Burdur Yöresi Honamlı Irkı Keçilerde Schmallenberg Virüs Seroprevalansının Araştırılması

Öz

Schmallenberg virüsü (SBV) ilk olarak Kasım 2011'de kuzeybatı Almanya'da ve Hollanda'nın doğusunda sığırlarda rapor edilmiştir. SBV evcil geviş getiren hayvanlarda ciddi ekonomik kayıplara yol açmaktadır. SBV ülkemizde de görülmektedir ve sığırlarda ve koyunlarda ve keçilerde az sayıda seroprevalans çalışmaları bulunmaktadır. Fakat Honamlı ırkı keçilerde yapılan seroprevalas çalışması yoktur. Bu amaçla bu çalışmada Burdur yöresinde Honamlı ırkı keçilerde SBV prevalansı araştırılmıştır. Bu çalışma Burdur ilinde Honamlı keçisi yetiştiren 12 farklı sürüde yürütülmüştür. Hayvan materyalini 2-5 yaşları arasında değişen 93 abortlu ve 93 normal sağlıklı doğum yapan 186 Honamlı keçi oluşturmuştur. Bulgularda; Çalışmaya dahil edilen hayvandan kan serumlarının yalnızca ikisinde (% 1.1) Schmallenberg virus spesifik antikor varlığı saptandı. iki keçide ise (% 1.1) test sonucu şüpheli antikor pozitiflik belirlenmiştir. Sonuç olarak; Burdur ilinde Honamlı ırkı keçilerde SBV enfeksiyonunun düşük seroprevalans oranına sahip olduğu belirlenmiştir.

Anahtar Kelimeler

• ELISA
• Honamlı Keçisi
• Keçi
• Schmallenberg virus
• Seroprevalans

Investigation of Schmallenberg Virus Seroprevalence in Honamlı Goats from Burdur Region

Öz

Schmallenberg virus (SBV) was first reported in cattle, in north-western Germany and east of the Netherlands in November 2011. SBV causes serious economic losses in domesticated ruminant animals. SBV is also seen in Turkey and there are few seroprevalence studies in cattle, sheep and goats. For this purpose, in this study, the prevalence of SBV was investigated in Honamlı goats in Burdur region. The study was carried out in 12 different herds that were breeding Honamlı goats in Burdur province. The animal material consisted of 186 Honamlı goats with 93 aborted and 93 normal healthy births which were aged between 2-5 years. In the findings; SBV specific antibodies were detected in only two (1.1 %) serum samples from the animals included in the study. In two goats (1.1 %), the test result was suspectable for antibody positivity. Consequently, it was determined that SBV infection has a low seroprevalence rate in Honamlı goats in Burdur province.

Anahtar Kelimeler

• ELISA
• Goat
• Honamlı Goat
• Schmallenberg virus
• Seroprevalence

Kaynakça

1. Azkur AK, Albayrak H, Risvanli A, Pestil Z, Ozan E, Yılmaz O, Tonbak S, Cavunt A, Kadı H, Macun HC, Acar D, Özenç E, Alparslan S, Bulut H, Antibodies to Schmallenberg virus in domestic livestock in Turkey. Trop Anim Health Prod. 2013; 45: 1825-1828.
2. Azkur AK, van der Poel WH, Aksoy E, Hakze-van der Honing R, Yildirim M, Yıldız K. Development and validation of SYBR Green-and probe-based reverse-transcription real-time PCR assays for detection of the S and M segments of Schmallenberg virus. J Vet Diagn Invest. 2020; 32(5): 710-717.
3. Brülisauer F, Scholes S, Caldow GL, Rocchi M, Dagleish MP, Chianini F. Role of Schmallenberg virus infection in congenital malformations in ruminants in Scotland in spring 2017. Vet Rec. 2017; 181(13): 341-343.
4. Conraths FJ, Peters M, Beer M, Schmallenberg virus, a novel orthobunyavirus infection in ruminants in Europe: Potential global impact and preventive measures. N Z Vet J. 2013; 61(2): 63-67.
5. Elbers AR, Loeffen WL, Quak S, de Boer-Luijtze E, van der Spek AN, Bouwstra R, Maas R, Spierenburg MAH, de Kluijver EP, van Schaik G,van der Poel WH. Seroprevalence of Schmallenberg virus antibodies among dairy cattle, the Netherlands, winter 2011–2012. Emerging infectious diseases. 2012; 18(7): 1065.
6. Elbers AR, Stockhofe-Zurwieden N, van der Poel WH, Schmallenberg virus antibody persistence in adult cattle after natural infection and decay of maternal antibodies in calves. BMC Vet Res, 2014; 10: 103.
7. Elmas A, Aslan Ö, Şahna KC. Sivas Yöresindeki Koyunlarda Schmallenberg Virus Enfeksiyonunun Seroprevalansının Belirlenmesi. Harran Üniv Vet Fak Derg. 2018; 7(1): 56-59. Endalew AD, Faburay B, Trujillo JD, Gaudreault NN, Davis AS, Shivanna V, Sunwoo SY, Ma W, Drolet BS, McVey DS, Morozov I, Wilson WC, Richt JA. Immunogenicity and efficacy of Schmallenberg virus envelope glycoprotein subunit vaccines. J Vet Sci. 2019; 20(6): 1-11.
8. Jiménez‐Martín D, Cano‐Terriza D, Díaz‐Cao JM, Pujols J, Fernández‐Morente M, García‐Bocanegra I. Epidemiological surveillance of Schmallenberg virus in small ruminants in southern Spain. Transbound Emerg Dis. 2021; 68(4): 2219-2228.

9. Kauffold J, Hoops M, Vahlenkamp TW. Infectious Agents: Schmallenberg Virus. In Bovine Reproduction Ed; Richard M. Hopper DVM, Diplomat ACT, 2nd Ed., John Wiley & Sons, Inc., Hoboken, NJ, USA. 2021; pp. 784-792.
10. Kęsik-Maliszewska J, Antos A, Rola J, Larska M. Comparison of Schmallenberg virus sequences isolated from mammal host and arthropod vector. Virus genes. 2018; 54(6): 792-803.
11. Lievaart-Peterson K, Luttikholt S, Peperkamp K, Van den Brom R, Vellema P. Schmallenberg disease in sheep or goats: Past, present and future. Vet Microbiol. 2015; 181(1-2): 147-153.
12. Macun HC, Azkur AK, Kalender H, Erat S. Kırıkkale'de yetiştirilen koyunlarda Schmallenberg virüs seroprevalansı ve bazı coğrafi özelliklerle ilişkisi. Ankara Üniv Vet Fak Derg. 2017; 64: 93-97.
13. Pejaković S, Wiggers L, Coupeau D, Kirschvink N, Mason J, Muylkens B. Test selection for antibody detection according to the seroprevalence level of Schmallenberg virus in sheep. PloS one. 2018; 13(4): e0196532.
14. Pawaiya RVS, Gupta VK. A review on Schmallenberg virus infection: A newly emerging disease of cattle, sheep and goats. Vet Med-Czech. 2013; 58: 516-526. Szeredi L, Dán Á, Malik P, Jánosi S, Hornyák Á. Low incidence of Schmallenberg virus infection in natural cases of abortion in domestic ruminants in Hungary. Acta Vet Hung. 2020; 6 (1):105–111.
15. Tonbak Ş, AZKUR AK, Pestil Z, Biyikli E, Abayli H, Baydar E, Bulut H. Circulation of Schmallenberg virus in Turkey, 2013. Turk J Vet Anim Sci. 2016; 40(2): 175-180. Wernike K, Beer M. Re‐circulation of Schmallenberg virus, Germany, 2019. Transbound Emerg Dis. 2020; 67(6): 2290-2295.
16. Wernike K, Holsteg M, Szillat KP, Beer M. Development of within-herd immunity and long-term persistence of antibodies against Schmallenberg virus in naturally infected cattle. BMC veterinary research. 2018; 14(1): 1-6.
17. Yilmaz H, Hoffmann B, Turan N, Cizmecigil UY, Richt JA, Van der Poel WH. Detection and partial sequencing of Schmallenberg virus in cattle and sheep in Turkey. Vector Borne Zoonotic Dis. 2014; 14(3): 223-225.