İNSAN ENDOMETRİOİD OVER ADENOKARSİNOM HÜCRE HATTI MDAH-2774 HÜCRELERİNDE KARNOSOL VE KARNOSİK ASİTİN SİTOTOKSİK ETKİSİNİN VE OLASI MEKANİZMALARININ DEĞERLENDİRİLMESİ

Öz

Amaç: Over kanseri, kadınlarda en yüksek ölüm oranına sahip olan ve genellikle ileri evrede teşhis edilebilen bir jinekolojik kanser türüdür. Kanser hücrelerini duyarlı hale getirmek için sitotoksik tedaviler araştırılmaktadır. Sitotoksik etkileri olduğu bilinen bitki kaynaklı ürünlerin kullanımı, kanser büyümesini baskılamada ilgi çekici bir yöntem haline gelmiştir. Hem karnosol hem de karnosik asit, antioksidan, anti-inflamatuvar, anti-proliferatif ve antikanser aktivitelere sahip olan bitkisel diterpenoidlerdir. Yaptığımız bu çalışmanın amacı hem karnosol hem de karnosik asidin MDAH-2774 over endometrioid kanseri hücre hattı üzerindeki etkilerini araştırmaktır. Gereç ve Yöntemler: Çalışmamızda farklı konsantrasyonlar ve zaman aralıkları kullanılmıştır. Karnosol ve/veya karnosik asidin varlığında ve yokluğunda hücre canlılığı üzerine olan etkiler, MTT analizi ile saptanmıştır. Ajanların hücre canlılığı üzerindeki etkilerinin ardındaki mekanizmayı anlamak için hücrelerin toplam antioksidan durumu değerlendirilmiştir. Bulgular: Sonuçlarımız, ajanların tek başlarına ve daha çok da kombine halde MDAH-2774 hücre canlılığını başarıyla azalttığını göstermiştir. Toplam antioksidan durum analizi bileşiklerin ayrı ayrı ve kombine halde antioksidan kapasitelerinin yüksekliğini göstermiştir. Sonuç: Çalışmamız, karnosol ve karnosik asidin tek başına ve kombine halde gösterdikleri sitotoksik etkinin onların antioksidan etkileriyle ilişkili olabileceğini ve bu etkinin kombine halde daha güçlü olduğunu saptamıştır.

Anahtar Kelimeler

• Karnosol
• karnosik asit
• MDAH-2774
• over kanseri
• sitotoksik etki
• total antioksidan durum

Evaluation of the Cytotoxic Effect and Possible Mechanisms of Carnosol and Carnosic Acid in Human Endometrioid Ovarian Adenocarcinoma Cell Line MDAH-2774 Cells

Öz

Objective: Ovarian cancer is one of the most lethal forms of gynecological malignancies in women and is often detected at an advanced stage. Cytotoxic treatments have been investigated to sensitize cancer cells. Naturally occurring products are becoming an attractive method for suppressing cancer growth because of their cytotoxic properties. Both carnosol and carnosic acid are diterpenoids with antioxidant, anti- inflammatory, anti-proliferative, and anti-cancer activities. The present study aims to investigate the effects of both carnosol and carnosic acid on the MDAH-2774 cell line. Material and Methods: Different concentrations and time intervals were used in the present study. We employed MTT to show cell survival in the presence and absence of carnosol and/or carnosic acid. To understand the mechanisms behind the effects of the agents on cell survival, total antioxidant capacity was evaluated. Results: Our results showed that the agents, alone and more so in combination, successfully reduced the viability of MDAH- 2774 cells. Total antioxidant capacity analysis revealed that the compounds had high antioxidant capacities both when used alone and when combined. Conclusion: Our study determined the cytotoxic effects of carnosol and carnosic acid, both when used alone and in combination. These effects may be attributed to their antioxidant properties, with the combined form exhibiting a more pronounced impact.

Anahtar Kelimeler

• Carnosol
• carnosic acid
• MDAH-2774
• ovarian cancer
• cytotoxic effect
• total antioxidant status

Kaynakça

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