Yenebilir / Tıbbi Önemi Olan Makrofunguslardan Ekstrakte Edilen Polisakkaritlerin in vitro Prebiyotik Aktivitesi

Öz

Bu çalışmada Agaricus bisporus (Kültür mantarı; beyaz ve kestane formu), Boletus edulis (Çörek mantarı), Cantharellus cibarius (Sarıkız mantarı), Ganoderma lucidum (Reyşi), Pleurotus ostreatus (İstiridye mantarı) ve Trametes versicolor (Hindi kuyruğu) türlerinden ekstrakte edilen polisakkaritler in vitro prebiyotik potansiyelleri açısından araştırılmıştır. Bu amaçla %0.25, %0.5, %1 ve %2 konsantrasyonlarda polisakkarit içeren ortamda Lactobacillus plantarum, Lactobacillus acidophilus ve Escherichia coli bakterilerinin üremesi 24 saat boyunca nefalometrik olarak belirlenmiştir. Trametes versicolor ve Ganoderma lucidum en yüksek (%5.59) ve en düşük (%0.31) polisakkarit verimi veren türler olmuştur. Yüksek polisakkarit konsantrasyonlarının tüm bakterilerin üremesini ihhibe ettiği görülmüştür. Agaricus bisporus (beyaz form), Trametes versicolor, Cantharellus cibarius, Boletus edulis ve Pleurotus ostreatus türlerinden elde edilen polisakkaritlerin potansiyel prebiyotik olarak kullanılabileceği belirlenmiştir.

Anahtar Kelimeler

• Makrofungus
• Prebiyotik
• Mikrobiyota
• Nefalometre
• In vitro

In vitro Prebiotic Activity of Polysaccharides Extracted from Edible / Medicinal Macrofungi Species

Öz

In this study, the extracted polysaccharides of Agaricus bisporus (Kültür mantarı; white and cream lines), Boletus edulis (Çörek mantarı), Cantharellus cibarius (Sarıkız mantarı), Ganoderma lucidum (Reyşi), Pleurotus ostreatus (İstiridye mantarı) and Trametes versicolor (Hindi kuyruğu) were investigated in terms of their in vitro prebiotic potential. For this purpose, the growth of Lactobacillus plantarum, Lactobacillus acidophilus and Escherichia coli were observed as nephalometrically in presence of polysaccharides at 0.25%, 0.5%, 1.0% and 2% concentrations for 24 hours. Trametes versicolor and Ganoderma lucidum have presented the highest (5.59%) and lowest (0.31%) polysaccharide yields, respectively. The higher polysaccharide concentrations have inhibited to proliferation of all bacterial strains. Among the polysaccharides, efficient ones as potential prebiotics were Agaricus bisporus (white line), Trametes versicolor, Cantharellus cibarius, Boletus edulis, and Pleurotus ostreatus.

Anahtar Kelimeler

• Macrofungi
• Prebiotic
• Microbiota
• Nephelometre
• In vitro

Kaynakça

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