Comparative Antioxidant Capacity and Enzyme Inhibitory Effect of Extracts from Prunus avium Leaves

Yıl 2020, Cilt: 20 Sayı: 3, 234 - 242, 30.12.2020

Sengul Uysal

https://doi.org/10.17475/kastorman.849538

Cited By: 1

Öz

Aim of study: This study aimed to investigate the antioxidant capacity and enzyme inhibitory effect of different solvent extracts obtained from Prunus avium leaves.
Material and methods: The antioxidant capacity were evaluated using in vitro assays including DPPH, ABTS, CUPRAC, FRAP, phosphomolybdenum and metal chelating activity. Enzyme inhibitory effects were screened against AChE, BChE, α-amylase, α-glucosidase and tyrosinase enzyme. Additionally, total phenolic and flavonoid content were determined spectrophotometrically.
Main results: The water extract yielded the highest phenolic content (66.65 mgGAE/g). In antioxidant assays, water extract exhibited strong antioxidant capacity (with exception of metal chelating activity). Methanol extract showed the highest enzyme inhibitory effect compared to other extracts.
Highlights: This study proved that P. avium leaves have the potential for the development of new phytopharmaceuticals or food additives.

Anahtar Kelimeler

Prunus avium, Antioxidant, Enzyme Inhibition

Prunus avium Yapraklarından Elde Edilen Ekstraktların Karşılaştırılmalı Antioksidan Kapasitesi ve Enzim Inhibisyon Etkisi

Öz

Çalışmanın amacı: Bu çalışmanın amacı Prunus avium yapraklarından elde edilen farklı çözücü ekstraktlarının antioksidan kapasitesini ve enzim inhibitör aktivitesini araştırmaktır.
Materyal ve yöntem: Antioksidan kapasite DPPH, ABTS, CUPRAC, FRAP, fosfomolibdat ve metal şelatlamayı içeren in vitro deneylerle değerlendirildi. Enzim inhibitor ektisi AChE, BChE, α-amilaz, α-glukozidaz ve tirozinaz enzimlerine karşı tarandı. Ek olarak, total fenolik ve flavonoid içerik spektrofotometrik olarak belirlendi.
Temel sonuçlar: Su ekstraktı en yüksek fenolik içeriğe sahiptir (66.65 mgGAE/g). Antioksidan deneylerde, su ekstraktı güçlü antioksidan kapasitesi sergiledi (metal şelatlama aktivitesi istisna olarak). Metanol ekstraktı diğer ekstraktlarla karşılaştırıldığında en yüksek enzim inhibitor etki gösterdi.
Araştırma vurguları: Bu çalışma P. avium yapraklarının yeni fitofarmasötikler veya gıda katkı maddelerinin geliştirme potansiyeline sahip olduğunu kanıtladı

Anahtar Kelimeler

Prunus avium, Antioksidan, Enzim İnhibisyonu

Kaynakça

• Acero, N., Gradillas, A., Beltran, M., García, A. & Mingarro, D.M. (2019). Comparison of phenolic compounds profile and antioxidant properties of different sweet cherry (Prunus avium L.) varieties. Food Chemistry, 279, 260-271.
• Asghari, B., Mafakheri, S., Zarrabi, M.M., Erdem, S. A., Orhan, I.E., & Bahadori, M.B. (2019). Therapeutic target enzymes inhibitory potential, antioxidant activity, and rosmarinic acid content of Echium amoenum. South African Journal of Botany, 120, 191-197.
• Azwanida, N. N. (2015). A review on the extraction methods use in medicinal plants, principle, strength and limitation. Medicinal & Aromatic Plants, 4(196), 2167-0412.
• Bahadori, M.B., Zengin, G., Bahadori, S., Dinparast, L., & Movahhedin, N. (2018). Phenolic composition and functional properties of wild mint (Mentha longifolia var. calliantha (Stapf) Briq.). International Journal of Food Properties, 21(1), 183-193.
• Banerjee, J., Singh, R., Vijayaraghavan, R., MacFarlane, D., Patti, A.F. & Arora, A. (2017). Bioactives from fruit processing wastes: Green approaches to valuable chemicals. Food Chemistry, 225, 10-22.
• Chai, W.M., Huang, Q., Lin, M.Z., Ou-Yang, C., Huang, W.Y., Wang, Y.X., Xu, K.L. & Feng, H.L. (2018). Condensed tannins from longan bark as inhibitor of tyrosinase: structure, activity, and mechanism. Journal of Agricultural and Food Chemistry, 66(4), 908-917.
• Dziadek, K., Kopeć, A. & Tabaszewska, M. (2019). Potential of sweet cherry (Prunus avium L.) by-products: Bioactive compounds and antioxidant activity of leaves and petioles. European Food Research and Technology, 245, 763–772.
• Dziadek, K., Kopeć, A. & Czaplicki, S. (2018). The petioles and leaves of sweet cherry (Prunus avium L.) as a potential source of natural bioactive compounds. European Food Research and Technology, 244(8), 1415–1426.
• Gonçalves, B., Landbo, A.K., Knudsen, D., Silva, A.P., Moutinho-Pereira, J., Rosa, E. & Meyer, A.S. (2004). Effect of ripeness and postharvest storage on the phenolic profiles of cherries (Prunus avium L.). Journal of Agricultural and Food Chemistry, 52(3), 523-530.
• Grochowski, D.M., Uysal, S., Zengin, G. & Tomczyk, M. (2019). In vitro antioxidant and enzyme inhibitory properties of Rubus caesius L. International Journal of Environmental Health Research, 29(3), 237-245.
• Jesus, F., Gonçalves, A.C., Alves, G. & Silva, L.R. (2019). Exploring the phenolic profile, antioxidant, antidiabetic and anti-hemolytic potential of Prunus avium vegetal parts. Food Research International, 116, 600-610.
• Kelebek, H. & Selli, S. (2011). Evaluation of chemical constituents and antioxidant activity of sweet cherry (Prunus avium L.) cultivars. International Journal of Food Science & Technology, 46(12), 2530-2537.
• Kent, K., Charlton, K.E., Jenner, A. & Roodenrys, S. (2016). Acute reduction in blood pressure following consumption of anthocyanin-rich cherry juice may be dose-interval dependant: a pilot cross-over study. International Journal of Food Sciences and Nutrition, 67(1), 47-52.
• Li, H. Y., Hao, Z. B., Wang, X. L., Huang, L. & Li, J.P. (2009). Antioxidant activities of extracts and fractions from Lysimachia foenum-graecum Hance. Bioresource Technology, 100(2), 970-974.
• Masondo, N. A., Stafford, G. I., Aremu, A.O. & Makunga, N.P. (2019). Acetylcholinesterase inhibitors from southern African plants: An overview of ethnobotanical, pharmacological potential and phytochemical research including and beyond Alzheimer's disease treatment. South African Journal of Botany, 120, 39-64.
• McCune, L.M., Kubota, C., Stendell-Hollis, N.R. & Thomson, C.A. (2010). Cherries and health: a review. Critical Reviews in Food Science and Nutrition, 51(1), 1-12.
• Mollica, A., Zengin, G., Durdagi, S., Ekhteiari Salmas, R., Macedonio, G., Stefanucci, A., Dimmito, M.P. & Novellino, E. (2019). Combinatorial peptide library screening for discovery of diverse α-glucosidase inhibitors using molecular dynamics simulations and binary QSAR models. Journal of Biomolecular Structure and Dynamics, 37(3), 726-740.
• Mollica, A., Zengin, G., Stefanucci, A., Ferrante, C., Menghini, L., Orlando, G., Brunetti, L., Locatelli, M., Dimmito, M.P., Novellino, E., Wakeel, O.K., Ogundeji, M.O., Onaolapo, A.Y. & Onaolapo, O.J. (2018). Nutraceutical potential of Corylus avellana daily supplements for obesity and related dysmetabolism. Journal of Functional Foods, 47, 562-574.
• Murayyan, A.I., Manohar, C.M., Hayward, G. & Neethirajan, S. (2017). Antiproliferative activity of Ontario grown onions against colorectal adenocarcinoma cells. Food Research International, 96, 12-18.
• Nawirska-Olszańska, A., Kolniak-Ostek, J., Oziembłowski, M., Ticha, A., Hyšpler, R., Zadak, Z., Zidova, P. & Paprstein, F. (2017). Comparison of old cherry cultivars grown in Czech Republic by chemical composition and bioactive compounds. Food Chemistry, 228(1), 136-142.
• Rauf, A. & Jehan, N. (2017). Natural products as a potential enzyme inhibitors from medicinal plants. In Enzyme Inhibitors and Activators, 165-177.
• Scalbert, A., Johnson, I.T. & Saltmarsh, M. (2005). Polyphenols: antioxidants and beyond. The American Journal of Clinical Nutrition, 81(1), 215-217.
• Serradilla, M.J., Martín, A., Ruiz-Moyano, S., Hernández, A., López-Corrales, M. & de Guía Córdoba, M. (2012). Physicochemical and sensorial characterisation of four sweet cherry cultivars grown in Jerte Valley (Spain). Food Chemistry, 133(4), 1551-1559.
• Średnicka-Tober, D., Ponder, A., Hallmann, E., Głowacka, A. & Rozpara, E. (2019). The profile and content of polyphenols and carotenoids in local and commercial sweet cherry fruits (Prunus avium L.) and their antioxidant activity in vitro. Antioxidants, 8(11), 534.
• Usenik, V., Fabčič, J. & Štampar, F. (2008). Sugars, organic acids, phenolic composition and antioxidant activity of sweet cherry (Prunus avium L.). Food Chemistry, 107(1), 185-192.
• Uysal, A., Ozer, O.Y., Zengin, G., Stefanucci, A., Mollica, A., Picot-Allain, C. M. N. & Mahomoodally, M. F. (2019). Multifunctional approaches to provide potential pharmacophores for the pharmacy shelf: Heracleum sphondylium L. subsp. ternatum (Velen.) Brummitt. Computational Biology and Chemistry, 78, 64-73.
• Uysal, S., Zengin, G., Locatelli, M., Bahadori, M. B., Mocan, A., Bellagamba, G., De Luca, E., Mollica, A. & Aktumsek, A. (2017). Cytotoxic and enzyme inhibitory potential of two Potentilla species (P. speciosa L. and P. reptans Willd.) and their chemical composition. Frontiers in Pharmacology, 8, 290.
• Uysal, S. & Aktumsek, A. (2015). A phytochemical study on Potentilla anatolica: an endemic Turkish plant. Industrial Crops and Products, 76, 1001-1007.
• Wu, T., Tang, Q., Yu, Z., Gao, Z., Hu, H., Chen, W., Zheng, X. & Yu, T. (2014). Inhibitory effects of sweet cherry anthocyanins on the obesity development in C57BL/6 mice. International Journal of Food Sciences and Nutrition, 65(3), 351-359.
• Zengin, G., Stefanucci, A., Rodrigues, M.J., Mollica, A., Custodio, L., Aumeeruddy, M.Z., & Mahomoodally, M. F. (2019). Scrophularia lucida L. as a valuable source of bioactive compounds for pharmaceutical applications: in vitro antioxidant, anti-inflammatory, enzyme inhibitory properties, in silico studies, and HPLC profiles. Journal of Pharmaceutical and Biomedical Analysis, 162, 225-233.