Effects of Vitamin C on Male New Zealand White Rabbits Exposed to Endosulfan

Yıl 2018, Cilt: 11 Sayı: 2, 165 - 172, 18.04.2018

Özlem Yıldız_gülay Tülay Büyükoğlu Fatma Şefika Hatipoğlu Mehmet Şükrü Gülay

https://doi.org/10.30607/kvj.394088

Öz

The present study evaluated the protective
effect of oral Vitamin C (VitC) against changes in hematological and
biochemical parameters, and oxidant-antioxidant status in male New Zealand
White rabbits treated with endosulfan. Total of 24 rabbits (6 to 8 years old)
were randomly divided into 4 treatment groups (n=6). Rabbits in the control
group (TRT-I) received corn oil for 6 weeks. Rabbits in the second group
(TRT-II) received endosulfan (1 mg/kg bw per day) in corn oil. Rabbits in the
third group (TRT-III) received corn oil daily and VitC (20 mg/ kg bw) every
other day for 6 weeks. Rabbits in the last group (TRT-IV) received the same
amounts of endosulfan and VitC. All treatments were administered by oral route.
Although total erythrocyte, trombocyte, hemoglobin and percent hematocrit,
lymphocyte, granulocyte and monocyte did not differ among the treatment groups,
total leukocyte numbers differed due to treatment. The serum glucose, alanine
aminotransferase (ALT), malondialdehyde (MDA), glucose 6 phosphate
dehydrogenase (G6PD), glutathion peroxidase (GPx), and erythrocyte catalase
(CAT) in treatment groups were similar. However, serum VitC levels were
elevated for rabbits in TRT-II  group. In
addition, serum alkaline phosphatase (ALP) and aspartate aminotransferase (AST)
levels increased in the TRT-II group, whereas VitC application decreased ALP
and AST levels in the TRT-IV group when compared to the TRT-II group. Thus,
results suggested beneficial influences of VitC in neutralizing some harmful
effects of endosulfan in male New Zealand White rabbits.

Anahtar Kelimeler

blood parameters, biochemical parameters, oxidant-antioxidant status, endosulfan toxication, insecticide use, Vitamin C

Endosülfan ve C Vitamini Uygulamalarının Erkek Yeni Zellanda Tavşanları Üzerindeki Etkisi

Öz

Çalışmamızda
endosülfan uygulaması yapılan erkek Yeni Zellanda tavşanlarında C vitamini
(VitC) ilavesinin bazı kan ve biyokimyasal parametreler ile oksidan-antioksidan
denge üzerine düzeltici etkisinin araştırılması amaçlanmıştır. Bu amaçla,
yaşları 6 ile 8 ay arasında değişen 24 tavşan, her grupta 6 tavşan olacak
şekilde, rastgele 4 çalışma grubuna ayrıldı. Kontrol grubundaki tavşanlara
(TRT-I) mısır yağı verildi. İkinci grupta bulunan tavşanlara (TRT-II) günlük 1
mg/kg endosülfan mısır yağında eritilerek verildi. Üçüncü gruptaki
tavşanlara  20 mg/kg dozunda VitC gün
aşırı olarak uygulandı (TRT-III). Son gruptaki tavşanlara (TRT-IV) aynı
oranlarda endosülfan ve VitC verildi. Oral yolla yapılan bu uygulamalar 6 hafta
boyunca devam etti. Çalışmamızda toplam eritrosit ve trombosit, hemoglobin,
hematokrit, ve lökosit değerleri yönünden gruplar arasında fark gözlenmezken,
toplam lökosit sayılarında anlamlı bir fark bulundu. Ayrıca serum glukoz,
alanin aminotransferaz (ALT), malondialdehit (MDA), glukoz 6 fosfat
dehidrojenaz (G6PD), glutatyon peroksidaz (GPx) ve eritrosit katalaz (CAT)  aktivitelerinde de farklılık tespit edilmedi.
TRT-III grubunda serum VitC seviyesi yükselirken, TRT-II grubunda serum alkalen
fosfataz (ALP) ve aspartat aminotransferaz (AST) seviyeleri arttı. Bununla
birlikte VitC uygulamasının TRT-IV grubundaki tavşanlarda ALP ve AST
seviyelerini TRT-II grubuna göre azalttığı gözlendi. Elde edilen bulgulara
göre, VitC uygulamasının endosülfanın bazı zararlı etkilerine karşı erkek Yeni
Zellanda tavşanlarında koruyucu olabileceği sonucuna varıldı. 

Anahtar Kelimeler

kan parametreleri, biyokimyasal parametreler, oksidan-antioksidan denge, endosülfan toksikasyonu, insektisit kullanımı, Vitamin C

Kaynakça

• Aebi H. Catalase in vitro. Methods Enzymol. 1984; 105, 121-126.
• Akay MT, Ozmen G, Elcüman EA. Effects of combinations of endosulfan, dimethoate ve carbaryl on immune ve hematological parameters of rats. Vet Hum Toxicol. 1999; Oct;41(5):296-9.
• Albanese AA, Wein EH, Mata LA. An improved method for determination of leukocyte and plasma ascorbic acid of man with applications to studies on nutritional needs and effects of cigarette smoking. Nutr Rep Internat. 1975; 12: 271-289.
• Bebe, F. N., Panemangalore M. (2003). Exposure to low doses of endosulfan and chlorpyrifos modifies endogenous antioxidants in tissues of rats. J. Environ. Sci. Health B B 2003; 38: 349– 363.
• Beutler E. Glucose-6 Phosphate Dehydrogenase Deficiecy. S. In: The Metabolic Bases of the Inheredited Disease. New York. 1983; 1629-1653.
• Blanco-Coronado JL, Repetto M, Ginestal RJ, Vicente JR, Yelamos F, Lardelli A. Acute intoxication by endosulfan. J Toxicol Clin Toxicol. 1992; 30(4): 575-583.
• Bremer JN, Leist KH. Endosulfan (AE F002671, substance technical). Valuation of the acute oral ve dermal toxicity. Hoechst document A59823, Frankfurt, Germany, 1998.
• Bucher U. Labormethoden in der Haematologie. Verlag Hans Huber, Bern. 1988.
• Daidoji T, Inoue H., Kat S, Yokota H. 2003. “Glucuronidation and excretion of nonylphenol in perfused rat liver”, Drug Metabolism and Disposal. 2003; 31: 993-998.
• Daniel CS, Agarwal S, Agarwall SS. Human red blood cell membrane damage by endosulfan. Toxicol Lett. 1986; 32 (1-2), 113-118.
• Dawson EB, Harris WA, Powell LC. Relationship between ascorbic acid and male fertility. World Rev. Nutr. Diet. 1990; 62: 1–26.
• Dawson EB, Harris WA, Teter MC, Powell LC. Effect of ascorbic acid supplementation on the sperm quality of smokers. Fertil. Steril. 1992; 58: 1034–1039.
• Dorval J, Leblond VS, Hontela A. Oxidative stress and loss of cortisol secretion in adrenocortical cells of rainbow trout (Oncorynchus mykiss) exposed in vitro to endosulfan, an organochlorin pesticide. Aquat Toxicol. 2003; 8; 63 (3): 229-241.
• Feteris WA. A serum glucose method without protein precipitation. Am J Med Technol. 1965; 31: 17-21.
• Hatipoglu FS, Gulay MS, Balic A, Yildiz-Gulay O, Volkan S. Subacute Oral Toxicity of Endosulfan in Male New Zealand White Rabbits. Toxicological Mechanism Method. 2008; 18: 705-710.
• Henderson AR, Moss DW. Tietz fundamentals of clinical chemistry. Editörler: Burtis, C.A., Ashwood, E.R., Lubbok, Texas. 2005; 352-392.
• Kannan K., Jain SK. Oxygen radical generation and endosulfan toxicity in Jurkat T-cells. Mol. Cell. Biochem. 2003; 247: 1–7.
• Kiran R, MN. Varma. Biochemical studies on endosulfan toxicity in different age groups of rats, Toxicol. Lett. 1988; 44: 247–252.
• Korkmaz A, Ahbab MA, Kolankaya D, Barlas N. “Influence of vitamin C on bisphenol A, nonylphenol and octylphenol induced oxidative damages in liver of male rats”, Food and Chemical Toxicology. 2010; 48(10): 2865-2871.
• Kumar N, Ambasankar K, Krishnani KK., Gupta SK., Bhushan S, Minhas SP. Acute toxicity, biochemical and histopathological responses of endosulfan in Chanos chanos. Ecotoxicol Environ Saf. 2016; 131: 79-88.
• Mansour S, Abdel-Mageed M, Mohamed K, Gad M, Gamet-Payrastre L. Adverse Effects to Suckling Mice Following Indirect Exposure to a Pesticide Mixture and Ameliorative Effect of α-Tocopherol Coadministration. J Basic Clin Health Sci. 2017; 3: 71-78.
• May MJ. Is ascorbic acid an antioxidant for the plasma membrane? FASEB J. 1999; 13: 995-1006.
• Menezes RG, Qadir TB, Moin A, Fatima H, Hussain SA, Madadin M, Pasha SB, Al Rubaish FA, Senthilkumaran S. Endosulfan poisoning: An overviewEndosulfan poisoning: An overview. J Foren Legal Med. 2017; 51: 27–33.
• Naqvi SM, Vaishnavi C. Bioaccumulativ potential and toxicity of endosulfan insecticide to nontarget animals Comp Biochem Physiol C. 1993; 105(3):347-361.
• Padh H. Cellular functions of ascorbic acid. Biochem. Cell Biol. 1990; 68: 1166-1173
• Pal AK, Jadhao SB, Garg UK., Jha GJ. Haemato-biochemical ve immuno-pathophysiological effects of chronic toxicity with synthetic pyrethroid, organophosphate ve chlorinated pesticides in broiler chicks. International immunopharmacology. 2004; 13(4): 1709-1722.
• Pistl J, Kovalkovicova N, Holovska VV, Legath J, Mikula I. (2003) Determination of the immunotoxic potential of pesticides on functional activity of sheep leukocytes in vitro. Toxicology 3, 188 (1):73-81.
• Rao M, Narayana K, Benjamin S, Bairy KL. L-Ascorbic acid ameliorates postnatal endosulfan induced testicular damage in rats. Indian J. Physiol. Parmacol. 2005; 49: 331–36.
• Satoh K. Serum lipid peroxide in cerebrovascular disorders determined by a new colorimetric method. Clin Chim Acta. 1978; 90: 37-43.
• Segasothy M, Pang KS. Acute interstitial nephritis due to endosulfan. Nephron. 1992; 62: 118.
• Sharma S, Dewan A, Singh G. Toxico-vigilance: an inevitable prerequisite to keep a watch on toxins around you. J Forensic Leg Med, 2017; 45: 32–35.
• Simonich SL, Hites RA. Global distribution of persistent organochlorine compounds. Science. 1995; 29:1851–1854.
• Singh PP, Kumar A, Chauhan RS, Pankaj PK. Effect of endosulfan on immunological competence of layer birds. Veterinary World. 2016; 9: 777-782.
• Sohn H, Kwon C, Kwon G, Lee J, Kim E. Induction of oxidative stress by endosulfan and protective effect of lipid-soluble antioxidants against endosulfan induced oxidative damage. Toxicol. Lett. 2004; 151: 357–65.
• Xu D, Li S, Lin L, Qi F, Hang X, Sun Y. Gene expression profiling to identify the toxicities and potentially relevant disease outcomes due to endosulfan exposure. Toxicol Res. 2016; 5: 621-632.
• Xu D, Liu T, Lin L, Li S, Hang X, Sun Y. Exposure to endosulfan increases endothelial permeability by transcellular and paracellular pathways in relation to cardiovascular diseases. Environ Pollut. 2017; 223: 111–119.
• Videla LA. “Oxidative stress signaling underlying liver disease and hepatoprotective mechanisms”,World J. Hepatology. 2009; 1: 72-78.
• Zhang P, Zhu W, Wang D, Yan J, Wang Y, Zhou Z, He L. A combined NMR- and HPLC-MS/MS-based metabolomics to evaluate the metabolic perturbations and subacute toxic effects of endosulfan on mice. Environ Sci Pollut Res. 2017; 24: 18870-18880.