Presence of Escherichia Coli in Urfa Cheese and In Vitro Screening of Donkey Milk and Essential Oil of Micromeria Congesta for Antibacterial Activity Using Disc Diffusion Method

Öz

The aim of this study was to determine the presence and antibiotic resistance profile of Escherichia coli strains in Urfa cheese and comparing the antibacterial activity of donkey milk and essential oil of Micromeria congesta. 93 fresh Urfa cheese samples were obtained in Şanlıurfa province. Bacteria were isolated and identified by conventional cultural technique. In vitro susceptibility test for antibiotics, donkey milk and essential oil of M .congesta  were performed with Kirby-Bauer disc diffusion method. Steam distillation was used to provide the essential oil of Micromeria congesta. E.coli strains were isolated in 43 (46%) of all cheese samples. The E.coli were highly resistant to clindamycin (100%), penicillin (100%), oxacillin (100%), tetracycline (100%), neomycin (80%), kanamycin (80%), gentamycin  (50%), enrofloxacin (50%), streptomycin (46%) and low level of resistance to  imipenem (20%), cefoxitin (16%). This study showed E.coli strains developed high rates of multidrug resistance. Essential oil of Micromeri congesta and donkey milk inhibited a high antibacterial activity on E.coli strains when comparing the antibacterial activity of reference antibiotics (tetracycline and streptomycin).

Anahtar Kelimeler

• Urfa cheese,donkey milk,Micromeria congesta

Urfa Peynirlerinde Escherichia Coli’nin Varlığı, Eşek Sütü ve Micromeria Congesta Esansiyel Yağının Antibakteriyel Aktivitesinin Disk Difüzyon Yöntemiyle İn Vitro Görüntülenmesi

Öz

Bu çalışmanın amacı, Urfa peynirlerinde Escherichia coli suşlarının varlığını ve antibiyotik direnç profilini belirlemek, eşek sütü ve Micromeria congesta bitkisi esansiyel yağının antibakteriyel etkinliğini karşılaştırmaktır. Çalışma kapsamında, Şanlıurfa ilinden 93 adet taze Urfa peyniri örneği toplandı. Konvansiyonel kültür tekniği ile izolasyon ve identifikasyon gerçekleştirildi. Kirby-Bauer disk difüzyon yöntemi ile antibiyotik, eşek sütü ve Micromeria congesta uçucu yağı için in vitro duyarlılık testi yapıldı. Micromeria congesta’nın uçucu yağını elde etmek için buhar distilasyon yöntemi kullanıldı. Tüm peynir örneklerinin 43’ünde (% 46) E. coli suşu izole edildi. E. coli suşları, klindamisin (% 100), penisilin (% 100), oksasilin (% 100), tetrasiklin (% 100), neomisin (% 80), kanamisin (% 80), gentamisin (% 50) enrofloksasin (% 50), streptomisin (% 46)’e yüksek direnç; imipenem (% 20), sefoksitin (% 16)’ine de düşük direnç gösterdi. Bu çalışma E. coli suşlarının yüksek oranda çoklu ilaç direnci geliştirdiğini gösterdi. Micromeria congesta esansiyel yağı ve eşek sütünün antibakteriyel etkinliği referans antibiyotikler (tetrasiklin ve streptomisin) ile kıyaslandığında antibakteriyel etkinliğinin daha etkin olduğu görüldü.

Anahtar Kelimeler

• Urfa cheese,donkey milk,Micromeria congesta,antibacterial activity

Kaynakça

1. 1. Yangılar F, Kızılkaya PÇ. Determination of physical, chemical and microbiological properties of aromatic Ardahan cecil cheese. BEU Journal of Science. 2015; 4(2), 122-130.
2. 2. Atasoy FA, Türkoğlu H, Özer BH. Some microbiological properties of raw milk, yogurt and fresh Urfa cheese produced and sold in Şanlıurfa province. J.Agric Fac. HR.U. 2003; 7 (3-4): 77-83.
3. 3. Coia JE. Clinical, microbiological and epidemiological aspects of Escherichia coli O157 infection.FEMS Immunology and Medical Microbiology.1998; 20: 1-9.
4. 4. Ribeiro T, Oliveira M, Fraqueza MJ, Lauková A, Elias M, Tenreiro, R, Barreto, AS, Semedo-Lemsaddek T. Antibiotic resistance and virulence factors among enterococci isolated from Chouriço, a traditional Portuguese dry fermented sausage. J. Food Protect. 2011; 74: 465-469.
5. 5. Vrabec M, Lovayová V, Dudriková K, Gallo J, Dudriková E. Antibiotic resistance and prevalence of Enterococcus spp. and Escherichia coli isolated from bryndza cheese. Ital. J Anim Sci. 2015; 14: 3968.
6. 6. Aslan S, Özdemir F. Extended spectrum beta-lactamases in Escherichia coli strains isolated from homemade white cheeses prevalence and antibiotic susceptibility. World J Microbiol Biotechnol. 2008; 24: 2361–2364.
7. 7. Bengtsson B, Unnerstad HE, Ekman T, Artursson K, Nilsson-Öst M, Waller KP. Antimicrobial susceptibility of udder pathogens from cases of acute clinical mastitis in dairy cows. Vet Microbiol. 2009; 136: 142-149.
8. 8. Dinç G, Ata Z, Temelli S. Sığır mastitislerinden izole edilen Escherichia coli suşlarında genişlemiş spektrumlu beta-laktamaz aktivitesi ve antibiyotik dirençlilik profilinin incelenmesi. Ankara Üniv Vet Fak Derg. 2012; 59: 85-88.

9. 9. Srinivasan V, Gillespie BE, Lewis MJ, Nguyen LT, Headrick SI, Schukken YH, Oliver SP. Phenotypic and genotypic antimicrobial resistance patterns of Escherichia coli isolated from dairy cows with mastitis. Vet Microbiol. 2007; 124: 319-328.
10. 10. Sumathi BR, Amitra RG, Krishnappa G. Antibiogram profile based dendogram analysis of Escherichia coli serotypes isolated from bovine mastitis. Vet World. 2008; 1: 37-39.
11. 11. Davies J, Davies D. Origins and evolution of antibiotic resistance. Microbiol Mol Biol Rev. 2010; 74(3): 417-33.
12. 12. Shah AA, Hasan F, Ahmet S, Hameed A. Extended -spectrum beta-lactamases (ESbLs) characterization, epidemiology and detection. Crit Rev Microbiol. 2004; 30(1): 25-32.
13. 13. Kaya S, Baydan E. Meme içi ilaç hareketi ve meme hastalıklarının sağaltımı, Türk Vet. Hek. Derg. 1999; 11: 34-47.
14. 14. Yıldız A. Laktasyondaki subklinik ve klinik mastitisli sütçü ineklerde lincomysin-neomycin kombinasyonuyla meme içi tedavinin etkinliği. F.Ü Sağlık Bil. Dergisi. 2003; 17(1): 65-69.
15. 15. Kahlmeter G, Brown DF, Goldstein FW, MacGowan AP, Mouton JW, Odenholt I, Rodloff A, Soussy CJ, Steinbakk M, Soriano F, Stetsiouk O. European Committee on antimicrobial susceptibility testing (EUCAST) technical notes on antimicrobial susceptibility testing. Clin Microbiol Infect. 2006; 12: 501-503.
16. 16. Herken EN, Çelik A, Aslan M, Aydınlık N. The constituents of Essential Oil: Antimicrobial and Antioxidant Activity of Micromeria congesta Boiss&Hauskn.ex Boiss.from East Anatolia. J.Med Food. 2012; 15(9): 835-839.
17. 17. Akan H, Korkut MM, Balos MM. Arat Dağı ve Çevresinde (Birecik, Şanlıurfa) Etnobotanik bir Araştırma. Sci Eng J Fırat Univ. 2008; 20: 67–81.
18. 18. Baytop, T. Therapy with medicinal plants in Turkey (Past and Present). Nobel Tıp publishing; 1999.
19. 19. Şahintürk M, Öner Z. Eşek sütünün Özelliklerinin Belirlenmesi. Türkiye 12. Gıda Kongresi. Trakya Üniversitesi, Edirne. 2016; 154.
20. 20. Bauer AW, Kirby WM, Sherris JC, Turck M. Antibiotic susceptibility testing by a standardized single disk method. Amer. J. Clin. Pathol. 1966; 45: 493-496.
21. 21. Clinical and Laboratory Standards Institute (CLSI) (2012). Performance standards for antimicrobial susceptibility testing. 32:3, CLSI, Wayne, PA.
22. 22. Kaper JB, Nataro JP, Mobley HLT. Pathogenic Escherichia coli. Nat. Rev.Microbiol. 2004; 2: 123–140.
23. 23. Vernozy-Rozand C, Mazuy-Cruchaudet C, Bavai C, Montet MP, Bonin V, Dernburg A, Richard Y. Growth and survival of Escherichia coli O157:H7 during the manufacture and ripening of raw goat milk lactic cheeses.Int. J. Food Microbiol. 2005; 105: 83–88.
24. 24. Solomakos N, Govaris A, Angelidis AS, Pournaras S, Burriel AR, Kritas SK, Papageorgiou DK. Occurrence, virulence genes and antibiotic resistance of Escherichia coli O157 isolated from raw bovine, caprine and ovine milk in Greece. Food Microbiology. 2009; 26(8): 865-871.
25. 25. Govaris A, Koidis P, Papatheodorou K. Behaviour of Escherichia coli O157:H7 in sour milk, cow’s milk yogurt and ewe’s milk yogurt. J. Dairy Res. 2002; 69: 655–660.
26. 26. Govaris A, Papageorgiou D, Papatheodorou K. Behaviour of Escherichia coli O157:H7 during the manufacture and ripening of Feta and Telemes cheese.J. Food Prot.2002; 65: 609–615.
27. 27. Caro I, Mateo J, Garcı´a-Armesto MR. Phenotypical characteristics of Shigalike toxin Escherichia coli isolated from sheep dairy products. Lett. Appl.Microbiol. 2007; 45: 295–300.
28. 28. Lira WM, Macedo C, Marin JM. The incidence of Shiga toxin-producing Escherichia coli in cattle with mastitis in Brazil. J. Appl. Microbiol. 2004; 97: 861–866.
29. 29. Momtaz H, Farzan R, Rahimi E, Safarpoor Dehkordi F, Souod N. Molecular characterization of Shiga toxin-producing Escherichia coli isolated from ruminant and donkey raw milk samples and traditional dairy products in Iran. Scientific World J 2012; 1–13.
30. 30. Stephan R, Schumacher S, Corti S, Krause G, Danuser J, Beutin L. Prevalence and characteristics of Shiga toxin-producing Escherichia coli in Swiss raw milk cheeses collected at producer level. J Dairy Sci. 2008; 91: 2561–2565.
31. 31. Aslani MM, Alikhani MY, Zavari A, Yousefi R, Zamani AR. Characterization of enteroaggregative Escherichia coli (EAEC) clinical isolates and their antibiotic resistance pattern. Int J Infect Dis. 2011; 15: 136–139.
32. 32. Tabaran A, Mihaiu M, Tăbăran F, Colobatiu L, Reget O, Borzan MM, Dan SD. First study on characterization of virulence and antibiotic resistance genes in verotoxigenic and enterotoxigenic E. coli isolated from raw milk and unpasteurized traditional cheeses in Romania. Folia Microbiologica. 2017; 62(2): 145-150.
33. 33. Paneto BR, Schocken-Iturrino RP, Macedo C, Santo E, Marin JM. Occurrence of toxigenic Escherichia coli in raw milk cheese in Brazil. Arq Bras Med Vet Zootec. 2007; 59: 508–512.