TAURİN TEDAVİSİNİN DENEYSEL MİYOZİT OLUŞTURULMUŞ RATLAR ÜZERİNDEKİ YARARLI ETKİLERİ

Öz

Amaç: Taurin iskelet kasında bol miktarda bulunan bir amino asittir. Hücre zarının korunması, anti-inflamatuar ve anti- apoptotik etkiler dahil olmak üzere sitoprotektif özelliklere sahiptir. Bildiğimiz kadarıyla, bu çalışma, taurinin asetik asit ile deneysel olarak oluşturulan miyozitteki kas hasarı ve inflamasyonu azaltmadaki faydalı etkilerini araştırmak amacıyla ilk kez yapılmıştır. Gereç ve Yöntemler: Çalışmaya 20 haftalık 24 adet Wistar albino türü sıçan dahil edildi. Sıçanlar kontrol grubu (n=8), %1 asetik asit (miyozit) grubu (n=8) ve miyozit + taurin 500 mg/kg/gün grubu (n=8) olmak üzere üç gruba ayrıldı. Miyozit indüksiyonu için %1 asetik asit kullanıldı. Taurin tedavisi 15 gün süreyle yapıldı ve ratlar sakrifiye edildi. Gastroknemius kası histopatolojik ve immünohistokimyasal olarak değerlendirildi. Elde edilen sonuçlar istatistiksel olarak karşılaştırıldı. Bulgular: Gruplar arasında konjesyon, dejenerasyon, nekroz, inflamasyon ve dezorganizasyon açısından istatistiksel olarak anlamlı fark vardı (p<0.01). Kaspaz3'te de istatistiksel olarak anlamlı fark vardı. Bu fark histopatolojik karşılaştırmadaki sonuçlara benzer şekilde asetik asit ve taurin grupları arasında p<0.01 idi. Sonuç: Taurinin anti-inflamatuar ve antioksidan özellikleri, kas hasarının ve inflamasyonun hafifletilmesine önemli katkılarda bulunabilir. Bu çalışmada, asetik asit ile miyozit oluşturulan sıçanlarda yapılan taurin tedavisinin, kas hasarının ve inflamasyonun belirgin bir şekilde azaldığını ve apoptozun baskılandığını gözlemledik. Bu sonuçlar, Taurinin inflamatuar kas hasarını azaltma konusundaki potansiyel faydalı etkilerini desteklemektedir.

Anahtar Kelimeler

• Taurin
• asetik asit
• deneysel miyozit
• apoptozis
• kas hasarı

Destekleyen Kurum

Hiçbir kişi/kuruluş çalışmayı finansal olarak desteklememiştir.

Etik Beyan

Bu çalışma Sağlık Bilimleri Üniversitesi Ankara Eğitim ve Araştırma Hastanesi Hüsnü Sakal Deneysel ve Klinik Uygulama Merkezi'nde deney hayvan kullanımına ilişkin etik kurallara uygun olarak yapılmıştır. Deneysel protokoller için Yerel Hayvan Deneyleri Etik Kurulundan etik onay alınmıştır (724/2023).

Teşekkür

Çalışmamızın histopatolojik ve immunhistokimyasal parametrelerinin değerlendirilmesinde, değerli katkılarından dolayı Harran Üniversitesi Veteriner Fakültesi Patoloji Anabilim Dalı'ndan Vet. Doç. Dr. Nihat Yumuşak'a sonsuz teşekkürler.

Beneficial Effects of Taurine Treatment on Experimental Myositis-induced Rats

Öz

Objective: Taurine is an amino acid abundantly present in skeletal muscle, and it possesses cytoprotective properties, including the preservation of cell membrane integrity, anti- inflammatory, and anti-apoptotic effects. To the best of our knowledge, this study was conducted for the first time to investigate the potential beneficial effects of taurine in reducing muscle damage and inflammation in experimentally induced myositis using acetic acid. Material and Methods: The study included 24 Wistar albino rats aged 20 weeks. The rats were divided into three groups: a control group (n=8), an acetic acid (myositis) group (n=8), and a myositis + taurine 500 mg/kg/day group (n=8). Acetic acid at a concentration of 1% was used for myositis induction. Taurine treatment was administered for 15 days, after which the rats were sacrificed. The gastrocnemius muscle was evaluated histopathologically and immunohistochemically, and the results were compared statistically. Results: Significant differences were observed between the groups in terms of congestion, degeneration, necrosis, inflammation, and disorganization (p<0.01). There was also a statistically significant difference in caspase-3 levels. This difference was consistent with the histopathological findings, with p<0.01 between the acetic acid and taurine groups. Conclusion: Taurines anti-inflammatory and antioxidant properties may significantly contribute to mitigating muscle damage and inflammation. In this study, we observed a marked reduction in muscle damage and inflammation, along with the suppression of apoptosis, in rats with myositis induced by acetic acid following taurine treatment. These findings support the potential beneficial effects of taurine in reducing inflammatory muscle damage.

Anahtar Kelimeler

• Taurin
• acetic acid
• experimental myositis
• apoptosis
• muscle damage

Kaynakça

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