Investigation of Anti-Müllerian Hormone Presence in Bitch Urine and Comparison of Anti-Müllerian Hormone Levels in Blood Serum and Urine

Yıl 2024, Cilt: 17 Sayı: 4, 418 - 422

Semra Kaya , Murat Can Demir , İnan Kaya , Muhammet Ali Karadağ , Gökhan Koçak , Cihan Kaçar

Öz

The presented study aimed to investigate the presence of AMH in bitch urine and to determine whether there is a correlation between blood serum and urine AMH levels. Forty-two healthy crossbreed bitches brought to Kafkas University, Faculty of Veterinary Medicine, Animal Hospital, Department of Obstetrics and Gynecology with a request for ovariohysterectomy were included in the study. Blood samples were taken from the Vena cephalica antebrachii, and urine samples were collected using a urinary catheter of all bitches. After the blood and urine samples were centrifuged, AMH levels were determined using the ELISA method. The average AMH concentration was determined to be 4.56±0.53 ng/mL in urine and 7.75±1.19 ng/mL in blood serum. No significant correlation was found between blood and urine AMH levels (P>0.05). It was concluded that urine AMH levels were not related to blood serum AMH levels.

Anahtar Kelimeler

Anti-Müllerian Hormone, Bitch, Blood serum, Urine

Köpek İdrarında Anti-Müllerian Hormon Varlığının Araştırılması ve Kan Serumu ve İdrar Anti-Müllerian Hormon Düzeylerinin Karşılaştırılması

Öz

Sunulan çalışmada köpek idrarında AMH varlığının araştırılması ve kan serumu ile idrar AMH düzeyi arasında korelasyon olup olmadığının belirlenmesi amaçlanmıştır. XXX Üniversitesi, Veteriner Fakültesi Hayvan Hastanesi, Doğum ve Jinekoloji Kliniği’ne ovaryohisterektomi isteğiyle getirilen sağlıklı 42 melez ırk köpek çalışmaya dahil edildi. Tüm köpeklerin Vena cephalica antebrahii’den kan örnekleri alındı ve idrar katateri kullanılarak idrar örnekleri toplandı. Kan ve idrar örnekleri santrifüj edildikten sonra AMH düzeyleri ELISA yöntemiyle belirlendi. Kan serumunda ortalama AMH düzeyi 7,75±1,19 ng.ml-1, idrarda ise 4,56±0,53 ng.ml-1 bulundu. Kan ve idrar AMH düzeyi arasında anlamlı bir korelasyon saptanmadı. Sonuç olarak bu çalışmada köpeklerde idrar AMH düzeyi ilk defa ölçülmüş ve kantitatif veri olarak sunulmuştur. İdrar AMH düzeyinin kan serumundaki AMH düzeyi ile ilişkili olmadığı ve idrar AMH düzeyine bakılarak köpeklerde ovaryum rezervi hakkında tahminde bulunmanın yanıltıcı olabileceği kanaatine varıldı.

Anahtar Kelimeler

Anti-Müllerian hormon, İdrar, Kan serumu, Köpek

Kaynakça

• Akbarinejad, V., Gharagozlou, F., Vojgani, M., & Ranji, A. (2020). Evidence for quadratic association between serum anti-Müllerian hormone (AMH) concentration and fertility in dairy cows. Animal Reproduction Science, 218, 106457. https://doi.org/10.1016/j.anireprosci.2020.106457
• Alm, H., & Holst, B.S. (2018). Identifying ovarian tissue in the bitch using anti-Müllerian hormone (AMH) or luteinizing hormone (LH). Theriogenology, 106, 15-20. https://doi.org/10.1016/j.theriogenology.2017.09.028.
• Anadol, E., Gultiken, N., Yarim, G.F., Karaca, E., Kanca, H., & Yarim, M. (2020). Investigation of diagnostic use of serum anti-Müllerian hormone concentration in dioestrus and anoestrus bitches before and after ovariohysterectomy and the relationship with ovarian follicle numbers. Polish Journal of Veterinary Sciences, 23(3), 391-397. https://10.24425/pjvs.2020.134683
• Anadol, E., Yar Sağlam, A.S., Yarim, G.F., & Gultiken N. (2016). Serum concentrations of anti-Müllerian hormone and its expression in the remnant ovarian tissue of rats with experimentally induced ovarian remnant syndrome. Kafkas Universitesi Veteriner Fakültesi Dergisi, 22(4), 545-550. https://10.9775/kvfd.2016.15006 Axnér, E., & Ström Holst, B. (2015). Concentrations of anti-Müllerian hormone in the domestic cat. Relation with spay or neuter status and serum estradiol. Theriogenology, 83, 817-21. https://doi.org/10.1016/J.THERIOGENOLOGY.2014.11.016.
• Bedenk, J., Vrtačnik-Bokal, E., & Virant-Klun, I. (2020). The role of anti-Müllerian hormone (AMH) in ovarian disease and infertility. Journal of Assisted Reproduction and Genetics, 37, 89-100. https://doi.org/10.1007/S10815-019-01622-7.
• Cai, Q., Jin, S., Zong, H., Pei, L., Cao, K., Lingbo, Q., & Li, Z. (2023). A quadruplex ultrasensitive immunoassay for simultaneous assessment of human reproductive hormone proteins in multiple biofluid samples. Analytical Chemistry, 95(31), 11641-11648. https://doi.org/10.1021/acs.analchem.3c01399
• Griesinger, G., Dafopoulos, K., Buendgen, N., Cascorbi, I., Georgoulias, P., Zavos, A., Messini C.I., & Messinis, I.E. (2012). Elimination half-life of anti-Müllerian hormone. Journal of Clinical Endocrinology and Metabolism, 97, 2160-2163. https://doi.org/10.1210/JC.2012-1070.
• Hallberg, I., Olsson, H., Lau, A., Wallander, S., Snell, A., Bergman, D., & Ström Holst, B.S. (2024). Endocrine and dog factors associated with semen quality. Scientific Reports, 14: 718. https://doi.org/10.1038/s41598-024-51242-0
• Hollinshead, F.K., Walker, C., & Hanlon, D.W. (2017). Determination of the normal reference interval for anti-Müllerian hormone (AMH) in bitches and use of AMH as a potential predictor of litter size. Reproduction Domestic Animals, 52 (Suppl.2): 35-40. 1 https://0.1111/rda.12822.
• Holst, B.S. (2017). Diagnostic possibilities from a serum sample-Clinical value of new methods within small animal reproduction, with focus on anti-Müllerian hormone. Reproductin Domestic Animals, 52 (Suppl.2), 303-309. https://10.1111/rda.12856.
• Ipandi I. (2024). Analysis of anti Mullerian Hormone (AMH) levels in urine and blood in polycystic ovary syndrome (PCOS) Patients, 08.08.2024, https://repository.unair.ac.id/92797/1/abstrak.pdf
• Kaya, S., Taşcı, S.K., Demir, M.C., Karadağ, M.A., Kumcu, M.S., Kaçar, C., Ermutlu, D.G., & Kuru, M. (2024). Determination of anti-Müllerian hormone levels in blood and urine in fertile cats. Theriogenology, 224, 102-106. https://10.1016/j.theriogenology.2024.05.016
• Kaya, S., Akın, G., Koçak, G., & Kaçar, C. (2021). Clinical Use of Anti-Mullerian Hormone in Female Cats and Dogs. Bozok Veterinary Sciences, 2(2), 57-61.
• Kostrzewa, M., Głowacka, E., Stetkiewicz, T., Mariusz Grzesiak, M., Szyłło, K., Stachowiak, G., & Wilczyński, J.R. (2020). Is serum anti-Müllerian hormone (AMH) assay a satisfactory measure for ovarian reserve estimation? A comparison of serum and peritoneal fluid AMH levels. Advances in Clinical and Experimental Medicine, 29(7), 853-856. https://10.17219/acem/121010
• Muhammed, T.M., Saleem, H.M., & Almawla, S.O. (2018). Evaluation of seminal plasma anti-Mullerian hormone levels and their association with sperms’ count and activity in infertile males. Indian Journal of Public Health Research & Development, 9(11), 1916-1920. https://10.5958/0976-5506.2018.01729.1
• Pankhurst, M.W., Leathart, B.A., Batchelor, N.J., & McLennan, I.S. (2016). The Anti-Müllerian Hormone Precursor (proAMH) Is Not Converted to the Receptor-Competent Form (AMHN,C) in the Circulating Blood of Mice. Journal of the Endocrine Society, 157, 1622-1629.
• Pankhurst, M.W., & McLennan, I.S. (2016). A specific immunoassay for proAMH, the uncleaved proprotein precursor of anti-Müllerian hormone. Molecular and Cellular Endocrinology, 419, 165e17. http://dx.doi.org/10.1016/j.mce.2015.10.013
• Sriraman, V., Niu, E., Matias Jr R., Donahoe, P.K., Maclaughlin, DtT., Hardy, M.P., & Lee, M.M. (2001). Müllerian inhibiting substance inhibits testosterone synthesis in adult rats. Journal of Andrology, 22, 750-758.
• Themmen, A.P.N., Kalra, B., Visser, J.A., Kumar, A., Savjani, G., de Gier, J., & Jaques, S. (2016). The use of anti-Müllerian hormone as diagnostic for gonadectomy status in dogs. Theriogenology, 86, 1467-1474. http://dx.doi.org/10.1016/j.theriogenology.2016.05.004.
• Yagci, I.P., Pekcan, M., Polat, I.M., Kalender, H., & Macun, H.C. (2016). Does serum anti-Müllerian hormone levels always discriminate presence of the ovaries in adult bitches? Comparison of two ELISA kits. Reproduction Domestic Animals, 51, 910-915. http://10.1111/rda.12757.
• Yağcı, I.P., Polat, İ.M, Pekcan, M. (2016). Evaluation of age releated Anti-Müllerian hormone variations in domestic cat. Kafkas Univ Vet Fak Derg, 22(5), 729-732. http://dx.doi.org/10.9775/kvfd.2016.15288
• Yilmaz, Ö.T., Toydemir, T.S.F., Kirsan, İ., Ucmak, Z.G., & Karacam, E.C. (2015). Anti-Müllerian hormone as a diagnostict tool for ovarian remnant sydrome in bitches. Veterinary Research Communications, 39, http://159-162. 10.1007/s11259-015-9639-0.