Oxidant-Antioxidant Balance Changes in Adipose Tissues of High Fat Diet-Induced Obese Rats: Depot-Specific Manner and Ineffectiveness of N-Acetylcysteine

Yıl 2019, , 66 - 71, 20.05.2019

Cemil Kahraman , Ahmet Alver , Akın Bodur , İmran İnce Akça Diler Us Altay Sinan Canpolat

https://doi.org/10.30934/kusbed.498189

Öz

Objective: The aims of study
were to investigate effects of both N-acetylcysteine (NAC, an antioxidant) and
high fat diet (HFD) to oxidative stress in differently located adipose tissues
and the liver.

Methods: Our study is created from control,
HFD and NAC groups (n=6). Control group was fed with only standard diet. HFD
group was fed with only HFD. NAC group was fed with HFD, and additionally that
group received NAC (2 g/L). All groups were fed with designated diets for 85
days. Antioxidant enzyme activities, glutathione, and malondialdehyde were
measured in epididymal, perirenal, subcutaneous adipose tissues, and liver.

Results: In the HFD group, malondialdehyde
(MDA) levels increased in perirenal adipose tissue and liver. In addition,
superoxide dismutase (SOD) and catalase activities in the HFD group were lower
both epididymal and perirenal adipose tissue, whereas glutathione peroxidase
activities were lower in subcutaneous and epididymal adipose tissue.
Glutathione was lower in liver tissue alone. In subcutan adipose tissue, the
glutathione and SOD activities increased due to NAC administration.

Conclusion: The present findings showed that
oxidative stress and antioxidant enzyme profiles were variable in localized
adipose tissue in different regions. Thought antioxidant enzyme activities in
some tissues increased due to NAC application, these increases were
insignificant in terms of oxidant-antioxidant balance.

Anahtar Kelimeler

Adipose tissue, N-acetylcysteine, glutathione peroxidase, high fat diet, malondialdehyde, oxidative stress

Destekleyen Kurum

TUBITAK

Proje Numarası

111S252

Teşekkür

The financial fund of this study was provided by TUBITAK (111S252).

Yüksek Yağlı Diyetle İndüklenmiş Obez Sıçanların Yağ Dokularındakı Oksidan-Antioksidan Denge Değişiklikleri: Depoya Özgü Tutum ve N-Asetilsisteinin Etkisizliği

Öz

Amaç: Çalışmanın
amacı karaciğer ve farklı yerleşimli yağ dokularındaki oksidatif strese
N-asetilsisteinin (NAC, antioksidan) ve yüksek yağlı diyetin (HFD) etkilerini
araştırmaktır.

Yöntem: Çalışmamız
kontrol, HFD ve NAC gruplarından oluşmaktadır (n=6). Kontrol grubu standart diyetle,
HFD grubu yüksek yağ içeren diyetle, NAC grubu ise yüksek yağlı yem ve  içme suyuna katılan antioksidan molekül olan
NAC ile beslendi (2 g/L). Tüm gruplar 85 gün boyunca ad libitum olarak
beslendi. Beslenme periodunun sonunda karaciğer, subkutan, epididimal ve
perirenal yağ dokularında antioksidan enzimler (süperoksit dismutaz, katalaz,
glutatyon peroksidaz), glutatyon ve malondialdehit seviyeleri ölçüldü.

Bulgular: HFD
grubunda malondialdehit (MDA) seviyesi karaciğer ve perirenal yağ dokusunda
yüksekti. Ayrıca HFD grubunda süperoksit dismutaz (SOD) ve katalaz aktivitesi
hem epididimal hemde perirenal yağ dokusunda, glutatyon peroksidaz aktivitesi
epididimal ve subkutan yağ dokusunda düşüktü. Glutatyon ise sadece karaciğer
dokusunda düşük tesbit edildi.  NAC
uygulaması subkutan yağ dokusunda SOD ve glutatyonu yükseltti. 

Sonuç: Mevcut
bulgular oksidatif stres ve antioksidan enzim profillerinin farklı bölgelerde
yerleşmiş adipoz dokusunda değişken olduğunu, NAC uygulamasının bazı dokularda
antioksidan enzim aktivitelerini artırmasına rağmen oksidan-antioksidan denge
açısından önemli bir etkisinin olmadığını göstermektedir.

Anahtar Kelimeler

Adipoz doku, N-asetilsistein, glutatyon peroksidaz, yüksek yağlı diyet, malondialdehit, oksidatif stres

Proje Numarası

111S252

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