FNDC5 Geni Polimorfizmleri, Serum İrisin Düzeyleri ve Alerjik Rinit Arasındaki İlişkinin Yokluğu

Yıl 2023, , 170 - 177, 29.10.2023

Durkadın Demir Ekşi , Hüseyin Günizi

https://doi.org/10.30565/medalanya.1341821

Öz

Amaç: Alerjik rinit (AR), alerjen maruziyetine bağlı olarak tip 1 immünoglobulin E-aracılı reaksiyonlarla oluşan bir enflamatuar burun mukozası hastalığıdır. İrisin, egzersize yanıt olarak iskelet kasları tarafından salınan bir hormondur. İrisin ile inflamasyon ilişkisini gösteren çalışmalar bulunmaktadır. Bu çalışmada, irisin kodlayan fibronektin tip III domain 5 (FNDC5) gen polimorfizmleri, serum irisin seviyeleri ve AR arasındaki potansiyel ilişkiyi araştırmayı amaçladık.

Yöntem: Çalışma, 100 AR hastası ve 100 sağlıklı kontrol bireyin yer aldığı bir vaka-kontrol çalışması şeklinde dizayn edilmiştir. FNDC5 geninde bulunan rs726344 ve rs1746661 SNP'lerin genotipleme analizleri PCR-RFLP yöntemiyle gerçekleştirilmiştir. Serum irisin seviyeleri ELISA yöntemi ile ölçülmüştür.

Sonuçlar: Hasta grubunda rs726344 SNP genotipleme sonuçlarına göre, %90 GG ve %10 GA genotipleri saptanırken, kontrol grubunda sırasıyla %94 GG ve %6 GA genotipleri belirlendi. AA genotipi hiçbir olguda saptanmadı. rs1746661 SNP için ise hastalarda %57 GG, %39 GT ve %4 TT genotipleri, kontrollerde ise sırasıyla %58 GG, %36 GT ve %6 TT genotipleri saptandı. Hastalar ile kontroller arasında rs726344 ve rs1746661 SNP'lerinde genotipik farklılık saptanmadı. Serum irisin seviyesi, hastalarda 406.3±56.09 ng/ml iken, kontrollerde 354.3±46.06 ng/ml olarak bulundu ve aralarında anlamlı farklılık görülmedi.

Sonuç: Bu çalışma, irisin proteininin, kodlayıcı geni ve AR arasındaki ilişkiyi araştırmayı amaçlayan ilk çalışmadır. FNDC5 gen polimorfizmleri, serum irisin seviyeleri ve AR arasında anlamlı bir ilişki saptanmamıştır. Bu bulgular, bu faktörlerin AR gelişiminde sınırlı bir rolünü göstermekle birlikte, irisin-AR ilişkisinin anlaşılması için ileri çalışmalara ihtiyaç bulunmaktadır.

Anahtar Kelimeler

Alerjik rinit, irisin, FNDC5, SNP, genotipleme.

Proje Numarası

2021-04-03-MAP12

Lack of Association Between FNDC5 Gene Polymorphisms, Serum Irisin Levels and Allergic Rhinitis

Öz

Aim: Allergic rhinitis (AR) is an inflammatory nasal mucosa disease caused by type 1 immunoglobulin E-mediated reactions to allergen exposure. Irisin is a hormone released by skeletal muscles in response to exercise. There are studies that demonstrate the relationship of irisin with inflammation. We aimed to investigate the potential association between irisin coding fibronectin type III domain 5 (FNDC5) gene polymorphisms, serum irisin levels, and AR.

Method: A case-control study was designed, involving 100 AR patients and 100 healthy controls. Genotyping of rs726344 and rs1746661 SNPs within the FNDC5 gene was performed using PCR-RFLP method. Serum irisin levels were measured using ELISA.

Results: Genotyping of rs726344 SNP in patients revealed 90% GG and 10% GA genotypes, while in controls, it was 94% GG and 6% GA. The AA genotype was not detected in any case. For rs1746661 SNP, patients had 57% GG, 39% GT, and 4% TT genotypes, while controls had 58% GG, 36% GT, and 6% TT genotypes. No significant difference was found in rs726344 and rs1746661 SNPs between the patients and the control group. Serum irisin level was 406.3±56.09 ng/ml in patients and 354.3±46.06 ng/ml in controls with no significant difference.

Conclusion: This is the first study aiming to investigate the relationship between the irisin protein, its encoding gene, and AR. No significant association was identified between FNDC5 gene polymorphisms, serum irisin levels, and allergic rhinitis. While these findings suggest a limited role of these factors in AR, further studies are needed for more comprehensive understanding of the irisin-AR relationship.

Anahtar Kelimeler

Allergic rhinitis, irisin, FNDC5, SNP, genotyping

Destekleyen Kurum

Scientific Research Projects Unit of Alanya Alaaddin Keykubat University

Proje Numarası

2021-04-03-MAP12

Teşekkür

The authors thank Dr. Yunus Emre Ekşi for his assistance in statistical analysis.

Kaynakça

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