Determination of antioxidant and antimicrobial activities of ethanolic extract from Suillus granulatus

Öz

In this study, Suillus spp. after determining the dry matter and protein amounts of the mushroom, the antioxidant and antimicrobial activities of the ethanolic extracts were investigated. Free radical scavenging activity was determined by DPPH method and phenolic content was determined by HPLC. Antimicrobial activity of Suillus spp. was determined against microorganisms that Staphylococcus aureus, Escherichia coli, Salmonella Typhimurium, Listeria monocytogenes and Pseudomonas florescens microorganisms. Suillus mushroom had a antioxidant activity and It was determined that the phenolic components with the highest amount of phenolic acid were ellagic acid, caffeic acid and gentisic acid, respectively. It was stated that Suillus extract inhibited the growth of S. aureus and E. coli, while suppressing the growth of S. Typhimurium, L. monocytogenes and P. florescens.

Anahtar Kelimeler

• Suillus
• antioxidant
• antimicrobial
• Burdur

Suillus granulatus’tan elde edilen etanolik ekstraktın antioksidan ve antimikrobiyal aktivitelerinin belirlenmesi

Öz

Çalışmamızda, Suillus spp. mantarının kurumadde ve protein miktarları belirlendikten sonra etanolik ekstraksiyonlarının antioksidan ve antimikrobiyal aktiviteleri araştırılmıştır. Serbest radikal süpürme aktivitesi DPPH yöntemiyle belirlenirken fenolik madde içeriği HPLC cihazıyla tespit edilmiştir. Suillus spp.’nin antimikrobiyal aktivitesi Staphylococcus aureus, Escherichia coli, Salmonella Typhimurium, Listeria monocytogenes ve Pseudomonas florescens mikroorganizmalarına karşı belirlenmiştir. Suillus spp. mantarının antioksidan kapasiteye sahip olduğu ve fenolik asit bakımından en yüksek miktarda bulunan fenolik bileşenlerin sırasıyla ellajik asit, kafeik asit ve gentisik asit olduğu tespit edilmiştir. Suillus ekstraktının S. aureus ve E. coli gelişimini inhibe ederken S. Typhimurium, L. monocytogenes ve P. florescens’in gelişimini baskıladığı tespit edilmiştir.

Anahtar Kelimeler

• Suillus
• antioksidan
• antimikrobiyal
• Burdur
• Suillus, antioksidan, antimikrobiyal, Burdur

Kaynakça

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