Evaluation of the Effect of Panax Ginseng Administration on YKG1 Glioblastoma Cells Before Cisplatin Treatment in Terms of Apoptotic Cell Death and Cell Vitality

Öz

In alternative medicine, natural remedies of herbal origin are often used in addition to chemotherapy-like treatments. Panax ginseng one of them and it’ s preferred diseases originating from nervous system. The aim of our study, to evaluate Panax ginseng, which was used before cisplatin, in terms of cell death and viability YKG1 cells. Commercially, purchased YKG1 cells were incubated DMEM F12 medium in 5% CO2 incubator divided into 4 groups. Group-1; control, group-2; 48 hours 50 mg/ml Panax ginseng, group-3; 48 hours 50 mg/ml Panax ginseng followed by Cisplatin treatment and group-4; was determined as the only cisplatin-treated group. Cell viability assessed by MTT. Bax/Bcl-2 and Caspase3 primary antibodies, were used for immunocytochemical evaluation. In addition, cells were evaluated by PCR tecnique with using primers Bcl-2 and Caspase-3. According to the immunocytochemistry, while Bax immunoreactivity didn’t change into the groups, Bcl-2 decreased in groups 3-4, and Caspase-3 increased in groups 3-4, and PCR results were also supportive with our immunohistochemical results. According to MTT results, cell viability rates decreased in groups 3-4. In our study, it was observed that the use of Panax ginseng, which used as a therapeutic and prophylactic in alternative medicine, didn’t change the Bax/Bcl-2 ratio in YKG1 cells, but it could trigger apoptosis increasing Caspase-3 activity together Cisplatin.

Anahtar Kelimeler

• Bax/Bcl
• Caspase 3
• Cisplatin
• Glioblastoma

Sisplatin Tedavisi Öncesi Panax Ginseng Uygulamasının YKG1 Glioblastoma Hücrelerine Olan Etkisinin Apoptotik Hücre Ölümü Ve Hücre Canlılığı Açısından Değerlendirilmesi

Öz

Alternatif tıpta kemoterapi benzeri tedavi yöntemlerine ek olarak sıklıkla bitkisel kökenli doğal ilaçlar kullanılmaktadır. Panax ginseng bunlardan biridir ve sinir sistemi kaynaklı hastalıklarda tercih edilmektedir. Çalışmamızda amaç, sisplatinden önce kullanılan Panax ginsengin YKG1 glioblastoma hücrelerinde hücre ölümü ve hücre canlılığı bakımından değerlendirilmesidir. Ticari olarak satın alınan YKG1 hücreleri, % 5 CO2 inkübatörde DMEM F12 besi yerinde inkübe edildi ve 4 gruba ayrıldı. Grup 1; kontrol, grup 2; 48 saat 50 mg/ml Panax ginseng, grup 3; 48 saat 50 mg/ml Panax ginseng ve ardından Sisplatin tedavisi ve grup 4; sadece Sisplatin uygulanan grup olarak belirlendi. Hücre canlılığı MTT analizi ile değerlendirildi. İmmünositokimyasal değerlendirmede apoptotik belirteçlerden Bax/Bcl-2 ve Caspase-3 primer antikorları kullanıldı. Ek olarak, hücreler PCR tekniği ile Bcl-2 ve Caspase-3 primerleriyle değerlendirildi. İmmünositokimyasal sonuçlara göre Bax immünoreaktivitesi gruplar arasında değişiklik göstermezken, Bcl-2’nin grup 3 ve 4’te azaldığı ve Caspase-3’ün ise grup 3 ve 4'te arttığı görüldü ve PCR sonuçları da destekleyici yönde idi. MTT sonuçlarına göre grup 3 ve 4’te hücre canlılık oranlarının azaldığı görüldü. Alternatif tıpta tedavi edici ve koruyucu olarak kullanılan Panax ginseng'in çalışmamızda, Sisplatin tedavisinden önce kullanılmasının YKG1 hücrelerinde Bax/Bcl 2 oranını değiştirmediği fakat Sisplatinle birlikte Caspase-3 aktivitesini arttırarak apoptozu tetikleyebileceği düşünüldü.

Anahtar Kelimeler

• Bax/Bcl
• Caspase-3
• Glioblastoma
• Panax ginseng

Kaynakça

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