Vincetoxicum canescens subsp. canescens ve Vincetoxicum cancescens subsp. pedunculata Tohumlarının Antimikrobiyal ve Antiproliferatif Aktiviteleri

Yıl 2019, , 367 - 375, 25.09.2019

Sevda Güzel , Mahmut Ülger , Yusuf Özay , Önder Yumrutaş , İbrahim Bozgeyik Özkan Sarıkaya

https://doi.org/10.31020/mutftd.594212

Cited By: 3

Öz

Vincetoxicum cinsinin tıbbi özellikleri uzun zamandır bilinmektedir.
Bazı Vincetoxicum türlerinin
yaprakları, kuru tohumları ve rizomları geleneksel tıpta incinme, uyuz, nevroz,
skrofula, sıtma, yara, ateş, rüptür ve eksternal kanserlerin tedavisinde
kullanılmaktadır. Bu çalışma, V.
canescens subsp. canescens ve V. cancescens subsp. pedunculata (endemik) tohumlarının
anti(miko)bakteriyel, antifungal ve antiproliferatif aktivitelerini
değerlendirmeye odaklanmıştır. İki bitkinin tohumlarının etanol ve hekzan
ekstreleri üç fungal suşa (Candida albicans,
Candida tropicalis ve Candida glabrata), iki Gram-pozitif bakteri
suşuna (Bacillus subtilis ve
Staphylococcus aureus), üç Gram-negatif bakteri suşuna (Aeromonas hydrophila, Escherichia coli ve
Acinetobacter baumannii) ve Mycobacterium tuberculosis H37Rv suşuna
karşı Sıvı Mikrodilüsyon Yöntemi kullanılarak test edilmiştir. Referans ilaçlar
olarak Flukonazol, Etambutol, Ampisilin ve İzoniazid kullanılmıştır. Etanol ve
hekzan ekstrelerinin farklı konsantrasyonlarının (200, 100, 50 ve 25 μg/mL)
antiproliferatif etkileri MTT testi kullanılarak A549 insan akciğer kanseri
hücre dizilerine karşı test edilmiştir. Etanol ekstrelerinin A. baumannii'ye (62.5 μg/mL MİK değeri)
karşı Ampisilin'den (125 μg/mL MİK değeri) daha etkili olduğu bulunmuştur. A549
insan akciğer kanseri hücre dizilerine karşı V. canescens subsp. canescens
tohumlarının etanol ekstresinin 100 µg/mL konsantrasyonu ve V. cancescens subsp. pedunculata tohumlarının etanol ekstresinin
200 μg/mL konsantrasyonu, kontrol gruplarına göre istatistiksel olarak daha
düşük hücre canlılık seviyeleri sergilemiştir (P <0.05). Sonuç olarak, V. canescens subsp. canescens ve V. cancescens
subsp. pedunculata tohumları, test
edilen tüm mikroorganizmalara karşı antimikrobiyal ve A549 insan akciğer
kanseri hücre dizilerine karşı antiproliferatif etki göstermiştir; ayrıca,
etanol ekstreleri hekzan ekstrelerinden daha etkili bulunmuştur.

Anahtar Kelimeler

Vincetoxicum, Tohum, Antimikrobiyal

Destekleyen Kurum

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Proje Numarası

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Teşekkür

Bitki örneklerinin teşhisinde katkılarından dolayı Dr. Ahmet İlçim'e (Biyoloji Bölümü, Fen-Edebiyat Fakültesi, Mustafa Kemal Üniversitesi) teşekkür ederiz.

Antimicrobial and Antiproliferative Activities of Vincetoxicum canescens subsp. canescens and Vincetoxicum cancescens subsp. pedunculata Seeds

Öz

Medicinal
properties of Vincetoxicum genus have
been known for a long time. Leaves, dry seeds, and rhizomes of some Vincetoxicum species have been used in
folk medicine for the treatment of injuries, scabies, neurosis, scrofula,
malaria, wound, fever, rupture, and external cancers. The present study focused
on evaluating anti(myco)bacterial, antifungal, and antiproliferative activities
of seeds of V. canescens subsp. canescens and V. cancescens subsp. pedunculata
(endemic). Ethanol and hexane extracts of seeds of two plants were tested
against three fungal
strains (Candida albicans, Candida tropicalis, and Candida glabrata), two Gram-positive bacterial strains (Bacillus
subtilis and Staphylococcus aureus), three Gram-negative bacterial
strains (Aeromonas hydrophila,
Escherichia coli, and Acinetobacter baumannii), and Mycobacterium tuberculosis H37Rv using broth microdilution method.
Fluconazole, Ethambutol, Ampicillin, and Isoniazid were used as reference drugs.
Antiproliferative effects of various concentrations (200, 100, 50, and 25
μg/mL) of ethanol and hexane extracts were tested against A549 human lung
cancer cell lines using MTT test. The ethanol extracts were found to be more
effective against A. baumannii (62.5
µg/mL MIC value) than Ampicillin (125 µg/mL MIC value). Against A549 human lung
cancer cell lines, 100 µg/mL concentration of ethanol extract of V. canescens subsp. canescens seeds and 200 µg/mL concentration of ethanol extract of V. cancescens subsp. pedunculata seeds were exhibited
statistically lower cell viability levels than control groups (P <0.05). As
a result, V. canescens subsp. canescens and V. cancescens subsp. pedunculata
seeds showed antimicrobial and antiproliferative effects against all tested
microorganisms and A549 human lung cancer cell lines; moreover, ethanol
extracts were found to be more effective than hexane extracts.

Anahtar Kelimeler

Vincetoxicum, Seed, Antimicrobial, Cell viability

Proje Numarası

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Kaynakça

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