DENEYSEL TESTİS TORSİYONU SONRASI İSKEMİ/REPERFÜSYON HASARINDA İLOPROST VE DÜŞÜK DOZ METOTREKSAT’IN KORUYUCU ETKİSİ

Yıl 2017, , 131 - 138, 18.12.2017

Ercan Yuvanç , Devrim Tuğlu , Üçler Kısa Önder Bozdoğan , Ertan Batislam Erdal Yılmaz

https://doi.org/10.24938/kutfd.336398

Öz

Amaç: Testis torsiyonunda cerrahi olarak
detorsiyone edilen testiste gelişen iskemi/reperfüzyon (I/R) hasarı üzerine
İloprost ve düşük doz Metotreksat’ın koruyucu etkilerini araştırmak.

Gereç ve Yöntem: Ratlar rastgele 4 gruba ayrıldı.
Grup 1 (sham), Grup 2 = İskemi/Reperfüzyon, ilaç verilmedi. Grup 3 =
İskemi/Reperfüzyon + İloprost (10 µg/kg) ve Grup 4 = İskemi/Reperfüzyon + Düşük
doz Metotreksat (6 mg/kg). Torsiyon/iskemi ve detorsiyon/reperfüzyon süreleri
4’er saat olarak belirlenmiştir. Medikasyonlar intraperitoneal yolla testis
torsiyonunu takiben 3. saatte verilmiştir. Her 4 grupta işlemin başlangıcından
8 saat sonra detorsiyone sağ testis çıkarılmıştır.

Bulgular: Çalışmamızda, iloprost ve düşük doz
metotreksat verilmesinin total antioksidan kapasiteyi (TAK) istatistiksel
olarak anlamlı arttırdığını, oksidan kapasite markırı olan total oksidan kapasite
(TOK) ve oksidatif stres indeks oranını (OSI) ise istatistiksel olarak anlamlı
düzeyde azalttığını belirledik. İskemi-reperfüzyon grubu testislerde testis
grupları arasında Johnsen skorlama sistemine göre istatistiksel farklılık
saptanmadı.

Sonuç: Bizim düşüncemize göre iloprost ve
düşük doz metotreksat tedavisi, testis örneklerinde antioksidan aktiviteyi
arttırmış, oksidan aktiviteyi de baskılamıştır. Ancak, histopatolojik
incelemede ve spermatogenes skorlarında bir değişime neden olmamıştır.

Anahtar Kelimeler

Testis torsiyonu, detorsiyon, antioksidan, iskemi/reperfüzyon, iloprost, düşük doz metotreksat

The Protective Effect of Iloprost and Low-Dose Methotrexate on Testicular Ischemia/Reperfusion Injury in an Experimental Model

Öz

Objective: To investigate the protective effects of iloprost and
low-dose methotrexate on ischemia/reperfusion (I/R) injury in surgically
detorsed testicular torsion.

Material and Methods: Rats were randomly divided into 4 groups: Group 1 (sham),
Group 2: Only testicular I/R; Group 3: I/R+iloprost (10 μg/kg) and Group 4:
I/R+low dose metotrexate (6 mg/kg). Torsion / ischemia and detorsion /
reperfusion times were determined as 4 hours. Medications were given
intraperitoneally at 3 hours following onset of testicular torsion. Detorsioned
right testises were removed 8 hours after the begining of the procedure in all
4 groups.

Results: In our study, we found that administration of iloprost and
metotrexate significantly increased total antioxidant capacity (TAC) while
reducing total oxidant statüs (TOS) and oxidative stress index (OSI) levels
significantly. No statistically significant difference was found for testicles
in I/R groups according to the Johnsen scoring system.

Conclusion: Admnistration of iloprost and low dose methotrexate treatment
increased antioxidant activity in testicular samples while suppressing oxidant
activity. However, there was no change in histopathological examination and
spermatogenesis scores.

Anahtar Kelimeler

Testis torsion, detorsion, antioxidant, ischemia/reperfusion, iloprost, methotrexate

Kaynakça

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