Karaciğer Trematodlu Koyunların Serum ve Yapağılarında Kobalt, Mangan, Selenyum ve Çinko Düzeylerinin Araştırılması

Yıl 2020, Cilt: 13 Sayı: 2, 185 - 191, 30.06.2020

Selçuk Seçkin Tuncer , Vural Denizhan , Süleyman Kozat

https://doi.org/10.30607/kvj.696241

Cited By: 1

Öz

Bu çalışmada sağlıklı ve karaciğer trematodu ile enfekte olmuş koyunlarda hematolojik ve biyokimyasal kan değerleri ile serum ve yün eser elementlerindeki (kobalt [Co], mangan [Mn], çinko [Zn] ve selenyum [Se]) farklılıklar karşılaştırılmıştır. Bu amaçla 80 baş karaciğer trematodlu ve 20 baş da sağlıklı olmak üzere toplam 100 baş koyun kullanıldı. Karaciğer trematodlu koyunların lökosit (WBC), nötrofil (Neu) ve eozinofil (Eo) düzeyleri kontrol grubunun aynı parametrelerinden istatistiki olarak önemli düzeyde yüksek (P<0,01) tespit edilirken, eritrosit (RBC), hematokrit (Hct) ve hemoglobin (Hb) değerleri daha düşük (P<0,01) bulunmuştur. Karaciğer trematodlu koyunların alanin aminotransferaz (ALT), aspartat aminotransferaz (AST) ve gamma glutamil transpeptidaz (GGT) değerleri sağlıklı koyunların aynı parametrelerine göre sırasıyla P<0,001, P<0,005 ve P<0,001 düzeylerinde yüksek tespit edildi. Karaciğer trematodlu koyunların serum ve yün Co, Mn, Zn ve Se seviyeleri sağlıklı koyunlara göre istatistiksel olarak önemli düzeyde düşük bulunmuştur (P<0,01). Karaciğer enfeksiyonlarının iz element konsantrasyonları üzerindeki etkisinin yün ve serumdaki ölçümlerinin benzerlik gösterdiği saptanmıştır. Karaciğer trematodlu koyunların serum ve yün iz elementlerin konsantrasyonundaki düşüşlerin karaciğerdeki patolojik bozukluklarına bağlı olarak oluşan iz elementlerin biyotransformasyonundaki değişimlerinde kaynaklanabileceği düşünülmektedir. 

Anahtar Kelimeler

Karaciğer trematodu, Serum, Koyun, İz element, Yün

Destekleyen Kurum

Van Yüzüncü Yıl Üniversitesi Bilimsel Araştırma Projeleri

Proje Numarası

FBA-2018-6823

Investigation of Serum and Wool Levels of Cobalt, Manganese, Selenium and Zinc in Liver-Trematode-Infected Sheep

Öz

In this study, the hematological and biochemical blood values and the differences in serum and wool trace elements (cobalt [Co], manganese [Mn], zinc [Zn], and selenium [Se]) were compared in healthy and liver-trematode-infected sheep. A total of 100 ovines (80 trematode-infected and 20 healthy sheep) were included. The trematode-infected sheep had significantly greater (P<0.01) leucocyte (WBC), neutrophil (Neu), and eosinophil (Eo) values and significantly lower (P<0.01) erythrocyte (RBC), hematocrit (Hct), and hemoglobin (Hb) values when compared with the healthy control group. The trematode-infected sheep had significantly higher serum alanine aminotransferase (ALT), aspartate aminotransferase (AST), and gamma glutamyl transferase (GGT) enzyme activity values when compared with the healthy control group (P<0.01, P<0.05, and P<0.01, respectively). Serum and wool Co, Mn, Zn, and Se levels were significantly lower (P<0.01) in the trematode-infected sheep than in the healthy control group. The effect of liver infections on trace element concentrations was determined to be similar in measurements in wool and serum. Decreases in trace element concentrations were mostly attributed to changes in the biotransformation of trace elements induced by pathologic disorders in the liver.

Anahtar Kelimeler

Liver-trematode, Serum, Sheep, Trace element, Wool

Proje Numarası

FBA-2018-6823

Kaynakça

• Aytuğ CN, Alaçam E, Özkoç U, Yalçın BC, Gökçen H, Türker H. Sheep - Goat diseases and breeding. Tüm Vet Hay Hizm Yay, İstanbul, Turkey. 1990; 2: 289–301.
• Babeker EA, Elmansoury YHA. Observations concerning haematological profile and certain biochemical in Sudanese Desert goat. Online J Anim Feed Res. 2010; 3(1): 80-86.
• Bayşu N, Dündar Y, Bayrak S. The relationship between copper levels in wool and blood of sheep and lambs and its diagnostic importance. Doğa Bil Derg. 1984; 8(1): 117–122.
• Beisel WR. Nutrition and infection, In; Nutritional Biochemistry and Metabolism with Clinical Application, Ed; Linder MC, 2nd Ed., Elsevier, New York, USA. 1991; pp. 507–542.
• Chalabis-Mazurek A, Walkuska G. Effect of different forms of selenium on trace elements in the blood serum and liver tissue of lambs. J Elem. 2014; 19(1): 41–53.
• Değer Y, Mert H, Mert N, Yur F, Kozat S, Yörük İH, Sel T. Serum selenium, vitamin E, and sialic acids concentrations in lambs with white muscle disease. Biol Trace Elem Res. 2008; 121(1): 39-43.
• Erdoğan S, Ergün Y, Erdoğan Z, Kontaş T. Some mineral substance levels in serum of sheep and goat grazing in Hatay Region. Turk J Vet Anim Sci. 2002; 26: 177–182.
• Gabor S. Trace elements and lipid peroxidation. Trace elements in health and disease. ÇÜ Pub Trace. 89, Adana, Turkey. pp. 273–277.
• Gerber H. Serum enzyme determination in equine medicine. Equine Vet J. 1969; 1(3): 129-139.
• Heidarpour M, Mohri M, Borji H, Moghdass E. Oxidative stress and trace elements in camel (Camelus dromedarius) with liver cystic echinococcosis. Vet Parasitol. 2012; 187(3-4): 459–463.
• Humann–Ziehank E, Ganter M, Hennig–Pauka I, Binder A. Trace mineral status and liver and blood parameters in sheep without mineral supply compared to local roe deer (Capreolus capreolus) populations. Small Rumin Res. 2008; 75(2-3): 185–191.
• Jacob RA. Trace elements, In; Textbook of Clinical Chemistry, Ed;Tietz NN and Saunders WB, Philadelphia, USA. 1987; pp. 965–978.
• Kahn CM, Line S. The Merck veterinary manual. 10th Ed., 2 nd print, Merck and Co., Whitehouse Station, Great Britain. 2011; p. 2945.
• Kaneko JJ, Harvey JW, Bruss ML. Clinical Biochemistry of Domestic Animals. 5th Ed., Academic Press. California, USA. 1997; p. 932.
• Kozat S. Importance, necessity and the effects of deficiencies of trace elements in ruminants. YYU Health Sci J. 2007; 10: 58–67.
• Kozat S, Denizhan V. Glucose, lipid, and lipoprotein levels in sheep naturally infected with Fasciola hepatica. J Parasitol. 2010; 96(3): 657–659.
• Kozat S, Ekin S, Kaya A, Denizhan V. Serum adenosine deaminase activity and selected biochemical parameters in chronic fascioliasis in sheep. Indian Vet J. 2006; 83, 249–350.
• Kurt D, Denli O, Kanay Z, Güzel C, Ceylan K. An investigation of the copper (Cu), zinc (Zn) and selenium (Se) levels of blood serum and the Cu and Zn levels of wool of Akkaraman ewes in the Diyarbakır region. Turk J Vet Anim Sci. 2001; 25(4): 431–436.
• Levieux D, Levieux A, Mage C, Venien A. Early immunodiagnosis of bovine fascioliasis using the specific antigen f2 in a passive hemagglutination test. Vet Parasitol. 1992; 44: 77–86.
• Manga-González MY, Ferreras MC, Campo R, González-Lanza C, PérezV, García-Marín JF. Hepatic marker enzymes, biochemical parameters and pathological effects in lambs experimentally infected with dicrocoelium dendriticum. Parasitol Res. 2004; 93(5): 344–55.
• Matanovic K, Severin K, Martinkovic´ F, Simpraga M, Janicki Z, Barisic J. Hematological and biochemical changes inorganically farmed sheep naturally infected with Fasciola hepatica. Parasitol Res. 2007; 101: 1657–1661.
• Mert H, Kozat S, Ekin S, Yoruk IH. Serum sialic acid, lipid-bound sialic acid levels in sheep naturally chronic infected with Fasciola hepatica. YYU Health Sci J. 2006; 2: 40–46.
• Miranda M, Alonso ML, Benedito JL. Copper, zinc, iron, and manganese accumulation in cattle from Asturias (northern Spain). Biol Trace Elem Res. 2006; 109(2): 135–143.
• NCR. Vitamin Tolerance of Animals. The National Academy Press, Washington DC, USA. 1987; p. 108.
• Oldfield EJ. The two faces of selenium. J Nutr. 1987; 117: 2002–2008.
• Phiri K, Phiri AM, Harrison LJ. The serum glucose and beta-hydroxybutyrate levels in sheep with experimental Fasciola hepatica and Fasciola gigantica infection. Vet Parasitol. 2007; 143: 287–293.
• Samadieh H, Mohammadi GR, Maleki M, Borji H, Azizzadeh M, Heidarpour M. Relationships between oxidative stress, liver, and erythrocyte injury, trace elements and parasite burden in sheep naturally infected with dicrocoelium dendriticum. Iran J Parasitol. 2017; 12(1): 46–55.
• SAS, Statistical Analysis System. SAS/STAT Software: Hangen and enhanced. SAS Inst Inc, USA. 2018.
• Seyrek K, Karagenc T, Pasa S, Kıral F, Atasoy A. Serum zinc, iron and copper concentrations in dogs infected with Hepatozoon canis. Acta Vet Brno. 2009; 78: 471–475.
• Spears JW. Trace mineral bioavailability in ruminants. J Nut. 203; 133: 1506–1509.
• Stangl GI, Schwarz FJ, Kirchgessner M. Moderate long-term cobalt-deficiency affects liver, brain and erythrocyte lipids and lipoproteins of cattle. Nutr Res. 1999; 19(3): 415–427.
• Sykes AR, Coop RL, Robinson MG. Chronic subclinical ovine fascioliasis: Plasma glutamate dehydrogenase, gamma-glutamyl liver lipids in fatty liver of dairy cows. Anim Sci J. 1980; 77: 347–351.
• Şendil Ç, Bayşu N, Ünsüren H, Çelikkan M. Studies on the presence and the incidence of enzootic ataxie in our country. FÜ Vet Fak Derg. 1975; 2(1): 38–52.
• Taşçı S, Sengil AZ, Altindis M, Arisoy K. The effect of zinc supplementation in experimentally induced Toxoplasma gandii infection. J Egypt Soc Parasitol. 1995; 25: 745–751.
• Toparlak M, Tüzer E. Laboratory Techniques in the Diagnosis of Parasitic Diseases. Istanbul University Faculty of Veterinary Medicine Publication, Istanbul, Turkey. 1994; p. 19.
• Unal EF. Infertility in Small Ruminants, In:Birth and Infertility in Domestic Animals, Ed; Alaçam E, Medisan Yayınevi, Ankara, Turkey. 1987; pp. 195–212.
• Underwood EJ. Trace Elements in Human and Animal Nutrition. 4th Ed., Academic Press Inc., New York, USA. 1977; pp. 245-315.
• Vengust G, Klinkon M, Bidovec A, Vengust A. Fasciola hepatica: effects on blood constituents and liver minerals in fallow deer (Dama dama). Vet Parasitol. 2003; 112(1-2): 51–61.
• White CL, Martin GB, Hynd PI, Chopeman RE. The effect of zinc deficiency on wool growth and skin and wool follicle histology of male merino lambs. Br J Nutr. 1994; 71(3): 425–35.
• Yokus B, Cakir DU, Kurt D. Effects of seasonal and physiological variations on the serum major and trace element levels in sheep. Biol Trace Elem Res. 2004; 101(3): 241–255.