Phytocomponent Profile, Antimicrobial, Antibiofilm, Antioxidant and Cytotoxic Activities of Gum Acanthus Obtained From Gundelia Tournefortii L. (Kenger) Plant

Öz

Objective: In our study, we aimed to investigate the phytoconstitutions of the mastic gum extract obtained from the seeds of the Gundelia tournefortii L. (Kenger) plant, which has many beneficial activities on human health, in terms of antimicrobial, antibiofilm, antioxidant activities and cytotoxic activities on SK-MEL-30 and A2058 human melanoma cell lines. Methods: In the study where phytoconstitutions were determined by GC-MS analysis, the antimicrobial activity of the extract was determined by the well diffusion method. While the DPPH method was used in the antioxidant activity study, cytotoxic activity was investigated on SK-MEL-30 and A2058 human melanoma cell lines with MTT test kit. Results: Mastic extract showed low MIC value compared to control group with strong antimicrobial activity. It was determined that the mastic extract showed 62.18% biofilm inhibition on E. coli ATCC 25922 strain and 48.24% biofilm inhibition on P. aeruginosa ATCC 278533 strain at the highest concentration of 20 mg/ml in antibiofilm activity. In our study where we investigated antioxidant activity with the DPPH method, the IC50 value of the mastic extract was determined as 35.70 mg/mL. In terms of cytotoxic activity, it was determined that the extract showed a strong growth inhibitory effect with an IC50 value of 11.07 μg/ml in SK-MEL-30 cells and showed high cytotoxic effect at doses of 50-1.8125 mg/mL in the A2058 cell line. Conclusion: These data contain valuable information as it is the first study to evaluate some biological activities of Kenger gum and will shed light on future studies.

Anahtar Kelimeler

• Gundelia tournefortii
• Kenger gum
• Phytocomponent
• Antimicrobial
• Antibiofilm
• Cytotoxic activities

Gundelia tournefortii L. (Kenger) Bitkisinden Elde Edilen Kenger Sakızının Fitokomponent Profili, Antimikrobiyal, Antibiyofilm, Antioksidan ve Sitotoksik Aktiviteleri

Öz

Amaç: Geçmişten günümüze tıbbi bitkilerin hastalıklar ve insan sağlığı üzerindeki yararlı etkileri bilim insanları tarafından araştırılmaya devam etmektedir. Bu bağlamda Gundelia tournefortii L. (Kenger) yüksek fenolik içeriği ve güçlü anti-inflamatuar, antioksidan ve potansiyel antikanser etkileri nedeniyle dikkat çekmektedir. Çalışmamızda, G. tournefortii L. bitkisinin tohumlarından elde edilen sakız özütünün fitokonstitüentleri, SK-MEL-30 ve A2058 insan melanoma hücre hatları üzerinde antimikrobiyal, antibiyofilm, antioksidan aktiviteleri ve sitotoksik aktiviteleri açısından araştırılması amaçlanmıştır. Gereç ve Yöntem: Fitokonstitüentlerin GC-MS analizi ile belirlendiği çalışmada, ekstratın antimikrobiyal aktivitsi kuyu difüzyon yöntemi ile antibiyofilm ve MIC değerleri 96 kuyucuklu mikroplatelerde seri dilüsyonlar yapılarak belirlenmiştir. Antioxidant aktivite çalışmasında DPPH yöntemi kullanılırken sitotoksik akitvitesi MTT test kiti ile SK-MEL-30 ve A2058 insan melanoma hücre hattı üzerinde araştırılmıştır. Bulgular: Sakız ekstratı, test edilen patojen mikroorganizmalar üzerinde güçlü antimikrobiyal aktivite göstermiş ve kontrol grubuna kıyasla düşük MİK değerleri göstermiştir. Sakız özütünün antibiyofilm aktivitesinde en yüksek 20 mg/ml konsantrasyonunda E. coli ATCC 25922 suşu üzerinde %62,18, P. aeruginosa ATCC 278533 suşu üzerinde ise %48,24 biyofilm inhibisyonu gösterdiği belirlenmiştir. DPPH yöntemi ile antioksidan aktiviteyi araştırdığımız çalışmamızda, sakız özütünün IC50 değeri 35,70 mg/mL olarak belirlenmiştir. Sitotoksik aktivite açısından ise, özütün SK-MEL-30 hücrelerinde 11,07 μg/ml IC50 değeri ile güçlü bir büyüme inhibitör etkisi gösterdiği, A2058 hücre hattında ise 50-1,8125 mg/mL dozlarında yüksek sitotoksik etki gösterdiği belirlenmiştir. Sonuç: Elde edilen bu veriler, kenger sakızının bazı biyolojik aktivitelerinin değerlendirildiği ilk çalışma olması nedeniyle değerli bilgiler içermekte olup, gelecekteki çalışmalara ışık tutacaktır.

Anahtar Kelimeler

• Gundelia tournefortii
• Kenger sakızı
• Fitokomponent
• Antimikrobiyal
• Antibiyofilm
• Sitotoksik aktivite

Kaynakça

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