Soluble Receptor Level of Advanced Glycation (sRAGE) Products in Serum in Patients with Systemic Lupus Erythematosus:

Yıl 2024, Cilt: 19 Sayı: 2, 29 - 34, 22.07.2024

Serdar Gök , Burak Okyar , Defne Ay Tuncel , Fatma İnanç Tolun , Filiz Alkan Baylan , Can Acıpayam , Adem Doğaner , Gözde Yıldırım Çetin

https://doi.org/10.17517/ksutfd.1287352

Öz

Objectives: Systemic Lupus Erythematosus (SLE) is an autoimmune disease characterized by antibody formation against nuclear autoantigens. A receptor for Advanced Glycation (RAGE) is produced by many immune system cells, such as neutrophils, macrophages, and T cells, and interacts with many classes of ligands. In light of these results, the level of sRAGE, the soluble form of RAGE, may be associated with disease activity. In light of this information, we aimed to evaluate whether there is a relationship between plasma sRAGE levels and SLE.
Materials and Methods: Eighteen patients diagnosed with SLE (M/F: 1/17) and twenty-one patients without any disease diagnosis (M/F: 2/19) were included as the control group. In these patients, plasma sRAGE level was measured by ELIZA method using an ELIZA (enzyme-linked immunosorbent assay) kit (BioVendor Research and Diagnostic Products). The data obtained were compared between the groups.
Results: The mean plasma sRAGE level was lower in patients with SLE than in healthy control patients but not statistically significant (p=0.966). Our study found a positive correlation between SLEDAI and sRAGE levels in patients with SLE (r=0.628, p=0.005). Although no significant correlation was found between patients with SLE, sRAGE levels were positively correlated between fourteen patients classified as active SLE and the control group.
Conclusions: In our study, we found that plasma sRAGE levels in patients with SLE were lower than in healthy controls, but plasma sRAGE levels in patients with active SLE were higher than plasma sRAGE levels in patients with inactive SLE. We hypothesized that reduced sRAGE levels in patients with SLE could be explained by the depletion of this soluble receptor. Our study differed from another similar study showing that blood sRAGE levels were higher in patients with SLE than in healthy controls. Blood sRAGE levels were significantly increased during active disease compared with patients with quiescent SLE.

Anahtar Kelimeler

Systemic Lupus Erythematosus, sRAGE, Disease Activity

Sistemik Lupus Eritematozuslu Hastalarda Serumda Gelişmiş Glikasyon Ürünlerinin Çözünür Reseptörü (SRAGE) Seviyesi

Öz

Amaç: Sistemik Lupus Eritematozus (SLE) nükleer otoantijenlere karşı antikor oluşumu ile karakterize otoimmün bir hastalıktır. İleri Glikasyon Reseptörü (RAGE) nötrofiller, makrofajlar ve T hücreleri gibi birçok bağışıklık sistemi hücresi tarafından üretilir ve birçok ligand sınıfı ile etkileşime girer. Bu sonuçlar ışığında, RAGE'nin çözünebilir formu olan sRAGE seviyesi hastalık aktivitesi ile ilişkili olabilir. Bu bilgiler ışığında, plazma sRAGE düzeyleri ile SLE arasında bir ilişki olup olmadığını değerlendirmeyi amaçladık.
Gereç ve Yöntem: SLE tanısı konmuş on sekiz hasta (E/K: 1/17) ve herhangi bir hastalık tanısı olmayan yirmi bir hasta (E/K: 2/19) kontrol grubu olarak çalışmaya dahil edildi. Bu hastalarda plazma sRAGE düzeyi ELIZA (enzyme-linked immunosorbent assay) kiti (BioVendor Research and Diagnostic Products) kullanılarak ELIZA yöntemi ile ölçüldü. Elde edilen veriler gruplar arasında karşılaştırıldı.
Bulgular: Ortalama plazma sRAGE düzeyi SLE hastalarında sağlıklı kontrol hastalarına göre daha düşüktü ancak istatistiksel olarak anlamlı değildi (p=0.966). Çalışmamızda SLE'li hastalarda SLEDAI ve sRAGE düzeyleri arasında pozitif bir korelasyon bulundu (r=0.628, p=0.005). SLE'li hastalar arasında anlamlı bir korelasyon bulunmamasına rağmen, aktif SLE olarak sınıflandırılan on dört hasta ile kontrol grubu arasında sRAGE düzeyleri pozitif korelasyon göstermiştir.
Sonuç: Çalışmamızda, SLE'li hastalarda plazma sRAGE düzeylerinin sağlıklı kontrollere göre daha düşük olduğunu, ancak aktif SLE'li hastalarda plazma sRAGE düzeylerinin inaktif SLE'li hastalardaki plazma sRAGE düzeylerinden daha yüksek olduğunu bulduk. SLE'li hastalarda sRAGE düzeylerindeki azalmanın bu çözünür reseptörün tükenmesi ile açıklanabileceğini varsaydık. Çalışmamız, SLE'li hastalarda kan sRAGE düzeylerinin sağlıklı kontrollere göre daha yüksek olduğunu gösteren benzer bir başka çalışmadan farklıydı. Kan sRAGE düzeyleri, aktif hastalık sırasında, sakin SLE hastalarına kıyasla önemli ölçüde artmıştır.

Anahtar Kelimeler

Sistemik Lupus Eritematozus, sRAGE, Hastalık Aktivitesi

Kaynakça

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