Molecular Prevalence and Phylogenetic Characterization of Blastocystis in Cattle in Kayseri Province, Turkey

Abstract

Blastocystis is one of the most common emerging zoonotic parasites in humans and animals. This study aimed to determine the molecular prevalence and subtype of Blastocystis in cattle (Bos taurus). A total of 150 fresh fecal samples were collected from slaughtered cattle from various slaughterhouses in Kayseri Province, Central Anatolia. Genomic DNA was extracted from all samples and used in PCR analyses of the small subunit ribosomal RNA (SSU rRNA) gene of Blastocystis. Blastocystis positive samples were sequenced for identify subtypes. Obtained sequences were assembled with suitable genetic software, then phylogenetic relationships were revealed. According to PCR analyses, overall prevalence of Blastocystis was determined as 58.7%. The sequence analyses of the PCR product gene revealed the presence of one known livestock-specific subtype, ST10. Phylogenetic analysis revealed that ST10 isolates the characterized in the study were clustered with isolates identified previously from cattle. Molecular characterization and subtype of Blastocystis sp. in slaughtered cattle in slaughterhouses were obtained data with this study.

Keywords

• Blastocystis
• Cattle
• Phylogenetic Characterization
• Subtype
• Molecular Prevalence

Kayseri Yöresinde Sığırlarda Blastocystis’in Moleküler Prevalansı ve Filogenetik Karakterizasyonu

Öz

Blastocystis insanlarda ve hayvanlarda en yaygın bulunan zoonotik parazitlerden biridir. Bu çalışmada, sığırlarda (Bos taurus) Blastocystis’in moleküler prevalansı ve alt tiplerinin belirlenmesi amaçlanmıştır. Kayseri yöresinde bulunan çeşitli kesimhanelerde kesilen toplam 150 sığırdan taze dışkı örnekleri toplanmıştır. Dışkı örneklerinden genomik DNA ekstraksiyonu yapılmış ve DNA örnekleri Blastocystis’in small subunit ribosomal RNA (SSU rRNA) geninin amplifikasyonunda kullanılmıştır. Tüm PCR pozitif ürünler alt tiplerin belirlenmesi için sekanslanmıştır. Elde edilen sekanslar uygun genetik yazılımlarla işlenerek genotipik yapıları ve sonrasında da filogenetik ilişkileri ortaya çıkarılmıştır. İncelemesi yapılan örneklerin SSU rRNA geninin PCR analizleri ile Blastocystis’in ortalama prevalansı %58,7 olarak belirlenmiştir. Blastocystis SSU rRNA geninin PCR ürünlerinin sekans analizleri sonucunda izolatların ruminant spesifik alt tip, ST10, içerisinde olduğu tespit edilmiştir. Filogenetik analizler çalışmada karakterize edilen ST10 izolatlarının daha önce sığırlarda identifiye edilen izolatlarla birlikte kümelendiğini ortaya koymuştur. Bu çalışma ile mezbahada kesilen sığırlarda Blastocystis sp.’nin moleküler karakterizasyonu ve alt tipi üzerine veriler elde edilmiştir.

Anahtar Kelimeler

• Alt tip
• Blastocystis
• Filogenetik Karakterizasyon
• Moleküler Prevalans
• Sığır

Kaynakça

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