Farklı Üreme Yaşındaki Keçilerde Dondurulmuş Sperma ile Servikal Tohumlama

Yıl 2019, Cilt: 12 Sayı: 3, 357 - 362, 30.09.2019

Koray Tekin

https://doi.org/10.30607/kvj.604959

Öz

Keçide dondurulmuş sperma ile servikal suni
tohumlama (ST) uygulaması düşük gebelik oranları nedeniyle halen sınırlıdır. Bu
çalışmanın amacı iki farklı üreme yaşındaki Saanen keçilerinde donmuş sperma
kullanımının etkinliğini belirlemekti. Senkronizasyon ve gebelik oranlarının
etkileri de incelenmiştir. Doksan sekiz (98) dişi Saanen keçisi iki gruba
ayrıldı: Grup-1 = 49 (7 aylık) ve Grup-2 = 49 (19 aylık) keçi. Östrus 11 gün süresince
intravajinal sünger (30 mg;  fluogestone
acetate, FGA)
ve süngerin çıkarılmasından 48 saat önce 50 ug kloprostenol (PGF2a) ve gebe
kısrak serum gonadotropini (PMSG, 500 IU) ile senkronize edildi. Sabit zamanlı
servikal tohumlama dondurulmuş sperma ile üreme mevsiminde süngerlerinin
çıkarılmasından 43-45 saat sonra gerçekleştirildi. Östrus arama tekesi ile
tespit edildi. Grup 1 ve Grup 2'de östrus görülme oranları sırasıyla %71.4,
%63.3 olarak tespit edildi (p>0.05). Altmışaltı (66) keçi dondurulmuş-çözdürülmüş
spermayla tohumlandı. Gebelik abdominal ultrasonografi ile belirlendi. Gebelik
oranları (Grup-1: %31.4, Grup-2: %32.3) yaş grupları arasında anlamlı bir
farklılık göstermedi (p>0.05). Ayrıca, artan çoklu doğum ve azalan
embriyonik kayıplar sayesinde Grup-1’de (%145.5), Grup-2’ye (%90.0) göre daha
yüksek oranda yavru elde edilmiştir. Sonuç olarak, Saanen keçileri, 7 aylık
yaştan itibaren suni tohumlama programlarına alınabilir ve yüksek
senkronizasyon başarısı ve yavru verimine gibi birçok fayda sağlayabilir.

Anahtar Kelimeler

Dondurulmuş sperma, fertilite, gebelik, üreme yaşı, suni tohumlama

Cervical Insemination with Frozen Thawed Semen in Goats at Different Breeding Age

Öz

Artificial insemination (AI) in goat is currently
limited by the poor fertility obtained following the cervical insemination with
frozen-thawed semen. The objective of the present study was to determine the
efficiency of frozen semen use in two different breeding ages at Saanen goats.
The effects of synchronization and fertility rates were also examined.
Ninety-eight (98) Saanen goats were divided into two groups: Group-1 = 49
(7-months-old) and Group-2 = 49 (19-month- old) does. Estrus was
synchronized by impregnated intravaginal sponges (30 mg; fluogestone
acetate,
FGA), and i.m. administration of 50 µg of cloprostenol (PGF2α) and
pregnant mare serum gonadotropin (PMSG, 500 IU), at 48 h before
sponge removal. Cervical fixed time AI was carried out with frozen semen
43 h after removal of the sponges during the breeding season. Estrus was
detected by teaser buck. The occurrence of estrus in Group-1 and Group-2 were
71.4%, 63.3%, respectively (p>0.05). Sixty-six (66) goats were inseminated
with frozen‐thawed semen. The pregnancy rate was determined by
abdominal scanning. Pregnancy rates were (Group-1: 31.4%, Group-2: 32.3%) not
differed significantly between age groups (p>0.05). Besides, higher lambing
rates were obtained in Group-1 (145.5%) than in Group-2 (90.0%) due to
increased prolificacy and reduced embryonic failure. In conclusion, Saanen goats in younger ages, starting
from the 7 months old age could be included in the breeding programs and could
provide several benefits such as higher percentages of synchronization success
and prolificacy. 

Anahtar Kelimeler

Artificial Insemination, breeding age, frozen semen, pregnanc, fertility

Kaynakça

• Althouse G. Artificial Insemination In: Comparative reproductive biology, Ed; Schatten H, Constantinescu GM, Ames IO, Blackwell. 2007; pp. 159-170.
• Andries KM. Using Performance Ratios and EBVs for Selection in Meat Goats. In: Agricultural Research and Extension Program Langston University, Proceedings of the 32nd Annual Goat Field Day, April 29, Oklahoma, USA. 2017.
• Anel LM, Kaabi B, Abroug M, Alvarez E, Anel JC, Boixo LF. Factors influencing the success of vaginal and laparoscopic artificial insemination in Churra ewes: a field assay. Theriogenology. 2005; 63: 1235-1247.
• Arrebola FA, Pardo B, Sánchez M, López MD, Pérez-Marín CC. Factors influencing the success of an artificial insemination program in Florida goats. Spanish Journal of Agricultural Research. 2012; 10(2): 338-344.
• Bolet G. Timing and extent of embryonic mortality in pigs, sheep and goats: Genetic Variability. In: Embryonic mortality in farm animals, Ed; Sreenan JM, Diskin M, Springer Dordrecht. 1986; pp. 12-43.
• Cognie Y, Baril G, Poulin N, Mermillod P. Current status of embryo technologies in sheep and goats. Theriogenology. 2003; 59: 171–188.
• Diskin MG, Morris DG. Embryonic and early foetal losses in cattle and other ruminants. Reprod Domest Anim. 2008; 43(2): 260-267.
• Donovan A, Hanrahan JP, Kummen E, Duffy P, Boland MP. Fertility of the ewe following cervical insemination with fresh or frozen-thawed semen at natural or synchronised oestrus. Animal Reproduction Science. 2004; 84: 359-368.
• Donovan A, Hanrahan JP, Lally T, Boland MP, Byrne GP, Duffy P, O'Neill DJ. AI for sheep using frozen-thawed semen. ARMIS 4047 Project report under the Research Stimulus Fund; OPARDF measure 5b. 2001.
• Ehling C, Wirth P, Schindler L, Hadeler KG, Döpke HH, Lemme E, Niemann H. Laparoscopical intrauterine insemination with different doses of fresh conserved and frozen-thawed semen for the production of ovine zygotes. Theriogenology. 2003; 60: 777-787.
• Fernandez Abella D, Preve MO, Villegas N. Insemination time and diluting rate of cooled and chilled ram semen affects fertility. Theriogenology. 2003; 60: 21-26.
• Hill JR, Thompson JA, Perkins NR. Factors affecting pregnancy rates following laparoscopic insemination of 28447 Merino ewes under commercial conditions: a survey. Theriogenology. 1998; 49: 697-709.
• Hyacinth AA, Terzungwe A, Daniel OL, Oluwabamise NJ. Biometrical Study of the Genitalia of Kano Brown Goats and Yankasa Sheep. IJAVMS. 2016; 10(1): 3-8.
• Intrakamhaeng M, Kongbuntad W, Wangkahart E, Khalid M, Leethongdee S. The anatomy of cervix and the cervical penetrability during oestrus in the Thai goat. Reproduction in Domestic Animals. 2011; 46: 113.
• Karagiannidis A, Varsakeli S, Karatzas G, Brozos C. Effect of time of artificial insemination on fertility of progestegen and PMSG treated indigenous Greek ewes during non-breeding season. Small Ruminant Research. 2001; 39(1): 67-71.
• Karatzas G, Karagiannidis A, Varsakeli S, Brikas P. Fertility of fresh and frozen-thawed goat semen during the nonbreeding season. Theriogenology. 1997; 48(6): 1049-1059.
• King ME, McKelvey WAC, Dingwall WS, Matthews KP, Gebbie FE, Mylne MJA, Robinson JJ. Lambing rates and litter sizes following intrauterine or cervical insemination of frozen-thawed semen with or without oxytocin administration. Theriogenology. 2004; 62: 1236-1244.
• Kukovics S, Gyökér E, Németh T, Gergátz E. Artificial insemination of sheep–possibilities realities and techniques at the farm level. In: Artificial insemination in farm animals. Ed; Manafi M, InTech Crotia. 2011.
• Kulaksiz R, Daskin A. Reproductive performance of primiparous and multiparous Saanen goats after laparoscopic intrauterine insemination: a field study. Turkish Journal of Veterinary and Animal Sciences. 2012; 36(2): 201-204.
• Leboeuf B, Delgadillo JA, Manfredi E, Piacère A, Clément V, Martin P, De Cremoux R. Management of goat reproduction and insemination for genetic improvement in France. Reproduction in Domestic Animals. 2008; 43: 379-385.
• Leboeuf B, Manfredi E, Boue P, Piacère A, Brice G, Baril G, Terqui M. Artificial insemination of dairy goats in France. Livestock Production Science. 1998; 55(3): 193-203.
• Leboeuf B, Restall B, Salamon S. Production and storage of goat semen for artificial insemination. Animal Reproduction Science. 2000; 62(1-3): 113-141.Leethongdee S, Ponglowhapan S. Artificial insemination in goats: an update. Thaj J Vet Med. 2014; 44(1): 73-77.
• Mellado M, Olivas R, Ruiz F. Effect of buck stimulus on mature and pre-pubertal norgestomet-treated goats. Small Ruminant Research. 2000; 36(3): 269-274.
• Moses D, Martinez AG, Iorio G, Valcarcel A, Ham A, Pessi H, De Las Heras MAA. Large-scale program in laparoscopic intrauterine insemination with frozen-thawed semen in Australian Merino sheep in Argentine Patagonia. Theriogeneology. 1997; 48: 651-657.
• Motlomelo KC, Greyling JPC, Schwalbach LMJ. Synchronisation of oestrus in goats: the use of different progestagen treatments. Small Ruminant Research. 2002; 45(1): 45-49.
• Notter DR. Effects of ewe age and season of lambing on prolificacy in US Targhee Suffolk and Polypay sheep. Small Ruminant Research. 2000; 38(1): 1-7.
• Perkins NR, Hill JR, Pedrana RG. Laparoscopic insemination of frozen-thawed semen into one or both uterine horns without regard to ovulation site in synchronised Merino ewes. Theriogenology. 1996; 46: 541-545.
• Purdy PH, Blackburn HD, Larson B, Stobart R. Investigation of a Novel Non-Surgical Method of Artificial Insemination for Sheep. Publications from USDA-ARS / UNL Faculty.1395. 2009: http://digitalcommons.unl.edu/usdaarsfacpub/1395
• Romano JE, Crabo BG, Christians CJ. Effect of sterile service on estrus duration fertility and prolificacy in artificially inseminated dairy goats. Theriogenology. 2000; 53(6): 1345-1353.
• Salamon S, Maxwell WM. Frozen storage of ram semen II. Causes of low fertility after cervical insemination and methods of improvement. Animal Reproduction Science. 1995; 38(1-2): 1–36.
• Samir H. Karen A. Ashmawy T. Abo-Ahmed M. El-Sayed M. Watanabe G. Monitoring of Embryonic/Fetal Losses in Different Breeds of Goats using Real Time B- Mode Ultrasonography. Theriogenology. 2016; 85(2): 207-215.
• Sanwal PC, Pande JK, Varshney VP, Mogha IV. Gonadotrophin-induced ovulation in melengestrol acetate treated female goat. Indian Journal of Physiology And Pharmacology. 1983; 27(1): 57-60.
• Szabados T. A cervikouterinális inszeminálás eredményességének vizsgálata juhászatokban /Study of effectiveness in cervico-uterinal insemination of ewes. PhD thesis, University of Western Hungary Faculty of Agriculture and Food Sciences, Mosonmagyaróvár Hungary, 2006.
• Tirpan MB, Tekin K, Cil B, Alemdar H, Inanc ME, Olgac KT, Stelletta C, Daskin A. The effects of different PMSG doses on estrus behavior and pregnancy rate in Angora goats. Animal. 2019; 13(3): 564-569.
• Vanroose G, de Kruif A, Van Soom A. Embryonic mortality and embryo–pathogen interactions. Animal Reproduction Science. 2000; 60: 131-143.
• Wentzel D. Non-infectious abortion in Angora goats. 3rd Intern conference on goat production and disease. Tuscon, 10–15 Jan. 1982; pp: 155–161. Dairy goat Journal publishing Co. Scottsdale USA. 1982; pp: 155–161.
• Whitley NC, Jackson DJ. An update on estrus synchronization in goats: A minor species Journal of Animal Science. 2004; 82(13): E270–276.
• Yamaki K, Morisawa M, Ribadulla A, Kojima J. Sheep semen characteristics and artificial insemination by laparoscopy. Tohoku Journal of Agricultural Research. 2003; 54(1-2): 17-26.
• Yeni D, Gündoğan M. Some andrological parameters and biochemical properties in relation to season in rams. Kocatepe Veterinary Journal. 2018; 11(1): 70-85.
• Yotov SA. Ultrasound diagnostics of late embryonic and foetal death in three sheep breeds. Journal of Veterinary Advances. 2012; 2(3): 120-125.
• Yotov SA, Velislavova DV, Dimova LR. Pregnancy rate in Bulgarian White milk goats with natural and synchronized estrus after artificial insemination by frozen semen during breeding season. Asian Pacific Journal of Reproduction. 2016; 5(2): 144-147.