Determination of Phenolic Content by HPLC, Antimicrobial, Antioxidant and DNA Protective Activities of Nepeta transcaucasica Grossh. (kaf pisikotu) Extract

Öz

Members of the Lamiaceae family are widely used in traditional medicine and agriculture thanks to their secondary metabolites. In this study, the phenolic substance content of ethanol extract obtained from Nepeta transcaucasica Grossh. (kaf pisikotu) plant collected in Bitlis province was determined by HPLC and antimicrobial, antioxidant and DNA protective activities were investigated. It was determined that the extract contained the most apigenin and vanillin. When evaluated according to the antimicrobial activity results, it was observed that it showed good activity. It was also found that the extract showed better antibacterial activity than other antibiotics except erythromycin. Four different methods were used to determine the in vitro antioxidant properties of the extract. According to the results of the study, it was determined that N. transcaucasica extract exhibited antioxidant activity close to BHA, BHT and α-tocopherol standard antioxidants. Finally, it was observed that the extract had no protective effect on the pBR322 DNA plasmid. Since the studies on N. transcaucasica are limited, it is thought that the results obtained in this study will make important contributions to the literature.

Anahtar Kelimeler

• Antimicrobial
• antioxidant
• DNA
• HPLC
• Plant

Nepeta transcaucasica Grossh. (kaf pisikotu) Estraktının HPLC ile Fenolik Madde İçeriğinin Tayini, Antimikrobiyal, Antioksidan ve DNA Koruyucu Aktivitelerinin Belirlenmesi

Öz

Lamiaceae familyasının üyeleri içerdikleri sekonder metabolitler sayesinde geleneksel tıpta ve tarımda yaygın bir şekilde kullanılmaktadırlar. Bu çalışmada, Bitlis İlinde toplanan Nepeta transcaucasica Grossh. (kaf pisikotu) bitkisinden elde edilen etanol ekstraktının fenolik madde içeriği HPLC ile belirlenerek, antimikrobiyal, antioksidan ve DNA koruyucu aktiviteleri araştırıldı. Ekstraktın en fazla apigenin ve vanillin içerdiği belirlendi. Antimikrobiyal aktivite sonuçlarına göre genel olarak değerlendirildiğinde iyi derecede aktivite gösterdiği gözlemlendi. Aynı zamanda ekstraktın eritromisin hariç diğer antibiyotiklerden daha iyi antibakteriyal aktivite gösterdiği düşünülmektedir. Çalışmada, ekstraktın in vitro antioksidan özelliklerini belirlemek amacıyla dört farklı metot kullanıldı. Çalışmanın sonucuna göre N. transcaucasica ekstraktının, BHA, BHT ve α-tokoferol standart antioksidanlarına yakın antioksidan aktivite sergilediği belirlendi. Son olarak ekstraktın, pBR322 DNA plazmiti üzerine koruyucu etki göstermediği gözlendi. N. transcaucasica ile ilgili çalışmalar kısıtlı olduğundan, bu çalışmada elde edilen sonuçların literatüre önemli katkılar sunacağı düşünülmektedir.

Anahtar Kelimeler

• antimikrobiyal
• antioksidan
• Bitki
• DNA
• HPLC

Kaynakça

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