Deltamethrinin Beyinde Oluşturduğu Oksidatif Hasarın Önlenmesinde Funalia Trogii\'nin Rolü

Yıl 2010, Cilt: 3 Sayı: 1, - , 01.03.2010

Mehmet Berköz Serap Yalın Ülkü Çömelekoğlu Birgül Mazmancı Mehmet Ali Mazmancı Ali Ünyayar Pelin Eroğlu

Öz

Abstract The Role of Funalia trogii for Prevention of Oxidative Damage in Brain Tissue Induced by Deltamethrin Objective: Deltamethrin, a synthetic prethyroid material, has been widely used as an insecticide on the agricultural areas in the city of Mersin and its outskirts. Recently, medical plants and fungi have been used to avoid harmful effects of insecticides on the living organisms. In this study, the preventive effect of a medical fungus: Funalia trogii, on the oxidative damage in the brains of the rats that were subjected to deltamethrine was investigated. Method: In this study, 21 Wistar albino rats were used. Animals were divided into 3 groups with 7 rats in each group. Control group was not subjected to any procedure throughout the study. Deltamethrin group received 1.28 mg/kg deltamethrin over 30 day period whereas 1.28 mg/kg deltamethrin and 0.5 mL of Funalia trogii extract were administered to the deltamethrin+fungus group for the same period. In the end, all the animals were sacrificed. Antioxidant activities were determined in the brain tissues based on superoxide dismuthase and catalase enzyme levels. Also, malondialdehyde levels were measured for lipid peroxidation. Results: It was found that malondialdehyde levels of both the deltamethrin and the deltamethrin+fungus groups are higher than control group. Malondialdehyde level of the deltamethrin+fungus group was lower than that of the deltamethrin group. However, it wasn\'t statistically significant (p>0.05). Superoxide dismutase activity of the deltamethrin group was higher, but catalase activity was lower than control group. Superoxide dismutase activity of the deltamethrin+fungus group was lower. On the other hand, its catalase activity was higher than that of the deltamethrin group, statistically (p<0.05). Conclusion: Based on the data, it may be suggested that deltamethrin induces the lipid peroxidation. Treatment with Funalia trogii extract helps preventing oxidative stress by reducing lipid peroxidation caused by deltamethrin exposure. Thus, it can be stated that Funalia trogii could prevent lipid peroxidation in the brain tissue exposed to deltamethrin.

Anahtar Kelimeler

deltamethrin, Funalia trogii, lipid peroxidation, antioxidant system, brain tissue.

Deltamethrinin Beyinde Oluşturduğu Oksidatif Hasarın Önlenmesinde Funalia Trogii\'nin Rolü

Öz

Türkçe Özet: Amaç: Deltamethrin piretroid grubundan bir insektisit olup Mersin ili ve çevresi tarım alanlarında yaygın olarak kullanılmaktadır. Son dönemlerde insektisitlerin canlılar üzerindeki zararlı etkilerini gidermek için tıbbi bitki ve mantarlardan yararlanılmaktadır. Bu çalışmada bir tıbbi mantar olan Funalia trogii\'nin deltamethrine maruz kalmış sıçanların beyninde oluşabilecek oksidatif hasarın önlenmesinde etkili olup olmayacağı araştırılmıştır. Yöntem: Çalışmamızda toplam 21 Wistar albino sıçan kullanılmış ve her grupta 7 hayvan olacak şekilde 3 gruba ayrılmıştır. Kontrol grubuna deney boyunca hiçbir işlem uygulanmamıştır. Deltamethrin grubu 30 gün boyunca 1.28 mg/kg deltamethrin ve deltamethrin+mantar grubu ise 30 gün boyunca 1.28 mg/kg deltamethrin ve 0.5 mL Funalia trogii ekstresi almıştır. Çalışma sonunda tüm hayvanlar sakrifiye edilmiş ve beyin dokularında antioksidan aktivitenin tayini için süperoksit dismutaz ve katalaz enzimlerine, lipid peroksidasyonu belirlemek için ise malondialdehit düzeylerine bakılmıştır. Bulgular: Deltamethrin ve deltamethrin+mantar gruplarının malondialdehit düzeyleri kontrol grubuna kıyasla yüksek bulunmuştur. Deltamethrin+mantar grubunun malondialdehit düzeyi ise deltamethrin grubuna kıyasla düşük bulunmuş ancak bu düşüşün anlamlı olmadığı gözlenmiştir (p>0.05). Deltamethrin grubunun süperoksit dismutaz aktivitesi kontrole göre yüksek ve katalaz aktivitesi ise düşük bulunmuştur. Deltamethrin+mantar grubunun süperoksit dismutaz aktivitesi deltamethrin grubuna kıyasla istatistiksel olarak düşük, katalaz aktivitesi ise yüksek bulunmuştur (p<0.05). Sonuç: Bu verilere bağlı olarak deltamethrinin lipid peroksidasyonunu indüklediği söylenebilir. Funalia trogii ekstresinin uygulanması ise deltamethrin ile maruziyette lipid peroksidasyonunu azaltarak oksidatif stresin önlenmesine yardımcı olmaktadır. Sonuç olarak deltamethrinin beyin dokusunda oluşturduğu lipid peroksidasyonu önlemede, Funalia trogii\'nin olumlu etkisi olduğu düşünülebilir.

Anahtar Kelimeler

deltamethrin, Funalia trogii, lipid peroksidasyonu, antioksidan sistem, beyin dokusu.

Kaynakça

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