Investigation of Germination and Early Seedling Development of Some Flax (Linum usitatissimum L.) Seeds Under Salt Stress
Yıl 2024,
Cilt: 14 Sayı: 1, 64 - 70, 28.06.2024
Şilan Çiçek Bayram
,
Nazlı Aybar Yalınkılıç
,
Sema Başbağ
Öz
Salinity, one of the abiotic stress factors, causes various physiological damages in plants. Salt stress significantly affects plant growth and development. One of the ways to reduce the negative effect of salt stress, which has a limiting effect on plant growth, is to determine the tolerance level of plants. This study was carried out under controlled conditions in order to examine the effect of salt stress on the germination of flax plant, which is one of the important industrial plants with various usage areas around the world. In this study, 3 flax varieties (Somme, Midin and Norman) and 4 salt concentrations (control, 50 mM, 100 mM and 200 mM) were used. In this experiment, germination rate, radicle length, plumula length, seedling length and seedling fresh weight characteristics of seeds under salt stress were examined. Statistically significant differences were detected between all the properties examined in the study, both among the cultivars and the salt doses. The study revealed that the control (pure water) group obtained the highest values in all the examined properties, but these values significantly decreased as the salinity severity increased. It was determined that the 200 mM salt dose significantly affected the germination of flax. ‘Somme’ flax variety was the most tolerant variety against salt doses in terms of the properties examined.
Etik Beyan
This research study complies with research and publishing ethics. The scientific and legal responsibility for manuscripts published in JAEFS belongs to the author(s).
Kaynakça
-
Abbasian, A., and J. Moemeni. (2013). Effects of salinity stress on seed germination and seedling vigor indices of
two halophytic plant species (Agropyron elongatum and A. pectiniforme). International Journal of Agriculture
and Crop Sciences, 5 (22):2669–76.
-
AbdulJaleel, C., R. Gopi, B. Sankar, P. Manivannan, A. Kishorekumar, R. Sridharan,and R. Panneerselvam. (2007).
Studies on germination, seedling vigour, lipid peroxidation and proline metabolism in Catharanthus roseus
seedlings under salt stress. South African Journal of Botany, 73:190–195. https://doi.org/10.1016/j.sajb.2006.11.001
-
Abido, W. A. E., & Zsombik, L. (2019). Effect of salinity on germination characters and seedlings parameters of
Egyptian flax cultivars growing in Nyiregyhaza. Acta Ecologica Sinica, 39(1), 102-108.
https://doi.org/10.1016/j.chnaes.2018.05.001
-
Almansouri, M., Kinet, J. M., & Lutts, S. (2001). Effect of salt and osmotic stresses on germination in durum wheat
(Triticum durum Desf.). Plant and Soil, 231, 243-254. https://doi.org/10.1023/A:1010378409663
-
Atak, M., Kaya, M. D., Kaya, G., Çikili, Y., & Çiftçi, C. Y. (2006). Effects of NaCl on the germination, seedling growth
and water uptake of triticale. Turkish Journal of Agriculture and Forestry, 30(1), 39-47.
-
Bayrak A, Kiralan M, Ipek A, Arslan N, Cosge B, Khawar KM. (2010). Fatty acid composition of linseed (Linum
usitatissimum L.) genotypes of different origin cultivated in Turkey. Biotechnology and Biotechnology
Equipment, 24:1836-1842. https://doi.org/10.2478/V10133-010-0034-2
-
Bilgili U., Budaklı Çarpıcı E., Aşık BB., Çelik N. (2011). Root and shoot response of common vetch (Vicia sativa L.),
forage pea (Pisum sativum L.) and canola (Brassica napus l.) to salt stress during early seedling growth
stages. Turkish Journal of Field Crops, 16(1): 33-38
-
Bojović, B., Đelić, G., Topuzović, M., & Stanković, M. (2010). Effects of NaCl on seed germination in some species
from families Brassicaceae and Solanaceae. Kragujevac Journal of Science, 32, 83-87.
-
Büyükyıldız, S., Yıldırım, M., & Kurt, A. N. (2023). The effect of salt stress on the germination and seedling growth
parameters in birdsfoot trefoil (Lotus corniculatus L.). Black Sea Journal Of Agriculture, 6(2), 126-133.
https://doi.org/10.47115/bsagriculture.1173277
-
Cloutier, S., Z. Niu, R. Datla, and S. Duguid. (2009). Development and analysis of EST-SSRs for flax (Linum
usitatissimum L.). Theoretical and Applied Genetics, 119:53–63. https://doi.org/10.1007/s00122-009-1016-3
-
Dubey, S., Bhargava, A., Fuentes, F., Shukla, S., & Srivastava, S. (2020). Effect of salinity stress on yield and quality
parameters in flax (Linum usitatissimum L.). Notulae Botanicae Horti Agrobotanici Cluj-Napoca, 48(2), 954-
966. https://doi.org/10.15835/nbha48211861
-
El-Nakhlawy, F.S. and M.A. El-Fawal. (1989). Tolerance of five oil crops to salinity and temperature stresses,
during germination. Acta Agronomica Hungarica, 38(1-2): 59-65.
-
Goyal A, Sharma V, Sihag MK, Singh AK, Arora S, Sabikhi L. (2016). Fortification of dahi (Indian yoghurt) with
omega-3 fatty acids using microencapsulated flaxseed oil microcapsules. Journal of Food Science and
Technology, 53:2422-2433. https://doi.org/10.1007/s13197-016-2220-1
-
ISTA. (1984). Association of Official Seed Analysts, Rules for Testing Seeds. J Seed Tech, 6: 1-125.
-
Jamil, M., D. B. Lee, K. Y. Jung, M. Ashraf, S. Ch. Lee, and E. Shik Rha. (2006). Effect of salt (NaCl) stress on
germination and early seedling growth of four vegetables species. Journal of Central European Agriculture
(JCEA), 2:273–82.
-
Kadkhodaıe, A., & Bagherı, M. (2012). Seed treatment during germination in linseed (Linum usitatissimum L.) to
overcome salt and drought stresses. World Academy of Science, Engineering and Technology International
Journal of Agricultural and Biosystems Engineering, 5(1): 12-16.
-
Kajla P, Sharma A, Sood DR (2015). Flaxseed- a potential functional food source. Journal of Food Science and
Technology, 52 (4):1857-1871. https://doi.org/10.1007/s13197-014-1293-y
-
Kaya D. M., Day, S., Cikili, Y., & Arslan, N. (2012). Classification of some linseed (Linum usitatissimum L.) genotypes
for salinity tolerance using germination, seedling growth, and ion content. Chilean Journal of Agricultural
Research, 72(1), 27-32. http://dx.doi.org/10.4067/S0718-58392012000100005
-
Maquire JD, (1962). Speed of germination aid in selection and evaluation for seedling emergence and vigor.
Crop Science, 2: 176- 177. http://dx.doi.org/10.2135/cropsci1962.0011183X000200020033x
-
Misra, N., & Dwivedi, U. N. (2004). Genotypic difference in salinity tolerance of green gram cultivars. Plant
Science, 166(5), 1135-1142. https://doi.org/10.1016/j.plantsci.2003.11.028
-
Moghaddam, M., Babaei, K., & Saeedi Pooya, E. (2018). Germination and growth response of flax (Linum
usitatissimum L.) to salinity stress by different salt types and concentrations. Journal of Plant Nutrition, 41(5),
563-573. https://doi.org/10.1080/01904167.2017.1392573
-
Mohammadizad, H. A., I. Khazaei, M. Ghafari, M. F. F. Sinehsar, and R. Roghaieh Barzegar. (2013). Effect of salt and
drought stresses on seed germination and early seedling growth of Nepeta persica. International Journal of
Farming and Allied Sciences, 2 (21):895–9.
-
Mostafavi K. (2012) Effect of salt stress on germination and early seedling growth stage of sugar beet cultivars.
American-Eurasian Journal of Sustainable Agriculture. 6(2): 120-125.
-
Muhammad, Z., & Hussain, F. (2010). Effect of NaCl salinity on the germination and seedling growth of some
medicinal plants. Pak. J. Bot, 42(2), 889-897.
-
Rajabi, R., and K. Postini. (2005). Effect of NaCl on thirty cultivars of bread wheat seed germination. Journal of
Agricultural Science, 27 (1):29–45.
-
Sadeghi, H., & Robati, Z. (2015). Response of Cichorium intybus L. to eight seed priming methods under osmotic
stress conditions. Biocatalysis and Agricultural Biotechnology, 4(4), 443-448.
https://doi.org/10.1016/j.bcab.2015.08.00
-
Tahjib-Ul-Arif, M., Siddiqui, M. N., Sohag, A. A. M., Sakil, M. A., Rahman, M. M., Polash, M. A. S., & Tran, L. S. P. (2018).
Salicylic acid-mediated enhancement of photosynthesis attributes and antioxidant capacity contributes to
yield improvement of maize plants under salt stress. Journal of Plant Growth Regulation, 37, 1318-1330.
https://doi.org/10.1007/s00344-018-9867-y
-
Vicente, M. J., E. Conesa, J. Lvarez-Rogel, J. A. Franco, and J. J. Martínez-Sánchez. (2007). Effects of various salts
on the germination of three perennial salt marsh species. Aquatic Botany, 87:167–70.
https://doi.org/10.1016/j.aquabot.2007.04.004
-
Wang, J., Lu, H., Chen, G. H., Lau, G. N., Tsang, W. L., & van Loosdrecht, M. C. (2009). A novel sulfate reduction,
autotrophic denitrification, nitrification integrated (SANI) process for saline wastewater treatment. Water
Research, 43(9), 2363-2372. https://doi.org/10.1016/j.watres.2009.02.037
-
Yaver, S., & Pasa, C. (2009). Application of different NaCl concentrations on seed germination of flax (Linum
usitatissimum L.) cultivar. Agricultural Science and Technology, 1(3), 103-105.
-
Zaghdoudi, M., Baatour, O., Bensalem, N., Ouerghi Abidi, Z. (2015): Effect of saline conditions on germination and
enzymatic activity in two varieties of Linum usitatissimum seeds. – Journal of New Sciences, Agriculture and
Biotechnology, 17: 629-638.
Tuz Stresine Maruz Bırakılan Bazı Keten (Linum usitatissimum L.) Tohumlarının Çimlenme ve Erken Fide Gelişiminin Araştırılması
Yıl 2024,
Cilt: 14 Sayı: 1, 64 - 70, 28.06.2024
Şilan Çiçek Bayram
,
Nazlı Aybar Yalınkılıç
,
Sema Başbağ
Öz
Abiyotik stres faktörlerinden biri olan tuzluluk, bitkilerde birçok fizyolojik hasarlara yol açmaktadır. Tuz stresi bitki büyüme ve gelişmesinde önemli bir rol oynar. Bitki gelişimini sınırlayıcı etkiye sahip olan tuz stresinin olumsuz etkisini azaltmanın yollarından biri de bitkilerin tolerans düzeyinin belirlenmesidir. Bu çalışma, dünyada birçok kullanım alanına sahip önemli endüstri bitkilerinden biri olan keten bitkisinin tuz stresi altında çimlenme ve fide gelişimi üzerine olan etkisini incelemek amacıyla kontrollü şartlar altında yürütülmüştür. Çalışmada 3 adet keten çeşidi (Somme, Midin ve Norman) ve 4 adet tuz konsatrasyonu (kontrol, 50 mM, 100 mM ve 200 mM) kullanılmıştır. Araştırmada tuz stresine maruz bırakılan tohumların çimlenme oranı, radikula uzunluğu, plumula uzunluğu, fide boyu ve fide yaş ağırlığı parametreleri incelenmiştir. Araştırmada incelenen tüm özelliklerde hem çeşitler hem de tuz dozları arasında istatistiksel olarak önemli farklılıklar tespit edilmiştir. Çalışma sonucunda incelenen tüm özelliklerde en yüksek değerler kontrol (saf su) grubundan elde edilirken, tuzluluk şiddeti arttıkça bütün değerlerde düşüşler meydana gelmiştir. Ayrıca, 200 mM tuzluluk dozunun keten çimlenmesini önemli ölçüde etkilediği çalışmanın sonuçları arasında yer almaktadır. İncelenen özellikler açısından 'Somme' keten çeşidi tuz dozlarına en toleranslı çeşit olmuştur.
Kaynakça
-
Abbasian, A., and J. Moemeni. (2013). Effects of salinity stress on seed germination and seedling vigor indices of
two halophytic plant species (Agropyron elongatum and A. pectiniforme). International Journal of Agriculture
and Crop Sciences, 5 (22):2669–76.
-
AbdulJaleel, C., R. Gopi, B. Sankar, P. Manivannan, A. Kishorekumar, R. Sridharan,and R. Panneerselvam. (2007).
Studies on germination, seedling vigour, lipid peroxidation and proline metabolism in Catharanthus roseus
seedlings under salt stress. South African Journal of Botany, 73:190–195. https://doi.org/10.1016/j.sajb.2006.11.001
-
Abido, W. A. E., & Zsombik, L. (2019). Effect of salinity on germination characters and seedlings parameters of
Egyptian flax cultivars growing in Nyiregyhaza. Acta Ecologica Sinica, 39(1), 102-108.
https://doi.org/10.1016/j.chnaes.2018.05.001
-
Almansouri, M., Kinet, J. M., & Lutts, S. (2001). Effect of salt and osmotic stresses on germination in durum wheat
(Triticum durum Desf.). Plant and Soil, 231, 243-254. https://doi.org/10.1023/A:1010378409663
-
Atak, M., Kaya, M. D., Kaya, G., Çikili, Y., & Çiftçi, C. Y. (2006). Effects of NaCl on the germination, seedling growth
and water uptake of triticale. Turkish Journal of Agriculture and Forestry, 30(1), 39-47.
-
Bayrak A, Kiralan M, Ipek A, Arslan N, Cosge B, Khawar KM. (2010). Fatty acid composition of linseed (Linum
usitatissimum L.) genotypes of different origin cultivated in Turkey. Biotechnology and Biotechnology
Equipment, 24:1836-1842. https://doi.org/10.2478/V10133-010-0034-2
-
Bilgili U., Budaklı Çarpıcı E., Aşık BB., Çelik N. (2011). Root and shoot response of common vetch (Vicia sativa L.),
forage pea (Pisum sativum L.) and canola (Brassica napus l.) to salt stress during early seedling growth
stages. Turkish Journal of Field Crops, 16(1): 33-38
-
Bojović, B., Đelić, G., Topuzović, M., & Stanković, M. (2010). Effects of NaCl on seed germination in some species
from families Brassicaceae and Solanaceae. Kragujevac Journal of Science, 32, 83-87.
-
Büyükyıldız, S., Yıldırım, M., & Kurt, A. N. (2023). The effect of salt stress on the germination and seedling growth
parameters in birdsfoot trefoil (Lotus corniculatus L.). Black Sea Journal Of Agriculture, 6(2), 126-133.
https://doi.org/10.47115/bsagriculture.1173277
-
Cloutier, S., Z. Niu, R. Datla, and S. Duguid. (2009). Development and analysis of EST-SSRs for flax (Linum
usitatissimum L.). Theoretical and Applied Genetics, 119:53–63. https://doi.org/10.1007/s00122-009-1016-3
-
Dubey, S., Bhargava, A., Fuentes, F., Shukla, S., & Srivastava, S. (2020). Effect of salinity stress on yield and quality
parameters in flax (Linum usitatissimum L.). Notulae Botanicae Horti Agrobotanici Cluj-Napoca, 48(2), 954-
966. https://doi.org/10.15835/nbha48211861
-
El-Nakhlawy, F.S. and M.A. El-Fawal. (1989). Tolerance of five oil crops to salinity and temperature stresses,
during germination. Acta Agronomica Hungarica, 38(1-2): 59-65.
-
Goyal A, Sharma V, Sihag MK, Singh AK, Arora S, Sabikhi L. (2016). Fortification of dahi (Indian yoghurt) with
omega-3 fatty acids using microencapsulated flaxseed oil microcapsules. Journal of Food Science and
Technology, 53:2422-2433. https://doi.org/10.1007/s13197-016-2220-1
-
ISTA. (1984). Association of Official Seed Analysts, Rules for Testing Seeds. J Seed Tech, 6: 1-125.
-
Jamil, M., D. B. Lee, K. Y. Jung, M. Ashraf, S. Ch. Lee, and E. Shik Rha. (2006). Effect of salt (NaCl) stress on
germination and early seedling growth of four vegetables species. Journal of Central European Agriculture
(JCEA), 2:273–82.
-
Kadkhodaıe, A., & Bagherı, M. (2012). Seed treatment during germination in linseed (Linum usitatissimum L.) to
overcome salt and drought stresses. World Academy of Science, Engineering and Technology International
Journal of Agricultural and Biosystems Engineering, 5(1): 12-16.
-
Kajla P, Sharma A, Sood DR (2015). Flaxseed- a potential functional food source. Journal of Food Science and
Technology, 52 (4):1857-1871. https://doi.org/10.1007/s13197-014-1293-y
-
Kaya D. M., Day, S., Cikili, Y., & Arslan, N. (2012). Classification of some linseed (Linum usitatissimum L.) genotypes
for salinity tolerance using germination, seedling growth, and ion content. Chilean Journal of Agricultural
Research, 72(1), 27-32. http://dx.doi.org/10.4067/S0718-58392012000100005
-
Maquire JD, (1962). Speed of germination aid in selection and evaluation for seedling emergence and vigor.
Crop Science, 2: 176- 177. http://dx.doi.org/10.2135/cropsci1962.0011183X000200020033x
-
Misra, N., & Dwivedi, U. N. (2004). Genotypic difference in salinity tolerance of green gram cultivars. Plant
Science, 166(5), 1135-1142. https://doi.org/10.1016/j.plantsci.2003.11.028
-
Moghaddam, M., Babaei, K., & Saeedi Pooya, E. (2018). Germination and growth response of flax (Linum
usitatissimum L.) to salinity stress by different salt types and concentrations. Journal of Plant Nutrition, 41(5),
563-573. https://doi.org/10.1080/01904167.2017.1392573
-
Mohammadizad, H. A., I. Khazaei, M. Ghafari, M. F. F. Sinehsar, and R. Roghaieh Barzegar. (2013). Effect of salt and
drought stresses on seed germination and early seedling growth of Nepeta persica. International Journal of
Farming and Allied Sciences, 2 (21):895–9.
-
Mostafavi K. (2012) Effect of salt stress on germination and early seedling growth stage of sugar beet cultivars.
American-Eurasian Journal of Sustainable Agriculture. 6(2): 120-125.
-
Muhammad, Z., & Hussain, F. (2010). Effect of NaCl salinity on the germination and seedling growth of some
medicinal plants. Pak. J. Bot, 42(2), 889-897.
-
Rajabi, R., and K. Postini. (2005). Effect of NaCl on thirty cultivars of bread wheat seed germination. Journal of
Agricultural Science, 27 (1):29–45.
-
Sadeghi, H., & Robati, Z. (2015). Response of Cichorium intybus L. to eight seed priming methods under osmotic
stress conditions. Biocatalysis and Agricultural Biotechnology, 4(4), 443-448.
https://doi.org/10.1016/j.bcab.2015.08.00
-
Tahjib-Ul-Arif, M., Siddiqui, M. N., Sohag, A. A. M., Sakil, M. A., Rahman, M. M., Polash, M. A. S., & Tran, L. S. P. (2018).
Salicylic acid-mediated enhancement of photosynthesis attributes and antioxidant capacity contributes to
yield improvement of maize plants under salt stress. Journal of Plant Growth Regulation, 37, 1318-1330.
https://doi.org/10.1007/s00344-018-9867-y
-
Vicente, M. J., E. Conesa, J. Lvarez-Rogel, J. A. Franco, and J. J. Martínez-Sánchez. (2007). Effects of various salts
on the germination of three perennial salt marsh species. Aquatic Botany, 87:167–70.
https://doi.org/10.1016/j.aquabot.2007.04.004
-
Wang, J., Lu, H., Chen, G. H., Lau, G. N., Tsang, W. L., & van Loosdrecht, M. C. (2009). A novel sulfate reduction,
autotrophic denitrification, nitrification integrated (SANI) process for saline wastewater treatment. Water
Research, 43(9), 2363-2372. https://doi.org/10.1016/j.watres.2009.02.037
-
Yaver, S., & Pasa, C. (2009). Application of different NaCl concentrations on seed germination of flax (Linum
usitatissimum L.) cultivar. Agricultural Science and Technology, 1(3), 103-105.
-
Zaghdoudi, M., Baatour, O., Bensalem, N., Ouerghi Abidi, Z. (2015): Effect of saline conditions on germination and
enzymatic activity in two varieties of Linum usitatissimum seeds. – Journal of New Sciences, Agriculture and
Biotechnology, 17: 629-638.