KEÇİBOYNUZUNUN (CERATONİA SİLİQUA L.) NİKOTİN BAZLI OKSİDATİF STRESE ETKİSİ

Öz

AMAÇ: Bu çalışmada keçiboynuzu bitkisinin antioksidan etkisi, nikotin kaynaklı oksidatif strese ve rat hipokampüsü cornu ammonisinde (CA) piramidal hücre sayısına etkisi araştırılmıştır. GEREÇ VE YÖNTEM: Çalışmada 28 adet yetişkin Wistar Albino erkek rat kullanıldı. Ratlar kontrol grubu, keçiboynuzu grubu, nikotin grubu ve nikotin+keçiboynuzu grubu olmak üzere dört gruba ayrıldı. Deney grubuna nikotin, tedavi grubuna ise nikotin ile birlikte keçiboynuzu ekstresi uygulandı. Akciğer, beyin, böbrek, kalp ve karaciğer dokularında superoksit dismutaz (SOD), glutatyon (GSH), glutatyon disülfit (GSSG), total oksidan seviye (TOS), total antioksidan seviye (TAS) değerleri spektrofotometrik analiz ile ölçüldü. Oksidatif stres indeksi (OSI) ve GSH/GSSG değerleri sırasıyla TOS/TAS ve GSH/GSSG oranları şeklinde hesaplandı. CA'daki piramidal hücre sayısı, optik fraksiyonlama tekniği kullanılarak belirlendi. BULGULAR: Nikotin grubunun böbrek dokusundaki TAS düzeyinin kontrol ve keçiboynuzu gruplarına göre anlamlı derecede düşük olduğu görüldü. Beyin dokusunda, nikotin grubunun TAS düzeyi diğer gruplara göre önemli ölçüde düşüktü (p<0.001). Nikotin grubunun OSI değeri karaciğer dokusunda kontrol grubuna göre anlamlı derecede yüksekti (p<0.001). Nikotine maruz kalmanın CA'daki piramidal hücre sayısında önemli bir azalmaya neden olduğu gösterildi. SONUÇ: Keçiboynuzu bitkisinin oksidatif stres oluşumunda TAS düzeyini artırarak nikotine karşı antioksidan özelliklere sahip önemli bir fitomedikal bitki olabileceği kanısına varılmıştır.

Anahtar Kelimeler

• Keçiboynuzu özü
• Nikotin
• Oksidatif stres
• Piramidal hücreler

THE EFFECT OF CAROB (CERATONIA SILIQUA L.) AGAINST NICOTINE BASED OXIDATIVE STRESS

Öz

OBJECTIVE: In this study the antioxidant effect of carob plant, ıt's effects of nicotine-induced oxidative stress and the number of pyramidal cells in the rat hippocampus in cornu ammonis (CA) were investigated. MATERIAL AND METHODS: In the study, 28 adult Wistar Albino male rats were used. Rats are divided into four groups as control group, carob group, nicotine group and nicotine + carob group. Nicotine was applied to the experimental group, and carob extract was applied to the treatment group as well as nicotine. Superoxide dismutase (SOD), glutathione (GSH), glutathione disulfide (GSSG), total oxidant capacity (TOS), total antioxidant capacity (TAS) values were measured by spectrophotometric analysis on the lung, brain, kidney, heart and liver tissues. Oxidative stress index (OSI) and GSH / GSSG values, respectively, were calculated as TOS/TAS and GSH/GSSG rates. The number of pyramidal cells in the CA was estimated using the optical fractionator technique. RESULTS: It is seen that the TAS level in the kidney tissue of the nicotine group is significantly lower than the control and carob groups. In brain tissue, the TAS level of the nicotine group was significantly lower than that of other groups (p<0.001). The OSI value of the nicotine group was significantly higher in liver tissue compared to the control group (p<0.001). Nicotine exposure has been shown to cause a significant reduction in the number of pyramidal cells in CA. CONCLUSIONS: It is understood that Carob plant is an important phytomedical plant that has antioxidant properties against nicotine by increasing TAS level in oxidative stress formation.

Anahtar Kelimeler

• Carob extract
• Nicotine
• Oxidative stress
• Pyramidal cells.

Kaynakça

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