Cytotoxic and antioxidant effects of root extracts of Alchemilla mollis (Buser) Rothm

Yıl 2021, Cilt: 14 Sayı: 2, 176 - 185, 25.08.2021

Selen İlgün Gökçe Şeker Karatoprak

https://doi.org/10.26559/mersinsbd.796643

Öz

Aim: It is known that the herba of the species belonging to the genus of Alchemilla L. is used as a diuretic, astringent, antispasmodic, curative, cure in invalid menstruation and for convulsive purposes. It has been determined that they have antioxidant, antimicrobial and anti-inflammatory effects due to their rich polyphenol content. This study, it was aimed to determine the effectiveness of the extracts prepared from the roots of the A. mollis, one of the species belonging to the genus, by performing various activity tests to determine the antioxidant and cytotoxic effects. Method: In this study, determination of total phenol and flavonoid amount of methanol extract prepared from roots of A. mollis species and sub-extracts with different polarity obtained by fractionation of this extract and, determination of radical scavenging effects of DPPH and ABTS to obtain their antioxidant capacity was evaluated. Cytotoxic effects of these extracts in L929 and MDA-MB-231 cell lines were also evaluated. Result: High phenolic content and accordingly high antioxidant capacity were determined in the root extracts of the plant. According to the results obtained from the extracts obtained from plant roots in L929 healthy mouse fibroblast cells and MDA-MB-231 breast cancer cell line; The extracts were found to be non-toxic to healthy cells within a certain concentration range, but toxic to breast cancer cells at certain doses. Conclusion: As a result, it has been determined with our studies that the A. mollis plant can be an important source in the discovery of promising therapeutic agents in cancer treatment by inhibiting the proliferation of cancerous cells with its rich phenolic compound content.

Anahtar Kelimeler

Alchemilla, rosaceae, cytotoxicity, antioxidant capacity

Alchemilla mollis (Buser) Rothm bitkisi köklerinden elde edilen ekstrelerin antioksidan ve sitotoksik etkileri

Öz

Amaç: Alchemilla L. cinsine ait türlerin genellikle toprak üstü kısımlarının halk arasında diüretik, astrenjan, antispazmodik, yara iyileştirici, aşırı menstürasyonda kür olarak ve konvulsif hastalıkların tedavisinde kullanılmakta olduğu bilinmektedir. Sahip oldukları zengin polifenol içeriği sebebiyle de antioksidan, antimikrobiyal ve antienflamatuvar etkiler gösterdiği belirlenmiştir. Bu çalışmada ise cinse ait türlerden A. mollis bitkisinin köklerinden hazırlanan ekstrelerin antioksidan ve sitotoksik etkilerinin belirlenmesi için çeşitli aktivite testleri yapılarak, etkinliğinin tespit edilmesi amaçlanmıştır. Yöntem: Bu çalışmada A. mollis türünün köklerinden hazırlanan metanol ekstresi ve bu ekstrenin partisyonu ile elde edilen farklı polariteye sahip alt ekstrelerin toplam fenol ve flavonoit miktar tayini ve antioksidan kapasitelerini belirlemek için DPPH ve ABTS radikalini süpürücü etkileri tespit edilmiştir. Ayrıca ekstrelerin L929 ve MDA-MB-231 hücre hatlarında sitotoksik etkileri değerlendirilmiştir. Bulgular: Yüksek fenolik içeriğine bağlı olarak, bitkinin kök ekstrelerinde yüksek antioksidan kapasite tespit edilmiştir. Bitkinin köklerinden elde edilen ekstrelerin L929 sağlıklı fare fibroblast hücrelerinde ve MDA-MB-231 meme kanseri hücre hattında elde edilen sonuçlara göre; ekstreler belli bir konsantrasyon aralığında sağlıklı hücrelere toksik etki göstermezken meme kanseri hücreleri üzerinde belirli dozlarda toksik etkili bulunmuştur. Sonuç: Sonuç olarak A. mollis bitkisi zengin fenolik bileşik içeriği ile kanserli hücrelerin poliferasyonunu inhibe ederek kanser tedavisinde umut verici terapötik ajanların keşfedilmesinde önemli bir kaynak olabileceği tarafımızca yapılan çalışmalarla tespit edilmiştir

Anahtar Kelimeler

Alchemilla, Rosaceae, sitotoksisite, antioksidan kapasite

Kaynakça

• 1. Rashid S, Manzoor AR, Wajaht AS, Bilal AB. Chemical composition, antimicrobial, cytotoxic and antioxidant activities of the essential oil of Artemisia indica Willd. Food Chem 2013; 138: 693-700.
• 2. Demiroz T, Nalbantsoy A, Aydin Kose F, Baykan S. Phytochemical composition and antioxidant, cytotoxic and anti-inflammatory properties of Psephellus goeksunensis (Aytaç & H. Duman) Greuter & Raab-Straube. S. Afr. J. Bot 2020; 130: 1-7.
• 3. Ukwubile CA, EO Ikpefan, TS Malgwi, et al. Cytotoxic effects of new bioactive compounds isolated from a Nigerian anticancer plant Melastomastrum capitatum Fern. leaf extract. Scientific African 2020; 8: 421.
• 4. Ripa FA, Mahmuda H, Laizuman N, Islam M. Antibacterial, cytotoxic and antioxidant activity of Passiflora edulis Sims. Eur J Sci Res 2009; 31: 592-98.
• 5. Y Abdul-Hafeez E, Orabi AA M, HM Ibrahim O, Ilinskaya O, and S Karamova N. In vitro cytotoxic activity of certain succulent plants against human colon, breast and liver cancer cell lines. S. Afr. J. Bot 2020; 131: 295-301.
• 6. Kaya B, Menemen Y, Saltan ZF. Flavonoid Compounds Identified in Alchemilla L. Species Collected in the North-Eastern Black Sea Region of Turkey. Afr J Tradit Complement Altern Med 2012; 9(3): 418–425.
• 7. Hayirlioglu-Ayaz S, Inceer H. Three new Alchemilla L.(Rosaceae) records from Turkey, Pak J Bot 2009; 41: 2093-96. 8. Baytop T. Turkiye’de Bitkiler ile Tedavi. 2. baskı, İstanbul ,Nobel Tip Kitabevleri,1999:370.
• 9. Hamad I, Erol-Dayi Ö, Pekmez M, Onay-Ucar E, Arda N. Free radical scavenging activity and protective effects of Alchemilla vulgaris (L.). J. Biotechnol 2007; 2: 40-41.
• 10. Shrivastava R, Nathalie C, Gareth WJ. Effects of Alchemilla vulgaris and glycerine on epithelial and myofibroblast cell growth and cutaneous lesion healing in rats, Phytotherapy Research: An International Journal Devoted to Pharmacological and Toxicological Evaluation of Natural Product Derivatives 2007; 21: 369-73.
• 11. Falchero L, Mauro C, Alessia F, Giampiero L, Daniele R, Aldo T. Essential oil composition of lady's mantle (Alchemilla xanthochlora Rothm.) growing wild in Alpine pastures, Nat Prod Res 2009; 23: 1367-72.
• 12. Singleton Vernon L, Rudolf O, Rosa ML. Analysis of total phenols and other oxidation substrates and antioxidants by means of folin-ciocalteu reagent. Meth. Enzymol 1999; 14:152-178.
• 13. Miliauskas G, Venskutonis PR, Van Beek TA. Screening of radical scavenging activity of some medicinal and aromatic plant extracts, Food Chem 2004; 85: 231-37.
• 14. Gyamfi MA, Yonamine M, Aniya Y. Free-radical scavenging action of medicinal herbs from Ghana: Thonningia sanguinea on experimentally-induced liver injuries. Vasc Pharmacol 1999; 32: 661-67.
• 15. Re R, Nicoletta P, Anna P, Ananth P, Min Y, Catherine R. Antioxidant activity applying an improved ABTS radical cation decolorization assay. Free Radic. Biol. Med 1999; 26: 1231-37.
• 16. Boroja T, Mihailović V, Katanić J, Pan S-P, et al. The biological activities of roots and aerial parts of Alchemilla vulgaris L, S. Afr. J. Bot 2018; 116: 175-84.
• 17. Karatoprak GŞ, İlgün S, Koşar M. Antiradical, antimicrobial and cytotoxic activity evaluations of Alchemilla mollis (Buser) Rothm. Int. J. Herb. Med 2018; 6(2): 33-38.
• 18. Trendafilova A, Milka T, Milena N, Anna G, Antonina V. Flavonoid constituents and free radical scavenging activity of Alchemilla mollis. Nat Prod. Commun 2011; 6(11):1851-1854.
• 19. Karatoprak GŞ, İlgün S, Koşar M. Phenolic Composition, Anti‐Inflammatory, Antioxidant, and Antimicrobial Activities of Alchemilla mollis (Buser) Rothm, Chem. Biodivers 2017; 4:1-12.
• 20. Nedyalkov P, Maria K, Dasha M, Georgi K, Stefcho K. Influence of the ethanol concentration on the antioxidant capacity and polyphenol content of Alchemilla mollis extracts. Comptes rendus de l’Académie bulgare des Sciences 2015; 68: 1491-502.
• 21. Nohara K, Fang W, Sarah S. Glycosphingolipid composition of MDA-MB-231 and MCF-7 human breast cancer cell lines, Breast Cancer Res. Treat 1998; 48: 149-57.
• 22. Karabulut B. Üçlü-Negatif Meme Kanserli Hastalarda Güncel Klinik Uygulamalar ve Devam Eden Klinik Çalışmalar. Turkiye Klinikleri J Gen Surg-Special Topics 2013; 6(2):66-76.
• 23. Vlaisavljevi´c S, Jelaca S, Zengin G, et al. Alchemilla vulgaris agg. (Lady's mantle) from central Balkan: antioxidant, anticancer and enzyme inhibition properties. RSC Advances 2019; 9: 37474-4483.
• 24. Zarin MA, Wana HY, Ishab A, Armaniaa N. Antioxidant, antimicrobial and cytotoxic potential of condensed tannins from Leucaena leucocephala hybrid-RendangMazni. Food Science and Human Wellness 2016;5: 65–75.