Isolation of Dermatophytes from Cats and Dogs

Yıl 2017, Cilt: 2 Sayı: 2, 125 - 130, 29.12.2017

Özlem Şahan Yapıcıer , Ezgi Şababoğlu , Dilek Öztürk , Faruk Pehlivanoğlu , Mehmet Kaya Hülya Türütoğlu

https://doi.org/10.24880/maeuvfd.359535

Cited By: 1

Öz

Dermatophytosis
is an infectious and zoonotic disease caused by fungal agents that affect the
keratin layer of hair follicles, nails and epidermis. The causative agents of
dermatophytosis are Microsporum, Trichophyton and Epidermophyton species. In this study, a
total of 102 skin scarpings and hair samples taken from cats and dogs with
dermatophytosis suspicion between 2013 and 2017 and at different seasons were investigated
at Mehmet Akif Ersoy University Faculty of Veterinary Medicine Microbiology
Laboratory. The mycological analyses were conducted by direct microscopy and
fungal culture on Sabouraud Dextose Agar. Fifty six out of 102 samples were
positive for dermatophytosis. Five (24.83%) Microsporum sp. and 3 (12.50 %) Trichophyton sp. were isolated from 24 materials taken from the cats. Fourty seven
(60.25%) Microsporum sp.  and 1 (1.28%) Trichophyton sp. were
diagnosed from 78 dog based materials. Dermatophytosis
cases were mostly seen in spring with the rate of 82.75% and were also found in
autumn (%64.28) and summer (% 38.88) seasons in cats and dogs.    

Anahtar Kelimeler

Dermatophytosis, isolation, cat

Kedi ve Köpeklerden Dermatofitlerin İzolasyonu

Öz

Dermatofitozis, fungal etkenler tarafından oluşturulan, kıl
folikülleri, tırnak ve epidermisin keratin tabakasını etkileyen infeksiyöz ve
zoonoz bir hastalıktır. Dermatofitozise neden olan etkenler Microsporum,
Trichophyton ve Epidermophyton cinsinde bulunmaktadır. Bu çalışmada,
2013-2017 yılları arasında ve farklı mevsimlerde kedi ve köpeklerden alınarak Mehmet
Akif Ersoy Üniversitesi Veteriner Fakültesi Mikrobiyoloji Laboratuvarı’na dermatofitozis
şüpheli olarak gönderilen toplam 102 deri kazıntısı ve kıl örneği incelendi. Örneklerin
mikolojik analizi direkt mikroskopi ve Sabouraud Dekstroz Agara ekim yöntemleri
ile yapıldı. Toplam 102 örneğin 56 (%54.90)’sı dermatofitozis yönünden pozitif
bulundu. Kedilerden alınan 24 materyalin 5 (%20.83)’inden Microsporum sp., 3 (%12.5)’ünden ise Trichophyton sp.
izole edildi. Köpek orijinli 78 materyalde 47 (%60.25) Microsporum sp. ve 1 (%1.28) Trichophyton sp. teşhis edildi. Kedi ve köpeklerde dermatofitozis
olgularına çoğunlukla ilkbaharda % 82.75 oranında olmak üzere sonbahar (% 64.28)
ve yaz (% 38.88) mevsimlerinde de rastlandı.

Anahtar Kelimeler

Dermatofitozis, izolasyon, kedi

Kaynakça

• 1. Arda M. Temel Mikrobiyoloji. In: Arda M, ed. Mantarların genel karakterleri. Genişletilmiş 2. Baskı. p.315-367. Ankara: Medisan Yayınevi; 2000.
• 2. Babacan O, Bas B, Müstak HK, Sahan O, Tekin O, Torun E. Retrospective evolution of dermatophytes isolated from cats and dogs. Etlik Vet Microbiol Derg. 2011; 22:23–26.
• 3. Brilhante RSN, Cavalcante CSP, Soares-Junior FA, Cordeiro RA, Sidrim JJC, Rocha MFG. High rate of Microsporum canis feline and canine dermatophytoses in Northeast Brazil: epidemiological and diagnostic features. Mycopathologia. 2003; 156: 303-308. 

• 4. Cabanes FJ, Abarca ML, Bragulat MR. Dermatophytes isolated from domestic animals in Barcelona, Spain. Mycopathologia. 1997; 137: 107–113.
• 5. Cafarchia C, Romito D, Sasanelli M, Lia R, Capelli G, Otranto D. The epidemiology of canine and feline dermatophyoses in southern Italy. Mycoses. 2004; 47: 508-513.
• 6. Copetti MV, Santurio JM, Cavalheiro AS, Boeck AA, Argenta JS, Aguiar LC, Alves SH. Dermatophytes isolated from dogs and cats suspected of dermatophytosis in Southern Brazil. Acta Scientiae Veterinariae. 2006; 34: 119-124.
• 7. Çiftçi A, İça T, Sareyyüpoğlu B, Müştak HK. Kedi ve köpek dermatofitozlarından izole edilen mantarların retrospektif değerlendirilmesi. Ankara Üniv Vet Fak Derg. 2005; 52:45-48.
• 8. Deboer DJ, Moreillo KA. The immune response to Microsporum canis indused by a fungal cell wall vaccine. Vet Derm. 1994; 5: 47-55. 
 9. Dicle Ö, Özkesici B: Tinea capitis. Turk J Dermatol. 2013; 7: 1-8.
• 10. Elewski B. Tinea capitis: a current perspective. J Am Acad Dermatol. 2000; 42:1- 20.
• 11. Foil CS. Dermatophytosis. In: Greene, CE, ed. Infectious Diseases of the Dog and Cat. p: 362–370. Philadelphia: W.B. Saunders; 1998. 12. Fuller LC, Child FJ, Midgley G, Higgins EM. Diagnosis and management of scalp ringworm. BMJ. 2003; 326:539-541. 

• 13. Guzman-Chavez RE, Segundo-Zaragoza C, Cervantes-Olivares RA. Presence of keratinophilic fungi with special reference to dermatophytes on the haircoat of dogs and cats in Mexico and Nezahualcoyotl cities. Rev Latinoam Microbiol. 2000; 42:41-44.
• 14. Hainer BL. Dermatophyte infections. Am Fam Physician. 2003; 67:101-108.
• 15. Higgins EM, Fuller LC, Smith CH. Guidelines for the management of tinea capitis. British Association of Dermatologists. Br J Dermatol. 2000; 143:53-58. 

• 16. Ileana N, Adrian M. The fungal microbiota isolated from cats and dogs. J Anim Sci Biotechno. 2010; 43: 411-414.
• 17. Kanbe T. Molecular approaches in the diagnosis of dermatophytosis. Mycopathologia. 2008; 166:307-317.
• 18. Kristensen S, Krogh HV. A study of skin diseases in dogs and cat. VII. Ringworm infection . Nord Vet Med. 1981; 33: 134-140.
• 19. Lewis DT, Foil CS, Hosgood G. Epidemiology and Clinical Features of Dermatophytosis in Dogs and Cats at Louisiana State University: 1981-1990. Vet Dermatol 1991; 2:53-58.
• 20. Lima SR, da Silve WA, da Siveira MM, Da Silva Machado Neves RC, Dutra V, Sousa VRF. Isolation of dermatophytes from 50 asymptomatic domestic cats treated at the Federal University of Mato Grosso Veterinary – Hospital in Cuiabá, MT. Ciencias Agrarias. 2016; 37:2003-2008.
• 21. Mancianti F, Nardoni S, Cecchi S, Corazza M, Taccini F. Dermatophytes isolated from symptomatic dogs and cats in Tuscany, Italy during a 15-year period. Mycopathologia. 2002; 156:13-18.
• 22. Mackenzie DWR, Philpot CM. Isolation and identification of ringworm fungi. Public Health Laboratory Service. Monograph Series No 15. HM Stationary Office London, UK, 1981.
• 23. Moriello KA, Kunlke G, DeBoer DJ. Isolation of dermatophytes from the haircoats of stray cats from selected animal shelters in two different geographic regions in the United States. Vet Derm. 1994; 5:57-62.
• 24. Moriello KA. Treatment of dermatophytosis in dogs and cats: review of published studies. Vet Derm. 2004; 15, 99-107.
• 25. Mnelaos LA. Dermatophytosis in dog and cat. Buletin USAMV-CN. 2006; 63: 304-308.
• 26. Ogawa H, Summerbell RC, Clemons KV. Dermatophytes and host defence in cutaneous mycoses. Medical Mycology. 1998; 36: 166-173.
• 27. Panasiti V, Borroni RG, Devirgiliis V. Comparison of diagnostic methods in the diagnosis of dermatomycoses and onychomycoses. Mycoses. 2006; 49:26-29.
• 28. Proverbio D, Perego R, Spada E, de Giorgi GB, Pepa AD, Ferro E. Survey of Dermatophytes in Stray Cats with and without Skin Lesions in Northern Italy. Vet Med Int. 2014; 2014: 1-4.
• 29. Quinn PJ, Carter ME, Markey B, Carter GR. Clinical Veterinary Microbiology. p: 381-390. London, England: Mosby-Wolfe; 1999.
• 30. Robert R, Pihet M. Conventional methods for the diagnosis of dermatophytes. Mycopathologia. 2008; 166: 295-306. 

• 31. Roshanzamir H, Naserli S, Ziaie B, Fakour M. Incidence of dermatophytes isolated from dogs and cats in the city of Baku, Azerbaijan. Comp Clin Pathol
. 2016; 25:327-329.
• 32. Scott DW, Miller WH, Griffin CE., eds. Fungal skin diseases. In: Muller and Kirk’s Small Animal Dermatology, 6th ed. p: 336–361. Philadelphia: W.B. Saunders; 2001.
• 33. Seebacher C, Abeck D, Brasch J. Tinea capitis: ringworm of the scalp. Mycoses. 2007; 50:218-226.
• 34. Sparkes AH, Stokes GR, Gruffid-Jones TJ. Humoral immune response in cats with 
dermatophytosis. Am J of Vet Res. 1993; 54: 1869-1873. 
 35. Sparkes AH, Werret G, Stokes GR, Gruffydd-Jones TJ. Microsporum canis unapparent carriage by cats and the viability of arthospores. J Small Anim Pract. 1994; 35: 397- 401.
• 36. Stenwig H. Isolation of dermatophytes from domestic animals in Norway. Nord Vet Med. 1985; 37:161-169.
• 37. Seker E, Dogan N. Isolation of dermatophytes from dogs and cats with suspected dermatophytes in Western Turkey. Prev Vet Med. 2011; 98:46-51.
• 38. Tel OY, Akan M. Kedi ve köpeklerden dermatofitlerin izolasyonu. Ankara Univ Vet Fak Derg. 2008; 55:167–171.
• 39. Wright AI. Ringworm in dogs and cats. J Small Anim Pract. 1989; 30: 242-249.