Vincetoxicum canescens subsp. canescens ve Vincetoxicum cancescens subsp. pedunculata Tohumlarının Antimikrobiyal ve Antiproliferatif Aktiviteleri

Yıl 2019, Cilt: 9 Sayı: 3, 367 - 375, 25.09.2019

Sevda Güzel , Mahmut Ülger , Yusuf Özay , Önder Yumrutaş , İbrahim Bozgeyik Özkan Sarıkaya

https://doi.org/10.31020/mutftd.594212

Cited By: 4

Öz

Vincetoxicum cinsinin tıbbi özellikleri uzun zamandır bilinmektedir.
Bazı Vincetoxicum türlerinin
yaprakları, kuru tohumları ve rizomları geleneksel tıpta incinme, uyuz, nevroz,
skrofula, sıtma, yara, ateş, rüptür ve eksternal kanserlerin tedavisinde
kullanılmaktadır. Bu çalışma, V.
canescens subsp. canescens ve V. cancescens subsp. pedunculata (endemik) tohumlarının
anti(miko)bakteriyel, antifungal ve antiproliferatif aktivitelerini
değerlendirmeye odaklanmıştır. İki bitkinin tohumlarının etanol ve hekzan
ekstreleri üç fungal suşa (Candida albicans,
Candida tropicalis ve Candida glabrata), iki Gram-pozitif bakteri
suşuna (Bacillus subtilis ve
Staphylococcus aureus), üç Gram-negatif bakteri suşuna (Aeromonas hydrophila, Escherichia coli ve
Acinetobacter baumannii) ve Mycobacterium tuberculosis H37Rv suşuna
karşı Sıvı Mikrodilüsyon Yöntemi kullanılarak test edilmiştir. Referans ilaçlar
olarak Flukonazol, Etambutol, Ampisilin ve İzoniazid kullanılmıştır. Etanol ve
hekzan ekstrelerinin farklı konsantrasyonlarının (200, 100, 50 ve 25 μg/mL)
antiproliferatif etkileri MTT testi kullanılarak A549 insan akciğer kanseri
hücre dizilerine karşı test edilmiştir. Etanol ekstrelerinin A. baumannii'ye (62.5 μg/mL MİK değeri)
karşı Ampisilin'den (125 μg/mL MİK değeri) daha etkili olduğu bulunmuştur. A549
insan akciğer kanseri hücre dizilerine karşı V. canescens subsp. canescens
tohumlarının etanol ekstresinin 100 µg/mL konsantrasyonu ve V. cancescens subsp. pedunculata tohumlarının etanol ekstresinin
200 μg/mL konsantrasyonu, kontrol gruplarına göre istatistiksel olarak daha
düşük hücre canlılık seviyeleri sergilemiştir (P <0.05). Sonuç olarak, V. canescens subsp. canescens ve V. cancescens
subsp. pedunculata tohumları, test
edilen tüm mikroorganizmalara karşı antimikrobiyal ve A549 insan akciğer
kanseri hücre dizilerine karşı antiproliferatif etki göstermiştir; ayrıca,
etanol ekstreleri hekzan ekstrelerinden daha etkili bulunmuştur.

Anahtar Kelimeler

Vincetoxicum, Tohum, Antimikrobiyal

Destekleyen Kurum

-

Proje Numarası

-

Teşekkür

Bitki örneklerinin teşhisinde katkılarından dolayı Dr. Ahmet İlçim'e (Biyoloji Bölümü, Fen-Edebiyat Fakültesi, Mustafa Kemal Üniversitesi) teşekkür ederiz.

Antimicrobial and Antiproliferative Activities of Vincetoxicum canescens subsp. canescens and Vincetoxicum cancescens subsp. pedunculata Seeds

Öz

Medicinal
properties of Vincetoxicum genus have
been known for a long time. Leaves, dry seeds, and rhizomes of some Vincetoxicum species have been used in
folk medicine for the treatment of injuries, scabies, neurosis, scrofula,
malaria, wound, fever, rupture, and external cancers. The present study focused
on evaluating anti(myco)bacterial, antifungal, and antiproliferative activities
of seeds of V. canescens subsp. canescens and V. cancescens subsp. pedunculata
(endemic). Ethanol and hexane extracts of seeds of two plants were tested
against three fungal
strains (Candida albicans, Candida tropicalis, and Candida glabrata), two Gram-positive bacterial strains (Bacillus
subtilis and Staphylococcus aureus), three Gram-negative bacterial
strains (Aeromonas hydrophila,
Escherichia coli, and Acinetobacter baumannii), and Mycobacterium tuberculosis H37Rv using broth microdilution method.
Fluconazole, Ethambutol, Ampicillin, and Isoniazid were used as reference drugs.
Antiproliferative effects of various concentrations (200, 100, 50, and 25
μg/mL) of ethanol and hexane extracts were tested against A549 human lung
cancer cell lines using MTT test. The ethanol extracts were found to be more
effective against A. baumannii (62.5
µg/mL MIC value) than Ampicillin (125 µg/mL MIC value). Against A549 human lung
cancer cell lines, 100 µg/mL concentration of ethanol extract of V. canescens subsp. canescens seeds and 200 µg/mL concentration of ethanol extract of V. cancescens subsp. pedunculata seeds were exhibited
statistically lower cell viability levels than control groups (P <0.05). As
a result, V. canescens subsp. canescens and V. cancescens subsp. pedunculata
seeds showed antimicrobial and antiproliferative effects against all tested
microorganisms and A549 human lung cancer cell lines; moreover, ethanol
extracts were found to be more effective than hexane extracts.

Anahtar Kelimeler

Vincetoxicum, Seed, Antimicrobial, Cell viability

Proje Numarası

-

Kaynakça

• 1. Pașca C, et al. Medicinal plants based products tested on pathogens isolated from Mastitis Milk. Molecules 2017;22:1473-1489.
• 2. Siddiqui W, et al. Antimicrobial properties of teas and their extracts in vitro. Crit Rev Food Sci Nutr 2016;56(9):1428-1439.
• 3. Ngezahayo J, et al. In vitro study of five herbs used against microbial infections in Burundi. Phytother Res 2017;31:1571-1578.
• 4. Guzel S, et al. Wound healing properties, antimicrobial and antioxidant activities of Salvia kronenburgii Rech. f. and Salvia euphratica Montbret, Aucher & Rech. f. var. euphratica on excision and incision wound models in diabetic rats. Biomed Pharmacother 2019;111:1260-1276.
• 5. Anadozie, SO, et al. Bryophyllum pinnatum inhibits arginase II activity and prevents oxidative damage occasioned by carbon tetrachloride (CCl4) in rats. Biomed Pharmacother 2018;101:8-13.
• 6. Leimu R. Variation in the mating system of Vincetoxicum hirundinaria (Asclepiadaceae) in peripherial island populations. Ann Bot 2004;93:107-113.
• 7. Liede S. Cynanchum-Rhodostegiella-Vincetoxicum-Tylophora (Asclepiadaceae): new considerations on an old problem. Taxon 1996;45:193-211.
• 8. Sliumpaite I, et al. Antioxidant properties and phenolic composition of swallow-wort (Vincetoxicum lutea L.) leaves. Ind Crops Prod 2013;45:74-82.
• 9. DiTommaso A, Lawlor FM, Darbyshire SJ. The biology of invasive alien plants in Canada 2. Cynanchum rossicum (Kleopow) Borhidi (=Vincetoxicum rossicum (Kleopow) Barbar.) and Cynanchum louseae (L.) Kartesz & Gandhi (= Vincetoxicum nigrum (L.) Moench). Can J Plant Sci 2004;85:243-263.
• 10. Mansoor A, et al. Antiprotozoal activities of Vincetoxicum stocksii and Carum copticum. Bangladesh J Pharmacol 2011;6:51-54.
• 11. Nowak R, Kisiel W. Hancokinol from Vincetoxicum officinale. Fitoterapia 2000;71:584-586.
• 12. Lavault M, Richomme P, Bruneton J. New phenantroindolizidine N-oxides alkaloids isolated from Vincetoxicum hirundinaria Medic. Pharm Acta Helv 1994;68:225-227.
• 13. Coassini-Lokar L, Poldini L. Herbal remedies in the traditional medicine of the Venezia Giulia Region (North East Italy). J Ethnopharmacol 1988;22:231-278.
• 14. Baytop T, Türkiye’de bitkiler ile tedavi geçmişte ve bugün. (İlaveli 2. Baskı), İstanbul Üniversitesi Eczacılık Fakültesi, Nobel Tıp Kitap Evleri, İstanbul; 1984.pp:372.
• 15. Altundag E, Ozturk M. Ethnomedicinal studies on the plant resources of East Anatolia, Turkey. Procedia Soc Behav Sci 2011;19:756-777.
• 16. Staerk D, et al. In vitro cytotoxic activity of phenantroindolizidine alkaloids from Cynanchum vincetoxicum and Tylophora tanakae against drug-sensitive and multidrug-resistant cancer cells. J Nat Prod 2002;65:1299-1302.
• 17. Zaidi MA, Crow JSA. Biologically active traditional medicinal herbs from Balochistan, Pakistan J Ethnopharmacol 2005;96:331-334.
• 18. Guzel S, et al. Evaluation of antimicrobial activity of five Vincetoxicum taxa growing in Turkey. Mamar Pharm J 2018;22(3):365-373.
• 19. Guzel S, Ulger M, Kokdil G. Anti(myco)bacterial and antifungal activities of seed pods of four Vincetoxicum taxa growing in Turkey. International Journal of Scientific and Technological Research 2018;4(3):27-38.
• 20. Guzel S, et al. Phytochemical composition and antifeedant activity of five Vincetoxicum taxa against Spodoptera littoralis and Leptinotarsa decemlineata. Mamar Pharm J 2017;21(4):872-880.
• 21. Guzel S, Pavela R, Kokdil G. Phytochemistry and antifeedant activity of root extracts from some Vincetoxicum taxa against Leptinotarsa decemlineata and Spodoptera littoralis. JBiopest 2015;8(2):128-140.
• 22. Browicz K. Vincetoxicum N.M. Wolf. In: Davis, P.H. (Eds), Flora of Turkey and the East Aegean Islands. Vol 6, Edinburgh; 1978.pp:163-173.
• 23. Guzel S, Pavela R, Kokdil G. Evaluation of antifungal effect of different polarity extracts from five Vincetoxicum taxa against Aspergillus fumigatus. International Anatolian Academic Online Journal/Health Science 2015;3(2): 1-9.
• 24. NCCLS. Reference method for broth dilution antifungal susceptibility testing of yeasts; approved standard, second ed. NCCLS document M27-A2 (ISBN 1-56238-469-4); Wayne; Pennsylvania; 2002.
• 25. Yumrutas O, et al. Association between anti-proliferative activity of Evernia prunastri with the cellular apoptotic pathway. Cumhuriyet Sci J 2017;38(3):516-524.
• 26. Bazzaz F, Haririzadeh G. Screening of Iranian plants for antimicrobial activity. Pharm Biol 2003;41(8):573-583.
• 27. Mogg C, et al. Test of the antibiotic properties of the invasive vine Vincetoxicum rossicum against bacteria, fungi and insects. Biochem Syst Ecol 2008;36:383-391.
• 28. Bajpai VK. Antimicrobial bioactive compounds from marine algae: a mini review. Indian J Geomarine Sci 2016;45(9):1076-1085.
• 29. Alimpić A, et al. Biological activities and chemical composition of Salvia amplexicaulis Lam. extracts. Ind Crops Prod 2017;105:1-9.
• 30. Lean SS, Yeo CC. Small, enigmatic plasmids of the nosocomial pathogen, Acinetobacter baumannii: good, bad, who knows? Front Microbiol 2017;8:1547-1555.
• 31. Shamsizadeh Z, et al. Detection of antibiotic resistant Acinetobacter baumannii in various hospital environments: potential sources for transmission of Acinetobacter infections. Environ Health Prev Med 2017;22(44):1-7.
• 32. Sak K, et al. Cytotoxic action of methylquercetins in human lung adenocarcinoma cells. Oncol Lett 2018;15:1973-1978.
• 33. Staerk D, et al. Cytotoxic activity of some phenanthroindolizidine N-oxide alkaloids from Cynanchum vincetoxicum. J Nat Prod 2000;63:1584-1586.
• 34. Kowalczyk D, et al. The phenolic content and antioxidant activity of the aqueous and hydroalcoholic extracts of hops and their pellets. J Inst Brew 2013;119(3):103-110.