Ozonlanmış Zeytinyağı ve Ozonlanmış Distile Suyun Sağlıklı Kolon Fibroblast Hücreleri ve Kolon Kanseri Hücreleri Üzerindeki Proliferatif Etkisinin İncelenmesi

Yıl 2024, Cilt: 14 Sayı: 1, 224 - 233, 15.03.2024

Ece Avuloğlu Yılmaz , Şenol Toprak , Aybüke Afra Babacan

https://doi.org/10.31466/kfbd.1388212

Öz

Ozon terapi, hastalıkların ve farklı tıbbi durumların tedavisinde ozon gazının vücuda verilmesine dayanan alternatif bir tedavi şeklidir ve bazı çalışmalarda ozon uygulamaları önerilmektedir. Bu çalışmada, saf zeytinyağı ve distile suyun ozon gazı ile zenginleştirilerek olası anti-kanser aktivitesinin belirlenmesi ve kolon kanseri ve normal kolon fibroblast hücreleri üzerindeki sitotoksik etkisinin saptanması amaçlanmıştır. Ozonla zenginleştirilmiş saf zeytinyağı ve distile suyun hücre canlılığı üzerindeki etkisi DLD1 (kolon kanseri) ve CCD-18Co (sağlıklı kolon fibroblastı) hücre hatları üzerinde MTT testi ile belirlenmiştir. DLD-1 hücre hattında, ozonlanmış distile su ve zeytinyağı tüm konsantrasyonlarda in vitro hücre canlılığını azaltmıştır ve bu azalma en yüksek konsantrasyonlarda (5 ve 10 ppm) daha belirgindir. CCD-18Co hücre hattında, ozonlanmış distile su ve ozonlanmış zeytinyağı tüm konsantrasyonlarda in vitro hücre canlılığını artırmıştır, ancak bu artış kontrole kıyasla anlamlı değildir. Bu çalışmanın sonuçları literatürdeki diğer çalışmaların sonuçlarıyla uyumludur. Bu nedenle, ozon tedavisinin kanser tedavisinde umut verici olduğu düşünülmektedir.

Anahtar Kelimeler

Ozon, MTT testi, Kolon kanseri, DLD1, CCD-18Co

Proje Numarası

This study was not supported by any funder.

Examining the Proliferative Effect of Ozonated Olive Oil and Ozonated Distilled Water on Healthy Colon Fibroblast Cells and Colon Cancer Cells

Öz

Ozone therapy is an alternative form of treatment based on the administration of ozone gas to the body in the treatment of diseases and different medical conditions, and ozone applications are recommended in some studies. In this study, it was aimed to determine the possible anti-cancer activity by enriching extra virgin olive oil and distilled water with ozone gas and determining its cytotoxic effect on colon cancer and normal colon fibroblast cells. The effect of ozone enriched extra virgin olive oil and distilled water on cell viability was determined by MTT assay on DLD1 (colon cancer) and CCD-18Co (healthy colon fibroblast) cell lines. In the DLD-1 cell line, ozonated distilled water and olive oil decreased in vitro cell viability at all concentrations and this decrease was most pronounced at higher concentrations (5 and 10 ppm). In the CCD-18Co cell line, ozonized distilled water and ozonized olive oil increased in vitro cell viability at all concentrations, but this increase was not significant compared to the control. The results of this study are consistent with the results of other studies in the literature. Therefore, ozone therapy is thought to be promising in cancer treatment.

Anahtar Kelimeler

Ozone, MTT assay, Colon cancer, DLD1, CCD-18Co

Etik Beyan

The author declares that this study complies with Research and Publication Ethics. Ethical approval is not required for this research article.

Destekleyen Kurum

This study was not supported by any funder.

Proje Numarası

This study was not supported by any funder.

Teşekkür

Author wishes to thank Dr. Ceren BAŞKAN for her support.

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