Afyonkarahisar'da Satışa Sunulan Kaymaklardan İzole Edilen Staphylococcus aureus Suşlarında Metisilin ve Panton-Valentine Lökosidin Genlerinin Araştırılması

Öz

Bu çalışmada, Afyonkarahisar'da üretilen ve satılan kaymak örneklerinden izole edilen Staphylococcus aureus suşlarında metisilin ve Panton-Valentine lökosidin genlerinin araştırılması amaçlandı. Kasım 2019 ile Aralık 2020 tarihleri arasında Afyonkarahisar halk pazarlarında satışa sunulan toplam 110 kaymak örneği toplandı. Kaymak örneklerinden S. aureus izolasyonu konvansiyonel kültür yöntemleri kullanılarak gerçekleştirildi. Örneklerden izole edilen S. aureus suşlarının doğrulanması ve suşlarda mecA ve pvl genlerinin belirlenmesi amacıyla PZR kullanıldı. Çalışmada 110 kaymak örneğinin 14'ünden standart kültürel yöntemlerle S. aureus izole edilirken, 110 örneğin 13'ü (%11,8) PZR ile S. aureus olarak tiplendirildi. On üç S. aureus suşunun hiçbirinde mecA ve pvl genleri bulunmadı. Türkiye'de kaymaklardan izole edilen S. aureus suşlarında ilk kez pvl geninin araştırıldığı bu çalışmada, bu geleneksel ürün ve diğer süt ürünlerinde mecA ve pvl genlerinin belirlenmesi için daha fazla araştırma yapılması gerektiği düşünüldü.

Anahtar Kelimeler

• Kaymak
• mecA
• pvl
• Staphylococcus aureus

Investigation of Methicillin and Panton-Valentine Leukocidin Genes in Staphylococcus aureus Strains Isolated from Clotted Creams Sold in Afyonkarahisar

Abstract

This study aimed to investigate the methicillin and Panton-Valentine leukocidin genes in Staphylococcus aureus strains isolated from clotted cream samples produced and sold in Afyonkarahisar. A total of 110 clotted cream samples sold in public bazaars of Afyonkarahisar were collected between November 2019 and December 2020. Conventional cultural methods achieved the isolation of S. aureus from clotted cream samples. For the confirmation of S. aureus strains isolated from samples and determination of mecA and pvl genes in the strains, PCR was used. In this study, while S. aureus was isolated from 14 of 110 clotted cream samples by standard cultural methods, 13 (11.8%) of 110 samples were typed to be S. aureus by PCR. The mecA and pvl genes were found in none of the 13 S. aureus strains. In this study, in which the pvl gene was investigated for the first time in the S. aureus strains isolated from clotted creams in Turkey, it was thought that more research should be done to determine mecA and pvl genes in this traditional product and other dairy products.

Keywords

• Clotted cream
• mecA
• pvl
• Staphylococcus aureus

Kaynakça

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