Determination of Biological Activity of Some Macro/Micro Algae

Öz

Algae are primary producers in the aquatic environment and from an important part of the food chain. Algae have secondary metabolites with high biological activity, which are especially important in the creation of novel pharmaceutical agents. Main purpose of the research was to ascertain antioxidant, anti-alzheimer, anti-diabetic and tyrosinase inhibitory activities of ethanol, hexane and water extracts of some macro/micro algae. Maceration was used as the sample prepatation method. Antioxidant activity of algea for different radical and compound was assessed. Anti-Alzheimer activity of the obtained calluses were determined using Ellman method which is a spectrophotometric method. Anti-diabetic activities were determined using the α-glucosidase inhibition method. It was discovered that the water extract contained more antioxidant phytochemicals, including phenolic (587.54±2.12 mg PEs/g DW) and flavonoids (618.39±2.47 mg QEs/g DW). This study confirmed that the water extract contained high levels of biological acitivty. This extract may be utilized as a possible source of beneficial nutrients or antioxidants.

Anahtar Kelimeler

• Macro/micro algae
• antioxidant activity
• anti-alzheimer activity
• anti-diabetic activity

Kaynakça

1. Chapman, R.L., Buchheim, M.A. (2001). Green algae and the evolution of land plants: inferences from nuclear-encoded rRNA gene sequences. Biosystems. 28(1-3): 127-137.
2. Barbier, P., Guise, S., Huitorel, P., Amade, P., Pesando, D., Briand, C., Peyrot, V. (1992). Caulerpenyne from Caulerpa taxifolia has an antiproliferative activity on tumor cell line SK-N-SH and modifies the microtubule network. Life science. 70(4): 415-429
3. Belton, G.S., Draisma, S., Van Reine, W.F.P., Huisman, J.M., Gurgel, C.F.D. (2019). A taxonomic reassessment of Caulerpa (Chlorophyta, Caulerpaceae) in southern Australia, based on tufA and rbcL sequence data. Pyhcologica. 58(3): 234-253
4. Ceylan B., Yeşiloğlu, Y. (2022). Investigation of antioxidant activity of Sorbus domestica L. extracts and determination of phenolic contents by LC-MS/MS. Revue Roumaine de Chimie. 67(6-7): 343-351
5. Ertaş, A., Gören, A.C., Boğa, M., Yeşil, Y., Kolak, U. (2014). Essential oil compositions and anticholinesterase activities of two edible plants Tragopogon latifolius var. angustifolius and Lycopsis orientalis. Natural Product Research. 28(17): 1405-1408
6. Thilagam, E., Parimaladevi, B., Kumarappan, C., Mandal, S.C. (2013). α-glucosidase and α-amylase inhibitory activity of Senna surattensis. Journal of Acupuncture and Meridian Studies. 6(1): 24-30
7. Moreno, M.I.N., Isla, M.I., Sampietro, A.R., Vattuone, M.A. (2000). Comparasion of the free radical-scavenging activity of propolis from several regions of Argentina. Journal of Ethnopharmacology. 71(1-2): 109-114
8. Slinkard, K., Singleton V. (1977). Total Phenol Analysis: Automation and Comparasion with Manual Methods. American Journal of Enology and Viticulture. 28: 49-55.

9. Ellman, G.L., Courtney, K.D., Andress, V.J., Feather-Stone, R.M. (1961). A new rapid colorimetric determination of acetylcholinesterase activity. Biochemistry Pharmacology. 7: 88-95
10. Tsujii, E., Muroi, M., Shiragami, N., Takatsuki, A. (1996). Nectrisine is a potent inhibitor of alpha-glucosidases, demonstrating activities similarly at enzyme and cellular levels. Biochemical and Biophysical Research Communications. 220(2): 459-466
11. Keramane, B., Sanchez-Camargo, A.P., Montero, L., Laincer, F., Bedjou, F., Ibanez, E. (2023). Pressurized liquid extraction of bioactive extracts with antioxidant and antibacterial activity from green, red and Brown Algerian algae. Algal Research 76: 103293.12. Ellman GL, Courtney KD, Andress VJ, Feather-Stone RM. A new and rapid colorimetric determination of acetylcholinesterase activity, Biochem. Pharmacology. 7: 88-95
12. Zaid, A. A. A., Hammad, D. M., & Sharaf, E. M. (2015). Antioxidant and anticancer activity of Spirulina platensis water extracts, 11(7): 846-851.
13. Trigui M., Gasmi L., Zouari I., Tounsi S. (2013). Seasonal variation in phenolic composition, antibacterial and antioxidant activities of Ulva rigida (Chlorophyta) and assessment of antiacetylcholinesterase potential. J. Appl. Phycol. 25: 319-328.