Molecular Analyzing of Some Water Mite Species (Acari, Hydrachnidia) using DNA Barcodes

Öz

This study is the first DNA barcoding study with Hydrachna globosa, Hydryphantes dispar, Georgella helvetica and Hydrodroma despiciens which are very common species in the lakes (Acari, Hydrachnidia). Water mites samples used in the study were collected from Lake Karamik (Afyonkarahisar Turkey) between April to October 2015. Molecular analysis of the samples were carried out in the laboratory environment. The gDNA from each sample were isolated and the 28S rDNA gene region amplified. It was observed that the molecular analysis results obtained were in agreement with the classical systematic classification results for each species used in the study.

Anahtar Kelimeler

• Water Mites,DNA Barcoding,28S rDNA,Karamık Lake,Afyonkarahisar,Turkey

Kaynakça

1. Bader, C., 1975, ’’Die Wassermilben der Schweizerischen National Parks’’, I. Systematisch-faunistischer Ball, S.L. and Armstrong, K.F. (2006). DNA barcodes for insect pest identification: a test case with tussock moths (Lepidoptera: Lymantriidae). Canadian Journal of Forest Research, 36(2): 337-350.Bashasab, F., Vijaykumar, R., Kambapally, K.B., Patil, B.V. and Kuruvinashetti, M.S. (2006). DNA-based marker systems and their utility in entomology. Entomol. Fennica, 17: 21-33.Besansky, N.J., Severson, D.W. and Ferdig, M.T. (2003). DNA barcoding of parasites and invertebrate disease vectors: What you dont know can hurt you. Trends Parasitol, 19: 545-546.Boyacı, Y.Ö. (1995). Konya İli ve Çevresi Su Kenelerinin (Hydrachnellae, Acari) Sistematik Yönden İncelenmesi. Doktora Tezi, Atatürk Üniversitesi, Fen Bilimleri Enstitüsü. Erzurum.Campbell, B.C., Steffen-Campbell, J.D. and Werren, J.H. (1993). Phylogeny of the Nasonia species complex (Hymenoptera: Pteromalidae) inferred from an internal transcribed spacer (ITS2) and 28s rDNA sequences. Insect Molecular Biology, 2: 225–237.Dasmahapatra, K.K. and Mallet, J. (2006). DNA barcodes: Recent successes and future prospects. Heredity, 97(4): 254-255.Edwards, D.D., Vidrine, M.F. and Ernsting, B.R. (2010). Phylogenetic relationships among Unionicola (Acari: Unionicolidae) mussel-mites of North America based on mitochondrial cytochrome oxidase I sequences. Zootaxa, 2537(14): 47-57.Ernsting, B.R., Edwards, D.D., Vidrine, M.F., Myers, K.S. and Harmon, C.M. (2006). Phylogenetic relationships among species of the subgenus Parasitatax (Acari: Unionicolidae: Unionicola) based on DNA sequence of the mitochondrial cytochrome oxidase I gene. International Journal of Acarology, 32(2): 195-202.Ernsting, B.R., Edwards, D.D., Vidrine, M.F. and Cun, H. (2008). Genetic differences among sibling species of the subgenus Dimockatax (Acari: Unionicolidae: Unionicola): Heterogeneity in DNA sequence data supports morphological differentiation. International Journal of Acarology, 34(4): 403-407.Folmer, O., Black, M., Hoeh, W., Lutz, R. and Vrijenhoek, R. (1994). DNA primers for amplification of mitochondrial cytochrome coxidase subunit I from diverse metazoan invertebrates. Molecular Marine Biology and Biotechnology, 3(5): 294- 299.Gerson, U., Smiley, R.L. and Ochoa, R. (2003). Mites (Acari) for Pest Control.Blackwell Publishing, UK.Goolsby, J.A., De Barro, P.J., Makinson, J.R., Pemberton, R.W., Hartley, D.M. and Frohlich, D.R. (2006). Matching the origin of an invasive weed for selection of a herbivore haplotype for a biological control programme. Molecular Ecology, 15(1): 287-297.Gülle, P. (2010). Antalya ili Su Akarları (Hydrachnidia, Acari) Faunası. Doktora Tezi, Süleyman Demirel Üniversitesi, Su Ürünleri Temel Bilimleri, Isparta.Hebert, P.D., Cywinska, A. and Ball, S.L. (2003). Biological identifications through DNA barcodes. Proceedings of the Royal Society of London B: Biological Sciences, 270(1512): 313-321.Jinbo, U., Kato, T. and Ito, M. (2011). Current progress in DNA barcoding and future implications for entomology. Entomological Science, 14(2): 107-124.Kress, W.J. and Erickson, D.L. (2008). DNA barcodes: genes, genomics, and bioinformatics. Proceedings of the National Academy of Sciences, 105(8): 2761- 2762.Pilgrim, E.M., Jackson, S.A., Swenson, S., Turcsanyi, I., Friedman, E., Weigt, L. and Bagley, M.J. (2011). Incorporation of DNA barcoding into a large-scale biomonitoring program: opportunities and pitfalls. Journal of the North American Benthological Society, 30(1): 217-231.Ramadan, H.A. and Baeshen, N.A. (2012). Biological Identifications through DNA barcodes. In Lameed, G.A., (Eds), Biodiversity conservation and utilization in a diverse World, InTech Publisher, Republika Hrvatska, 109-129.Sites, J.W. and Marshall, J.C. (2003). Delimiting species: a Renaissance issue in systematic biology. Trends in Ecology and Evolution, 18(9): 462-470.Sites, J.W. and Marshall, J.C. (2004). Operational criteria for delimiting species. Annual Review of Ecology, Evolution, and Systematics, 35:199–227.Stålstedt, J., Bergsten, J. and Ronquist, F. (2013). Forms” of water mites (Acari: Hydrachnidia): intraspecific variation or valid species?. Ecology and Evolution, 3(10): 3415-3435.Walter, C., 1922b, ’’Hydracarinen aus den Alpen ’’, Rev. Suisse Zool., 29 (7), 228-411.Woese C.R. (1996). Phylogenetic trees: Whither microbiology? Current Biology, 6(9):1060-1063.Zhou, J., Davey, M.E., Figueras, J.B., Rivkina, E., Gilichinsky, D. and Tiedje, J.M. (1997). Phylogenetic diversity of a bacterial comminity determined srom Siberian tundra soil DNA. Microbiology, 143: 3913-3919.