Araştırma Makalesi
PDF Mendeley EndNote BibTex Kaynak Göster

Antimicrobial and Antiproliferative Activities of Vincetoxicum canescens subsp. canescens and Vincetoxicum cancescens subsp. pedunculata Seeds

Yıl 2019, Cilt 9, Sayı 3, 367 - 375, 25.09.2019
https://doi.org/10.31020/mutftd.594212

Öz

Medicinal properties of Vincetoxicum genus have been known for a long time. Leaves, dry seeds, and rhizomes of some Vincetoxicum species have been used in folk medicine for the treatment of injuries, scabies, neurosis, scrofula, malaria, wound, fever, rupture, and external cancers. The present study focused on evaluating anti(myco)bacterial, antifungal, and antiproliferative activities of seeds of V. canescens subsp. canescens and V. cancescens subsp. pedunculata (endemic). Ethanol and hexane extracts of seeds of two plants were tested against three fungal strains (Candida albicans, Candida tropicalis, and Candida glabrata), two Gram-positive bacterial strains (Bacillus subtilis and Staphylococcus aureus), three Gram-negative bacterial strains (Aeromonas hydrophila, Escherichia coli, and Acinetobacter baumannii), and Mycobacterium tuberculosis H37Rv using broth microdilution method. Fluconazole, Ethambutol, Ampicillin, and Isoniazid were used as reference drugs. Antiproliferative effects of various concentrations (200, 100, 50, and 25 μg/mL) of ethanol and hexane extracts were tested against A549 human lung cancer cell lines using MTT test. The ethanol extracts were found to be more effective against A. baumannii (62.5 µg/mL MIC value) than Ampicillin (125 µg/mL MIC value). Against A549 human lung cancer cell lines, 100 µg/mL concentration of ethanol extract of V. canescens subsp. canescens seeds and 200 µg/mL concentration of ethanol extract of V. cancescens subsp. pedunculata seeds were exhibited statistically lower cell viability levels than control groups (P <0.05). As a result, V. canescens subsp. canescens and V. cancescens subsp. pedunculata seeds showed antimicrobial and antiproliferative effects against all tested microorganisms and A549 human lung cancer cell lines; moreover, ethanol extracts were found to be more effective than hexane extracts.

Kaynakça

  • 1. Pașca C, et al. Medicinal plants based products tested on pathogens isolated from Mastitis Milk. Molecules 2017;22:1473-1489.
  • 2. Siddiqui W, et al. Antimicrobial properties of teas and their extracts in vitro. Crit Rev Food Sci Nutr 2016;56(9):1428-1439.
  • 3. Ngezahayo J, et al. In vitro study of five herbs used against microbial infections in Burundi. Phytother Res 2017;31:1571-1578.
  • 4. Guzel S, et al. Wound healing properties, antimicrobial and antioxidant activities of Salvia kronenburgii Rech. f. and Salvia euphratica Montbret, Aucher & Rech. f. var. euphratica on excision and incision wound models in diabetic rats. Biomed Pharmacother 2019;111:1260-1276.
  • 5. Anadozie, SO, et al. Bryophyllum pinnatum inhibits arginase II activity and prevents oxidative damage occasioned by carbon tetrachloride (CCl4) in rats. Biomed Pharmacother 2018;101:8-13.
  • 6. Leimu R. Variation in the mating system of Vincetoxicum hirundinaria (Asclepiadaceae) in peripherial island populations. Ann Bot 2004;93:107-113.
  • 7. Liede S. Cynanchum-Rhodostegiella-Vincetoxicum-Tylophora (Asclepiadaceae): new considerations on an old problem. Taxon 1996;45:193-211.
  • 8. Sliumpaite I, et al. Antioxidant properties and phenolic composition of swallow-wort (Vincetoxicum lutea L.) leaves. Ind Crops Prod 2013;45:74-82.
  • 9. DiTommaso A, Lawlor FM, Darbyshire SJ. The biology of invasive alien plants in Canada 2. Cynanchum rossicum (Kleopow) Borhidi (=Vincetoxicum rossicum (Kleopow) Barbar.) and Cynanchum louseae (L.) Kartesz & Gandhi (= Vincetoxicum nigrum (L.) Moench). Can J Plant Sci 2004;85:243-263.
  • 10. Mansoor A, et al. Antiprotozoal activities of Vincetoxicum stocksii and Carum copticum. Bangladesh J Pharmacol 2011;6:51-54.
  • 11. Nowak R, Kisiel W. Hancokinol from Vincetoxicum officinale. Fitoterapia 2000;71:584-586.
  • 12. Lavault M, Richomme P, Bruneton J. New phenantroindolizidine N-oxides alkaloids isolated from Vincetoxicum hirundinaria Medic. Pharm Acta Helv 1994;68:225-227.
  • 13. Coassini-Lokar L, Poldini L. Herbal remedies in the traditional medicine of the Venezia Giulia Region (North East Italy). J Ethnopharmacol 1988;22:231-278.
  • 14. Baytop T, Türkiye’de bitkiler ile tedavi geçmişte ve bugün. (İlaveli 2. Baskı), İstanbul Üniversitesi Eczacılık Fakültesi, Nobel Tıp Kitap Evleri, İstanbul; 1984.pp:372.
  • 15. Altundag E, Ozturk M. Ethnomedicinal studies on the plant resources of East Anatolia, Turkey. Procedia Soc Behav Sci 2011;19:756-777.
  • 16. Staerk D, et al. In vitro cytotoxic activity of phenantroindolizidine alkaloids from Cynanchum vincetoxicum and Tylophora tanakae against drug-sensitive and multidrug-resistant cancer cells. J Nat Prod 2002;65:1299-1302.
  • 17. Zaidi MA, Crow JSA. Biologically active traditional medicinal herbs from Balochistan, Pakistan J Ethnopharmacol 2005;96:331-334.
  • 18. Guzel S, et al. Evaluation of antimicrobial activity of five Vincetoxicum taxa growing in Turkey. Mamar Pharm J 2018;22(3):365-373.
  • 19. Guzel S, Ulger M, Kokdil G. Anti(myco)bacterial and antifungal activities of seed pods of four Vincetoxicum taxa growing in Turkey. International Journal of Scientific and Technological Research 2018;4(3):27-38.
  • 20. Guzel S, et al. Phytochemical composition and antifeedant activity of five Vincetoxicum taxa against Spodoptera littoralis and Leptinotarsa decemlineata. Mamar Pharm J 2017;21(4):872-880.
  • 21. Guzel S, Pavela R, Kokdil G. Phytochemistry and antifeedant activity of root extracts from some Vincetoxicum taxa against Leptinotarsa decemlineata and Spodoptera littoralis. JBiopest 2015;8(2):128-140.
  • 22. Browicz K. Vincetoxicum N.M. Wolf. In: Davis, P.H. (Eds), Flora of Turkey and the East Aegean Islands. Vol 6, Edinburgh; 1978.pp:163-173.
  • 23. Guzel S, Pavela R, Kokdil G. Evaluation of antifungal effect of different polarity extracts from five Vincetoxicum taxa against Aspergillus fumigatus. International Anatolian Academic Online Journal/Health Science 2015;3(2): 1-9.
  • 24. NCCLS. Reference method for broth dilution antifungal susceptibility testing of yeasts; approved standard, second ed. NCCLS document M27-A2 (ISBN 1-56238-469-4); Wayne; Pennsylvania; 2002.
  • 25. Yumrutas O, et al. Association between anti-proliferative activity of Evernia prunastri with the cellular apoptotic pathway. Cumhuriyet Sci J 2017;38(3):516-524.
  • 26. Bazzaz F, Haririzadeh G. Screening of Iranian plants for antimicrobial activity. Pharm Biol 2003;41(8):573-583.
  • 27. Mogg C, et al. Test of the antibiotic properties of the invasive vine Vincetoxicum rossicum against bacteria, fungi and insects. Biochem Syst Ecol 2008;36:383-391.
  • 28. Bajpai VK. Antimicrobial bioactive compounds from marine algae: a mini review. Indian J Geomarine Sci 2016;45(9):1076-1085.
  • 29. Alimpić A, et al. Biological activities and chemical composition of Salvia amplexicaulis Lam. extracts. Ind Crops Prod 2017;105:1-9.
  • 30. Lean SS, Yeo CC. Small, enigmatic plasmids of the nosocomial pathogen, Acinetobacter baumannii: good, bad, who knows? Front Microbiol 2017;8:1547-1555.
  • 31. Shamsizadeh Z, et al. Detection of antibiotic resistant Acinetobacter baumannii in various hospital environments: potential sources for transmission of Acinetobacter infections. Environ Health Prev Med 2017;22(44):1-7.
  • 32. Sak K, et al. Cytotoxic action of methylquercetins in human lung adenocarcinoma cells. Oncol Lett 2018;15:1973-1978.
  • 33. Staerk D, et al. Cytotoxic activity of some phenanthroindolizidine N-oxide alkaloids from Cynanchum vincetoxicum. J Nat Prod 2000;63:1584-1586.
  • 34. Kowalczyk D, et al. The phenolic content and antioxidant activity of the aqueous and hydroalcoholic extracts of hops and their pellets. J Inst Brew 2013;119(3):103-110.

Vincetoxicum canescens subsp. canescens ve Vincetoxicum cancescens subsp. pedunculata Tohumlarının Antimikrobiyal ve Antiproliferatif Aktiviteleri

Yıl 2019, Cilt 9, Sayı 3, 367 - 375, 25.09.2019
https://doi.org/10.31020/mutftd.594212

Öz

Vincetoxicum cinsinin tıbbi özellikleri uzun zamandır bilinmektedir. Bazı Vincetoxicum türlerinin yaprakları, kuru tohumları ve rizomları geleneksel tıpta incinme, uyuz, nevroz, skrofula, sıtma, yara, ateş, rüptür ve eksternal kanserlerin tedavisinde kullanılmaktadır. Bu çalışma, V. canescens subsp. canescens ve V. cancescens subsp. pedunculata (endemik) tohumlarının anti(miko)bakteriyel, antifungal ve antiproliferatif aktivitelerini değerlendirmeye odaklanmıştır. İki bitkinin tohumlarının etanol ve hekzan ekstreleri üç fungal suşa (Candida albicans, Candida tropicalis ve Candida glabrata), iki Gram-pozitif bakteri suşuna (Bacillus subtilis ve Staphylococcus aureus), üç Gram-negatif bakteri suşuna (Aeromonas hydrophila, Escherichia coli ve Acinetobacter baumannii) ve Mycobacterium tuberculosis H37Rv suşuna karşı Sıvı Mikrodilüsyon Yöntemi kullanılarak test edilmiştir. Referans ilaçlar olarak Flukonazol, Etambutol, Ampisilin ve İzoniazid kullanılmıştır. Etanol ve hekzan ekstrelerinin farklı konsantrasyonlarının (200, 100, 50 ve 25 μg/mL) antiproliferatif etkileri MTT testi kullanılarak A549 insan akciğer kanseri hücre dizilerine karşı test edilmiştir. Etanol ekstrelerinin A. baumannii'ye (62.5 μg/mL MİK değeri) karşı Ampisilin'den (125 μg/mL MİK değeri) daha etkili olduğu bulunmuştur. A549 insan akciğer kanseri hücre dizilerine karşı V. canescens subsp. canescens tohumlarının etanol ekstresinin 100 µg/mL konsantrasyonu ve V. cancescens subsp. pedunculata tohumlarının etanol ekstresinin 200 μg/mL konsantrasyonu, kontrol gruplarına göre istatistiksel olarak daha düşük hücre canlılık seviyeleri sergilemiştir (P <0.05). Sonuç olarak, V. canescens subsp. canescens ve V. cancescens subsp. pedunculata tohumları, test edilen tüm mikroorganizmalara karşı antimikrobiyal ve A549 insan akciğer kanseri hücre dizilerine karşı antiproliferatif etki göstermiştir; ayrıca, etanol ekstreleri hekzan ekstrelerinden daha etkili bulunmuştur.

Kaynakça

  • 1. Pașca C, et al. Medicinal plants based products tested on pathogens isolated from Mastitis Milk. Molecules 2017;22:1473-1489.
  • 2. Siddiqui W, et al. Antimicrobial properties of teas and their extracts in vitro. Crit Rev Food Sci Nutr 2016;56(9):1428-1439.
  • 3. Ngezahayo J, et al. In vitro study of five herbs used against microbial infections in Burundi. Phytother Res 2017;31:1571-1578.
  • 4. Guzel S, et al. Wound healing properties, antimicrobial and antioxidant activities of Salvia kronenburgii Rech. f. and Salvia euphratica Montbret, Aucher & Rech. f. var. euphratica on excision and incision wound models in diabetic rats. Biomed Pharmacother 2019;111:1260-1276.
  • 5. Anadozie, SO, et al. Bryophyllum pinnatum inhibits arginase II activity and prevents oxidative damage occasioned by carbon tetrachloride (CCl4) in rats. Biomed Pharmacother 2018;101:8-13.
  • 6. Leimu R. Variation in the mating system of Vincetoxicum hirundinaria (Asclepiadaceae) in peripherial island populations. Ann Bot 2004;93:107-113.
  • 7. Liede S. Cynanchum-Rhodostegiella-Vincetoxicum-Tylophora (Asclepiadaceae): new considerations on an old problem. Taxon 1996;45:193-211.
  • 8. Sliumpaite I, et al. Antioxidant properties and phenolic composition of swallow-wort (Vincetoxicum lutea L.) leaves. Ind Crops Prod 2013;45:74-82.
  • 9. DiTommaso A, Lawlor FM, Darbyshire SJ. The biology of invasive alien plants in Canada 2. Cynanchum rossicum (Kleopow) Borhidi (=Vincetoxicum rossicum (Kleopow) Barbar.) and Cynanchum louseae (L.) Kartesz & Gandhi (= Vincetoxicum nigrum (L.) Moench). Can J Plant Sci 2004;85:243-263.
  • 10. Mansoor A, et al. Antiprotozoal activities of Vincetoxicum stocksii and Carum copticum. Bangladesh J Pharmacol 2011;6:51-54.
  • 11. Nowak R, Kisiel W. Hancokinol from Vincetoxicum officinale. Fitoterapia 2000;71:584-586.
  • 12. Lavault M, Richomme P, Bruneton J. New phenantroindolizidine N-oxides alkaloids isolated from Vincetoxicum hirundinaria Medic. Pharm Acta Helv 1994;68:225-227.
  • 13. Coassini-Lokar L, Poldini L. Herbal remedies in the traditional medicine of the Venezia Giulia Region (North East Italy). J Ethnopharmacol 1988;22:231-278.
  • 14. Baytop T, Türkiye’de bitkiler ile tedavi geçmişte ve bugün. (İlaveli 2. Baskı), İstanbul Üniversitesi Eczacılık Fakültesi, Nobel Tıp Kitap Evleri, İstanbul; 1984.pp:372.
  • 15. Altundag E, Ozturk M. Ethnomedicinal studies on the plant resources of East Anatolia, Turkey. Procedia Soc Behav Sci 2011;19:756-777.
  • 16. Staerk D, et al. In vitro cytotoxic activity of phenantroindolizidine alkaloids from Cynanchum vincetoxicum and Tylophora tanakae against drug-sensitive and multidrug-resistant cancer cells. J Nat Prod 2002;65:1299-1302.
  • 17. Zaidi MA, Crow JSA. Biologically active traditional medicinal herbs from Balochistan, Pakistan J Ethnopharmacol 2005;96:331-334.
  • 18. Guzel S, et al. Evaluation of antimicrobial activity of five Vincetoxicum taxa growing in Turkey. Mamar Pharm J 2018;22(3):365-373.
  • 19. Guzel S, Ulger M, Kokdil G. Anti(myco)bacterial and antifungal activities of seed pods of four Vincetoxicum taxa growing in Turkey. International Journal of Scientific and Technological Research 2018;4(3):27-38.
  • 20. Guzel S, et al. Phytochemical composition and antifeedant activity of five Vincetoxicum taxa against Spodoptera littoralis and Leptinotarsa decemlineata. Mamar Pharm J 2017;21(4):872-880.
  • 21. Guzel S, Pavela R, Kokdil G. Phytochemistry and antifeedant activity of root extracts from some Vincetoxicum taxa against Leptinotarsa decemlineata and Spodoptera littoralis. JBiopest 2015;8(2):128-140.
  • 22. Browicz K. Vincetoxicum N.M. Wolf. In: Davis, P.H. (Eds), Flora of Turkey and the East Aegean Islands. Vol 6, Edinburgh; 1978.pp:163-173.
  • 23. Guzel S, Pavela R, Kokdil G. Evaluation of antifungal effect of different polarity extracts from five Vincetoxicum taxa against Aspergillus fumigatus. International Anatolian Academic Online Journal/Health Science 2015;3(2): 1-9.
  • 24. NCCLS. Reference method for broth dilution antifungal susceptibility testing of yeasts; approved standard, second ed. NCCLS document M27-A2 (ISBN 1-56238-469-4); Wayne; Pennsylvania; 2002.
  • 25. Yumrutas O, et al. Association between anti-proliferative activity of Evernia prunastri with the cellular apoptotic pathway. Cumhuriyet Sci J 2017;38(3):516-524.
  • 26. Bazzaz F, Haririzadeh G. Screening of Iranian plants for antimicrobial activity. Pharm Biol 2003;41(8):573-583.
  • 27. Mogg C, et al. Test of the antibiotic properties of the invasive vine Vincetoxicum rossicum against bacteria, fungi and insects. Biochem Syst Ecol 2008;36:383-391.
  • 28. Bajpai VK. Antimicrobial bioactive compounds from marine algae: a mini review. Indian J Geomarine Sci 2016;45(9):1076-1085.
  • 29. Alimpić A, et al. Biological activities and chemical composition of Salvia amplexicaulis Lam. extracts. Ind Crops Prod 2017;105:1-9.
  • 30. Lean SS, Yeo CC. Small, enigmatic plasmids of the nosocomial pathogen, Acinetobacter baumannii: good, bad, who knows? Front Microbiol 2017;8:1547-1555.
  • 31. Shamsizadeh Z, et al. Detection of antibiotic resistant Acinetobacter baumannii in various hospital environments: potential sources for transmission of Acinetobacter infections. Environ Health Prev Med 2017;22(44):1-7.
  • 32. Sak K, et al. Cytotoxic action of methylquercetins in human lung adenocarcinoma cells. Oncol Lett 2018;15:1973-1978.
  • 33. Staerk D, et al. Cytotoxic activity of some phenanthroindolizidine N-oxide alkaloids from Cynanchum vincetoxicum. J Nat Prod 2000;63:1584-1586.
  • 34. Kowalczyk D, et al. The phenolic content and antioxidant activity of the aqueous and hydroalcoholic extracts of hops and their pellets. J Inst Brew 2013;119(3):103-110.

Ayrıntılar

Birincil Dil Türkçe
Konular Tıp
Bölüm Araştırma Makalesi
Yazarlar

Sevda GÜZEL (Sorumlu Yazar)
MERSİN ÜNİVERSİTESİ
0000-0002-6642-5824
Türkiye


Mahmut ÜLGER
MERSİN ÜNİVERSİTESİ
0000-0001-6649-4195
Türkiye


Yusuf ÖZAY
ADIYAMAN ÜNİVERSİTESİ
0000-0003-3855-6197
Türkiye


Önder YUMRUTAŞ
ADIYAMAN ÜNİVERSİTESİ
0000-0001-9657-8306
Türkiye


İbrahim BOZGEYİK Bu kişi benim
ADIYAMAN ÜNİVERSİTESİ
0000-0003-1483-2580
Türkiye


Özkan SARIKAYA
KIRŞEHİR AHİ EVRAN ÜNİVERSİTESİ
0000-0002-1641-457X
Türkiye

Destekleyen Kurum -
Proje Numarası -
Teşekkür Bitki örneklerinin teşhisinde katkılarından dolayı Dr. Ahmet İlçim'e (Biyoloji Bölümü, Fen-Edebiyat Fakültesi, Mustafa Kemal Üniversitesi) teşekkür ederiz.
Yayımlanma Tarihi 25 Eylül 2019
Başvuru Tarihi 19 Temmuz 2019
Kabul Tarihi 2 Eylül 2019
Yayınlandığı Sayı Yıl 2019, Cilt 9, Sayı 3

Kaynak Göster

Bibtex @araştırma makalesi { mutftd594212, journal = {Mersin Üniversitesi Tıp Fakültesi Lokman Hekim Tıp Tarihi ve Folklorik Tıp Dergisi}, issn = {1309-761X}, eissn = {1309-8004}, address = {lokman@mersin.edu.tr}, publisher = {Mersin Üniversitesi}, year = {2019}, volume = {9}, number = {3}, pages = {367 - 375}, doi = {10.31020/mutftd.594212}, title = {Vincetoxicum canescens subsp. canescens ve Vincetoxicum cancescens subsp. pedunculata Tohumlarının Antimikrobiyal ve Antiproliferatif Aktiviteleri}, key = {cite}, author = {Güzel, Sevda and Ülger, Mahmut and Özay, Yusuf and Yumrutaş, Önder and Bozgeyik, İbrahim and Sarıkaya, Özkan} }
APA Güzel, S. , Ülger, M. , Özay, Y. , Yumrutaş, Ö. , Bozgeyik, İ. & Sarıkaya, Ö. (2019). Vincetoxicum canescens subsp. canescens ve Vincetoxicum cancescens subsp. pedunculata Tohumlarının Antimikrobiyal ve Antiproliferatif Aktiviteleri . Mersin Üniversitesi Tıp Fakültesi Lokman Hekim Tıp Tarihi ve Folklorik Tıp Dergisi , 9 (3) , 367-375 . DOI: 10.31020/mutftd.594212
MLA Güzel, S. , Ülger, M. , Özay, Y. , Yumrutaş, Ö. , Bozgeyik, İ. , Sarıkaya, Ö. "Vincetoxicum canescens subsp. canescens ve Vincetoxicum cancescens subsp. pedunculata Tohumlarının Antimikrobiyal ve Antiproliferatif Aktiviteleri" . Mersin Üniversitesi Tıp Fakültesi Lokman Hekim Tıp Tarihi ve Folklorik Tıp Dergisi 9 (2019 ): 367-375 <https://dergipark.org.tr/tr/pub/mutftd/issue/48799/594212>
Chicago Güzel, S. , Ülger, M. , Özay, Y. , Yumrutaş, Ö. , Bozgeyik, İ. , Sarıkaya, Ö. "Vincetoxicum canescens subsp. canescens ve Vincetoxicum cancescens subsp. pedunculata Tohumlarının Antimikrobiyal ve Antiproliferatif Aktiviteleri". Mersin Üniversitesi Tıp Fakültesi Lokman Hekim Tıp Tarihi ve Folklorik Tıp Dergisi 9 (2019 ): 367-375
RIS TY - JOUR T1 - Vincetoxicum canescens subsp. canescens ve Vincetoxicum cancescens subsp. pedunculata Tohumlarının Antimikrobiyal ve Antiproliferatif Aktiviteleri AU - Sevda Güzel , Mahmut Ülger , Yusuf Özay , Önder Yumrutaş , İbrahim Bozgeyik , Özkan Sarıkaya Y1 - 2019 PY - 2019 N1 - doi: 10.31020/mutftd.594212 DO - 10.31020/mutftd.594212 T2 - Mersin Üniversitesi Tıp Fakültesi Lokman Hekim Tıp Tarihi ve Folklorik Tıp Dergisi JF - Journal JO - JOR SP - 367 EP - 375 VL - 9 IS - 3 SN - 1309-761X-1309-8004 M3 - doi: 10.31020/mutftd.594212 UR - https://doi.org/10.31020/mutftd.594212 Y2 - 2019 ER -
EndNote %0 Mersin Üniversitesi Tıp Fakültesi Lokman Hekim Tıp Tarihi ve Folklorik Tıp Dergisi Vincetoxicum canescens subsp. canescens ve Vincetoxicum cancescens subsp. pedunculata Tohumlarının Antimikrobiyal ve Antiproliferatif Aktiviteleri %A Sevda Güzel , Mahmut Ülger , Yusuf Özay , Önder Yumrutaş , İbrahim Bozgeyik , Özkan Sarıkaya %T Vincetoxicum canescens subsp. canescens ve Vincetoxicum cancescens subsp. pedunculata Tohumlarının Antimikrobiyal ve Antiproliferatif Aktiviteleri %D 2019 %J Mersin Üniversitesi Tıp Fakültesi Lokman Hekim Tıp Tarihi ve Folklorik Tıp Dergisi %P 1309-761X-1309-8004 %V 9 %N 3 %R doi: 10.31020/mutftd.594212 %U 10.31020/mutftd.594212
ISNAD Güzel, Sevda , Ülger, Mahmut , Özay, Yusuf , Yumrutaş, Önder , Bozgeyik, İbrahim , Sarıkaya, Özkan . "Vincetoxicum canescens subsp. canescens ve Vincetoxicum cancescens subsp. pedunculata Tohumlarının Antimikrobiyal ve Antiproliferatif Aktiviteleri". Mersin Üniversitesi Tıp Fakültesi Lokman Hekim Tıp Tarihi ve Folklorik Tıp Dergisi 9 / 3 (Eylül 2019): 367-375 . https://doi.org/10.31020/mutftd.594212
AMA Güzel S. , Ülger M. , Özay Y. , Yumrutaş Ö. , Bozgeyik İ. , Sarıkaya Ö. Vincetoxicum canescens subsp. canescens ve Vincetoxicum cancescens subsp. pedunculata Tohumlarının Antimikrobiyal ve Antiproliferatif Aktiviteleri. Mersin Üniversitesi Tıp Fakültesi Lokman Hekim Tıp Tarihi ve Folklorik Tıp Dergisi. 2019; 9(3): 367-375.
Vancouver Güzel S. , Ülger M. , Özay Y. , Yumrutaş Ö. , Bozgeyik İ. , Sarıkaya Ö. Vincetoxicum canescens subsp. canescens ve Vincetoxicum cancescens subsp. pedunculata Tohumlarının Antimikrobiyal ve Antiproliferatif Aktiviteleri. Mersin Üniversitesi Tıp Fakültesi Lokman Hekim Tıp Tarihi ve Folklorik Tıp Dergisi. 2019; 9(3): 367-375.
IEEE S. Güzel , M. Ülger , Y. Özay , Ö. Yumrutaş , İ. Bozgeyik ve Ö. Sarıkaya , "Vincetoxicum canescens subsp. canescens ve Vincetoxicum cancescens subsp. pedunculata Tohumlarının Antimikrobiyal ve Antiproliferatif Aktiviteleri", Mersin Üniversitesi Tıp Fakültesi Lokman Hekim Tıp Tarihi ve Folklorik Tıp Dergisi, c. 9, sayı. 3, ss. 367-375, Eyl. 2019, doi:10.31020/mutftd.594212
Creative Commons Lisansı
                                                                              Bu eser Creative Commons Atıf-GayriTicari 4.0 Uluslararası Lisansı ile lisanslanmıştır.
                                            

Mersin Üniversitesi Tıp Fakültesi’nin süreli bilimsel yayınıdır. Kaynak gösterilmeden kullanılamaz.  Makalelerin sorumlulukları yazarlara aittir 

Kapak 

Ayşegül Tuğuz

İlter Uzel’inDioskorides ve Öğrencisi adlı eserinden 

Adres

Mersin Üniversitesi Tıp Fakültesi Tıp Tarihi ve Etik  Anabilim Dalı Çiftlikköy Kampüsü

Yenişehir/ Mersin