Ratlarda BPA ile Oluşturulan Böbrek Hasarında Ellajik Asit Uygulamasının DNA Hasarı ve Bazı Böbrek Fonksiyon Parametreleri Üzerine Etkisi

Öz

Amaç: Bisfenoller (BP'ler), genel popülasyonun yaygın olarak kullandığı birçok üründe bulunan potansiyel hormon bozuculardır. Bisfenol A (BPA) ise, vücudun metabolik sistemleri üzerindeki olumsuz etkilerine rağmen, bazı plastik ve reçinelerin üretiminde uzun yıllardır kullanılan endüstriyel bir kimyasaldır. BPA gibi toksik ajanlardan ve dolayısıyla çevresel kirleticilerden etkilenen en önemli organlardan biri böbreklerdir. Ellajik asit (EA) fitokimyasal bir polifenol bileşiğidir ve yapılan birçok çalışmada EA’nın antioksidan, anti-mutajenik, anti-kanser ve anti-apoptotik özellikler sergilediği rapor edilmektedir. Bu çalışmanın amacı BPA ile indüklenen böbrek hasarı üzerine EA’nın etkisini ortaya koymaktır. Gereç ve Yöntemler: Bu çalışmada 24 adet erkek rat, Kontrol, BPA (30 gün süreyle 25mg/kg), EA (30 gün süreyle 10 mg/kg) ve BPA+EA (30 gün süreyle sırasıyla 25mg-10mg/kg) olarak dört gruba ayrıldı (n=6). Çalışma sonunda dekapite edilen ratlardan alınan böbreklerin ağırlıkları ile kan serumundan kan üre nitrojeni (BUN) ve kreatinin değerleri ölçüldü. Bunun yanında böbreklerin genel histolojik görüntüsünü ortaya koymak amacıyla Pikro Sirius Red boyaması, dokularda meydana gelen DNA hasarının ortaya konulabilmesi amacıyla 8-hidroksideoksiguanozin antikoru kullanılarak immunohistokimyasal boyama yapılmıştır. Bulgular: Yapılan değerlendirme sonucunda sol böbreğe ait mutlak ve nispi ağırlığın BPA grubunda artış gösterdiği, BPA+EA grubunda ise kontrole yaklaşarak düştüğü gözlenmiştir. Kan üre azotu (BUN) ve kreatinin değerlerinde BPA grubunda kontrol grubuna göre ciddi artış olduğu görülmüş, BPA+EA grubunda ise bu değerlerin azaldığı tespit edilmiştir. BPA kullanılan gruptan alınan böbrek dokularında 8-hidroksideoksiguanozin primer antikorunun glomerulus ve tubul yapılarında yoğun pozitif reaksiyon verdiği, glomerulus yapısında dejenerasyon ve vakuolizasyon olduğu, bazı bölgesel nekroz alanları bulunduğu ve tubul yapılarının bozulduğu gözlenmiştir. Buna karşın EA ve kontrol gruplarında birbirine benzer şekilde oldukça az 8-hidroksideoksiguanozin pozitif alana rastlanmıştır. BPA ve EA’nın birlikte kullanıldığı grupta ise gerek glomerulus yapısı gerek tubul yapılarında BPA’nın tek başına kullanıldığı gruba göre düzelmeler görülürken 8- hidroksideoksiguanozin pozitif alanların azaldığı gözlenmiştir. Sonuç: Toplumun her kesiminden bireylerin maruz kalma ihtimali olan BPA’nın böbrek dokusuna vereceği muhtemel zararların, EA içeren gıda katkı maddeleri kullanılarak azaltılabileceği sonucuna varılmıştır.

Anahtar Kelimeler

• Böbrek
• bisfenol A
• BUN
• ellajik asit
• Kreatinin
• 8-hidroksideoksiguanozin

Destekleyen Kurum

Bu araştırma kamu, ticari veya kar amacı gütmeyen sektörlerdeki finansman kuruluşlarından herhangi bir özel hibe almamıştır.

Etik Beyan

Makalemiz ile ilgili herhangi bir kurum, kuruluş, kişi ile mali çıkar çatışması yoktur ve yazarlar arasında çıkar çatışması bulunmamaktadır.

Teşekkür

Laboratuvar teknik desteği için Araş. Gör. Tansu KUŞAT’a teşekkürlerimizi sunarız.

Ellagic Acid Administration on DNA Damage and Some Kidney Function Parameters in BPA-Induced Kidney Damage in Rats

Öz

Aim: Bisphenols (BPs) are potential hormone disruptors which the general population is exposed in many commonly used products. Bisphenol A (BPA) is an industrial chemical that has been used for many years in the production of some plastics and resins, despite its adverse effects on the body's metabolic systems. One of the most important organs affected by toxic agents such as BPA and also from environmental pollutants is the kidneys. Ellagic acid (EA) is a phytochemical polyphenol compound, and many studies report that EA exhibits antioxidant, anti-mutagenic, anti-cancer and anti-apoptotic properties. The aim of this study is to reveal the effect of EA on BPA-induced kidney damage. Material and Methods: In this study, 24 male rats were divided into four groups as Control, BPA (25mg/kg for 30 days), EA (10/mg/kg for 30 days) and BPA+EA (25mg-10mg/kg for 30 days, respectively) (n=6). At the end of the study, the weights of kidneys taken from decapitated rats and blood urea nitrogen (BUN) and creatinine values were measured from the serum. In addition, Picro Sirius Red staining was performed to reveal the general histological appearance of the kidneys, and immunohistochemical staining was performed using 8-hydroxydeoxyguanosine antibody to reveal the DNA damage occurring in the tissues. Results: It was observed that the absolute and relative weights of the left kidney increased in the groups where BPA was induced, and decreased in the BPA+EA group, similar to the control group. It was determined that blood urea nitrogen (BUN) and creatinine values increased significantly in the BPA group compared to the control group, while the values decreased in the BPA+EA group. As a result of staining with 8-hydroxydeoxyguanosine antibody in the glomerulus and tubule structures of the kidney tissues taken from the BPA-induced group, intense positivity, degeneration and vacuolization in the glomerulus structure, some regional necrosis areas and deterioration of the tubule structures were observed, while very few stained areas were observed in the EA and control groups, similar to each other. In the group where BPA and EA were used together, protection of the tissues was observed in both the glomerulus structure and tubule structures compared to the group where BPA was used alone, and less retention of 8-hydroxydeoxyguanosine was observed. Conclusion: It has been concluded that the possible damage to kidney tissue caused by BPA, which individuals from all segments of society may be exposed to, can be reduced by using EA supplements.

Anahtar Kelimeler

• Kidney
• bisphenol A
• BUN
• ellagic acid
• creatinine
• 8-hydroxy deoxyguanosine

Kaynakça

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