The Protective Effects of Egg Yolks of Different Poultry Species in Domestic Animal Semens Cryopreservation

Yıl 2017, Cilt: 2 Sayı: 1, 51 - 59, 13.09.2017

Burcu Yalçın , Eser Akal

https://doi.org/10.24880/maeuvfd.289172

Öz

Until
today, experimentally designed technical adjustments have been introduced for
many types in semen freezing, which has become a requirement for many fields such
as artificial insemination, species protection and clinical medicine. Egg yolk,
provided that it is added as an additive in many diluents used for freezing
mammalian sperm; has taken place thanks to the protective effects of the
contained special agents. For this purpose, it widely used and has an effect on
motility, viability and fertilization capacity. Protein, lipid and cholesterol
present in the egg yolk are available to actively protect the sperm during
various stages of freezing. In order to achieve better semen quality, the use
of egg yolks of different poultry species instead of chicken egg yolks, which
are traditionally used, has been tried and positive results have been obtained,
albeit different according to the animal species. This condition may be due to
the differences in biochemical content of egg yolks. These chemical differences
can be explained by post-thaw motility and integrity differences in semen
freezing process with extenders, which produced from egg yolks of different
poultry species. The positive results were obtained in freezing of stallion,
buffalo, bull, ram and boar semen, using of duck, goose, quail, pigeon, turkey and
partridge egg yolks instead of chicken egg yolk. It has been concluded that egg
yolks of different poultry species can be used as an alternative
cryoprotectants instead of chicken egg yolk. However, this assessment needs to
be retried for dose planning to become more clear.

Anahtar Kelimeler

Different poultry types, egg yolk, semen extender, semen freezing, domestic animal, sperm, protective effects

Evcil Hayvan Spermalarının Dondurulmasında Farklı Kanatlı Türü Yumurta Sarılarının Koruyucu Etkileri

Öz

Günümüze
kadar suni tohumlama, tür koruma ve klinik tıp gibi birçok alanda gereksinim
haline gelen sperma dondurulmasında, birçok tür için deneysel olarak
tasarlanmış teknik ayarlamalar kullanıma girmiştir. Memeli spermalarının
dondurulması için kullanılan birçok sulandırıcı içerisinde katkı maddesi olarak
ilave edilmesi koşuluyla yumurta sarısı; içerdiği özel ajanların koruyucu
etkileri sayesinde yerini almıştır. Bu amaçla geniş çapta kullanılmakta ve
spermatozoon motilitesi, canlılığı ve fertilizasyon kapasitesi üzerinde etkiye
sahiptir. Yumurta sarısında bulunan protein, lipit ve kolesterol dondurma
sürecinde çeşitli aşamalar boyunca spermayı aktif olarak korumak için
mevcutturlar. Daha iyi çözüm sonu sperma kalitesi elde etmek amacıyla farklı
kanatlı türlerinin yumurta sarılarının geleneksel olarak kullanılan tavuk
yumurta sarısı yerine kullanımı denenmiş ve hayvan türlerine göre farklı
olmakla birlikte olumlu sonuçlar alınmıştır. Bu durum yumurta sarılarının
biyokimyasal içeriklerinin farklılığından kaynaklanabilir. Bu kimyasal
farklılıklar; farklı kanatlı türlerinin yumurta sarılarının oluşturduğu
sulandırıcılarla yapılan sperma dondurulması işlemindeki çözüm sonu motilitesi
ve spermatozoon bütünlüğü farklılıklarıyla açıklanabilir. Aygır, manda, boğa, koç
ve domuz spermalarının dondurulmasında tavuk yumurta sarısı yerine kullanılan
ördek, kaz, bıldırcın, güvercin, hindi ve keklik yumurta sarıları ile olumlu
sonuçlar elde edilmiştir. Farklı kanatlı türlerinin yumurta sarılarının, tavuk
yumurta sarısı yerine alternatif bir kriyoprotektan olarak kullanılabileceği
fakat bu değerlendirmenin daha net olabilmesi için doz planlamalarının ayrı
ayrı denenmesi gerektiği sonucuna varılmıştır.

Anahtar Kelimeler

sperma dondurma, sulandırıcı, yumurta sarısı, Evcil hayvan, farklı kanatlı türleri, koruyucu etkiler, sperma

Kaynakça

• 1. Holt WV, 2000: Basic aspects of frozen storage of semen. Anim Reprod Sci, 62, 3–22.
• 2. Phillips PH, Lardy HA, 1940: A yolk-buffer pablum for the preservation of bull semen. J Dairy Sci, 23, 399-404.
• 3. Watson P, Martin I, 1975: Effects of egg yolk, glycerol and the freezing rate on the viability and
• acrosomal structures of frozen ram spermatozoa. Aust J Biol Sci, 28, 153-159.
• 4. Johnson LA, Weitze KF, Fiser P, Maxwell WM, 2000: Storage of boar semen. Anim Reprod Sci, 62, 143-172.
• 5. Salamon S, Maxwell WM, 2000: Storage of ram semen. Anim Reprod Sci, 62, 77–111.
• 6. Barbas JP, Mascarenhas RD, 2009: Cryopreservation of domestic animal sperm cells. Cell Tissue Bank, 10, 49–62.
• 7. Milovanov VK, Sokolovskaja II, 1980: The results and perspectives of scientific research. Zhivotnovodstvo, 10, 48–50.
• 8. Purdy PH, 2006: A review on goat sperm cryopreservation. Small Rum Res, 6, 215–225.
• 9. Medeiros CM, Forell F, Oliveira AT, Rodrigues JL, 2002: Current status of sperm cryopreservation: why isn’t better. Theriogenology, 57, 327–344.
• 10. Bathgate R, Maxwell WMC, Evans G, 2006: Studies on the effect of supplementing boar semen cryopreservation media with different avian egg yolk types on in vitro post-thaw sperm quality. Reprod Dom Anim, 41, 68–73.
• 11. Cross NL, 1998: Role of cholesterol in sperm capacitation. Biol Reprod, 59, 7–11.
• 12. Bailey JL, Bilodeau JF, Cormier N, 2000: Semen cryopreservation in domestic animals: A damaging and capacitating phenomenon. J Androl, 21, 1-7.
• 13. Agarwal A, Prabakaran SA, Said TM, 2005: Prevention of oxidative stress injury to sperm. J Androl, 26, 654-660.
• 14. Gholami M, Faraji Z, Zamiri MJ, 2012: Effect of egg yolk of four avian species on the cryopreserved ram spermatozoa. Iran J Agric Res, 13(1), 23-27.
• 15. Phillips PH, 1939: Preservation of bull semen. J Biol Chem, 130, 415.
• 16. Pace MM, Graham EF, 1974: Components in egg yolk which protect bovine spermatozoa during freezing. J Anim Sci, 39, 1144–1149.
• 17. Blackshaw AW, 1954: The prevention of temperature shock of bull and ram semen. J Biol Sci, 7, 573-582.
• 18. Madden TD, Chapman D, Quinn PJ, 1979: Cholesterol modulates activity of calcium-dependent ATPase of the sarcoplasmic reticulum. Nature Lond, 279, 538-541.
• 19. Quinn PJ, White IG, Cleland KW, 1969: Chemical and ultrastructural changes in ram spermatozoa after washing, cold shock and freezing. J Reprod Fert, 18, 209-220.
• 20. Trimeche A, Anton M, Renard P, Gandemer G, Tainturier D, 1997: Quail egg yolk: a novel cryoprotectant for the freeze preservation of Poitou jackass sperm. Cryobiol, 34, 385–393.
• 21. Foulkes JA, Sweasey D, Goodey RG, 1980: Fertility of bull spermatozoa in egg-yolk diluents of varied lipid fatty acid composition. J Reprod Fertil, 60, 165–169.
• 22. Bergeron A, Manjunath P, 2006: New insightas towards understanding the mechanisms of sperm protection by egg yolk and milk. Mol Reprod Dev, 73, 1338–1344.
• 23. Humes R, Webb G, 2006: Use of chicken or chukar egg yolk with two cryoprotectants for preservation of stallion semen. Anim Reprod Sci, 94, 62–63.
• 24. Clulow JR, Maxwell WMC, Evans G, Morris LHA, 2007: A comparison of duck and chicken egg yolk for cryopreservation of stallion sperm. Aust Vet J, 85(6), 232-235.
• 25. Akhter S, Rakha BA, Andrabi SMH, Ansari MS, 2010: Comparison of egg yolks from three avian species in extender for cryopreservation of Sahiwal bull epididymal spermatozoa. Anim Sci Pap Rep Anim, 29(2), 131-138.
• 26. El-Sheshtawy RI, El-Sisy, G, Mohamed A, El-Natat WS, 2010: Effect of egg yolk from different avian species on cryopreservability of buffalo semen. Global J Biotechnol Biochem, 5, 211-215.
• 27. Kazmierska M, Jarosz B, Korzeniowska M, Trziszka T, Dobrzanski Z, 2005: Comparative analysis of fatty acid profile and cholesterol content of egg yolks of different bird species. Pol J Food Nutr Sci, 14(55), 69-73.
• 28. Bair CW, Marion WW, 1978: Yolk cholesterol in eggs from various avian species. Poult Sci, 57, 1260–1265.
• 29. Choi Y, Park Y, Pariza MW, Ntambi JM, 2001: Regulation of stearoyl-CoA desaturase activity by the trans-10, cis-12 isomer of conjugated linoleic acid in HepG2 cells. Biochem Bioph Res Co, 284, 689-693.
• 30. Golzar Adabi SH, Ahbab M, Fani AR, Hajbabaei A, Ceylan N, Cooper RG, 2013: Egg yolk fatty acid profile of avian species – influence on human nutrition. J Anim Physiol Anim Nutr, 97, 27-38.
• 31. Kulaksiz R, Cebi C, Akcay E, Daskin A, 2010: The protective effect of egg yolk from different avian species during the cryopreservation of Karayaka ram semen. Small Rumin Res, 88, 12-15.
• 32. Purdy PH, Graham JK, 2004: Effect of adding cholesterol to bull sperm membranes on sperm capacitation, the acrosome reaction and fertility. Biol Reprod, 71, 522-527.
• 33. Juyena N, Stelletta C, 2012: Review seminal plasma: an essential attribute to spermatozoa. J Androl, 33(4), 536-551.
• 34. Hafez ESE, 1987: Semen evaluation reproduction in farm animals. 5nd ed., Lea and Febiger Press, Philadephia,USA.
• 35. Jain YC, Anand SR, 1976: The lipids of buffalo spermatozoa and seminal plasma. J Reprod Fertil, 47, 255–260.
• 36. Andrabi SMH, 2009: Factors affecting the quality of cryopreserved buffalo (Bubalus bubalis) bull spermatozoa. Reprod Dom Anim, 44, 552–569.
• 37. Su L, Li X, Quan J, Yang S, Li Y, He X, Tang X, 2008: A comparison of the protective action of added egg yolks from five avian species to the cryopreservation of bull sperm. Anim Reprod Sci, 104, 212-219.