Investigation of Cytotoxic Effects of Curcuma Longa, Zingiberaceae and Dianthus Caryophllus, Which are Commonly Used as Food Supplements in Daily Life

Yıl 2019, Cilt: 12 Sayı: 3, 351 - 356, 30.09.2019

Özgür Albuz

https://doi.org/10.30607/kvj.593514

Cited By: 1

Öz

The aim of this study
is to investigate the cytotoxic effects, which have an important place ,in
terms of public health as using herbal food supplement, of ginger, turmeric and
clove seeds over healthy cells. In this study, designed as an in vitro cell
culture study, fibroblast cells were used to investigate the effect of plant
extracts on healthy cells. MTT cell viability test was used to determine
cytotoxicity. The MTT test was performed according to ISO 10993-5 standards and
when the cell viability was calculated to be below 70% in the MTT method, the
sample was considered potential cytotoxic. When the viability values calculated
according to the absorbance values obtained from MTT test are examined; It was
calculated that ginger and turmeric extracts were not cytotoxic at any
concentration, including the highest concentrations. On the other hand, it was
calculated that 1: 2 concentration of clove extract was cytotoxic and the
viability was 26.7%. As a result of this study, it has been found that clove
use is remarkable in terms of plant nutritional supplements with its current
use in terms of cytotoxic effect compared to turmeric and ginger. 

Anahtar Kelimeler

Curcuma Longa, Zingiberaceae, Dianthus Caryophllus, Cytoxicity

Günlük Yaşamda Gıda Takviyesi Olarak Kullanılan Zencefil, Zerdeçal ve Karanfilin Sitotoksik Etkilerinin Araştırılması

Öz

Bu çalışmanın amacı,
günlük yaşamda gıda takviyesi olarak kullanılan ve halk sağlığı açısından
önemli bir yeri olan zencefil, zerdeçal ve karanfil tohumlarının sağlıklı
hücreler üzerinde sitotoksik etkilerini araştırmaktır. İn vitro hücre kültürü
çalışması olarak tasarlanan bu çalışmada, bitki özlerinin sağlıklı hücreler
üzerindeki etkisini araştırmak için fibroblast hücreleri kullanılmıştır.
Sitotoksisiteyi belirlemek için MTT hücre canlılığı testi kullanılmıştır. MTT
testi ISO 10993-5 standartlarına göre yapıldı ve MTT yönteminde hücre canlılığı
% 70'in altında olduğu hesaplandığında, numunenin sitotoksik potansiyelde
olduğu kabul edilmiştir. MTT
testinden elde edilen absorbans değerlerine göre hesaplanan canlılık değerleri
incelendiğinde; Zencefil ve zerdeçal ekstrelerinin, en yüksek konsantrasyonlar
dahil olmak üzere herhangi bir konsantrasyonda sitotoksik olmadığı
hesaplanmıştır. Öte yandan, 1:2 oranında karanfil ekstresi konsantrasyonunun
sitotoksik ve canlılığın % 26.7 olduğu hesaplanmıştır. Bu çalışma sonucunda, özellikle karanfil kullanımının zerdeçal ve
zencefil ile karşılaştırıldığında sitotoksik etkiye sahip olması bakımından
mevcut kullanımıyla, bitkisel gıda takviyeleri açısından dikkate değer olduğu
tespit edilmiştir.

Anahtar Kelimeler

Zerdeçal, zencefil, karanfil, sitotoksitite

Kaynakça

• Ahmed K, Shaheen G, Asif HM. Zingiber officinale Roscoe (pharmacological activity). J. Med. Plants Res.. 2011: 5(3), 344-348.
• Aggarwal BB, Sundaram C, Malani N, Ichikawa H. Curcumin: the Indian solid gold. In: The molecular targets and therapeutic uses of curcumin in health and disease. Springer, Boston, MA. 2007; pp. 1-75.
• Akram M, Shahab-Uddin AA, Usmanghani K, Hannan A, Mohiuddin E, Asif M. (2010). Curcuma longa and curcumin: a review article. Rom J Biol Plant Biol. 2010: 55(2), 65-70.
• Al-Snafi AE. Chemical contents and medical importance of Dianthus caryophyllus-A review. IOSR Journal of Pharmacy. 2017: 7(3), 61-71.
• Balaji S, Chempakam B. Toxicity prediction of compounds from turmeric (Curcuma longa L). Food Chem Toxicol.2010: 48(10), 2951-2959.
• Chattopadhyay I, Biswas K, Bandyopadhyay U, Banerjee RK. Turmeric and curcumin: Biological actions and medicinal applications. Curr Sci. 2004: 87, 44-53.
• Elmadfa I, Meyer AL. Importance of food composition data to nutrition and public health. Eur J Clin Nutr. 2010: 64(S3),4-7.
• Fern K. Plants for a future: Edible & Useful plants for a healthier world. Permanent publications. Hampshire, UK. 1997.
• Gevrenova R, Joubert O, Mandova T, Zaiou M, Chapleur Y, Henry M. Cytotoxic effects of four Caryophyllaceae species extracts on macrophage cell lines. Pharm biology. 2014: 52(7), 919-925.
• Gilbert DF, Boutros M. A protocol for a high-throughput multiplex cell viability assay. In: High-Throughput RNAi Screening. Humana Press, New York, NY.2016. pp. 75-84
• Gopal J, Anthonydhason V, Muthu M, Gansukh E, Jung S, Chul S, Iyyakkannu S. Authenticating apple cider vinegar’s home remedy claims: antibacterial, antifungal, antiviral properties and cytotoxicity aspect. Nat Prod Res. 2019: 33(6), 906-910.
• Haniadka R, Saldanha E, Sunita V, Palatty PL, Fayad R, Baliga MS. A review of the gastroprotective effects of ginger (Zingiber officinale Roscoe). Food & Function. 2013: 4(6), 845-855.
• Jolad SD, Lantz RC, Solyom AM, Chen GJ, Bates RB, Timmermann BN. Fresh organically grown ginger (Zingiber officinale): composition and effects on LPS-induced PGE2 production. Phytochemistry. 2004: 65(13), 1937-1954.
• Mahavorasirikul W, Viyanant V, Chaijaroenkul W, Itharat A, Na-Bangchang K. Cytotoxic activity of Thai medicinal plants against human cholangiocarcinoma, laryngeal and hepatocarcinoma cells in vitro. BMC Complement Altern Med. 2010: 10(1), 55-62.
• Mohammed MJ, Al-Bayati FA. Isolation and identification of antibacterial compounds from Thymus kotschyanus aerial parts and Dianthus caryophyllus flower buds. Phytomedicine. 2009: 16(6-7), 632-637.
• Park EJ, Pezzuto JM. Botanicals in cancer chemoprevention. Cancer and Metastasis Reviews. 2002: 21(3-4), 231-255.
• Plengsuriyakarn T, Viyanant V, Eursitthichai V, Tesana S, Chaijaroenkul W, Itharat A, Na-Bangchang, K. Cytotoxicity, toxicity, and anticancer activity of Zingiber officinale Roscoe against cholangiocarcinoma. Asian Pac J Cancer. 2012: 13(9), 4597-4606.
• Polunin O, Huxley A, Everard B. Flowers of the Mediterranean. Chatto and Windus. London.1965. pp. 161.
• Riss TL, Moravec RA. Use of multiple assay endpoints to investigate the effects of incubation time, dose of toxin, and plating density in cell-based cytotoxicity assays. Assay Drug Dev Technol. 2004: 2(1), 51-62.
• Shukla Y, Singh M. Cancer preventive properties of ginger: a brief review. Food Chem Toxicol. 2007: 45(5), 683-690.
• Tokur O, Aksoy A. In vitro sitotoksisite testleri. Harran Üniv Vet Fak Derg. 2017: 6(1), 112-118.
• Toptaş B, Alagöz Z.A. Anticarcinogenic Effects of Curcumin and It’s Analogs. Ankara Ecz Fak Derg. 2016: 40(2), 58-82.
• Tyler VE, Foster S. The honest herbal: a sensible guide to the use of herbs and related remedies (Vol. 37). New York: Pharmaceutical Products Press. 1993. pp: 375.
• Zhu JS, Halpern GM, Jones K. The scientific rediscovery of an ancient Chinese herbal medicine: Cordyceps sinensis Part I. The Journal of alternative and complementary medicine. 1998: 4(3), 289-303.
• Zucco F, De Angelis I, Stammati A. (1998). Cellular models for in vitro toxicity testing. In: Animal Cell Culture Techniques. Springer, Berlin, Heidelberg. 1998. pp. 395-422