Comparative Antioxidant Capacity and Enzyme Inhibitory Effect of Extracts from Prunus avium Leaves

Year 2020, Volume: 20 Issue: 3, 234 - 242, 30.12.2020

Sengul Uysal

https://doi.org/10.17475/kastorman.849538

Cited By: 2

Abstract

Aim of study: This study aimed to investigate the antioxidant capacity and enzyme inhibitory effect of different solvent extracts obtained from Prunus avium leaves.
Material and methods: The antioxidant capacity were evaluated using in vitro assays including DPPH, ABTS, CUPRAC, FRAP, phosphomolybdenum and metal chelating activity. Enzyme inhibitory effects were screened against AChE, BChE, α-amylase, α-glucosidase and tyrosinase enzyme. Additionally, total phenolic and flavonoid content were determined spectrophotometrically.
Main results: The water extract yielded the highest phenolic content (66.65 mgGAE/g). In antioxidant assays, water extract exhibited strong antioxidant capacity (with exception of metal chelating activity). Methanol extract showed the highest enzyme inhibitory effect compared to other extracts.
Highlights: This study proved that P. avium leaves have the potential for the development of new phytopharmaceuticals or food additives.

Keywords

Prunus avium, Antioxidant, Enzyme Inhibition

Prunus avium Yapraklarından Elde Edilen Ekstraktların Karşılaştırılmalı Antioksidan Kapasitesi ve Enzim Inhibisyon Etkisi

Abstract

Çalışmanın amacı: Bu çalışmanın amacı Prunus avium yapraklarından elde edilen farklı çözücü ekstraktlarının antioksidan kapasitesini ve enzim inhibitör aktivitesini araştırmaktır.
Materyal ve yöntem: Antioksidan kapasite DPPH, ABTS, CUPRAC, FRAP, fosfomolibdat ve metal şelatlamayı içeren in vitro deneylerle değerlendirildi. Enzim inhibitor ektisi AChE, BChE, α-amilaz, α-glukozidaz ve tirozinaz enzimlerine karşı tarandı. Ek olarak, total fenolik ve flavonoid içerik spektrofotometrik olarak belirlendi.
Temel sonuçlar: Su ekstraktı en yüksek fenolik içeriğe sahiptir (66.65 mgGAE/g). Antioksidan deneylerde, su ekstraktı güçlü antioksidan kapasitesi sergiledi (metal şelatlama aktivitesi istisna olarak). Metanol ekstraktı diğer ekstraktlarla karşılaştırıldığında en yüksek enzim inhibitor etki gösterdi.
Araştırma vurguları: Bu çalışma P. avium yapraklarının yeni fitofarmasötikler veya gıda katkı maddelerinin geliştirme potansiyeline sahip olduğunu kanıtladı

Keywords

Prunus avium, Antioksidan, Enzim İnhibisyonu

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