. Under Simulated Drought Stress”. Tekirdağ Ziraat Fakültesi Dergisi, c. 20, sy. 4, 2023, ss. 879-8, doi:10.33462/jotaf.1226444.
Vancouver Beyaz R. Germination and Seedling Properties of Lotus corniculatus L. Under Simulated Drought Stress. JOTAF. 2023;20(4):879-8.