Araştırma Makalesi

Extraction of Polyphenol Oxidase Enzyme of Black Mulberry (Morus nigra L.)

Cilt: 2026 Sayı: 17 1 Ekim 2026
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Extraction of Polyphenol Oxidase Enzyme of Black Mulberry (Morus nigra L.)

Öz

This study focuses on developing a high-efficiency, cost-effective three-phase partitioning (TPP) purification strategy for polyphenol oxidase (PPO) from black mulberry (Morus nigra) fruit, known for its rich antioxidant profile. Reaction conditions were optimized, and maximum PPO activity was achieved in a system containing 100 mM sodium phosphate buffer (pH 7.0), 10 mM ascorbic acid, 0.5% (w/v) PEG 6000, and 50 mM K₂HPO₄. Under optimized TPP conditions, PPO was purified with an exceptionally high activity recovery of 177% and a 2.7-fold increase in purity, demonstrating the efficiency of this approach compared with conventional purification strategies. The methodological simplicity, scalability, and environmentally friendly nature of the process highlight its potential as a practical alternative for enzyme purification from natural sources. Overall, this study provides a cost-effective, efficient protocol for PPO recovery, offering significant industrial relevance for food biotechnology.

Anahtar Kelimeler

Destekleyen Kurum

Kocaeli University

Proje Numarası

FYL-2022-3190

Etik Beyan

The author(s) of this article declare that the materials and methods used in this study do not require ethical committee permission and/or legal-special permission.

Teşekkür

The authors would like to thank Kocaeli University (Project No: FYL-2022-3190) for funding.

Kaynakça

  1. [1] Özcan O, Erdal H, Çakırca G, Yönden Z. Oksidatif stres ve hücre içi lipit, protein ve DNA yapıları üzerine etkileri. Journal of Clinical and Experimental Investigations. 2015;6(3):331-336.
  2. [2] Beckman KB, Ames BN. The free radical theory of aging matures. Physiological Reviews. 1998;78(2):547-581.
  3. [3] Aslankoç R, Demirci D, İnan Ü, Yıldız M, Öztürk A, Çetin M, et al. Oksidatif stres durumunda antioksidan enzimlerin rolü- Süperoksit dismutaz, katalaz ve glutatyon peroksidaz. SDÜ Tıp Fakültesi Dergisi. 2019;26(3):362-369.
  4. [4] Valko M, Leibfritz D, Moncol J, Cronin MTD, Mazur M, Telser J. Free radicals and antioxidants in normal physiological functions and human disease. The International Journal of Biochemistry and Cell Biology. 2007;39:44-84.
  5. [5] Kahköhnen MP, Hopia AI, Vuorela HJ, Rauha JP, Pihlaja K, Kujala TS, et al. Antioxidant activity of plant extracts containing phenolic compounds. Journal of Agricultural and Food Chemistry. 1999;47(10):3954-3962.
  6. [6] Karabulut H, Gülay M. Antioksidanlar. Mehmet Akif Ersoy Üniversitesi Veterinerlik Fakültesi Dergisi. 2016;1(1):65-76.
  7. [7] Pham-Huy LA, He H, Pham-Huy C. Free radicals, antioxidants in disease and health. International Journal of Biomedical Science. 2008;4(2):89-96.
  8. [8] Kaur C, Kapoor HC. Antioxidant activity and total phenolic content of some Asian vegetables. International Journal of Food Science and Technology. 2002;37(2):153-161.

Ayrıntılar

Birincil Dil

İngilizce

Konular

Bitki Bilimi (Diğer)

Bölüm

Araştırma Makalesi

Yayımlanma Tarihi

1 Ekim 2026

Gönderilme Tarihi

29 Ocak 2026

Kabul Tarihi

5 Temmuz 2026

Yayımlandığı Sayı

Yıl 2026 Cilt: 2026 Sayı: 17

Kaynak Göster

APA
Çolak, S., Işık, S., Kale Bakır, E., & Yüzügüllü Karakuş, Y. (2026). Extraction of Polyphenol Oxidase Enzyme of Black Mulberry (Morus nigra L.). Kocaeli Journal of Science and Engineering, 2026(17), 153-160. https://doi.org/10.34088/kojose.1875986
AMA
1.Çolak S, Işık S, Kale Bakır E, Yüzügüllü Karakuş Y. Extraction of Polyphenol Oxidase Enzyme of Black Mulberry (Morus nigra L.). KOJOSE. 2026;2026(17):153-160. doi:10.34088/kojose.1875986
Chicago
Çolak, Simge, Semih Işık, Elif Kale Bakır, ve Yonca Yüzügüllü Karakuş. 2026. “Extraction of Polyphenol Oxidase Enzyme of Black Mulberry (Morus nigra L.)”. Kocaeli Journal of Science and Engineering 2026 (17): 153-60. https://doi.org/10.34088/kojose.1875986.
EndNote
Çolak S, Işık S, Kale Bakır E, Yüzügüllü Karakuş Y (01 Ekim 2026) Extraction of Polyphenol Oxidase Enzyme of Black Mulberry (Morus nigra L.). Kocaeli Journal of Science and Engineering 2026 17 153–160.
IEEE
[1]S. Çolak, S. Işık, E. Kale Bakır, ve Y. Yüzügüllü Karakuş, “Extraction of Polyphenol Oxidase Enzyme of Black Mulberry (Morus nigra L.)”, KOJOSE, c. 2026, sy 17, ss. 153–160, Eki. 2026, doi: 10.34088/kojose.1875986.
ISNAD
Çolak, Simge - Işık, Semih - Kale Bakır, Elif - Yüzügüllü Karakuş, Yonca. “Extraction of Polyphenol Oxidase Enzyme of Black Mulberry (Morus nigra L.)”. Kocaeli Journal of Science and Engineering 2026/17 (01 Ekim 2026): 153-160. https://doi.org/10.34088/kojose.1875986.
JAMA
1.Çolak S, Işık S, Kale Bakır E, Yüzügüllü Karakuş Y. Extraction of Polyphenol Oxidase Enzyme of Black Mulberry (Morus nigra L.). KOJOSE. 2026;2026:153–160.
MLA
Çolak, Simge, vd. “Extraction of Polyphenol Oxidase Enzyme of Black Mulberry (Morus nigra L.)”. Kocaeli Journal of Science and Engineering, c. 2026, sy 17, Ekim 2026, ss. 153-60, doi:10.34088/kojose.1875986.
Vancouver
1.Simge Çolak, Semih Işık, Elif Kale Bakır, Yonca Yüzügüllü Karakuş. Extraction of Polyphenol Oxidase Enzyme of Black Mulberry (Morus nigra L.). KOJOSE. 01 Ekim 2026;2026(17):153-60. doi:10.34088/kojose.1875986