Effects of LDD and CAPE administration on total antioxidant and total oxidant levels in experimental periodontitis model of rat brain

Yıl 2022, Cilt: 6 Sayı: 1, 107 - 113, 27.03.2022

Umut Yiğit , Fatma Yeşim Kırzıoğlu , Özlem Özmen , Abdülhadi Uğuz

https://doi.org/10.30565/medalanya.1052586

Öz

Aim: Observing the effects of caffeic acid phenethyl ester (CAPE) and/or low dose doxycycline (LDD) on total antioxidant and oxidant status of brain in experimental periodontitis is the purpose of the study.

Methods: 48 male Wistar albino rats were designed as the following: control group (C, n=8), periodontitis + CAPE group (PC, n=10), periodontitis + LDD (PD, n=10), periodontitis + LDD + CAPE group (PCD, n=10), and periodontitis group (P, n=10). The time period for the experiment was 14 days. 10µmol/kg/day of CAPE was administered using an intraperitoneal injection (IP). 10 mg/kg/day of LDD was administered using an oral gavage method. Histopathological changes were evaluated.

Results: Beneficial results were seen in all of the groups after LDD and/or CAPE administration on decreasing the alveolar bone loss level and oxidative stress. All of the experimental groups showed signs of periodontitis with alveolar bone loss. The P group leads with the most alveolar bone loss compared to the other periodontitis groups and the lowest group was the PC group in the periodontitis groups. The evolution of alveolar bone loss from high to low was that group P, group PD, group PCD, group PC, and group C (P < 0.05). However, there is no statistical difference between brain total antioxidant status and brain total oxidant status average values according to brain groups (p > 0.05).

Conclusion: The combination of LDD and CAPE are not significantly different when applied alone or together on oxidative status. But both of the agents have beneficial effects on reducing the oxidative stress and tissue damages. 

Anahtar Kelimeler

Oxidative stress, periodontitis, brain, total antioxidant status

Sıçan beyninin deneysel periodontitis modelinde DDD ve KAFE uygulamasının toplam antioksidan ve toplam oksidan düzeyleri üzerine etkileri

Öz

Amaç: Deneysel periodontitis modelinde kafeik asit fenetil ester (CAPE) ve/veya düşük doz doksisiklinin (LDD) beyin total antioksidan ve oksidanlar üzerine etkisinin araştırılmasıdır.

Yöntem: 48 adet erkek Wistar albino rat, kontrol grubu (C, n=8), periodontitis + CAPE grubu (PC, n=10), periodontitis + LDD (PD, n=10), periodontitis + LDD + CAPE (PCD, n=10) ve periodontitis grubu (P, n=10) şeklinde gruplara ayrıldı. Çalışma süresi 14 gün olarak belirlendi. 10µmol/kg/gün CAPE, bir intraperitoneal enjeksiyon (İP) kullanılarak,10 mg/kg/gün LDD, oral gavaj yöntemi kullanılarak uygulandı. Değişiklikler histopatolojik olarak değerlendirildi.

Bulgular: LDD ve/veya CAPE uygulamasının tüm gruplarda oksidatif stress ve alveolar kemik kaybı şiddetinin azaltması açısından faydalı sonuçlar gösterdiği belirlendi. Deney gruplarının tamamında periodontitisin kesin bulgusu olarak alveolar kemik kaybı tespit edildi. Sadece P grubunda, diğer periodontitis gruplarına kıyasla daha fazla alveoler kemik kaybı gözlenirken, PC grubunda ise en az alveolar kemik kaybı gözlendi. Gruplar arasında alveolar kemik kaybının yüksekten düşüğe sıralaması şu şekildeydi: grup P, grup PD, grup PCD, grup PC ve grup C (p < 0.05). Buna ragmen beyin gruplarına göre beyin total antioksidan durumu ve beyin total oksidan durumu ortalama değerleri arasında istatistiksel olarak anlamlı bir farklılık tespit edilmedi. (p > 0.05).

Sonuç: LDD ve CAPE kombinasyonu, tek başına veya birlikte uygulandığında oksidatif durum ve doku hasarı üzerine istatistiksel olarak herhangi bir anlamlı farklılık göstermedi. Ancak her iki ajanın da oksidatif stresi ve doku hasarlarını azaltmada faydalı etkileri vardır.

Anahtar Kelimeler

Oksidatif stres, periodontitis, beyin, toplam antioksidan durum

Kaynakça

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