Streptozotosin ile Diyabet Oluşturulan Ratlarda Beyaz Dut Ekstraktının Performans, Antienflamatuar ve Serum Antioksidan Parametreleri Üzerine Etkisi

Yıl 2020, Cilt: 13 Sayı: 1, 1 - 10, 31.03.2020

Zekeriya Safa İnanç , Harun Karaca , Volkan İpek , Burcu Menekşe Balkan Orhan Çorum , Şükrü Güngör , Muhammed İnanç , Halit İmik , Ayhan Ata

https://doi.org/10.30607/kvj.643431

Cited By: 2

Öz

Bu çalışmada streptozotosin uygulanarak diyabet oluşturulan ratlarda
beyaz dut ekstraktının kan serumlarında antioksidan etkileri, karaciğer dokusu
histopatoloji, antienflamatuar özelliği ve ratlarda performans parametreleri
üzerine etkisi araştırıldı. Deney grupları, kontrol grubu (K, n:5),
streptozotosin uygulanan deney grubu 1 (DG 1, n:6), dut ekstraktı uygulanan
deney grubu 2 (DG 2, n:7), streptozotosin
ve dut ekstraktının birlikte uygulandığı deney grubu 3 (DG 3, n:6) olmak üzere
toplam 24 adet hayvandan oluşturuldu. Karaciğer dokusunun yapısındaki vasküler
konjesyon K ve DG 2’de 2 hayvanda, DG 3’te 1 hayvanda tespit edilmesine rağmen,
DG 1’de bütün hayvanlarda belirlenmiştir (P<0,05). Karaciğer dokusunun yangı
bulguları K’da görülmezken, DG 2’de 2 adet hayvanda hafif şekilde, DG 1’deki
tüm hayvanlarda gözlenmiştir. Karaciğer dokusundaki dejenerasyon kontrol
grubunda görülmezken, DG 2’de 1 adet, DG 1’de ise tüm hayvanlarda görülmüştür
(P<0,01). Grupların serum dokularındaki seruloplazmin düzeyleri arasında
önemli bir fark bulunmazken (P>0,05), Paraoksonaz (PON) aktiviteleri
arasındaki fark önemli düzeyde olduğu tespit edilmiştir (P<0,05). Canlı ağırlık artışı kontrol grubunda 150,03 g., DG 2 de
114,93g. olarak gerçekleşmiştir (P<0,01). DG
1’de deneme süresince 1.33 g. canlı ağırlık artışı olurken DG 3’te ağırlık
kaybı olmuştur (P>0,05). Sonuç olarak araştırmada
ratlara uygulanan dut ekstraktının
karaciğer dokusunda vasküler konjesyon, yangı ve dejenerasyon olgularını önemli
derecelerde azalttığı ve antienflamatuar etkisi gösterebileceği
söylenebilir.

Anahtar Kelimeler

Antienflamatuar , Antioksidan , Beyaz dut ekstraktı , performans , yangı

Effect of White Mulberry Extract on Performance, Anti-inflammatory and Serum Antioxidant Parameters in Diabetes-induced Rats with Streptozotocin

Öz

This study was conducted to investigate the effect of the white mulberry extract on histopathology, anti-inflammatory properties of liver tissue, blood serum antioxidant parameters and performance parameters of diabetes-induced rats with streptozotocin. Experimental groups consist of 24 animals which were divided into four groups; control group (C, n: 5), streptozotocin applied group 1 (DG 1, n: 6), mulberry extract applied group 2 (DG 2, n: 7), streptozotocin and mulberry extract experimental group 3 (DG 3, n:6), totally 24 animals were used. Although vascular congestion in the liver tissue was detected 2 animals in C and DG 2 groups and 1 animal in DG 3 group, vascular congestion was detected in all animals in DG 1 group. (P <0.05). Inflammatory findings of liver tissue were not seen in C, whereas it was mild in 2 animals in DG 2 and it was seen in all animals in the group of DG 1. Degeneration findings in liver tissue were not seen in C, but it was detected in 1 animal of DG 2 and all animals in DG 1 (P <0.01). There was no statistical difference between the ceruloplasmin levels of the groups (P> 0.05).  Live weight gain in control group 150,03 g., DG 2 114.43 g. (P <0,01). 1.33 g. live weight gain and weight loss in DG 3 (P> 0.05). In conclusion, mulberry extract applied to rats significantly reduced vascular congestion, inflammation and degeneration findings in liver tissue and it can be said that mulberry extract has antiinflammatory effect.

Anahtar Kelimeler

Anti-inflammatory , Antioxidant , İnflammation , Performance , White Mulberry Extract

Kaynakça

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