Bisphenol A'nın Rat Testis Gelişiminde Fonksiyon Gören Notch Sinyal Yolağı Üzerine Etkisi

Yıl 2021, Cilt 14, Sayı 4, 390 - 398, 31.12.2021

Özlem ÖZDEN AKKAYA Korhan ALTUNBAŞ Murat TOSUN Artay YAĞCI

https://doi.org/10.30607/kvj.957630

Öz

Endokrin bozucu kimyasallar arasında Bisphenol A (BPA) her yıl dünyada bir kaç milyon ton üretilmesi ve yaygın olarak kullanılmasıyla en çok dikkat çeken bir ajandır. BPA endokrin bozucu bir ajandır ve insanlar için karsinojen olarak sınıflandırılmaktadır. Fötal ve perinatal dönemde çevredeki dozlarla uyumlu olarak BPA’ya maruz kalmak nesiller boyunca erkek infertilitesinin bozulmasını indüklemektedir. Notch sinyal fonksiyonları hücrelerin farklılaşmasını, çoğalmasını ve canlı kalmasını düzenleyerek hücre kaderini belirler. Notch sinyal sistemi erkek germ hücrelerinin farklılaşması ve yaşamasında önemli bir rol oynamaktadır. Bu çalışmada in vivo embriyonal (E) 18-21, postnatal (P) 0-3 ve P4-7. günlerde BPA uygulamasını takiben rat testislerinde Notch I reseptör protein ekspresyonunun değişip değişmediğini araştırmak amaçlandı. Araştırmadan elde edilen sonuçlar, seçilen zaman dilimlerinde kullanılan BPA dozlarının sıçan testislerinde Notch 1 protein ekspresyonları üzerine etkisi olmadığını ortaya koydu.

Anahtar Kelimeler

Bisphenol A, Notch, sıçan, testis

The Effect of Bisphenol A on Notch Signaling Pathway in Development of Rat Testis

Öz

Bisphenol A (BPA) is the object of great concerns because of its widespread use, due to several millions of tons its production throughout the world each year. BPA is an endocrine disrupting chemical and is classified as a probable human carcinogen. Fetal and perinatal exposure to environmentally relevant doses of BPA induces multigenerational impairments of male fertility. Notch signaling functions to regulate cell-fate by modulating differentiation, proliferation, and survival of cells. Notch signaling system plays a significant role in male germ cell differentiation and survival. In this study, it was aimed to explore the alterations/changes in protein expression of Notch I receptor following BPA treatments during embriyonal (E) days 18 to 21, postnatal (P) days 0 to 3, and P4 to P7 time intervals in the rat testes in vivo. The results of study revealed that the used doses of BPA had’nt effect on Notch 1 protein expressions in rat testes during selected time preiods.

Anahtar Kelimeler

Bisphenol A, Notch, rat, testis

Kaynakça

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