Detection of complex vertebral malformation in holstein cattle reared in Antalya using allele-spesific PCR

Abstract

This study aimed to analyze complex vertebral malformation (CVM) genetic defect in Holstein raised in Antalya province using allele specific PCR (AS-PCR). The presence of CVM mutant allele was investigated in 200 Holstein cows raised by member breeders of Antalya Cattle Breeders Association and raised in Akdeniz University, Faculty of Agriculture. Total genomic DNA was extracted from blood samples obtained from Holstein cows. In the 395 bp length of SLC35A3 gene region of bovine chromosome 3, different primers were used to identify the normal and mutant allele. By using agarose gel electrophoresis, individuals were genotyped after AS-PCR. In this study, 7 of 200 individuals were found as CVM carriers.

Keywords

• CVM,SLC35A3 gene,Hereditary diseases

Project Number

2014.02.0121.016

Antalya’da yetiştirilen holstein sığırlarında kompleks vertebral malformasyon kalıtsal hastalığının allele özgü PCR ile belirlenmesi

Abstract

Bu çalışmada kalıtsal bir hastalık olan kompleks vertebral malformasyonun (CVM) Antalya ilinde yetiştiriciliği yapılan Siyah Alaca sığırlarında varlığı allele özgü polimeraz zincir reaksiyonu (AS-PCR) kullanılarak araştırılmıştır. Antalya Damızlık Sığır Yetiştiricileri Birliğine üye işletmelerden ve aynı birliğe üye olan Akdeniz Üniversitesi, Ziraat Fakültesi süt sığırcılığı işletmesine ait toplam 200 bireyde CVM kalıtsal hastalığının varlığı araştırılmıştır. Holstein sığırlarından elde edilen kan örneklerinden total genomik DNA izole edilmiştir. Sığır genomunun 3. kromozomu üzerinde bulunan SLC35A3 geninin 395 bç uzunluğundaki bölgesinde normal ve mutant allellerin belirlenmesinde farklı primerler kullanılmıştır. AS-PCR işleminden sonra uygulanan agaroz jel elektroforezi sonuçlarına göre bireyler genotiplendirilmiştir. Bu çalışmada; incelenen 200 bireyden 7 tanesinin CVM taşıyıcısı olduğu belirlenmiştir.

Keywords

• CVM,SLC35A3 geni,Kalıtsal hastalıklar

Supporting Institution

Akdeniz Üniversitesi Bilimsel Araştırma Projeleri Koordinasyon Birimi

Project Number

2014.02.0121.016

Thanks

Bu çalışma Akdeniz Üniversitesi Bilimsel Araştırma Projeleri Koordinasyon Birimi tarafından 2014.02.0121.016 nolu proje ile desteklenmiştir.

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