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Van İlindeki Sokak Köpeklerinde Babesia Türlerinin Polimeraz Zincir Reaksiyonu Yöntemi İle Araştırılması

Yıl 2022, , 156 - 161, 31.05.2022

Ömer Selçin , Bekir Oğuz

https://doi.org/10.30934/kusbed.1097664
Cited By: 1

Öz

Amaç: Köpek babesiosisi Babesia spp.’ nin neden olduğu hemolitik anemi, splenomegali, trombositopeni ve ateşe neden olabilen kene kaynaklı bir protozoal hastalıktır. Türkiye’de yapılan moleküler çalışmalar soncunda B. canis, B. gibsoni, B. vogeli, B. rossi ve B. vulpes türlerinin varlığı ortaya konulmuştur. Yapılan literatür taramalarına göre Van ilinde yaşayan köpeklerde Babesia türlerini ortaya koyan herhangi bir çalışma bulunmamaktadır. Bu çalışma, Van ilindeki köpeklerde babesiosisin varlığı ve yaygınlığının Polimeraz Zincir Reaksiyonu (PZR) tekniği ile araştırılması amacıyla yapılmıştır.
Yöntem: Rastgele seçilen toplam 100 asemptomatik köpeğin vena cephalica antebrachii’lerinden EDTA’lı tüplere kan örnekleri alınmıştır. Alınan örneklerden elde edilen DNA’lar, Babesia spp.’ nin varlığı yönünden 18S ribozomal RNA geninin amplifiye edildiği PZR yöntemi ile araştırılmıştır.
Bulgular: Muayene edilen 100 köpeğin PZR sonuçlarına göre hiçbirinde Babesia spp. DNA’sına rastlanmamıştır.
Sonuç: Bu çalışma ile Van yöresi sokak köpeklerinde ilk kez Babesia spp. türleri moleküler olarak araştırılmıştır.

Anahtar Kelimeler

Babesia Sokak Köpeği Van polimeraz zincir reaksiyonu

Proje Numarası

Project No: TYL-2020-9169

Kaynakça

  • Solano-Gallego L, Sainz A, Roura X, Estrada-Peña A, Miró G. A review of canine-babesiosis: the European perspective. Parasit Vectors. 2016;9:336. doi: 10.1186/s13071-016-1596-0.
  • Vishwakarma P, Nandini MK. Overview of canine babesiosis. In: Mani S, Bekoe SO, Adosraku RK, Ramkumar PK, editors. Veterinary Medicine and Pharmaceuticals. United Kingdom: IntechOpen; 2019. p.109-125. doi: 10.5772/intechopen.82243.
  • Birkenheuer AJ, Levy MG, Breitschwerdt EB. Efficacy of combined atovaquone and azithromycin for therapy of chronic Babesia gibsoni (Asian genotype) infections in dogs. J Vet Intern Med. 2004;18(4):494-498. doi: 10.1892/0891-6640(2004)18<494:eocaaa>2.0.co;2.
  • Irwin PJ. Babesiosis and Cytauxzoonosis. In: Shaw SE, Day MJ, Editors. Arthropod-borne infectious diseases of the dog and cat. London: Manson Publishing/The Veterinary Press; 2005. p.63-77.
  • Altay K, Aktaş M, Dumanlı N. Köpek ve Kedilerde Görülen Parazit Hastalıklar. İn: Özcel MA, İnci A, Köroğlu E, Karaer Z, Eren H, Yukarı BA, Dumanlı N, Aydın L, Yıldırım A (Editors). Veteriner Hekimliğinde Parazit Hastalıkları. Cilt 2. İzmir: Meta Basım Matbaacılık; 2016.
  • Solano-Gallego L, Trotta M, Carli E, Carcy B, Caldin M, Furlanello T. Babesia canis canis and Babesia canis vogeli clinicopathological findings and DNA detection by means of PCR-RFLP in blood from Italian dogs suspected of tick-borne disease. Vet Parasitol. 2008;157:211-221. doi: 10.1016/j.vetpar.2008.07.024.
  • Solano-Gallego L, Baneth G. Babesiosis in dogs and cats -expanding parasitological and clinical spectra. Vet Parasitol. 2011;181:48–60. doi.org/10.1016/j.vetpar.2011.04.023.
  • Miro G, Checa R, Paparini A, Ortega N, Gonzalez-Fraga JL. Theileria annae (syn. Babesia microti-like) infection in dogs in NW Spain detected using direct and indirect diagnostic techniques: Clinical report of 75 cases. Parasit Vectors. 2015;8:217. doi.org/10.1186/s13071-015-0825-2.
  • Matjila PT, Leisewitz AL, Jongejan F, Penzhorn BL. Molecular detection of tick-borne protozoal and ehrlichial infections in domestic dogs in South Africa. Vet Parasitol. 2008;155:152-157. doi: 10.1016/j.vetpar.2008.04.012.
  • Gulanber A, Gorenflot A, Schetters TPM, Carcy B. First molecular diagnosis of Babesia vogeli in domestic dogs from Turkey. Vet Parasitol. 2006;139:224-230. doi.org/10.1016/j.vetpar.2006.02.035.
  • Aysul N, Ural K, Ulutas B, Eren H, Karagenc T. First detection and molecular identification of Babesia gibsoni in two dogs from the Aydin Province of Turkey. Turk J Vet Anim Sci. 2013;37:226-229. doi.org/10.3906/vet-1201-45.
  • Duzlu O, Inci A, Yildirim A, Onder Z, Ciloglu A. The investigation of some tick-borne protozoon and rickettsial infections in dogs by Real Time PCRand the molecular characterizations of the detected isolates. Ankara Univ Vet Fak Derg. 2014;61:275-282. doi.org/10.1501/Vetfak_0000002642.
  • Orkun Ö, Karaer Z. Molecular characterization of Babesia species in wild animals and their ticks in Turkey. Infect Genet Evol. 2017;55:8-13. doi: 10.1016/j.meegid.2017.08.026.
  • Aktas M, Özübek S, Altay K, İpek DNS, Balkaya İ, Ütük AE, et al. Molecular detection of tick-borne rickettsial and protozoan pathogens in domestic dogs from Turkey. Parasit Vectors. 2015;8:157. doi.org/10.1186/s13071-015-0763-z.
  • Aktas M, Ozubek S. A survey of canine haemoprotozoan parasites from Turkey, including molecular evidence of an unnamed Babesia. Comp Immunol Microbiol Infect Dis. 2017;52:36-42. doi:10.1016/j.cimid.2017.05.007.
  • Casati, SS, Sager, HH, Gern, LL, Piffaretti, JCJ-C. Presence of potentially pathogenic Babesia sp for human in Ixodes ricinus in Switzerland. Ann Agric Environ Med. 2006; 13(1):65.
  • Walker AR, Bouattour A, Camıcas JL, Estrada-Pena IG, Pegram RG, Preston PM. Ticks of domestic animals in Africa: a guide to identification of species. 1th ed. Edinburgh Scotland: U.K. The University of Edinburgh; 2003.
  • Estrada-Peña A, Bouattour A, Camicas JL, Walker AR. Ticks of veterinary and medical importance: The Mediterranean Basin. A guide of identification of species. Zaragoza: University of Zaragoza Press:2004.
  • Hatta T, Matsubayashi M, Miyoshi T, Islam K, Âlim MA, Anisuzzaman, et al. Quantitative PZR-based parasite burden estimation of Babesia gibsoni in the vector tick, Haemaphysalis longicornis (Acari: Ixodidae), fed on an experimentally infected dog. J Vet Med Sci. 2013;75:1-6. doi: 10.1292/jvms.12-0175.
  • Iwakami S, Ichikawa Y, Inokuma H. Molecular survey of Babesia gibsoni using Haemaphysalis longicornis collected from dogs and cats in Japan. J Vet Med Sci. 2014;76:1313-1316. doi: 10.1292/jvms.14-0210.
  • Jain J, Lakshmanan B, Nagaraj HV, Praveena JE, Syamala K, Aravindakshan T. Detection of Babesia canis vogeli, Babesia gibsoni and Ehrlichia canis by multiplex PCR in naturally infected dogs in South India. Vet Arhiv. 2018;88:215-224. doi: 10.24099/vet.arhiv.161229.
  • Apanaskevich DA, Horak IG, Camicas JL. Redescription of Haemaphysalis (Rhipistoma) elliptica (Koch, 1844), an old taxon of the Haemaphysalis (Rhipistoma) leachi group from East and southern Africa, and of Haemaphysalis (Rhipistoma) leachi (Audouin, 1826) (Ixodida, Ixodidae). Onderstepoort J Vet Res. 2007;74:181-208. doi: 10.4102/ojvr.v74i3.122.
  • Camacho AT, Pallas E, Gestal JJ, Guitian FJ, Olmeda AS, Telford SR, et al. Ixodes hexagonus is the main candidate as vector of Theileria annae in northwest Spain. Vet Parasitol. 2003;112:157-163. doi: 10.1016/s0304-4017(02)00417-x.
  • Bohm M, Leisewitz AL, Thompson PN, Schoeman JP. Capillary and venous Babesia canis rossi parasitaemias and their association with outcome of infection and circulatory compromise. Vet Parasitol. 2006;141:18-29. doi: 10.1016/j.vetpar.2006.05.002.
  • Jefferies R, Ryan UM, Muhlnickel CJ, Irwin PJ. Two Species of Canine Babesia in Australia: Detection and Characterization by PCR. J Parasitol. 2003;89(2):409-412. doi: 10.1645/0022-3395(2003)089[0409:TSOCBI]2.0.CO;2.
  • Sobczyk AS, Kotomski G, Górski P, Wędrychowicz H. Usefulness of touch-down PCR assay for the diagnosis of atypical cases of Babesia canis canis in dogs. Bull Vet Inst Pulawy. 2005; 49: 407-410.
  • Augustine S, Sabu L, Lakshmanan B. Molecular identification of Babesia spp. in naturally infected dogs of Kerala, South India. J Parasit Dis. 2017;41:459-462. doi: 10.1007/s12639-016-0827-9.
  • Ribeiro CM, Matos AC, Azzolini T, Bones ER, Wasnieski EA, Richini-Pereira VB, Lucheis SB, Vidotto O. Molecular epidemiology of Anaplasma platys Ehrlichia canis and Babesia vogeli in stray dogs in Paraná, Brazil. Pesqui Vet Brasil. 2017;37(2):129-136. doi.org/10.1590/S0100-736X2017000200006.
  • He L, Miao X, Hu J, Huang Y, He P, He J, et al. First molecular detection of Babesia gibsoni in dogs from Wuhan, China. Front Microbiol. 2017;8:1577. doi.org/10.3389/fmicb.2017.01577.
  • Barash NR, Thomas B, Birkenheuer AJ, Breitschwerdt EB, Lemler E, Qurollo BA. Prevalence of Babesia spp. and clinical characteristics of Babesia vulpes infections in North American dogs. J Vet Intern Med. 2019;33(5):2075-2081. doi: 10.1111/jvim.15560.
  • Checa R, Fidalgo LE, Montoya A, López AM, Barrera JP, Gálvez R, et al. The role of healthy dog carriers of Babesia microti-like piroplasms. Parasit Vectors. 2019;26: 12(1):127. doi.org/10.1186/s13071-019-3371-5.
  • Obeta SS, Ibrahim B, Lawal IA, Natala JA, Ogo NI, Balogun EO. Prevalence of canine babesiosis and their risk factors among asymptomatic dogs in the federal capital territory, Abuja, Nigeria. Parasite Epidemiol Control. 2020;11:e00186. doi.org/10.1016/j.parepi.2020.e00186.
  • Thomas RS, Santodomingo AM, Castro LR. Molecular detection of Babesia canis vogeli and Hepatozoon canis in dogs in the department of Magdalena, Colombia. Rev Med Vet Zoot. 2020;67(2): 7-122. doi.org/10.15446/rfmvz.v67n2.90701.
  • Gokce E, Kirmizigul AH, Taskin GT, Uzlu E, Gunduz N, Vatansever. Z: Clinical and Parasitological Detection of Babesia canis canis in Dogs: First Report from Turkey. Kafkas Univ Vet Fak Derg. 2013;19(4):717-720. doi:10.9775/kvfd.2013.7598.
  • Güven E, Avcıoğlu H, Cengiz S, Hayırlı A. Vector-Borne Pathogens in Stray Dogs in Northeastern Turkey. Vector Borne Zoonotic Dis. 2017;17(8):610-617. doi: 10.1089/vbz.2017.2128.
  • Guo H, Sevinç F, Ceylan O, Sevinç M, İnce E, Gao Y, et al. A PCR survey of vector-borne pathogens in different dog populations from Turkey. Acta Parasitol. 2017;62:533-540. doi: 10.1515/ap-2017-0064.
  • Aslantaş Ö, Çelebi B, Usluca S. Investigation of vector-borne diseases in dogs. Harran Univ Vet Fak Derg. 2020;9(2):154-160. doi:10.31196/huvfd.792147.
  • Gage KL, Burkot TR, Eisen RJ, Hayes EB. Climate and vectorborne diseases. Am J Prev Med. 2008;35:436-450. doi: 10.1016/j.amepre.2008.08.030.
  • Bakırcı S, Aktaş M, Vatansever Z, Aydın L. Keneler (Acarina: Ixodidae/Argasidae) Vektörlükleri ve Mücadelesi. Özbel Y editör. Vektör Artropodlar ve Mücadelesi. İzmir: Türkiye Parazitoloji Derneği, 2017. p.427-461.
  • Bilgic H, Pekel G, Hosgor M, Karagenc T. A Retrospective Epidemiological Study: The Prevalence of Ehrlichia canis and Babesia vogeli in Dogs in the Aegean Region of Turkey. Acta Vet. 2019;69(2):164-176. doi.org/10.2478/acve-2019-0013.
  • Orkun Ö. Molecular Characterization Based on 16S rDNA Phylogeny of Some Ixodid Ticks in Turkey. Turkiye Parazitol Derg. 2018;42:122-129. doi: 10.5152/tpd.2018.5882.
  • Deger MS, Bicek K, Oguz B. Infestation rate and distribution of hard ticks on cattle in the Eastern Anatolia Region of Turkey. Sci Parasitol. 2016;17:76-82.
  • Checa R, López-Beceiro AM, Montoya A, Barrera JP, Ortega N, Gálvez R, et al. Babesia microti-like piroplasm (syn. Babesia vulpes) infection in red foxes (Vulpes vulpes) in NW Spain (Galicia) and its relationship with Ixodes hexagonus. Vet Parasitol. 2018;252:22-28. doi: 10.1016/j.vetpar.2018.01.011.
  • Aydın MF, Aktas M, Dumanlı N. Tick Infestations on Sheep and Goats in the Black Sea Region of Türkiye. Kafkas Univ Vet Fak Derg. 2012;18 (Suppl-A):A17-A22. doi: 10.9775/kvfd.2011.5209.

Investigation of Babesia spp. in Stray Dogs in Van Province by Polymerase Chain Reaction

Yıl 2022, , 156 - 161, 31.05.2022

Ömer Selçin , Bekir Oğuz

https://doi.org/10.30934/kusbed.1097664
Cited By: 1

Öz

Amaç: Köpek babesiosisi Babesia spp.’ nin neden olduğu hemolitik anemi, splenomegali, trombositopeni ve ateşe neden olabilen kene kaynaklı bir protozoal hastalıktır. Türkiye’de yapılan moleküler çalışmalar soncunda B. canis, B. gibsoni, B. vogeli, B. rossi ve B. vulpes türlerinin varlığı ortaya konulmuştur. Yapılan literatür taramalarına göre Van ilinde yaşayan köpeklerde Babesia türlerini ortaya koyan herhangi bir çalışma bulunmamaktadır. Bu çalışma, Van ilindeki köpeklerde babesiosisin varlığı ve yaygınlığının Polimeraz Zincir Reaksiyonu (PZR) tekniği ile araştırılması amacıyla yapılmıştır.
Yöntem: Rastgele seçilen toplam 100 asemptomatik köpeğin vena cephalica antebrachii’lerinden EDTA’lı tüplere kan örnekleri alınmıştır. Alınan örneklerden elde edilen DNA’lar, Babesia spp.’ nin varlığı yönünden 18S ribozomal RNA geninin amplifiye edildiği PZR yöntemi ile araştırılmıştır.
Bulgular: Muayene edilen 100 köpeğin PZR sonuçlarına göre hiçbirinde Babesia spp. DNA’sına rastlanmamıştır.
Sonuç: Bu çalışma ile Van yöresi sokak köpeklerinde ilk kez Babesia spp. türleri moleküler olarak araştırılmıştır.

Anahtar Kelimeler

Babesia Stray Dogs Van polymerase chain reaction

Destekleyen Kurum

Van Yuzuncu Yil University Scientific Research Project Fund

Proje Numarası

Project No: TYL-2020-9169

Kaynakça

  • Solano-Gallego L, Sainz A, Roura X, Estrada-Peña A, Miró G. A review of canine-babesiosis: the European perspective. Parasit Vectors. 2016;9:336. doi: 10.1186/s13071-016-1596-0.
  • Vishwakarma P, Nandini MK. Overview of canine babesiosis. In: Mani S, Bekoe SO, Adosraku RK, Ramkumar PK, editors. Veterinary Medicine and Pharmaceuticals. United Kingdom: IntechOpen; 2019. p.109-125. doi: 10.5772/intechopen.82243.
  • Birkenheuer AJ, Levy MG, Breitschwerdt EB. Efficacy of combined atovaquone and azithromycin for therapy of chronic Babesia gibsoni (Asian genotype) infections in dogs. J Vet Intern Med. 2004;18(4):494-498. doi: 10.1892/0891-6640(2004)18<494:eocaaa>2.0.co;2.
  • Irwin PJ. Babesiosis and Cytauxzoonosis. In: Shaw SE, Day MJ, Editors. Arthropod-borne infectious diseases of the dog and cat. London: Manson Publishing/The Veterinary Press; 2005. p.63-77.
  • Altay K, Aktaş M, Dumanlı N. Köpek ve Kedilerde Görülen Parazit Hastalıklar. İn: Özcel MA, İnci A, Köroğlu E, Karaer Z, Eren H, Yukarı BA, Dumanlı N, Aydın L, Yıldırım A (Editors). Veteriner Hekimliğinde Parazit Hastalıkları. Cilt 2. İzmir: Meta Basım Matbaacılık; 2016.
  • Solano-Gallego L, Trotta M, Carli E, Carcy B, Caldin M, Furlanello T. Babesia canis canis and Babesia canis vogeli clinicopathological findings and DNA detection by means of PCR-RFLP in blood from Italian dogs suspected of tick-borne disease. Vet Parasitol. 2008;157:211-221. doi: 10.1016/j.vetpar.2008.07.024.
  • Solano-Gallego L, Baneth G. Babesiosis in dogs and cats -expanding parasitological and clinical spectra. Vet Parasitol. 2011;181:48–60. doi.org/10.1016/j.vetpar.2011.04.023.
  • Miro G, Checa R, Paparini A, Ortega N, Gonzalez-Fraga JL. Theileria annae (syn. Babesia microti-like) infection in dogs in NW Spain detected using direct and indirect diagnostic techniques: Clinical report of 75 cases. Parasit Vectors. 2015;8:217. doi.org/10.1186/s13071-015-0825-2.
  • Matjila PT, Leisewitz AL, Jongejan F, Penzhorn BL. Molecular detection of tick-borne protozoal and ehrlichial infections in domestic dogs in South Africa. Vet Parasitol. 2008;155:152-157. doi: 10.1016/j.vetpar.2008.04.012.
  • Gulanber A, Gorenflot A, Schetters TPM, Carcy B. First molecular diagnosis of Babesia vogeli in domestic dogs from Turkey. Vet Parasitol. 2006;139:224-230. doi.org/10.1016/j.vetpar.2006.02.035.
  • Aysul N, Ural K, Ulutas B, Eren H, Karagenc T. First detection and molecular identification of Babesia gibsoni in two dogs from the Aydin Province of Turkey. Turk J Vet Anim Sci. 2013;37:226-229. doi.org/10.3906/vet-1201-45.
  • Duzlu O, Inci A, Yildirim A, Onder Z, Ciloglu A. The investigation of some tick-borne protozoon and rickettsial infections in dogs by Real Time PCRand the molecular characterizations of the detected isolates. Ankara Univ Vet Fak Derg. 2014;61:275-282. doi.org/10.1501/Vetfak_0000002642.
  • Orkun Ö, Karaer Z. Molecular characterization of Babesia species in wild animals and their ticks in Turkey. Infect Genet Evol. 2017;55:8-13. doi: 10.1016/j.meegid.2017.08.026.
  • Aktas M, Özübek S, Altay K, İpek DNS, Balkaya İ, Ütük AE, et al. Molecular detection of tick-borne rickettsial and protozoan pathogens in domestic dogs from Turkey. Parasit Vectors. 2015;8:157. doi.org/10.1186/s13071-015-0763-z.
  • Aktas M, Ozubek S. A survey of canine haemoprotozoan parasites from Turkey, including molecular evidence of an unnamed Babesia. Comp Immunol Microbiol Infect Dis. 2017;52:36-42. doi:10.1016/j.cimid.2017.05.007.
  • Casati, SS, Sager, HH, Gern, LL, Piffaretti, JCJ-C. Presence of potentially pathogenic Babesia sp for human in Ixodes ricinus in Switzerland. Ann Agric Environ Med. 2006; 13(1):65.
  • Walker AR, Bouattour A, Camıcas JL, Estrada-Pena IG, Pegram RG, Preston PM. Ticks of domestic animals in Africa: a guide to identification of species. 1th ed. Edinburgh Scotland: U.K. The University of Edinburgh; 2003.
  • Estrada-Peña A, Bouattour A, Camicas JL, Walker AR. Ticks of veterinary and medical importance: The Mediterranean Basin. A guide of identification of species. Zaragoza: University of Zaragoza Press:2004.
  • Hatta T, Matsubayashi M, Miyoshi T, Islam K, Âlim MA, Anisuzzaman, et al. Quantitative PZR-based parasite burden estimation of Babesia gibsoni in the vector tick, Haemaphysalis longicornis (Acari: Ixodidae), fed on an experimentally infected dog. J Vet Med Sci. 2013;75:1-6. doi: 10.1292/jvms.12-0175.
  • Iwakami S, Ichikawa Y, Inokuma H. Molecular survey of Babesia gibsoni using Haemaphysalis longicornis collected from dogs and cats in Japan. J Vet Med Sci. 2014;76:1313-1316. doi: 10.1292/jvms.14-0210.
  • Jain J, Lakshmanan B, Nagaraj HV, Praveena JE, Syamala K, Aravindakshan T. Detection of Babesia canis vogeli, Babesia gibsoni and Ehrlichia canis by multiplex PCR in naturally infected dogs in South India. Vet Arhiv. 2018;88:215-224. doi: 10.24099/vet.arhiv.161229.
  • Apanaskevich DA, Horak IG, Camicas JL. Redescription of Haemaphysalis (Rhipistoma) elliptica (Koch, 1844), an old taxon of the Haemaphysalis (Rhipistoma) leachi group from East and southern Africa, and of Haemaphysalis (Rhipistoma) leachi (Audouin, 1826) (Ixodida, Ixodidae). Onderstepoort J Vet Res. 2007;74:181-208. doi: 10.4102/ojvr.v74i3.122.
  • Camacho AT, Pallas E, Gestal JJ, Guitian FJ, Olmeda AS, Telford SR, et al. Ixodes hexagonus is the main candidate as vector of Theileria annae in northwest Spain. Vet Parasitol. 2003;112:157-163. doi: 10.1016/s0304-4017(02)00417-x.
  • Bohm M, Leisewitz AL, Thompson PN, Schoeman JP. Capillary and venous Babesia canis rossi parasitaemias and their association with outcome of infection and circulatory compromise. Vet Parasitol. 2006;141:18-29. doi: 10.1016/j.vetpar.2006.05.002.
  • Jefferies R, Ryan UM, Muhlnickel CJ, Irwin PJ. Two Species of Canine Babesia in Australia: Detection and Characterization by PCR. J Parasitol. 2003;89(2):409-412. doi: 10.1645/0022-3395(2003)089[0409:TSOCBI]2.0.CO;2.
  • Sobczyk AS, Kotomski G, Górski P, Wędrychowicz H. Usefulness of touch-down PCR assay for the diagnosis of atypical cases of Babesia canis canis in dogs. Bull Vet Inst Pulawy. 2005; 49: 407-410.
  • Augustine S, Sabu L, Lakshmanan B. Molecular identification of Babesia spp. in naturally infected dogs of Kerala, South India. J Parasit Dis. 2017;41:459-462. doi: 10.1007/s12639-016-0827-9.
  • Ribeiro CM, Matos AC, Azzolini T, Bones ER, Wasnieski EA, Richini-Pereira VB, Lucheis SB, Vidotto O. Molecular epidemiology of Anaplasma platys Ehrlichia canis and Babesia vogeli in stray dogs in Paraná, Brazil. Pesqui Vet Brasil. 2017;37(2):129-136. doi.org/10.1590/S0100-736X2017000200006.
  • He L, Miao X, Hu J, Huang Y, He P, He J, et al. First molecular detection of Babesia gibsoni in dogs from Wuhan, China. Front Microbiol. 2017;8:1577. doi.org/10.3389/fmicb.2017.01577.
  • Barash NR, Thomas B, Birkenheuer AJ, Breitschwerdt EB, Lemler E, Qurollo BA. Prevalence of Babesia spp. and clinical characteristics of Babesia vulpes infections in North American dogs. J Vet Intern Med. 2019;33(5):2075-2081. doi: 10.1111/jvim.15560.
  • Checa R, Fidalgo LE, Montoya A, López AM, Barrera JP, Gálvez R, et al. The role of healthy dog carriers of Babesia microti-like piroplasms. Parasit Vectors. 2019;26: 12(1):127. doi.org/10.1186/s13071-019-3371-5.
  • Obeta SS, Ibrahim B, Lawal IA, Natala JA, Ogo NI, Balogun EO. Prevalence of canine babesiosis and their risk factors among asymptomatic dogs in the federal capital territory, Abuja, Nigeria. Parasite Epidemiol Control. 2020;11:e00186. doi.org/10.1016/j.parepi.2020.e00186.
  • Thomas RS, Santodomingo AM, Castro LR. Molecular detection of Babesia canis vogeli and Hepatozoon canis in dogs in the department of Magdalena, Colombia. Rev Med Vet Zoot. 2020;67(2): 7-122. doi.org/10.15446/rfmvz.v67n2.90701.
  • Gokce E, Kirmizigul AH, Taskin GT, Uzlu E, Gunduz N, Vatansever. Z: Clinical and Parasitological Detection of Babesia canis canis in Dogs: First Report from Turkey. Kafkas Univ Vet Fak Derg. 2013;19(4):717-720. doi:10.9775/kvfd.2013.7598.
  • Güven E, Avcıoğlu H, Cengiz S, Hayırlı A. Vector-Borne Pathogens in Stray Dogs in Northeastern Turkey. Vector Borne Zoonotic Dis. 2017;17(8):610-617. doi: 10.1089/vbz.2017.2128.
  • Guo H, Sevinç F, Ceylan O, Sevinç M, İnce E, Gao Y, et al. A PCR survey of vector-borne pathogens in different dog populations from Turkey. Acta Parasitol. 2017;62:533-540. doi: 10.1515/ap-2017-0064.
  • Aslantaş Ö, Çelebi B, Usluca S. Investigation of vector-borne diseases in dogs. Harran Univ Vet Fak Derg. 2020;9(2):154-160. doi:10.31196/huvfd.792147.
  • Gage KL, Burkot TR, Eisen RJ, Hayes EB. Climate and vectorborne diseases. Am J Prev Med. 2008;35:436-450. doi: 10.1016/j.amepre.2008.08.030.
  • Bakırcı S, Aktaş M, Vatansever Z, Aydın L. Keneler (Acarina: Ixodidae/Argasidae) Vektörlükleri ve Mücadelesi. Özbel Y editör. Vektör Artropodlar ve Mücadelesi. İzmir: Türkiye Parazitoloji Derneği, 2017. p.427-461.
  • Bilgic H, Pekel G, Hosgor M, Karagenc T. A Retrospective Epidemiological Study: The Prevalence of Ehrlichia canis and Babesia vogeli in Dogs in the Aegean Region of Turkey. Acta Vet. 2019;69(2):164-176. doi.org/10.2478/acve-2019-0013.
  • Orkun Ö. Molecular Characterization Based on 16S rDNA Phylogeny of Some Ixodid Ticks in Turkey. Turkiye Parazitol Derg. 2018;42:122-129. doi: 10.5152/tpd.2018.5882.
  • Deger MS, Bicek K, Oguz B. Infestation rate and distribution of hard ticks on cattle in the Eastern Anatolia Region of Turkey. Sci Parasitol. 2016;17:76-82.
  • Checa R, López-Beceiro AM, Montoya A, Barrera JP, Ortega N, Gálvez R, et al. Babesia microti-like piroplasm (syn. Babesia vulpes) infection in red foxes (Vulpes vulpes) in NW Spain (Galicia) and its relationship with Ixodes hexagonus. Vet Parasitol. 2018;252:22-28. doi: 10.1016/j.vetpar.2018.01.011.
  • Aydın MF, Aktas M, Dumanlı N. Tick Infestations on Sheep and Goats in the Black Sea Region of Türkiye. Kafkas Univ Vet Fak Derg. 2012;18 (Suppl-A):A17-A22. doi: 10.9775/kvfd.2011.5209.
Toplam 44 adet kaynakça vardır.

Ayrıntılar

Birincil Dil İngilizce
Konular Veteriner Cerrahi
Bölüm Özgün Araştırma / Veterinerlik Bilimleri
Yazarlar

Ömer Selçin 0000-0002-6462-9233

Bekir Oğuz 0000-0001-8720-3940

Proje Numarası Project No: TYL-2020-9169
Yayımlanma Tarihi 31 Mayıs 2022
Gönderilme Tarihi 2 Nisan 2022
Kabul Tarihi 26 Mayıs 2022
Yayımlandığı Sayı Yıl 2022

Kaynak Göster

APA Selçin, Ö., & Oğuz, B. (2022). Investigation of Babesia spp. in Stray Dogs in Van Province by Polymerase Chain Reaction. Kocaeli Üniversitesi Sağlık Bilimleri Dergisi, 8(2), 156-161. https://doi.org/10.30934/kusbed.1097664
AMA Selçin Ö, Oğuz B. Investigation of Babesia spp. in Stray Dogs in Van Province by Polymerase Chain Reaction. KOU Sag Bil Derg. Mayıs 2022;8(2):156-161. doi:10.30934/kusbed.1097664
Chicago Selçin, Ömer, ve Bekir Oğuz. “Investigation of Babesia Spp. In Stray Dogs in Van Province by Polymerase Chain Reaction”. Kocaeli Üniversitesi Sağlık Bilimleri Dergisi 8, sy. 2 (Mayıs 2022): 156-61. https://doi.org/10.30934/kusbed.1097664.
EndNote Selçin Ö, Oğuz B (01 Mayıs 2022) Investigation of Babesia spp. in Stray Dogs in Van Province by Polymerase Chain Reaction. Kocaeli Üniversitesi Sağlık Bilimleri Dergisi 8 2 156–161.
IEEE Ö. Selçin ve B. Oğuz, “Investigation of Babesia spp. in Stray Dogs in Van Province by Polymerase Chain Reaction”, KOU Sag Bil Derg, c. 8, sy. 2, ss. 156–161, 2022, doi: 10.30934/kusbed.1097664.
ISNAD Selçin, Ömer - Oğuz, Bekir. “Investigation of Babesia Spp. In Stray Dogs in Van Province by Polymerase Chain Reaction”. Kocaeli Üniversitesi Sağlık Bilimleri Dergisi 8/2 (Mayıs 2022), 156-161. https://doi.org/10.30934/kusbed.1097664.
JAMA Selçin Ö, Oğuz B. Investigation of Babesia spp. in Stray Dogs in Van Province by Polymerase Chain Reaction. KOU Sag Bil Derg. 2022;8:156–161.
MLA Selçin, Ömer ve Bekir Oğuz. “Investigation of Babesia Spp. In Stray Dogs in Van Province by Polymerase Chain Reaction”. Kocaeli Üniversitesi Sağlık Bilimleri Dergisi, c. 8, sy. 2, 2022, ss. 156-61, doi:10.30934/kusbed.1097664.
Vancouver Selçin Ö, Oğuz B. Investigation of Babesia spp. in Stray Dogs in Van Province by Polymerase Chain Reaction. KOU Sag Bil Derg. 2022;8(2):156-61.

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Van Veterinary Journal
https://doi.org/10.36483/vanvetj.1292352